Chromosoma最新文献_第10页

A role of the Trx-G complex in Cid/CENP-A deposition at Drosophila melanogaster centromeres. Trx-G复合物在果蝇着丝粒中Cid/CENP-A沉积中的作用。

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-12-01 Epub Date: 2019-06-16 DOI: 10.1007/s00412-019-00711-x

Lucia Piacentini, Marcella Marchetti, Elisabetta Bucciarelli, Assunta Maria Casale, Ugo Cappucci, Paolo Bonifazi, Fioranna Renda, Laura Fanti

{"title":"A role of the Trx-G complex in Cid/CENP-A deposition at Drosophila melanogaster centromeres.","authors":"Lucia Piacentini, Marcella Marchetti, Elisabetta Bucciarelli, Assunta Maria Casale, Ugo Cappucci, Paolo Bonifazi, Fioranna Renda, Laura Fanti","doi":"10.1007/s00412-019-00711-x","DOIUrl":"https://doi.org/10.1007/s00412-019-00711-x","url":null,"abstract":"Centromeres are epigenetically determined chromatin structures that specify the assembly site of the kinetochore, the multiprotein machinery that binds microtubules and mediates chromosome segregation during mitosis and meiosis. The centromeric protein A (CENP-A) and its Drosophila orthologue centromere identifier (Cid) are H3 histone variants that replace the canonical H3 histone in centromeric nucleosomes of eukaryotes. CENP-A/Cid is required for recruitment of other centromere and kinetochore proteins and its deficiency disrupts chromosome segregation. Despite the many components that are known to cooperate in centromere function, the complete network of factors involved in CENP-A recruitment remains to be defined. In Drosophila, the Trx-G proteins localize along the heterochromatin with specific patterns and some of them localize to the centromeres of all chromosomes. Here, we show that the Trx, Ash1, and CBP proteins are required for the correct chromosome segregation and that Ash1 and CBP mediate for Cid/CENP-A recruitment at centromeres through post-translational histone modifications. We found that centromeric H3 histone is consistently acetylated in K27 by CBP and that nej and ash1 silencing respectively causes a decrease in H3K27 acetylation and H3K4 methylation along with an impairment of Cid loading.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"128 4","pages":"503-520"},"PeriodicalIF":1.6,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-019-00711-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37332478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Acknowledgement to reviewers 审稿人致谢

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-12-01 DOI: 10.1007/BF00292793

G. Casoni, S. Cavaliere, S. K. Chhabra, P. Chhajed, T. Çiftçi, A. Aggarwal, K. Amjadi, J. Annema, S. Bilaçeroğlu, Vincent Cottin

引用次数: 0

Genetic and epigenetic effects on centromere establishment 着丝粒建立的遗传和表观遗传效应

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-11-28 DOI: 10.1007/s00412-019-00727-3

Y. Ling, Zhongyang Lin, K. Yuen

引用次数: 5

Acknowledgement to reviewers 审稿人致谢

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-11-01 DOI: 10.1007/BF00352300

Editorial Office

引用次数: 0

The novel function of the Ph1 gene to differentiate homologs from homoeologs evolved in Triticum turgidum ssp. dicoccoides via a dramatic meiosis-specific increase in the expression of the 5B copy of the C-Ph1 gene 肥大小麦Ph1基因分化同源物的新功能。通过减数分裂特异性地增加C-Ph1基因的5B拷贝的表达

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-09-07 DOI: 10.1007/s00412-019-00724-6

Kanwardeep S. Rawale, M. A. Khan, K. Gill

引用次数: 6

SIRT7 promotes chromosome synapsis during prophase I of female meiosis. SIRT7在雌性减数分裂前期促进染色体突触。

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-09-01 Epub Date: 2019-06-29 DOI: 10.1007/s00412-019-00713-9

Berta N Vazquez, Cecilia S Blengini, Yurdiana Hernandez, Lourdes Serrano, Karen Schindler

