Circulatory shock最新文献_第8页

Time course of nitric oxide production and epithelial dysfunction during ischemia/reperfusion of the feline small intestine. 猫小肠缺血/再灌注过程中一氧化氮生成和上皮功能障碍的时间过程。

Circulatory shock Pub Date : 1994-03-01

S Kanwar, B L Tepperman, D Payne, L R Sutherland, P Kubes

引用次数: 0

Increased sinusoidal efflux of reduced and oxidized glutathione in rats with endotoxin/D-galactosamine hepatitis. 内毒素/ d -半乳糖胺肝炎大鼠还原性和氧化性谷胱甘肽的正弦外排增加。

Circulatory shock Pub Date : 1994-03-01

K Irita, H Okabe, A Koga, K Kurosawa, K Tagawa, M Yamakawa, J Yoshitake, S Takahashi

{"title":"Increased sinusoidal efflux of reduced and oxidized glutathione in rats with endotoxin/D-galactosamine hepatitis.","authors":"K Irita, H Okabe, A Koga, K Kurosawa, K Tagawa, M Yamakawa, J Yoshitake, S Takahashi","doi":"","DOIUrl":"","url":null,"abstract":"The changes in the concentrations of reduced (GSH) and oxidized glutathione (GSSG) in the plasma as well as in the liver were investigated in rats with endotoxin hepatitis. Hepatitis was induced by intraperitoneal co-administration of small doses of Escherichia coli endotoxin and D-galactosamine. In the liver, the concentration of GSH decreased and that of GSSG increased 12 hr later. In the plasma taken from the right atrium, the concentration of both GSH and GSSG increased. The GSH/GSSG ratio in the plasma decreased, as it did in the liver. The net sinusoidal efflux of GSH and GSSG from the liver was calculated by subtracting their concentrations in plasma of the infrahepatic, suprarenal inferior vena cava from those of the suprahepatic inferior vena cava. The efflux started to increase as early as 2-4 hr after the injection of the toxins. In contrast, a leakage of alanine aminotransferase, an elongation of prothrombin time, an inhibition of starvation ketosis, and an increase in serum concentration of total bilirubin were detected as late as 6-8 hr after the injection. We conclude that endotoxin/D-galactosamine hepatitis induced an increase in plasma concentrations of GSH as well as GSSG by increasing the efflux of these peptides from the liver, and that changes in plasma glutathione status might be useful and sensitive markers for liver damage.","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":"42 3","pages":"115-20"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19020123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo effect of methylprednisolone on lipopolysaccharide-induced superoxide production by pulmonary and circulating blood neutrophils in rats. 甲基强的松龙对大鼠肺和循环血液中性粒细胞脂多糖诱导的超氧化物产生的体内影响。

Circulatory shock Pub Date : 1994-03-01

C Tsuji, S Shioya

引用次数: 0

Elevations in circulating calcitonin gene-related peptide correlate with hemodynamic deterioration during endotoxic shock in pigs. 循环降钙素基因相关肽的升高与猪内毒素休克期间血流动力学恶化相关。

Circulatory shock Pub Date : 1994-03-01

W A Arden, R R Fiscus, X Wang, L Yang, R Maley, M Nielsen, S Lanzo, D R Gross

{"title":"Elevations in circulating calcitonin gene-related peptide correlate with hemodynamic deterioration during endotoxic shock in pigs.","authors":"W A Arden, R R Fiscus, X Wang, L Yang, R Maley, M Nielsen, S Lanzo, D R Gross","doi":"","DOIUrl":"","url":null,"abstract":"Calcitonin gene-related peptide (CGRP) is a potent vasodilatory neuropeptide, which may play a role in vascular dysfunction during septic shock. Sixteen pigs (25-50 kg) were anesthetized with ketamine and isoflurane in O2, and administered 100 micrograms/kg Escherichia coli lipopolysaccharide i.v. (LPS; n = 8) or saline vehicle (n = 8). Pigs were instrumented for hemodynamic determinations and blood sampling for CGRP assay (pg/ml) from the portal vein (PV) and the pulmonary (PA) and carotid (CA) arteries. Blood samples were collected into EDTA and aprotinin before (baseline) and at 60, 120, and 180 min after LPS administration. LPS caused significant deterioration in indices of hemodynamic function and a significant increase in plasma CGRP concentration at all sampling sites by 120 min (P < 0.01). No significant difference between sampling sites was recorded at any time. Plasma CGRP concentrations displayed significant negative correlations with mean arterial pressure, cardiac index, and left ventricular stroke work. These data confirm our previous findings of CGRP elevations in endotoxemic rats, and indicate that 1) LPS is a potent stimulus for the systemic release of CGRP, 2) increasing plasma CGRP concentrations temporally correlates with cardiovascular deterioration during LPS shock, and 3) there is little evidence that the portal circulation is a major source of circulating CGRP levels during LPS shock. Vasoactive neuropeptides, such as CGRP, may interact with other documented mediators of vascular dysfunction in the pathogenesis of septic shock.","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":"42 3","pages":"147-53"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19020128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo effect of methylprednisolone on lipopolysaccharide-induced superoxide production by pulmonary and circulating blood neutrophils in rats. 甲基强的松龙对大鼠肺和循环血液中性粒细胞脂多糖诱导的超氧化物产生的体内影响。

