Clinical and laboratory haematology最新文献_第10页

Letter from America: the end of civilization as we know it? 美国来信:我们所知的文明的终结?

Clinical and laboratory haematology Pub Date : 1995-09-01

S A Bentley

引用次数: 0

Low serum erythropoietin--a strong diagnostic criterion of primary polycythaemia even at normal haemoglobin levels. 低血清促红细胞生成素-原发性红细胞增多症的一个强有力的诊断标准，即使在正常血红蛋白水平。

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01239.X

M. Messinezy, N. Westwood, S. Woodcock, R. Strong, Thomas C. Pearson

{"title":"Low serum erythropoietin--a strong diagnostic criterion of primary polycythaemia even at normal haemoglobin levels.","authors":"M. Messinezy, N. Westwood, S. Woodcock, R. Strong, Thomas C. Pearson","doi":"10.1111/J.1365-2257.1995.TB01239.X","DOIUrl":"https://doi.org/10.1111/J.1365-2257.1995.TB01239.X","url":null,"abstract":"Estimations of serum erythropoietin level by enzyme immunoassay (EIA) kit were made in 42 patients with primary polycythaemia, and in a comparison group consisting of 41 patients with secondary polycythaemia and 47 patients with idiopathic erythrocytosis. The majority of patients with primary polycythaemia were undergoing treatment by venesection and therefore had Hb levels in the normal range at the time of erythropoietin estimation. In primary polycythaemia, 64% of the first samples taken from each patient were below the reference range for serum erythropoietin in normal individuals. When two samples were taken from each patient 72% had low values in one or both samples. In the comparison group, analysis of those patients who had two samples taken showed only one individual with secondary polycythaemia and none with idiopathic erythrocytosis who had a serum erythropoietin level below the reference range. The finding of low serum erythropoietin in patients with primary polycythaemia, even when Hb levels are normal due to venesection, is of high diagnostic specificity (few false positive results) and useful diagnostic sensitivity (28% false negative results). It is proposed that future diagnostic criteria of primary polycythaemia should include the finding of a serum erythropoietin level below the lower limit of normal in at least one of two serum samples taken on different occasions.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"39 1","pages":"217-20"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81218096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61

Acute non-lymphoblastic leukaemia with t(16;21): case report with a review of the literature. 急性非淋巴细胞白血病伴t(16;21): 1例报告并文献复习。

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01244.X

M. Hiyoshi, K. Koh, T. Yamane, N. Tatsumi

引用次数: 12

Letter from America: the end of civilization as we know it? 美国来信:我们所知的文明的终结?

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01246.X

S. Bentley

引用次数: 0

Recombinant thromboplastin inhibition assay for the detection of lupus anticoagulant. 重组凝血活酶抑制法检测狼疮抗凝血药物。

Clinical and laboratory haematology Pub Date : 1995-09-01

E Zanon, M A Saracino, P Simioni, L Scarano, B Girolami, A Girolami

{"title":"Recombinant thromboplastin inhibition assay for the detection of lupus anticoagulant.","authors":"E Zanon, M A Saracino, P Simioni, L Scarano, B Girolami, A Girolami","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Tissue thromboplastin inhibition assay (TTI) is a sensitive test for lupus anticoagulant (LA). We have performed TTI in 12 LA positive patients using a new recombinant human tissue factor (Innovin, IN) and compared it with Thromborel S (TH), a commonly used human placenta thromboplastin. The effect of using two different dilutions of each thromboplastin (1:10 & 1:26) was investigated. A 1:26 dilution discriminated better than the 1:10 and this was more evident for Innovin. The mean value obtained with a 1:26 IN dilution was statistically different from that observed with TH at the same dilution. Furthermore, when PT and TTI ratios were considered, differences were statistically significant and seemed to increase depending on thromboplastin dilutions. When we used IN at 1:26 all LA positive patients had a value > 1.2. Then we compared TTI at a 1:26 dilution with dilute Russell's Viper Venom Time (dRVVT) in 50 consecutive patients with suspected lupus anticoagulant not treated with warfarin or heparin. In these patients the diagnosis of lupus anticoagulant was carried out using dilute APTT mixing studies and a platelet neutralization procedure: four out of 50 patients thus tested were LA positive. When dRVVT or TTI-I 1:26 were used, five patients were positive for lupus anticoagulant. Innovin showed similar sensitivity of dRVVT for detection of lupus anticoagulant. It is likely that higher dilutions of thromboplastins could further improve the specificity of this method.</p>","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"17 3","pages":"231-5"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19694007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of monocyte counting with two automated instruments by the use of CD 14-specific immunomagnetic Dynabeads. 使用cd14特异性免疫磁头对两种自动仪器单核细胞计数的评价。

