Clinica Chimica Acta最新文献_第8页

Paper-Based procalcitonin and Interleukin-6 test strip with Spectrum-Based optical reader for enterovirus severity differentiation in children 纸质降钙素原和白细胞介素-6 检测试纸条，带基于光谱的光学读取器，用于区分儿童肠道病毒的严重程度。

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-29 DOI: 10.1016/j.cca.2024.119942

Cheng-Han Chen , Rui-Ling Huang , Ching-Chuan Liu , Chitsung Hong , Yunching Chen , Ching-Fen Shen , Chao-Min Cheng

{"title":"Paper-Based procalcitonin and Interleukin-6 test strip with Spectrum-Based optical reader for enterovirus severity differentiation in children","authors":"Cheng-Han Chen , Rui-Ling Huang , Ching-Chuan Liu , Chitsung Hong , Yunching Chen , Ching-Fen Shen , Chao-Min Cheng","doi":"10.1016/j.cca.2024.119942","DOIUrl":"10.1016/j.cca.2024.119942","url":null,"abstract":"<div><p>Infectious diseases significantly impact global health, necessitating prompt diagnosis to mitigate life-threatening sepsis risk. Identifying patients at risk of severe neurological complications from enterovirus infections is challenging due to nonspecific initial presentations. Point-of-care testing (POCT) has emerged as a transformative tool, with low-cost lateral-flow colorimetric assays showing promise in deployable POCT devices. We developed a PCT/IL-6 rapid diagnostic system integrating lateral flow assay (LFA) test strips and a portable optical spectrum reader, allowing simultaneous semi-quantitative measurement of serum PCT and IL-6 within 30 min at the point of care. The system demonstrated a strong correlation with traditional ELISA and effectively differentiated severe pediatric enterovirus cases using serum samples. IL-6 showed superior discriminatory ability over PCT in identifying patients with severe neurological complications. This novel diagnostic platform holds great potential for early sepsis recognition and infectious disease management, especially in resource-limited settings.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of genetic polymorphisms in STIM1 and ORAI1 on erythropoietin resistance in Egyptian patients with end-stage renal disease STIM1和ORAI1基因多态性对埃及终末期肾病患者红细胞生成素耐受性的影响

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-28 DOI: 10.1016/j.cca.2024.119948

Azza A.E. Gomaa , Amany M.A. Zeid , Ibrahim M. Nagy , Ahmed M. Zahran

{"title":"The effect of genetic polymorphisms in STIM1 and ORAI1 on erythropoietin resistance in Egyptian patients with end-stage renal disease","authors":"Azza A.E. Gomaa , Amany M.A. Zeid , Ibrahim M. Nagy , Ahmed M. Zahran","doi":"10.1016/j.cca.2024.119948","DOIUrl":"10.1016/j.cca.2024.119948","url":null,"abstract":"<div><p>Chronic renal failure (CRF) is an incurable disease with unique challenges. Anemia is a frequent complication affecting dialysis patients. Erythropoietin (EPO) is used to treat anemia, but a poor response may result. We investigated genetic polymorphisms of store-operated calcium channel (SOC) signaling, an important erythropoietin-activated pathway that may induce EPO resistance in patients with renal failure. A total of 108 end stage renal disease (ESRD) patients were selected for this study. Patients were divided into two groups according to their erythropoietin resistance index (ERI): 39 patients with an ERI>10 and 69 patients with an ERI<10. We selected four tagging single nucleotide polymorphisms (tSNPs) in STIM1 and five in ORAI1 in our study. A polymerase chain reaction was performed, and genotyping against EPO resistance was correlated. Patients with the AG genotype of rs1561876 in STIM1, the TC genotype of rs6486795 in ORAI1, and the TG or GG genotypes of rs12320939 in ORAI1 were associated with an increased risk of erythropoietin resistance. Overall, we reported a moderately significant relationship between genetic polymorphisms of STIM1 and EPO resistance. We also reported a highly significant relationship between genetic polymorphisms of ORAI1 and EPO resistance. The (A-A-G) haplotype of STIM1 and the (G-T-G-T-A, G-C-G-C-G, or G-T-T-C-G) haplotypes of ORAI1 were significantly associated with EPO resistance.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNA-based electrochemical biosensors for ovarian cancer 基于 miRNA 的卵巢癌电化学生物传感器

