Chronobiology International最新文献

{"title":"Morningness-eveningness scale for children: Difficulties in establishing reference score intervals by age and sex throughout adolescence.","authors":"Jeanny Joana Rodrigues Alves de Santana, Jefferson Souza Santos, Fernando Mazzilli Louzada, Sabine Pompeia","doi":"10.1080/07420528.2025.2532798","DOIUrl":"https://doi.org/10.1080/07420528.2025.2532798","url":null,"abstract":"<p><p>As adolescents become older, they tend to prefer sleeping and waking later due to biological maturation and social/environmental changes. Higher evening preferences relate to risks of developing physical and mental ill-health and/or academic underachievement. To identify individuals who are more vulnerable to these negative outcomes, prior studies have mostly established percentile cutoff scores in questionnaires of morningness-eveningness (M-E) preferences, a method that leads to biased estimates, and/or used linear statistical methods, which do not consider that M-E can fit other types of distribution. We reanalyzed cross-sectional data of 1815 10-18-year-old Brazilians who filled in the most popular circadian preference scale, the M-E Scale for Children (MESC). Age/sex MESC sum raw score differences were analyzed using correlations, general linear models and 44 different curve fittings based on fractional polynomials and exponential data transformation. We found a very slight correlation and general linear increase in eveningness with age, but none of the 44 tested curve fit patterns reliably explained score changes across ages, being highly variable at all ages. Hence, establishing MESC reference score intervals by age/sex is of little practical value. We discuss other factors combined with MESC scores that may help identify adolescents at risk of circadian-related problem.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>NR1D1 (REVERBalpha)</i> may be novel candidate gene for coronary artery disease in men: Differential effects of <i>NR1D1</i> polymorphisms by gender.","authors":"Rukiye Yesil, Cagatay Aydogan, Gulcin Ozkara, Bengu Tokat, Ahmet Yildiz, Abdullah Omer Ebeoglu, Mehmet Fatih Seyhan, Oguz Ozturk, Hulya Yilmaz-Aydogan","doi":"10.1080/07420528.2025.2532797","DOIUrl":"https://doi.org/10.1080/07420528.2025.2532797","url":null,"abstract":"<p><p>Circadian rhythms are strongly linked to cardiometabolic syndromes such as coronary artery disease (CAD). <i>NR1D1(REVERBalpha)</i> regulates lipid metabolism and circadian clock. This study investigated possible associations between the <i>NR1D1</i> rs2314339 C > T and rs72836608 C > A polymorphisms and metabolic parameters in 126 CAD patients and 125 controls. Allelic discrimination was performed by Real-Time PCR using TaqMan Genotyping Assays. The rs2314339-CC and rs72836608-AA genotypes were associated with an increased risk of CAD (<i>p</i> < 0.05), which varied according to cardiovascular risk factors. The rs72836608-A allele and rs2314339-CC genotypes were associated with an increased risk of CAD in healthy-weight, non-diabetic, normolipidemic, and male patients (<i>p</i> < 0.05). Additionally, the rs72836608-A allele was associated with an elevated risk of CAD in patients with hypertension (<i>p</i> = 0.016). Subgroup analysis by gender showed that the rs72836608-A allele (<i>p</i> = 0.018), the rs2314339-CC genotype (<i>p</i> = 0.008), hyperlipidemia (<i>p</i> = 0.001), hypertension (<i>p</i> = 0.001), and type 2 diabetes mellitus (T2DM) (<i>p</i> = 0.001) were associated with an increased risk of CAD in men. Nevertheless, the presence of hypertension (<i>p</i> = 0.008), hyperlipidemia (<i>p</i> = 0.025), and T2DM (<i>p</i> = 0.001) were significantly associated with CAD risk in the females. Multivariate regression analysis revealed that the rs72836608-A allele (<i>p</i> = 0.034), male gender (<i>p</i> = 0.01), hyperlipidemia (<i>p</i> = 0.008), hypertension (<i>p</i> = 0.001), and T2DM (<i>p</i> = 0.001) were associated with an increased risk for CAD in the overall cohort. The findings suggest that both polymorphisms may be associated with an increased risk of CAD, particularly in men, and may be influenced by factors including age and other cardiovascular risk factors.