H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos.

IF 2.5 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chromosoma Pub Date : 2023-11-01 Epub Date: 2023-09-12 DOI:10.1007/s00412-023-00809-3

Yakov A Osipov, Olga V Posukh, Darya A Kalashnikova, Polina A Antoshina, Petr P Laktionov, Polina A Skrypnik, Stepan N Belyakin, Prim B Singh

引用次数: 0

Abstract

Using a new method for bulk preparation of early stage embryos, we have investigated the role played by putative Planococcus citri H3K9 and H4K20 histone methyl transferases (HMTases) in regulating heterochromatinization of the imprinted paternal chromosomal set in male embryos. We found that H3K9 and H420 HMTases are required for heterochromatinization of the paternal chromosomes. We present evidence that both HMTases maintain the paternal "imprint" during the cleavage divisions when both parental chromosome sets are euchromatic. A testable model that accommodates our findings is proposed.

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H3K9和H4K20甲基转移酶直接参与了雄性柠檬扁球菌胚胎中父系染色体的异染色化。

我们采用一种新的早期胚胎批量制备方法，研究了推定的柠檬扁球菌 H3K9 和 H4K20 组蛋白甲基转移酶（HMTases）在调控雄性胚胎中父系染色体组印记异染色化中的作用。我们发现父系染色体的异染色质化需要 H3K9 和 H420 组蛋白甲基化酶。我们提出的证据表明，当亲本染色体组都是非染色体组时，这两种HMT酶能在裂殖分裂过程中维持父本 "印记"。我们提出了一个可检验的模型，以适应我们的发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromosoma 生物-生化与分子生物学

CiteScore

3.30

自引率

6.20%

发文量

审稿时长

1 months

期刊介绍： Chromosoma publishes research and review articles on the functional organization of the eukaryotic cell nucleus, with a particular emphasis on the structure and dynamics of chromatin and chromosomes; the expression and replication of genomes; genome organization and evolution; the segregation of genomes during meiosis and mitosis; the function and dynamics of subnuclear compartments; the nuclear envelope and nucleocytoplasmic interactions, and more. The scope of Chromosoma encompasses genetic, biophysical, molecular and cell biological studies. Average time from receipt of contributions to first decision: 22 days Publishes research and review articles on the functional organization of the eukaryotic cell nucleus Topics include structure and dynamics of chromatin and chromosomes; the expression and replication of genomes; genome organization and evolution; the segregation of genomes during meiosis and mitosis and more Encompasses genetic, biophysical, molecular and cell biological studies.