Chromosoma最新文献_第3页

Cross-species chromosome painting and repetitive DNA mapping illuminate the karyotype evolution in true crocodiles (Crocodylidae). 跨物种染色体绘制和重复DNA图谱揭示了真鳄鱼（鳄科）的核型进化。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-07-26 DOI: 10.1007/s00412-023-00806-6

Vanessa Sales-Oliveira, Marie Altmanová, Václav Gvoždík, Rafael Kretschmer, Tariq Ezaz, Thomas Liehr, Niklas Padutsch, Gabriel Badjedjea, Ricardo Utsunomia, Alongklod Tanomtong, Marcelo Cioffi

{"title":"Cross-species chromosome painting and repetitive DNA mapping illuminate the karyotype evolution in true crocodiles (Crocodylidae).","authors":"Vanessa Sales-Oliveira, Marie Altmanová, Václav Gvoždík, Rafael Kretschmer, Tariq Ezaz, Thomas Liehr, Niklas Padutsch, Gabriel Badjedjea, Ricardo Utsunomia, Alongklod Tanomtong, Marcelo Cioffi","doi":"10.1007/s00412-023-00806-6","DOIUrl":"10.1007/s00412-023-00806-6","url":null,"abstract":"Crocodilians have maintained very similar karyotype structures and diploid chromosome numbers for around 100 million years, with only minor variations in collinearity. Why this karyotype structure has largely stayed unaltered for so long is unclear. In this study, we analyzed the karyotypes of six species belonging to the genera Crocodylus and Osteolaemus (Crocodylidae, true crocodiles), among which the Congolian endemic O. osborni was included and investigated. We utilized various techniques (differential staining, fluorescence in situ hybridization with repetitive DNA and rDNA probes, whole chromosome painting, and comparative genomic hybridization) to better understand how crocodile chromosomes evolved. We studied representatives of three of the four main diploid chromosome numbers found in crocodiles (2n = 30/32/38). Our data provided new information about the species studied, including the identification of four major chromosomal rearrangements that occurred during the karyotype diversification process in crocodiles. These changes led to the current diploid chromosome numbers of 2n = 30 (fusion) and 2n = 38 (fissions), derived from the ancestral state of 2n = 32. The conserved cytogenetic tendency in crocodilians, where extant species keep near-ancestral state, contrasts with the more dynamic karyotype evolution seen in other major reptile groups.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":" ","pages":"289-303"},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos. H3K9和H4K20甲基转移酶直接参与了雄性柠檬扁球菌胚胎中父系染色体的异染色化。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-09-12 DOI: 10.1007/s00412-023-00809-3

Yakov A Osipov, Olga V Posukh, Darya A Kalashnikova, Polina A Antoshina, Petr P Laktionov, Polina A Skrypnik, Stepan N Belyakin, Prim B Singh

引用次数: 0

Nucleolin is required for multiple centrosome-associated functions in early vertebrate mitosis. 在脊椎动物早期有丝分裂过程中，需要 Nucleolin 发挥与中心体相关的多种功能。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-08-24 DOI: 10.1007/s00412-023-00808-4

Chandan Kumar, Sivaram V S Mylavarapu

引用次数: 0

Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting. 水蛙复合体的核型:跨种染色体绘制的结果。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-11-25 DOI: 10.1007/s00412-023-00812-8

Dmitrij Dedukh, Antonina Maslova, Ahmed Al-Rikabi, Niklas Padutsch, Thomas Liehr, Alla Krasikova

{"title":"Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting.","authors":"Dmitrij Dedukh, Antonina Maslova, Ahmed Al-Rikabi, Niklas Padutsch, Thomas Liehr, Alla Krasikova","doi":"10.1007/s00412-023-00812-8","DOIUrl":"10.1007/s00412-023-00812-8","url":null,"abstract":"Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":" ","pages":"329-342"},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138433514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulation of Tfh cell differentiation by β-hydroxybutyrylation modification of transcription factor Bcl6. 转录因子 Bcl6 的β-羟基丁酰化修饰对 Tfh 细胞分化的调控。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-05-25 DOI: 10.1007/s00412-023-00799-2

Jingtian Guo, Yimeng Wang, Lei Tang, Tiejun Tang, Zhuolan Li, Mengyuan Li, Liming Wang, Aizhong Zeng, Yuxiao Ma, Shihao Huang, Xiaomeng Jiang, Wei Guo

{"title":"The regulation of Tfh cell differentiation by β-hydroxybutyrylation modification of transcription factor Bcl6.","authors":"Jingtian Guo, Yimeng Wang, Lei Tang, Tiejun Tang, Zhuolan Li, Mengyuan Li, Liming Wang, Aizhong Zeng, Yuxiao Ma, Shihao Huang, Xiaomeng Jiang, Wei Guo","doi":"10.1007/s00412-023-00799-2","DOIUrl":"10.1007/s00412-023-00799-2","url":null,"abstract":"Transcriptional repressor B cell lymphoma 6 (Bcl6) is a major transcription factor involved in Tfh cell differentiation and germinal center response, which is regulated by a variety of biological processes. However, the functional impact of post-translational modifications, particularly lysine β-hydroxybutyrylation (Kbhb), on Bcl6 remains elusive. In this study, we revealed that Bcl6 is modified by Kbhb to affect Tfh cell differentiation, resulting in the decrease of cell population and cytokine IL-21. Furthermore, the modification sites are identified from enzymatic reactions to be lysine residues at positions 376, 377, and 379 by mass spectrometry, which is confirmed by site-directed mutagenesis and functional analyses. Collectively, our present study provides evidence on the Kbhb modification of Bcl6 and also generates new insights into the regulation of Tfh cell differentiation, which is a starting point for a thorough understanding of the functional involvement of Kbhb modification in the differentiations of Tfh and other T cells.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":" ","pages":"257-268"},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10209948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9895388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling of covalent modifications of histones to estimate the binding affinity. 建立组蛋白共价修饰模型，估算结合亲和力。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-05-20 DOI: 10.1007/s00412-023-00798-3