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-13"},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External validation of the Japanese version of the reduced morningness-eveningness questionnaire (rMEQ) score using dim light melatonin onset and sleep-wake behavior in young adults.","authors":"Taisuke Eto, Yuki Nishimura, Hiroki Ikeda, Taiki Kunieda, Minori Enomoto, Tomohide Kubo, Ana Adan, Shingo Kitamura","doi":"10.1080/07420528.2025.2532795","DOIUrl":"10.1080/07420528.2025.2532795","url":null,"abstract":"<p><p>The Reduced Morningness-Eveningness Questionnaire (rMEQ), a five-item version of the 19-item MEQ, is a practical tool for assessing circadian typology or \"morningness\" and \"eveningness\" preference. Although we previously validated the Japanese version of rMEQ with MEQ, external validation against an established standard was lacking. This study aimed to additionally validate the Japanese rMEQ in young adults by assessing the dim light melatonin onset (DLMO), a marker of circadian phase, and subjective/objective sleep-wake habits. Twenty healthy young adults (mean age: 23.0 ± 1.9 years) participated in this study and were assessed the circadian typology by rMEQ and MEQ. The sleep-wake habits were measured by sleep diary, actigraphy and the Pittsburgh Sleep Quality Index (PSQI). Salivary melatonin samples were collected to determine DLMO. Results showed significant correlations between rMEQ scores and DLMO, as well as sleep variables based on sleep diary, actigraphy and PSQI. Correlations between rMEQ scores and these variables were consistently stronger than those for MEQ. These findings highlight the validity of the Japanese rMEQ and that the rMEQ more accurately reflects circadian typology and internal circadian phases compared to MEQ, at least in Japanese young adults. The Japanese rMEQ could be a valuable tool for efficiently assessing circadian typologies in Japanese young adults.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-8"},"PeriodicalIF":2.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of natural orexin 2 receptor antagonists from Valeriana species: A potential approach for insomnia treatment.","authors":"Aparna G Shenoy, Vishal Ravi, Suhail Subair, Durairaj Ragu Varman, Rajesh Raju, Niyas Rehman","doi":"10.1080/07420528.2025.2529398","DOIUrl":"https://doi.org/10.1080/07420528.2025.2529398","url":null,"abstract":"<p><p>The orexinergic system, comprising orexin-A and orexin-B neuropeptides that bind to OX1R and OX2R receptors, plays a critical role in regulating sleep-wake cycles, appetite, and alertness. OX2R is particularly important for promoting arousal and non-rapid eye movement (NREM) sleep and has been linked to sleep disorders such as insomnia and narcolepsy. Although OX2R antagonists like suvorexant have shown therapeutic promise, they are often associated with side effects including cognitive impairment and dependence, highlighting the need for safer alternatives. This study employed an <i>in-silico</i> approach to identify natural OX2R antagonists from Valeriana species. Phytochemicals were screened based on molecular docking and favourable ADME/T profiles. Molecular dynamics (MD) simulations and post-MD analysis confirmed stable binding of hesperidine and valerosidate to OX2R. Principal component analysis (PCA) revealed minimal conformational variability while gibbs free energy landscape (FEL) analysis and MM-PBSA binding free energy calculations further supported the strong binding of hesperidine and valerosidate to OX2R, comparable to suvorexant. These findings support hesperidine and valerosidate as promising, naturally derived OX2R antagonists, and warrant further invitro and invivo investigations for potential therapeutic application in insomnia treatment.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-14"},"PeriodicalIF":2.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary on the relationship between circadian type and physical activity as predictors of cognitive performance during simulated nightshifts.","authors":"Efrem Kentiba","doi":"10.1080/07420528.2025.2531993","DOIUrl":"https://doi.org/10.1080/07420528.2025.2531993","url":null,"abstract":"","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1"},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between biological rhythm and breastfeeding success in lactating women.","authors":"Seda Can Nart, Mesude Duman","doi":"10.1080/07420528.2025.2526696","DOIUrl":"https://doi.org/10.1080/07420528.2025.2526696","url":null,"abstract":"<p><p>This study aims to investigate the relationship between biological rhythm and breastfeeding success among breastfeeding women. Carried out as a descriptive and cross-sectional study, the present study was completed with 204 breastfeeding women. Data collection instruments included a \"Personal Information Form,\" the \"Biological Rhythm Interview of Assessment Scale (BRIAS)\" scale, and the \"LATCH Breastfeeding Assessment Tool.