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-28 DOI: 10.1016/j.cca.2024.119946

Saber Rouhi , Hassan Ghasemi , Mehdi Alizadeh , Ahmad Movahedpour , Farzaneh Vahedi , Mehdi Fattahi , Saleh Aiiashi , Seyyed Hossein Khatami

{"title":"miRNA-based electrochemical biosensors for ovarian cancer","authors":"Saber Rouhi , Hassan Ghasemi , Mehdi Alizadeh , Ahmad Movahedpour , Farzaneh Vahedi , Mehdi Fattahi , Saleh Aiiashi , Seyyed Hossein Khatami","doi":"10.1016/j.cca.2024.119946","DOIUrl":"10.1016/j.cca.2024.119946","url":null,"abstract":"<div><p>Ovarian cancer, a prevalent and deadly cancer among women, presents a significant challenge for early detection due to its heterogeneous nature. MicroRNAs, short non-coding regulatory RNA fragments, play a role in various cellular processes. Aberrant expression of these microRNAs has been observed in the carcinogenesis-related processes of many cancer types. Numerous studies highlight the critical role of microRNAs in the initiation and progression of ovarian cancer. Given their clinical importance and predictive value, there has been considerable interest in developing simple, prompt, and sensitive miRNA biosensor strategies. Among these, electrochemical sensors have demonstrated advantageous characteristics such as simplicity, sensitivity, low cost, and scalability. These microRNA-based electrochemical biosensors are valuable tools for early detection and point-of-care applications. This article discusses the potential role of microRNAs in ovarian cancer and recent advances in the development of electrochemical biosensors for miRNA detection in ovarian cancer samples.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow cytometry in acute myeloid leukemia and detection of minimal residual disease 流式细胞术在急性髓性白血病中的应用及最小残留病检测

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-28 DOI: 10.1016/j.cca.2024.119945

Qihui Cai , Haiqiang Lan , Deng Yi , Bojun Xian , Luo Zidan , Jianqiao Li , Zhaohong Liao

{"title":"Flow cytometry in acute myeloid leukemia and detection of minimal residual disease","authors":"Qihui Cai , Haiqiang Lan , Deng Yi , Bojun Xian , Luo Zidan , Jianqiao Li , Zhaohong Liao","doi":"10.1016/j.cca.2024.119945","DOIUrl":"10.1016/j.cca.2024.119945","url":null,"abstract":"<div><p>Acute myeloid leukemia (AML) is a common type of acute leukemia (AL), belonging to malignant tumors of the hematopoietic system with the characteristics of rapid disease development, control with extreme difficulties, easy recurrence, poor prognosis, and incidence rate increasing with age. The traditionally diagnostic standard of French American British (FAB), being based on the morphological examination with high human subjectivity, can no longer meet the demand of clinical diagnosis and treatment of AML. Requirements of objective accuracy and low-dose sample, have become the indispensable method for AML diagnosis and monitoring prognosis. Flow cytometry is a modern technology that can quickly and accurately detect the series, antigen distribution, differentiation stage of AML cells, minimal residual lesions after AML therapy, so as to provide the great significance in guiding clinical diagnosis, hierarchical treatment, and prognosis judgement. This article will systematically elaborate on the application of flow cytometry in the diagnosis and classification of AML, and the detection of minimal residual lesions, thereby providing reference significance for dynamic monitoring and prognostic observation of AML with different immune subtypes of FAB.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009898124021983/pdfft?md5=e5f34021762f825d30bbbc31696ee861&pid=1-s2.0-S0009898124021983-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coagulation factors VIII and factors IX testing practices in China: Results of the 5-year external quality assessment program 中国的凝血因子 VIII 和因子 IX 检测实践：为期 5 年的外部质量评估项目结果。

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-28 DOI: 10.1016/j.cca.2024.119950