Ali Aslhashemi, Mahdi Rezaei Karamati, Hossein Motavalli, Milad Bastami

{"title":"Modeling of covalent modifications of histones to estimate the binding affinity.","authors":"Ali Aslhashemi, Mahdi Rezaei Karamati, Hossein Motavalli, Milad Bastami","doi":"10.1007/s00412-023-00798-3","DOIUrl":"10.1007/s00412-023-00798-3","url":null,"abstract":"Covalent histone modifications such as methylation, acetylation, phosphorylation, and other epigenetic modifications of the chromatin play an essential role in regulating eukaryotic cells of which most of these reactions are catalyzed by the enzymes. The binding energy of enzymes is often determined by experimental data via mathematical and statistical models due to specific modifications. Many theoretical models have been introduced to study histone modifications and reprogramming experiments in mammalian cells, in which all efforts in determining the affinity binding are essential part of the work. Here, we introduce a one-dimensional statistical Potts model to accurately determine the enzyme's binding free energy using the experimental data for various types of cells. We study the methylation of lysine 4 and 27 on histone H3 and suppose that each histone has one modification site with one of the seven states: H3K27me3, H3K27me2, H3K27me1, unmodified, H3K4me1, H3K4me2, and H3K4me3. Based on this model, the histone covalent modification is described. Moreover, by using simulation data, the histone's binding free energy and the energy of chromatin states are determined, when they are subject to changes from unmodified to active or repressive states, by finding the probability of the transition.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":" ","pages":"247-256"},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9495996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased genome size is caused by heterochromatin addition in two non-related bat species, Hesperoptenus doriae and Philetor brachypterus (Vespertilionidae, Chiroptera, Mammalia). 两种非亲缘关系的蝙蝠--Hesperoptenus doriae和Philetor brachypterus（蝙蝠科，翼手目，哺乳纲）--的异染色质增加导致基因组体积增大。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-06-16 DOI: 10.1007/s00412-023-00802-w

Marianne Volleth, Johann Greilhuber, Klaus-Gerhard Heller, Stefan Müller, Hoi-Sen Yong, Josef Loidl

{"title":"Increased genome size is caused by heterochromatin addition in two non-related bat species, Hesperoptenus doriae and Philetor brachypterus (Vespertilionidae, Chiroptera, Mammalia).","authors":"Marianne Volleth, Johann Greilhuber, Klaus-Gerhard Heller, Stefan Müller, Hoi-Sen Yong, Josef Loidl","doi":"10.1007/s00412-023-00802-w","DOIUrl":"10.1007/s00412-023-00802-w","url":null,"abstract":"The average genome size (GS) of bats, which are the only mammals capable of powered flight, is approximately 18% smaller than that of closely related mammalian orders. The low nuclear DNA content of Chiroptera is comparable to that of birds, which are also characterized by a high metabolic rate. Only a few chiropteran taxa possess notable amounts of constitutive heterochromatin. Here, we studied the karyotypes of two non-related vesper bat species with unusually high amounts of constitutive heterochromatin: Hesperoptenus doriae and Philetor brachypterus. Conventional staining methods and whole-chromosome painting with probes derived from Myotis myotis (2n = 44), showing a karyotype close to that of the presumed ancestor of Vespertilionidae, revealed Robertsonian fusions as the main type of rearrangement leading to the exceptionally reduced diploid chromosome number of 2n = 26 in both species. Moreover, both karyotypes are characterized by large blocks of pericentromeric heterochromatin composed of CMA-positive and DA-DAPI-positive segments. In H. doriae, the heterochromatin accumulation has resulted in a genome size of 3.22 pg (1C), which is 40% greater than the mean genome size for the family. For P. brachypterus, a genome size of 2.94 pg was determined, representing an increase of about 28%. Most notably, in H. doriae, the presence of additional constitutive heterochromatin correlates with an extended mitotic cell cycle duration in vitro. A reduction in diploid chromosome number to 30 or lower is discussed as a possible cause of the accumulation of pericentromeric heterochromatin in Vespertilionidae.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":" ","pages":"269-288"},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10012073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Casting histone variants during mammalian reproduction. 在哺乳动物繁殖过程中铸造组蛋白变异。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-09-01 DOI: 10.1007/s00412-023-00803-9

Germaine Karam, Antoine Molaro

引用次数: 0

Using Synthetic DNA Libraries to Investigate Chromatin and Gene Regulation. 利用合成DNA文库研究染色质和基因调控。

IF 1.6 4区生物学

Chromosoma Pub Date : 2023-09-01 Epub Date: 2023-05-15 DOI: 10.1007/s00412-023-00796-5

Holly Kleinschmidt, Cheng Xu, Lu Bai

引用次数: 0

New voices in epigenetics. 表观遗传学的新声音。