Clinical and laboratory haematology最新文献_第3页

The laboratory diagnosis of malaria. The Malaria Working Party of The General Haematology Task Force of the British Committee for Standards in Haematology. 疟疾的实验室诊断。英国血液学标准委员会一般血液学工作组的疟疾工作组。

Clinical and laboratory haematology Pub Date : 1997-09-01

引用次数: 0

An original method to study autoantibody specificity in haemoglobin stained eluates by the column agglutination techniques. 用柱凝集技术研究血红蛋白染色洗脱液中自身抗体特异性的一种新颖方法。

Clinical and laboratory haematology Pub Date : 1997-09-01

F Fiorin, M R Cozzi, P Pradella, A Steffan, R Potenza, V De Angelis

{"title":"An original method to study autoantibody specificity in haemoglobin stained eluates by the column agglutination techniques.","authors":"F Fiorin, M R Cozzi, P Pradella, A Steffan, R Potenza, V De Angelis","doi":"","DOIUrl":"","url":null,"abstract":"When studying autoantibody specificity by the indirect antiglobulin test with column agglutination techniques ether and xylene elution techniques result in haemoglobin stained eluates which give a red colouration to the gel or glass beads and do not allow the identification of positive reactions. Xylene eluates were incubated with commercially available group O-test red cell panels at 37 degrees C for 45 min in the wells of a microtitre plate in a 3:1 eluate:red cell ratio. After washing with normal saline, sensitized red cells, resuspended in low ionic strength solution (LISS), were applied onto the microtubes containing the antiglobulin serum and positive reactions were recorded after centrifugation. We studied the specificity of 35 autoantibody containing eluates from 12 patients with lymphoproliferative disorders (six having autoimmune haemolysis) and 23 HIV patients without autoimmune haemolysis. All patients had a gel or column positive (IgG) direct antiglobulin test while the tube direct antiglobulin test failed to show red cell bound IgG. We found a reactive indirect antiglobulin test in 20/23 eluates from HIV infected patients (with a panreactive specificity), in all patients with autoimmune haemolysis (one with anti-C, two with anti-E, one with anti-K and two with a panreactive specificity) and in all patients with positive direct antiglobulin test but without immune mediate haemolysis (in all cases with panreactive specificity). The method proposed is a promising tool for the study of the specificity of antibody containing haemoglobin stained eluates; in this study it allowed us to confirm that some HIV patients have specific binding of IgG on their RBC and to identify the specificity of tube test non-reactive eluates.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"19 3","pages":"209-11"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20283905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Priapism in a patient with protein C deficiency. 蛋白C缺乏症患者的阴茎勃起。

Clinical and laboratory haematology Pub Date : 1997-09-01

S Daryanani, J T Wilde

引用次数: 0

Comparison of oral anticoagulant control by a nurse-practitioner using a computer decision-support system with that by clinicians. 执业护士使用计算机决策支持系统与临床医生使用计算机决策支持系统进行口服抗凝血控制的比较。

Clinical and laboratory haematology Pub Date : 1997-09-01

B D Vadher, D L Patterson, M Leaning

{"title":"Comparison of oral anticoagulant control by a nurse-practitioner using a computer decision-support system with that by clinicians.","authors":"B D Vadher, D L Patterson, M Leaning","doi":"","DOIUrl":"","url":null,"abstract":"With increasing work-loads in anticoagulant clinics different methods of service delivery need evaluation. The quality of anticoagulant control achieved by a nurse-practitioner using a computer decision-support system (CDSS) was compared with that achieved by trainee doctors without CDSS. Eighty-one out-patients (group A, therapeutic range 2-3) and 96 out-patients (group B, therapeutic range 3-4.5) were randomized to management by a nurse-practitioner or by trainee doctors (clinicians). Thirty-seven patients in group A and 50 patients in group B were randomized to be managed by the nurse-practitioner. In group A, patients in the nurse-practitioner group spent a longer time in the therapeutic range than those in the clinician group (60.7% compared with 51.6%). Dose suggestion acceptance in the nurse-practitioner group (88%) was higher compared with agreement between the CDSS and the clinicians (60%). In group B, patients in the clinician group spent a slightly longer time in the therapeutic range (70% compared with 67.6%). Acceptance of dose suggestion was lower in the nurse-practitioner group (67%) compared with agreement between the CDSS and the clinicians (73%). In conclusion, the CDSS can improve the quality of control of warfarin therapy by a nurse-practitioner over that by trainee doctors for the therapeutic range 2-3. Similar quality of control is achieved for the therapeutic range 3-4.5. The CDSS may be used by nurse-practitioners to achieve safe and effective anticoagulation in hospital-based or out-reach anticoagulant clinics.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"19 3","pages":"203-7"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20283904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evaluation of the Spuncrit infra-red analyser for measurement of haematocrit. Spuncrit红外分析仪用于红细胞压积测量的评价。