Chengshan Xu, Zhongli Du, Gaofeng Hu, Yating Ma, Chenbin Li

{"title":"Coagulation factors VIII and factors IX testing practices in China: Results of the 5-year external quality assessment program","authors":"Chengshan Xu, Zhongli Du, Gaofeng Hu, Yating Ma, Chenbin Li","doi":"10.1016/j.cca.2024.119950","DOIUrl":"10.1016/j.cca.2024.119950","url":null,"abstract":"<div><h3>Background and aims</h3><p>Testing for coagulation factors VIII (FVIII) and IX (FIX) plays significant importance in the diagnostic and treatment of hemophilia A and B. External quality assessment (EQA) scheme aimed to assess the participants’ performance of testing for coagulation factors and identify shortcomings in clinical practice. This study aimed to investigate the performance trends of the participating laboratories in China national external quality assessment Scheme (China NEQAS) for FVIII and FIX over a five-year period (2019–2023).</p></div><div><h3>Materials and methods</h3><p>A total of ten external quality assessment (EQA) rounds were conducted from 2019 to 2023 in the China NEQAS for FVIII and FIX. The distribution of method, reagent and instrument were calculated. The trends of method- specific inter-laboratory coefficient of variation (CV) and pass rates were analyzed over 5 years. The dilutions for coagulation factor testing were also investigated.</p></div><div><h3>Results</h3><p>All laboratories use one-stage assays to detect FVIII and FIX activity. The inter-laboratory overall CV decreased year by year (10.9 % to 9.3 % for FVIII and 13.5 % to 10.2 % for FIX), and the laboratory pass rate steadily increased (88.0 % to 93.4 % for FVIII and 81.3 % to 92.7 % for FIX). The majority of laboratories employed a single dilution methodology for the assessment of FVIII and FIX activity. The interlaboratory CV was elevated for the Siemens reagent (Actin FSL) during analysis of moderately abnormal FIX concentrations of EQA samples in most batches.</p></div><div><h3>Conclusions</h3><p>The implementation of the external quality assessment has contributed to facilitate the enhancement of testing quality. Chromogenic assay is a supplement to accurate determination when necessary. Laboratories may choose to perform dilution tests or direct assays to identify the presence of inhibitors, particularly when they are suspected.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct analysis in real time mass spectrometry (DART-MS/MS) for rapid urine opioid detection in a clinical setting 直接分析实时质谱法（DART-MS/MS）用于在临床环境中快速检测尿液中的阿片类药物

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-27 DOI: 10.1016/j.cca.2024.119939

Ibrahim Choucair , Emily Shang , Minh Nguyet Tran, Gina Cassella-McLane, Joe M. El-Khoury

{"title":"Direct analysis in real time mass spectrometry (DART-MS/MS) for rapid urine opioid detection in a clinical setting","authors":"Ibrahim Choucair , Emily Shang , Minh Nguyet Tran, Gina Cassella-McLane, Joe M. El-Khoury","doi":"10.1016/j.cca.2024.119939","DOIUrl":"10.1016/j.cca.2024.119939","url":null,"abstract":"<div><h3>Background and aims</h3><p>Current laboratory methods for opioid detection involve an initial screening with immunoassays which offers efficient but non-specific results and a subsequent liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation which offers accurate results but requires extensive sample preparation and turnaround time. Direct Analysis in Real Time (DART) tandem mass spectrometry is evaluated as an alternative approach for accurate opioid detection with efficient sample preparation and turnaround time.</p></div><div><h3>Materials and methods</h3><p>DART-MS/MS was optimized by testing the method with varying temperatures, operation modes, extraction methods, hydrolysis times, and vortex times. The method was evaluated for 12 opioids by testing the analytical measurement range, percent carryover, precision studies, stability, and method-to-method comparison with LC-MS/MS.</p></div><div><h3>Results</h3><p>DART-MS/MS shows high sensitivity and specificity for the detection of 6-acetylmorphine, codeine, hydromorphone, oxymorphone, hydrocodone, naloxone, buprenorphine, norfentanyl, and fentanyl in urine samples. However, its performance was suboptimal for norbuprenorphine, morphine and oxycodone.</p></div><div><h3>Conclusion</h3><p>In this proof-of-concept study, DART-MS/MS is evaluated for its rapid quantitative definitive testing of opioids drugs in urine. Further research is needed to expand its application to other areas of drug testing.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142086847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological variation of methylmalonic acid in urine in spanish population 西班牙人口尿液中甲基丙二酸的生物变异。