\" The BRIAS activity, the sleep and the social habits subdimensions negatively predicted LATCH scores (<i>p</i> < 0.001). The study determined that as disruptions in biological rhythms increased, breastfeeding success decreased.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-8"},"PeriodicalIF":2.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of prolonged exposure to different lighting conditions on MPTP-induced motor dysfunction and cognitive performance in male rats.","authors":"Hamideh Rahmati, Seyedeh Masoumeh Seyedhosseini Tamijani, Hamed Ghazvini, Behzad Garmabi, Ali Siahposht-Khachaki, Saba Niknamfar, Raheleh Rafaiee","doi":"10.1080/07420528.2025.2529343","DOIUrl":"https://doi.org/10.1080/07420528.2025.2529343","url":null,"abstract":"<p><p>Circadian rhythm disruption has been increasingly implicated in neurodegenerative diseases, including Parkinson's disease (PD). While motor impairments in PD are well-established, the impact of chronic circadian misalignment on both motor and cognitive dysfunctions remains underexplored. This study investigates the effect of prolonged exposure to different lighting conditions on MPTP-induced motor and cognitive deficits in a rat model. A total of 100 adult male Wistar rats were divided into five groups with different light-dark cycle conditions: standard 12-h light/12-h dark (12 L:12D), constant light (24 L), constant darkness (24D), 20-h light/4-h dark (20 L:4D), and 4-h light/20-h dark (20D:4 L). After 60 d of exposure to these conditions, each group was further divided into Parkinsonian (MPTP-treated) and non-Parkinsonian (saline-treated) subgroups. MPTP hydrochloride (25 µg/kg) was administered intranasally to induce Parkinsonism. Behavioral assessments, including the beam balance test, open field test, Y-maze test, and shuttle box test, were conducted to evaluate motor coordination and cognitive function. Immunohistochemical analyses of α-synuclein (α-syn) accumulation in the substantia nigra (SNc) and tyrosine hydroxylase (TH) expression in the suprachiasmatic nucleus (SCN) were performed. Data were analyzed using t test, one-way ANOVA and Tukey's post hoc test. MPTP administration significantly impaired motor function, as evidenced by increased crossing time in the beam balance test (<i>p</i> < 0.001) and reduced locomotor activity in the open field test (<i>p</i> < 0.01). Prolonged exposure to different lighting conditions further exacerbated these deficits, particularly in the 24 L and 20 L:4D groups (<i>p</i> < 0.0001). Cognitive assessments revealed that MPTP-treated rats exhibited marked deficits in working memory (Y-maze, <i>p</i> < 0.05) and associative learning (shuttle box, <i>p</i> < 0.01), with the most severe impairments observed in groups subjected to extreme light-dark alterations. Immunohistochemical analyses demonstrated significantly increased α-syn accumulation in the SNc (<i>p</i> < 0.0001) and decreased TH expression in the SCN (<i>p</i> < 0.001), particularly in MPTP-treated rats under photoperiod disruption. Chronic photoperiod disruption exacerbates MPTP-induced motor dysfunction, cognitive impairment, and neurodegeneration. Targeting circadian regulation may offer a potential therapeutic approach for mitigating neurodegenerative progression in PD.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-16"},"PeriodicalIF":2.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscular strength and power and endurance performance at loads exceeding 25% of one-repetition maximum are unaffected by time-of-day in resistance-trained male participants.","authors":"Marta Del Val-Manzano, Juan Jesús Montalvo-Alonso, David Valadés, Carmen Ferragut, Julio Martín-López, Álvaro López-Samanes, Alberto Pérez-López","doi":"10.1080/07420528.2025.2524520","DOIUrl":"https://doi.org/10.1080/07420528.2025.2524520","url":null,"abstract":"<p><p>The aim of this study was to examine the influence of the time-of-day on muscular strength, power and endurance performance in resistance-trained individuals. Fourteen resistance-trained males (age: 26.3 ± 6.7 years) underwent a randomized, counterbalanced cross-over trial. After a familiarization session, participants underwent two trials performing in the morning (9:00 h) and in the evening (18:00 h) a muscular strength and power assessment for bench press and back squat