{"title":"SIRT7 promotes chromosome synapsis during prophase I of female meiosis.","authors":"Berta N Vazquez, Cecilia S Blengini, Yurdiana Hernandez, Lourdes Serrano, Karen Schindler","doi":"10.1007/s00412-019-00713-9","DOIUrl":"https://doi.org/10.1007/s00412-019-00713-9","url":null,"abstract":"Sirtuins are NAD+-dependent protein deacylases and ADP-ribosyltransferases that are involved in a wide range of cellular processes including genome homeostasis and metabolism. Sirtuins are expressed in human and mouse oocytes yet their role during female gamete development are not fully understood. Here, we investigated the role of a mammalian sirtuin member, SIRT7, in oocytes using a mouse knockout (KO) model. Sirt7 KO females have compromised fecundity characterized by a rapid fertility decline with age, suggesting the existence of a diminished oocyte pool. Accordingly, Sirt7 KO females produced fewer oocytes and ovulated fewer eggs. Because of the documented role of SIRT7 in DNA repair, we investigated whether SIRT7 regulates prophase I when meiotic recombination occurs. Sirt7 KO pachynema-like staged oocytes had approximately twofold increased γH2AX signals associated with regions with unsynapsed chromosomes. Consistent with the presence of asynaptic chromosome regions, Sirt7 KO oocytes had fewer MLH1 foci (~one less), a mark of crossover-mediated repair, than WT oocytes. Moreover, this reduced level of crossing over is consistent with an observed twofold increased incidence of aneuploidy in Metaphase II eggs. In addition, we found that acetylated lysine 18 of histone H3 (H3K18ac), an established SIRT7 substrate, was increased at asynaptic chromosome regions suggesting a functional relationship between this epigenetic mark and chromosome synapsis. Taken together, our findings demonstrate a pivotal role for SIRT7 in oocyte meiosis by promoting chromosome synapsis and have unveiled the importance of SIRT7 as novel regulator of the reproductive lifespan.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"128 3","pages":"369-383"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-019-00713-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37116800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Special issue on “recent advances in meiosis from DNA replication to chromosome segregation” “从DNA复制到染色体分离的减数分裂新进展”特刊

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-09-01 DOI: 10.1007/s00412-019-00726-4

F. Cole, V. Borde

引用次数: 5

Crossing and zipping: molecular duties of the ZMM proteins in meiosis. 交叉和压缩:ZMM蛋白在减数分裂中的分子功能。

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-09-01 Epub Date: 2019-06-25 DOI: 10.1007/s00412-019-00714-8

Alexandra Pyatnitskaya, Valérie Borde, Arnaud De Muyt

{"title":"Crossing and zipping: molecular duties of the ZMM proteins in meiosis.","authors":"Alexandra Pyatnitskaya, Valérie Borde, Arnaud De Muyt","doi":"10.1007/s00412-019-00714-8","DOIUrl":"https://doi.org/10.1007/s00412-019-00714-8","url":null,"abstract":"Accurate segregation of homologous chromosomes during meiosis depends on the ability of meiotic cells to promote reciprocal exchanges between parental DNA strands, known as crossovers (COs). For most organisms, including budding yeast and other fungi, mammals, nematodes, and plants, the major CO pathway depends on ZMM proteins, a set of molecular actors specifically devoted to recognize and stabilize CO-specific DNA intermediates that are formed during homologous recombination. The progressive implementation of ZMM-dependent COs takes place within the context of the synaptonemal complex (SC), a proteinaceous structure that polymerizes between homologs and participates in close homolog juxtaposition during prophase I of meiosis. While SC polymerization starts from ZMM-bound sites and ZMM proteins are required for SC polymerization in budding yeast and the fungus Sordaria, other organisms differ in their requirement for ZMM in SC elongation. This review provides an overview of ZMM functions and discusses their collaborative tasks for CO formation and SC assembly, based on recent findings and on a comparison of different model organisms.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"128 3","pages":"181-198"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-019-00714-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37362871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 86

Maternal obesity enhances oocyte chromosome abnormalities associated with aging. 母亲肥胖增加与衰老相关的卵母细胞染色体异常。