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-25 DOI: 10.1016/j.cca.2024.119943

Maria Santes Berto , Sara Sanchez Asis , Juan Robles Bauza , Ana Rubio Alaejos , Josep Miquel Bauça , Jose Antonio Delgado

{"title":"Biological variation of methylmalonic acid in urine in spanish population","authors":"Maria Santes Berto , Sara Sanchez Asis , Juan Robles Bauza , Ana Rubio Alaejos , Josep Miquel Bauça , Jose Antonio Delgado","doi":"10.1016/j.cca.2024.119943","DOIUrl":"10.1016/j.cca.2024.119943","url":null,"abstract":"<div><h3>Background-Aim</h3><p>Methylmalonic acid (MMA) is currently the best biomarker of functional vitamin B12 deficiency. However, for a correct interpretation of the patient’s results it is necessary to know its biological variation (BV).</p><p>No BV data are available for urine MMA values, as measured by mass spectrometry. Hence, the aim of this study was to estimate the within- and between-person coefficients of variation (CV<sub>w,</sub> CV<sub>g</sub>) for MMA in a healthy population, and the associated index of individuality (II), as well as to define quality specifications based on BV and the reference change value (RCV).</p></div><div><h3>Methods</h3><p>Random urine samples from 34 healthy volunteers were collected over four consecutive weeks. Samples were stored at –80 °C until analysis in a single analytical run. MMA excretion was quantified by tandem liquid chromatography coupled to mass spectrometry (HPLC-MS/MS). Results were normalized to urine creatinine.</p><p>The coefficients of variation were estimated by CV-ANOVA. Confidence intervals (95 %) were calculated. Quality specifications were defined according to international recommendations.</p></div><div><h3>Results</h3><p>A total of 128 samples were included. The coefficients of variation were CV<sub>w</sub> = 35.7 % (26.1–45.3) and CV<sub>g</sub> = 67.7 % (58.3–77.0). The associated II was 0.5 and the RCV was 88.1 %.</p></div><div><h3>Conclusion</h3><p>Considering the II obtained, MMA in urine has high individuality, therefore, RCV is better to evaluate serial clinical results. Our results will contribute to a better clinical interpretation of this biomarker and will represent a great aid when defining analytical performance specifications for this magnitude.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cherry-red plasma: Beyond the assumption of hemolysis 樱桃红色血浆：超越溶血假设

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-23 DOI: 10.1016/j.cca.2024.119917

Lechuang Chen, Yu Zhang, Qing H. Meng

引用次数: 0

Development of an equation to predict delta bilirubin levels using machine learning 利用机器学习建立预测δ胆红素水平的方程

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-22 DOI: 10.1016/j.cca.2024.119938

Saejin Lee , Kwangjin Ahn , Taesic Lee , Jooyoung Cho , Moon Young Kim , Young Uh