Circulatory shock Pub Date : 1994-03-01 DOI: 10.11501/3083092

C. Tsuji, S. Shioya

引用次数: 7

Protection of mice from mortality caused by living and heat-killed bacteria by SDZ MRL 953. SDZ mrl953对活菌和热杀菌致小鼠死亡的保护作用。

Circulatory shock Pub Date : 1994-03-01

E Schütze, J Hildebrandt, E Liehl, C Lam

{"title":"Protection of mice from mortality caused by living and heat-killed bacteria by SDZ MRL 953.","authors":"E Schütze, J Hildebrandt, E Liehl, C Lam","doi":"","DOIUrl":"","url":null,"abstract":"Protective effects of SDZ MRL 953, a monosaccharidic lipid A analog with a reduced toxicity, were investigated in models of experimental septic shock caused by injections of LPS, and inoculations of heat-killed or live bacteria. Female B6D2F1 mice were challenged with a combination of galactosamine (800 mg/kg) plus various doses of heat-killed isolates of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, and Staphylococcus aureus or LPS from Salmonella abortus equi. In some experiments, isolates of living bacteria at sublethal inocula were also combined with galactosamine. More than 90% of the animals died within 24 hr when the challenge was performed either simultaneously with or up to 4 hr after an intraperitoneal administration of galactosamine. No death was observed when galactosamine was omitted or administered after the microbial or LPS challenge. Pretreatment of the animals with SDZ MRL 953 (1-10 mg/kg) rendered the animals resistant to the lethal effects of both bacterial and LPS challenge in a time- and dose-dependent manner. The levels of TNF-alpha in control mice rose to greater than 600 pg/ml 2 hr postbacterial or LPS challenge, but were below detection in animals pretreated with SDZ MRL 953. Protection against both the infection and the toxicity of heat-killed bacteria or LPS was also achieved when murine anti-TNF-alpha monoclonal antibody was administered prophylactically. Together, these data suggest that SDZ MRL 953 enhances the resistance of mice against the toxicity of heat-killed gram-negative bacteria and S. aureus, and attenuates host responses to living bacteria which may lead to irreversible shock and death.","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":"42 3","pages":"121-7"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19020124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of vasopressin microvascular responses by endotoxin, endothelin, and nitric oxide. 内毒素、内皮素和一氧化氮对血管加压素微血管反应的影响。