Clinical and laboratory haematology Pub Date : 1997-09-01

M S Weatherall, K M Sherry

{"title":"An evaluation of the Spuncrit infra-red analyser for measurement of haematocrit.","authors":"M S Weatherall, K M Sherry","doi":"","DOIUrl":"","url":null,"abstract":"An open, single centre study was carried out to evaluate the accuracy of the Spuncrit (Micro Diagnostics, Bethlehem, PA, USA) infra-red analyser which can be used for near-patient testing to measure haematocrit and estimate haemoglobin concentration. The primary comparison was with the Sysmex NE1500 (Tao Medical) analyser situated in the main hospital laboratory. Secondary comparison was with the Ciba Corning 288 (Ciba Corning Diagnostics Ltd, Halstead, UK) blood gas analyser currently used for near-patient testing in the Northern General Hospital. A total of 217 samples from 50 patients was analysed. The Pearson's correlation coefficients for haematocrit and haemoglobin concentration between the Spuncrit and Sysmex NE1500 and between the Spuncrit and Ciba Corning 288 were all close, between 0.85 and 0.92. The method of Bland and Altman was used to assess agreement between the results of the Spuncrit and the Sysmex NE1500. The agreement for haematocrit was good with 2 SD of the Spuncrit results being between -5.66 and +4.42% of the measurement from the Sysmex NE1500. In conclusion, the Spuncrit haematocrit measurement agreed well with results from the central laboratory, but the estimated haemoglobin concentrations agreed less well and three reasons are discussed.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"19 3","pages":"183-6"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20282899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evaluation of the differential from the Abbott CD 3500 in a population of patients with haematological abnormalities. 评估雅培cd3500在血液学异常人群中的差异。

Clinical and laboratory haematology Pub Date : 1997-09-01

J Iles-Mann, J Henniker

{"title":"An evaluation of the differential from the Abbott CD 3500 in a population of patients with haematological abnormalities.","authors":"J Iles-Mann, J Henniker","doi":"","DOIUrl":"","url":null,"abstract":"The Cell Dyn 3500 (CD 3500) Haematology Analyser (Abbott Diagnostics, Lane Cove, NSW, Australia) has been evaluated to determine the reliability of the differential and white cell suspect flagging in a population of patients with known haematological abnormalities. The evaluation included the assessment of white cell differential parameters in addition to sensitivity, specificity and efficiency of white cell suspect flagging. The study showed that the CD 3500 is an efficient and sensitive screening tool for detecting the presence of clinically significant white cell abnormalities. Generally, the Blast and Band flags demonstrated the highest level of false positive flagging (lowest sensitivity). The immature granulocyte flag was found to be an extremely reliable indicator of the presence of myelocytes, metamyelocytes and promyelocytes on the film. There was a 0.4% false negative rate where significant numbers of variant lymphocytes (> 10%) were not detected by the CD 3500 on flagging alone. When analyser flags and white cell scatter distribution are considered in combination with defined laboratory limits, all white cell suspect flags have acceptable sensitivity, specificity and efficiency rates.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"19 3","pages":"191-6"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20283902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum levels of tumour necrosis factor-alpha predict response to recombinant human erythropoietin in patients with myelodysplastic syndrome. 血清肿瘤坏死因子- α水平预测重组人促红细胞生成素对骨髓增生异常综合征患者的反应。

Clinical and laboratory haematology Pub Date : 1997-09-01

R Stasi, M Brunetti, S Bussa, M Conforti, L S Martin, M La Presa, M Bianchi, A Parma, A Pagano