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-09-01 Epub Date: 2019-07-08 DOI: 10.1007/s00412-019-00716-6

Yan Yun, Zijie Wei, Neil Hunter

{"title":"Maternal obesity enhances oocyte chromosome abnormalities associated with aging.","authors":"Yan Yun, Zijie Wei, Neil Hunter","doi":"10.1007/s00412-019-00716-6","DOIUrl":"https://doi.org/10.1007/s00412-019-00716-6","url":null,"abstract":"Obesity is increasing globally, and maternal obesity has adverse effects on pregnancy outcomes and the long-term health of offspring. Maternal obesity has been associated with pregnancy failure through impaired oogenesis and embryogenesis. However, whether maternal obesity causes chromosome abnormalities in oocytes has remained unclear. Here we show that chromosome abnormalities are increased in the oocytes of obese mice fed a high-fat diet and identify weakened sister-chromatid cohesion as the likely cause. Numbers of full-grown follicles retrieved from obese mice were the same as controls and the efficiency of in vitro oocyte maturation remained high. However, chromosome abnormalities presenting in both metaphase-I and metaphase-II were elevated, most prominently the premature separation of sister chromatids. Weakened sister-chromatid cohesion in oocytes from obese mice was manifested both as the terminalization of chiasmata in metaphase-I and as increased separation of sister centromeres in metaphase II. Obesity-associated abnormalities were elevated in older mice implying that maternal obesity exacerbates the deterioration of cohesion seen with advancing age.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"128 3","pages":"413-421"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-019-00716-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37405182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Ubiquitin-specific protease 26 (USP26) is not essential for mouse gametogenesis and fertility. 泛素特异性蛋白酶26 (USP26)在小鼠配子发生和生育中不是必需的。

IF 1.6 4区生物学

Chromosoma Pub Date : 2019-09-01 Epub Date: 2019-03-18 DOI: 10.1007/s00412-019-00697-6

Natalia Felipe-Medina, Laura Gómez-H, Yazmine B Condezo, Manuel Sanchez-Martín, José Luis Barbero, Isabel Ramos, Elena Llano, Alberto M Pendás

{"title":"Ubiquitin-specific protease 26 (USP26) is not essential for mouse gametogenesis and fertility.","authors":"Natalia Felipe-Medina, Laura Gómez-H, Yazmine B Condezo, Manuel Sanchez-Martín, José Luis Barbero, Isabel Ramos, Elena Llano, Alberto M Pendás","doi":"10.1007/s00412-019-00697-6","DOIUrl":"https://doi.org/10.1007/s00412-019-00697-6","url":null,"abstract":"Ubiquitin-specific protease 26 (USP26) is a deubiquitylating enzyme belonging to the USPs family with a transcription pattern restricted to the male germline. Since protein ubiquitination is an essential regulatory mechanism during meiosis, many efforts have been focused on elucidating the function of USP26 and its relationship with fertility. During the last decade, several studies have reported the presence of different polymorphisms in USP26 in patients with non-obstructive azoospermia (NOA) or severe oligozoospermia suggesting that this gene may be associated with human infertility. However, other studies have revealed the presence of these and novel polymorphisms, including nonsense mutations, in men with normal spermatogenesis as well. Thus, the results remain controversial and its function is unknown. In the present study, we describe the in vivo functional analysis of mice lacking USP26. The phenotypic analysis of two different Usp26-null mutants showed no overt-phenotype with both males and females being fertile. Cytological analysis of spermatocytes showed no defects in synapsis, chromosome dynamics, DNA repair, or recombination. Histopathological analysis revealed a normal distribution and number of the different cell types in both male and female mice. Finally, normal counts were observed in fertility assessments. These results represent the first in vivo evidence showing that USP26 is not essential for mouse gametogenesis.","PeriodicalId":10248,"journal":{"name":"Chromosoma","volume":"128 3","pages":"237-247"},"PeriodicalIF":1.6,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00412-019-00697-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37245092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18