Circulatory shock Pub Date : 1994-02-01

C H Baker, E T Sutton

{"title":"Modification of vasopressin microvascular responses by endotoxin, endothelin, and nitric oxide.","authors":"C H Baker, E T Sutton","doi":"","DOIUrl":"","url":null,"abstract":"The sensitivity of rat cremaster muscle arterioles to topically applied arginine vasopressin (AVP) is greatly increased by endotoxin (ENDT) [1]. The hypothesis is that the increase in vasoconstrictor sensitivity is in part due to modification of the AVP responses by endothelial compounds such as nitric oxide (NO) and endothelin. Reactivity of left cremaster muscle microvessels of pentobarbital anesthetized Sprague-Dawley rats was measured using videomicroscopy. Femoral arterial pressure as well as second and third order arteriolar (A2 and A3) vasoconstrictor threshold responses were determined for topical AVP (10(-15)-10(-6) M). These measurements were repeated in the presence of ENDT (6 mg/kg) alone and in the presence of the NO synthase inhibitor L-NAME (N omega-nitro-L-arginine methyl ester; 1 mg/kg) and ENDT (group 1). The control threshold (M)(-log) for arteriolar constriction by AVP was 9.4 +/- 0.7. After ENDT the threshold decreased significantly (P < 0.05) to 13.8 +/- 0.5, but returned to 9.0 +/- 0.5 after i.v. injected L-NAME. Acetylcholine (ACh) injected i.a. during AVP constriction significantly increased diameters at control and after ENDT, but not after L-NAME. In group 2 the AVP threshold was determined at control, after L-NAME plus hydroquinone (HQ), and at 30, 90, and 120 min post-ENDT in the presence of L-NAME + HQ. The AVP threshold at control was 9.0 +/- 0.3, after L-NAME 9.0 +/- 0.6, and after HQ 8.0 +/- 0.7. After L-NAME + HQ, the threshold was significantly increased to 7.3 +/- 0.2. After ENDT, in the presence of both antagonists, the threshold remained elevated at 7.4 +/- 0.2.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":"42 2","pages":"59-67"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18523599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of glucocorticoid receptor (GR) blockade on endotoxemia in rats. 糖皮质激素受体阻断对大鼠内毒素血症的影响。

Circulatory shock Pub Date : 1994-02-01

J Fan, X Q Gong, J Wu, Y F Zhang, R B Xu

引用次数: 0

Hydrazine sulfate selectively modulates the TNF response to endotoxin in mouse macrophages. 硫酸肼选择性调节小鼠巨噬细胞对内毒素的TNF反应。

Circulatory shock Pub Date : 1994-02-01

F Jia, D C Morrison, R Silverstein

引用次数: 0

Efficacy of a recombinant endotoxin neutralizing protein in rabbits with Escherichia coli sepsis. 重组内毒素中和蛋白对家兔大肠杆菌败血症的治疗效果。

Circulatory shock Pub Date : 1994-02-01

R Saladino, C Garcia, C Thompson, B Hammer, J Parsonnet, T Novitsky, G Siber, G Fleisher

{"title":"Efficacy of a recombinant endotoxin neutralizing protein in rabbits with Escherichia coli sepsis.","authors":"R Saladino, C Garcia, C Thompson, B Hammer, J Parsonnet, T Novitsky, G Siber, G Fleisher","doi":"","DOIUrl":"","url":null,"abstract":"Gram-negative bacterial sepsis is associated with endotoxemia and a high mortality rate. In previous studies, we demonstrated the therapeutic benefit of an anti-lipopolysaccharide factor isolated from amebocytes of Limulus polyphemus, and of a recombinant version of this protein, termed endotoxin neutralizing protein (ENP), in rabbits challenged with purified lipopolysaccharides. To assess the benefit of ENP in treating a live bacterial infection, we established a rabbit model of Escherichia coli (E. coli) peritonitis and bacteremia with high mortality despite gentamicin treatment. Twenty-four pairs of New Zealand white rabbits were challenged intraperitoneally (IP) with E. coli O18ac K1 in 5% porcine mucin (mean bacteria per dose = 2.5 x 10(8)). The animals were treated with intravenous (i.v.) gentamicin (2.5 mg/kg), and with either ENP (5 mg/kg) or saline i.v. at 1 hr after E. coli challenge. All rabbits were bacteremic 1 hr after challenge (geometric mean 4.1 +/- 1.2 x 10(4) cfu/mL). Peak geometric mean serum endotoxin (2.62 v 10.54 EU/mL, P = .013) and tumor necrosis factor (TNF) (2540 v 6438 TNF units/mL, P = .046) concentrations were lower in ENP-treated animals as compared to control animals. Seven of 24 animals treated with ENP survived 24 hr compared with 4 of 24 controls (Kaplan-Meier analysis, P = .19). However, in the subgroup of 13 paired animals in whom bacteremia was eliminated by gentamicin treatment, 5 of 13 ENP-treated animals survived 24 hr, compared with 1 of 13 controls (Kaplan-Meier analysis, P = .032).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":10280,"journal":{"name":"Circulatory shock","volume":"42 2","pages":"104-10"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19007559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0