exercises at 25%, 50%, 75%, 90%, and 100% of one-repetition maximum (1RM). Then, muscular endurance was assessed for both exercises at 65%1RM, performing one set until failure. Once completed, isometric strength and vertical jump capacity (CMJ) tests were also performed. Only back squat exercise at 25% 1RM reported higher performance in the evening compared to the morning at mean velocity and mean and peak power (11-13%, <i>p</i> = 0.018-0.031, g = 1.91-2.20). Also, CMJ power was higher in the evening compared to the morning trial (2.5%, <i>p</i> = 0.002, g = 0.23). No statistical differences were found in the remaining loads, exercises or tests. In conclusion, circadian rhythm affects muscular strength and power performance at low (≤25% 1RM) but not moderate-to-higher loads in resistance-trained male participants, an effect observed in lower-body (e.g. back squat and vertical jump) but not in upper-body exercises (e.g. bench press).</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-9"},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ang II-induced oscillation of clock genes can attenuate phenotypic transformation in vascular smooth muscle cells by activating the AT1R/PLC/Ca²⁺/PKC/p-CREB pathway.","authors":"Conglan Ji, Tao Ge, Nan Wang, Kai Guo, Jun Liu, Kui Yang","doi":"10.1080/07420528.2025.2523515","DOIUrl":"https://doi.org/10.1080/07420528.2025.2523515","url":null,"abstract":"<p><p>This study aimed to investigate the role of clock genes Per1/Per2 in angiotensin II (Ang II)-induced vascular smooth muscle cells (VSMCs) phenotypic transformation and the underlying mechanisms. Primary rat VSMCs were treated with Ang, valsartan or other inhibitors. Assays included PCR, Western blot (Per1, Per2, p-MLC₂₀, p-CREB, AT1R), cell viability (MTT, Ki67, binuclear count), cell cycle, calcium and IP3. 50% fetal bovine serum shock significantly reduced the proliferation-promoting effect of Ang. Ang significantly increased the expression of Per1/Per2 mRNA at ZT3 but decreased it at ZT19 and ZT23 correlating with p-MLC₂₀ changes. Valsartan (AT1R inhibitor), Calphostin C (PKC inhibitor), U73122 (PLC inhibitor), 2-APB (IP3R blocker) and dantrolene sodium salt (Calcium channel protein inhibitor) significantly blocked the effects of Ang on Per1/Per2 genes. Ang significantly increased the p-CREB expression, IP3 and [Ca²⁺]i concentration transiently but decreased it in the long term. However, Ang significantly decrease Per1, Per2, and AT1R proteins expression transiently but increased it in the long term. Finally, silencing Per1 and Per2 enhances Ang-induced proliferation of VSMCs. Ang II triggers Per1/Per2 oscillation via the AT1R/PLC/Ca²⁺/PKC/p-CREB axis. Remarkably, the resultant AT1R-Per1/Per2 feedback loop counteracts Ang II-driven VSMC phenotypic transformation.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-14"},"PeriodicalIF":2.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian gene polymorphisms and cancer: Insights into development and risk.","authors":"Danixa Llanos, Kevins Jara-Medina, Luis Lillo, Gerardo Cabello-Guzman, Constanza Lagunas, Francisco J Valenzuela-Melgarejo","doi":"10.1080/07420528.2025.2522816","DOIUrl":"https://doi.org/10.1080/07420528.2025.2522816","url":null,"abstract":"<p><p>The modern lifestyle imposes new stressors on our physiology, disrupting the natural adaptability and timekeeping abilities regulated by light exposure during dark hours. These chronodisruptors affect the timing of metabolic and hormonal processes, potentially interfering with homeostasis and cellular functions such as the cell cycle. Increasing evidence links such circadian disruption to an increased risk of diseases, notably cancer. Genetic variations can also influence this disturbance in circadian clock genes, which form the core components of the circadian rhythm regulatory system, including BMAL-1, CLOCK, PER 1-3, CRY 1-2, RORA, NPAS2, and TIMELESS. This review explores the potential of these polymorphisms in contributing to the timing disruptions in circadian rhythms, impacting cell cycle checkpoints like WEE1, Cyclins, and C-MYC. Further investigations into the genetic components and their relationship with cancer risk are critical for understanding their intricate connections with the circadian system and identifying novel molecular pathways that could be utilized for cancer diagnosis and prevention.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1-17"},"PeriodicalIF":2.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}