Increase in the plasma concentration of reduced glutathione observed in rats with liver damage induced by lipopolysaccharide/D-galactosamine: effects of ulinastatin, a urinary trypsin inhibitor.

Circulatory shock Pub Date : 1993-12-01
H Okabe, K Irita, K Kurosawa, K Tagawa, A Koga, M Yamakawa, J Yoshitake, S Takahashi
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Abstract

The changes in plasma concentrations of reduced glutathione were investigated in rats with endotoxin hepatitis. An increase in serum alanine aminotransferase activity and in serum total bilirubin concentration was observed 12 hr after the intraperitoneal co-administration of small doses of Escherichia coli lipopolysaccharide and D-galactosamine in starved rats. At the same time, an increase in the plasma concentration of reduced glutathione was also observed. The increase in reduced glutathione from 14 +/- 2 to 20 +/- 9 microM (n = 11, P < 0.05) correlated well with that in serum alanine aminotransferase activity. Ulinastatin, a potent inhibitor of polymorphonuclear leukocyte elastase, partially counteracted all of these changes. Ulinastatin also reduced histological liver damage induced by endotoxin. We conclude that the increase in the plasma concentration of reduced glutathione reflects hepatocellular damage associated with endotoxin hepatitis. The partial reversal of the damage by ulinastatin is consistent with the proposal that the activation of polymorphonuclear leukocytes is involved in endotoxin hepatitis.

脂多糖/ d -半乳糖胺所致肝损伤大鼠血浆还原性谷胱甘肽浓度升高:尿胰蛋白酶抑制剂乌司他丁的作用
研究了内毒素肝炎大鼠血浆还原性谷胱甘肽浓度的变化。在饥饿大鼠腹腔注射小剂量大肠杆菌脂多糖和d -半乳糖胺12小时后,观察到血清丙氨酸转氨酶活性和血清总胆红素浓度升高。同时,还观察到血浆还原性谷胱甘肽浓度升高。还原型谷胱甘肽从14 +/- 2 μ m增加到20 +/- 9 μ m (n = 11, P < 0.05),与血清丙氨酸转氨酶活性的增加密切相关。乌司他丁,一种有效的多形核白细胞弹性酶抑制剂,部分抵消了所有这些变化。乌司他丁还能减轻内毒素引起的肝组织损伤。我们得出结论,血浆还原型谷胱甘肽浓度的增加反映了内毒素肝炎相关的肝细胞损伤。乌司他丁对损伤的部分逆转与多形核白细胞的激活参与内毒素肝炎的建议是一致的。
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