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01241.X

L. Theodorsen

{"title":"Evaluation of monocyte counting with two automated instruments by the use of CD 14-specific immunomagnetic Dynabeads.","authors":"L. Theodorsen","doi":"10.1111/J.1365-2257.1995.TB01241.X","DOIUrl":"https://doi.org/10.1111/J.1365-2257.1995.TB01241.X","url":null,"abstract":"Accurate determination of monocytes can be a problem with automated haematological analysers as well as with visual microscopy. We have compared monocyte counts from our two routine automated instruments, Technicon H*1 and Sysmex NE-1500. In the NE-1500 the monocyte counts appear to increase with increasing concentrations of neutrophils, compared with the H*1 results. The accuracy of the monocyte counts was assessed using Dynabeads M-450 CD14. These uniform magnetizable polystyrene beads coated with monoclonal antibody specific for CD14 membrane antigen isolated monocytes from the blood samples. The influence of this depletion on the monocyte counts and histograms from the two instruments and by visual microscopy were compared. On average 90% of the monocytes counted by visual microscopy were removed from six blood samples. In the same samples the beads removed an average of 75% of cells identified as monocytes by the H*1 but only 49% of those identified as monocytes by the NE-1500. These Dynabeads can be a useful tool in evaluating monocyte identification in different haematological situations and for manufacturers developing technology for automated leucocyte differential counting.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"332 1","pages":"225-9"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73146441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

An unusual case of hypersomnolence syndrome. 嗜睡症的罕见病例。

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/j.1365-2257.1995.tb01245.x

M. Gandhi, J. E. Chandler

引用次数: 0

Risk factors for arterial disease: laboratory assessment 动脉疾病的危险因素:实验室评估

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01252.X

G. Lowe

引用次数: 0

Recombinant thromboplastin inhibition assay for the detection of lupus anticoagulant. 重组凝血活酶抑制法检测狼疮抗凝血药物。

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/J.1365-2257.1995.TB01242.X

E. Zanon, M. A. Saracino, P. Simioni, L. Scarano, B. Girolami, A. Girolami

{"title":"Recombinant thromboplastin inhibition assay for the detection of lupus anticoagulant.","authors":"E. Zanon, M. A. Saracino, P. Simioni, L. Scarano, B. Girolami, A. Girolami","doi":"10.1111/J.1365-2257.1995.TB01242.X","DOIUrl":"https://doi.org/10.1111/J.1365-2257.1995.TB01242.X","url":null,"abstract":"Tissue thromboplastin inhibition assay (TTI) is a sensitive test for lupus anticoagulant (LA). We have performed TTI in 12 LA positive patients using a new recombinant human tissue factor (Innovin, IN) and compared it with Thromborel S (TH), a commonly used human placenta thromboplastin. The effect of using two different dilutions of each thromboplastin (1:10 & 1:26) was investigated. A 1:26 dilution discriminated better than the 1:10 and this was more evident for Innovin. The mean value obtained with a 1:26 IN dilution was statistically different from that observed with TH at the same dilution. Furthermore, when PT and TTI ratios were considered, differences were statistically significant and seemed to increase depending on thromboplastin dilutions. When we used IN at 1:26 all LA positive patients had a value > 1.2. Then we compared TTI at a 1:26 dilution with dilute Russell's Viper Venom Time (dRVVT) in 50 consecutive patients with suspected lupus anticoagulant not treated with warfarin or heparin. In these patients the diagnosis of lupus anticoagulant was carried out using dilute APTT mixing studies and a platelet neutralization procedure: four out of 50 patients thus tested were LA positive. When dRVVT or TTI-I 1:26 were used, five patients were positive for lupus anticoagulant. Innovin showed similar sensitivity of dRVVT for detection of lupus anticoagulant. It is likely that higher dilutions of thromboplastins could further improve the specificity of this method.","PeriodicalId":10285,"journal":{"name":"Clinical and laboratory haematology","volume":"38 1","pages":"231-5"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85550662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Molecular biology of blood groups: applications in prenatal blood grouping 血型分子生物学:在产前血型鉴定中的应用

Clinical and laboratory haematology Pub Date : 1995-09-01 DOI: 10.1111/j.1365-2257.1995.tb01255.x

D. Anstee

引用次数: 0