{"title":"Serum levels of tumour necrosis factor-alpha predict response to recombinant human erythropoietin in patients with myelodysplastic syndrome.","authors":"R Stasi, M Brunetti, S Bussa, M Conforti, L S Martin, M La Presa, M Bianchi, A Parma, A Pagano","doi":"","DOIUrl":"","url":null,"abstract":"We measured pretreatment serum levels of tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in 25 patients with myelodysplastic syndrome receiving recombinant human erythropoietin (rhEPO) at dosages up to 300 U/kg thrice weekly for 12 weeks. Both TNF-alpha and IL-1 beta levels were measured using commercially available enzyme-linked immunoassays. A complete response (CR) was defined as a rise in untransfused haemoglobin concentrations of at least 2 g/dl or a 100% decrease in RBC transfusion requirements over the treatment period; a partial response (PR) was an increase in untransfused haemoglobin values of 1-2 g/dl or a decrease in RBC transfusion requirements equal to or greater than 50%; no response (NR) was defined as a response less than a PR. After 12 weeks of rhEPO treatment, four patients showed a CR, five patients a PR, and 16 patients NR. Serum levels of both TNF-alpha (80.5 %/- 64.8 vs 8.1 +/- 4.2 ng/l, P < 0.001) and IL-1 beta (60.4 +/- 49.9 vs 8.9 +/- 4.7 ng/l, P < 0.001) were higher in MDS patients than in a group of 28 normal controls. Responders (CR + PR) showed significantly lower serum levels of TNF-alpha than non-responders (21.6 +/- 26.2 vs 106.3 +/- 60.8 ng/l, P < 0.001), whereas IL-1 beta concentrations between those who benefited from therapy and unresponsive cases were not significantly different (39.8 +/- 48.9 vs 73.4 +/- 48.2 ng/l, P = 0.120). It is noteworthy that TNF-alpha levels were within the normal range in all responsive patients but one, whereas all non-responders presented elevated cytokine concentrations. No relationship was found between TNF-alpha or IL-1 beta values and haemoglobin levels, transfusion requirement, serum EPO or ferritin concentrations. We conclude that pre-treatment TNF-alpha levels might help to select those MDS patients who are most likely to benefit from rhEPO treatment.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"19 3","pages":"197-201"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20283903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of cyclosporin A and etoposide. Clinical and in vitro assessment in blast phase of chronic myeloid leukaemia. 环孢素A与依托泊苷的相互作用。慢性髓性白血病胚期临床及体外评价。

Clinical and laboratory haematology Pub Date : 1997-09-01

R C Maia, H Noronha, F C Vasconcelos, V M Rumjanek

引用次数: 0

Sea-blue histiocytosis and pancytopaenia associated with chronic total parenteral nutrition administration. 海蓝色组织细胞增多症和全血细胞减少症与慢性全肠外营养管理有关。

Clinical and laboratory haematology Pub Date : 1997-09-01

D J Meiklejohn, H Baden, M Greaves

引用次数: 0

Serum levels of tumour necrosis factor-alpha predict response to recombinant human erythropoietin in patients with myelodysplastic syndrome. 血清肿瘤坏死因子- α水平预测重组人促红细胞生成素对骨髓增生异常综合征患者的反应。

Clinical and laboratory haematology Pub Date : 1997-09-01 DOI: 10.1046/J.1365-2257.1997.00064.X

R. Stasi, M. Brunetti, S. Bussa, M. Conforti, L. S. Martin, M. Presa, M. Bianchi, A. Parma, A. Pagano

{"title":"Serum levels of tumour necrosis factor-alpha predict response to recombinant human erythropoietin in patients with myelodysplastic syndrome.","authors":"R. Stasi, M. Brunetti, S. Bussa, M. Conforti, L. S. Martin, M. Presa, M. Bianchi, A. Parma, A. Pagano","doi":"10.1046/J.1365-2257.1997.00064.X","DOIUrl":"https://doi.org/10.1046/J.1365-2257.1997.00064.X","url":null,"abstract":"We measured pretreatment serum levels of tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in 25 patients with myelodysplastic syndrome receiving recombinant human erythropoietin (rhEPO) at dosages up to 300 U/kg thrice weekly for 12 weeks. Both TNF-alpha and IL-1 beta levels were measured using commercially available enzyme-linked immunoassays. A complete response (CR) was defined as a rise in untransfused haemoglobin concentrations of at least 2 g/dl or a 100% decrease in RBC transfusion requirements over the treatment period; a partial response (PR) was an increase in untransfused haemoglobin values of 1-2 g/dl or a decrease in RBC transfusion requirements equal to or greater than 50%; no response (NR) was defined as a response less than a PR. After 12 weeks of rhEPO treatment, four patients showed a CR, five patients a PR, and 16 patients NR. Serum levels of both TNF-alpha (80.5 %/- 64.8 vs 8.1 +/- 4.2 ng/l, P < 0.001) and IL-1 beta (60.4 +/- 49.9 vs 8.9 +/- 4.7 ng/l, P < 0.001) were higher in MDS patients than in a group of 28 normal controls. Responders (CR + PR) showed significantly lower serum levels of TNF-alpha than non-responders (21.6 +/- 26.2 vs 106.3 +/- 60.8 ng/l, P < 0.001), whereas IL-1 beta concentrations between those who benefited from therapy and unresponsive cases were not significantly different (39.8 +/- 48.9 vs 73.4 +/- 48.2 ng/l, P = 0.120). It is noteworthy that TNF-alpha levels were within the normal range in all responsive patients but one, whereas all non-responders presented elevated cytokine concentrations. No relationship was found between TNF-alpha or IL-1 beta values and haemoglobin levels, transfusion requirement, serum EPO or ferritin concentrations. We conclude that pre-treatment TNF-alpha levels might help to select those MDS patients who are most likely to benefit from rhEPO treatment.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"24 1","pages":"197-201"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84258263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21