{"title":"Development of an equation to predict delta bilirubin levels using machine learning","authors":"Saejin Lee , Kwangjin Ahn , Taesic Lee , Jooyoung Cho , Moon Young Kim , Young Uh","doi":"10.1016/j.cca.2024.119938","DOIUrl":"10.1016/j.cca.2024.119938","url":null,"abstract":"<div><h3>Objective</h3><p>Delta bilirubin (albumin-covalently bound bilirubin) may provide important clinical utility in identifying impaired hepatic excretion of conjugated bilirubin, but it cannot be measured in real-time for diagnostic purposes in clinical laboratories.</p></div><div><h3>Methods</h3><p>A total of 210 samples were collected, and their delta bilirubin levels were measured four times using high-performance liquid chromatography. Data collected included age, sex, diagnosis code, delta bilirubin, total bilirubin, direct bilirubin, total protein, albumin, globulin, aspartate aminotransferase, alanine transaminase, alkaline phosphatase, gamma-glutamyl transferase, lactate dehydrogenase, hemoglobin, serum hemolysis value, hemolysis index, icterus value (Iv), icterus index (Ii), lipemia value (Lv), and lipemia index. To conduct feature selection and identify the optimal combination of variables, linear regression machine learning was performed 1,000 times.</p></div><div><h3>Results</h3><p>The selected variables were total bilirubin, direct bilirubin, total protein, albumin, hemoglobin, Iv, Ii, and Lv. The best predictive performance for high delta bilirubin concentrations was achieved with the combination of albumin-direct bilirubin-hemoglobin-Iv-Lv. The final equation composed of these variables was as follows: delta bilirubin = 0.35 × Iv + 0.05 × Lv − 0.23 × direct bilirubin − 0.05 × hemoglobin − 0.04 × albumin + 0.10.</p></div><div><h3>Conclusion</h3><p>The equation established in this study is practical and can be easily applied in real-time in clinical laboratories.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009898124021910/pdfft?md5=d8f9a0967dfd2e83bbaef89765bed838&pid=1-s2.0-S0009898124021910-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142050304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergency department clinical performance of ADVIA Centaur n-terminal prohormone of B-type natriuretic peptide assay ADVIA Centaur B 型钠尿肽 n 端前体测定的急诊科临床表现。

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2024-08-22 DOI: 10.1016/j.cca.2024.119940

W. Frank Peacock , Jennifer Snyder , Chadwick Brown , Yan Liu Liu , Charles K Cooper , James L. Januzzi

{"title":"Emergency department clinical performance of ADVIA Centaur n-terminal prohormone of B-type natriuretic peptide assay","authors":"W. Frank Peacock , Jennifer Snyder , Chadwick Brown , Yan Liu Liu , Charles K Cooper , James L. Januzzi","doi":"10.1016/j.cca.2024.119940","DOIUrl":"10.1016/j.cca.2024.119940","url":null,"abstract":"<div><h3>Background</h3><p>Natriuretic peptide testing is guideline recommended as an aid to the diagnosis of heart failure (HF). We sought to evaluate the performance of the ADVIA Centaur (Siemens Healthcare Diagnostics, Tarrytown, NY) NT-proBNPII assay (PBNPII) in emergency department (ED) dyspneic patients.</p></div><div><h3>Methods</h3><p>Eligible patients presented to the ED with dyspnea, with their gold standard diagnosis determined by up to 3 cardiologists blinded to the PBNPII results. Patients were stratified into 3 groups based on PBNPII resultsa rule out group of NT-proBNP<300 pg/mL, an age-specific rule in group using cutoffs of 450, 900, and 1800 pg/mL, for <50, 50–75, and > 75 years respectively, and an intermediate cohort for results between the rule out and rule in groups.</p></div><div><h3>Results</h3><p>Of 3128 eligible patients, 1148 (36.7 %) were adjudicated as acute heart failure (AHF). The gold standard AHF diagnosis rate was 3.7, 24.3, and 67.2 % for patients with NTproBNPII in the negative, indeterminate, and positive groups, respectively. Overall likelihood ratios (LR) were 0.07 (95 % CI: 0.05,0.09), 0.55 (0.45,0.67), and 3.53 (3.26,3.83) for the same groups, respectively. Individual LR+for age dependent cutoffs were 5.01 (4.25,5.91), 3.71 (3.25,4.24), and 2.38 (2.10,2.69), respectively. NTproBNPII increased with increasing severity of HF when stratified by NYHA classification.</p></div><div><h3>Conclusions</h3><p>The ADVIA Centaur PBNPII assay demonstrates acceptable clinical performance using the recommended single rule out and age dependent rule in cutoffs for an AHF diagnosis in dyspneic ED patients.</p></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009898124021934/pdfft?md5=1d9fedc866e9eecd98efba18e241b40a&pid=1-s2.0-S0009898124021934-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0