Clinica Chimica Acta最新文献

{"title":"Urine organic acid metabolomic profiling by gas chromatography mass spectrometry: Assessment of solvent extract evaporation parameters on the recovery of key diagnostic metabolites","authors":"Rachel S. Carling ,&nbsp;Karolina Witek ,&nbsp;Erin C Emmett ,&nbsp;Claire Gallagher ,&nbsp;Stuart J. Moat","doi":"10.1016/j.cca.2024.120015","DOIUrl":"10.1016/j.cca.2024.120015","url":null,"abstract":"<div><h3>Background</h3><div>Analysis of urinary organic acids (UOAs) by gas chromatography mass-spectrometry (GC–MS) is widely used in metabolomic studies. It is a complex test with many limitations and pitfalls yet there is limited evidence in the literature to support best practice. This study investigated the impact of drying down time and temperature on the recovery of 16 key analytes from solvent extracts.</div></div><div><h3>Methods</h3><div>Pooled urine specimens were enriched with organic acids. Urine aliquots (n = 3) were acidified and extracted into diethyl ether and ethyl acetate. Extracts were dried under nitrogen at ambient temperature (25 °C); 40 °C; 60 °C then left for 0; +5; +15 min. Dried extracts were derivatised with N,O,-bis-(trimethylsilyl)trifluoroacetamide prior to analysis by GC–MS. Urine specimens from individuals with biotinidase deficiency, maple syrup urine disease (MSUD) and ketotic hypoglycemia were analysed to demonstrate the potential clinical impact.</div></div><div><h3>Results</h3><div>Recovery of shorter chain hydroxycarboxylic acids decreased significantly when extracts were dried above 25 °C (mean recovery 89 % at 60 °C, p &lt; 0.01) or left under nitrogen post-drying (mean recovery at ambient + 15 min, 40 °C + 15mins and 60 °C + 15mins was 56 %, 12 % and 2 %, respectively, p &lt; 0.01). Whilst dicarboxylic acids/medium chain fatty acids were unaffected by temperature (mean recovery 100 %), prolonged drying reduced recovery (mean recovery 85 % at 60 °C + 15mins, p &lt; 0.01).</div></div><div><h3>Conclusions</h3><div>Evaporation of solvent extracts with heat and/or prolonged drying under nitrogen results in significant losses of the shorter chain hydroxycarboxylic acids. The evaporation protocol must be carefully controlled to ensure accurate and reproducible results, preventing misdiagnoses and/or misinterpretation of results.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120015"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood biomarkers for Alzheimer’s disease with the Lumipulse automated platform: Age-effect and clinical value interpretation","authors":"Giulia Musso ,&nbsp;Carlo Gabelli ,&nbsp;Marco Puthenparampil ,&nbsp;Chiara Cosma ,&nbsp;Annachiara Cagnin ,&nbsp;Paolo Gallo ,&nbsp;Gianni Sorarù ,&nbsp;Elena Pegoraro ,&nbsp;Martina Zaninotto ,&nbsp;Angelo Antonini ,&nbsp;Stefania Moz ,&nbsp;Carlo Federico Zambon ,&nbsp;Mario Plebani ,&nbsp;Maurizio Corbetta ,&nbsp;Daniela Basso","doi":"10.1016/j.cca.2024.120014","DOIUrl":"10.1016/j.cca.2024.120014","url":null,"abstract":"<div><h3>Background</h3><div>Advances in analytical methods have recently paved the way to Alzheimer’s disease (AD) biomarkers testing in blood along with the more established CSF testing. To ensure a forthcoming application of this low-invasive diagnostic that might allow to recognize early onset of dementia, appropriate pathological cut-points need to be defined.</div></div><div><h3>Methods</h3><div>In this cross-sectional study we measured blood and CSF neurofilament light chain (NFL), phosphorylated tau (pTau 181), Amyloid-β1-42 (AB 1–42) and Amyloid-β1-40 (AB 1–40) on a fully automated chemiluminescent platform (Lumipulse, Fujirebio) in 80 cognitively impaired patients and 55 cognitively unimpaired subjects. Clinical cut points were calculated with receiver-operator characteristic (ROC) curve analysis and a head-to-head comparison of blood and CSF testing was performed.</div></div><div><h3>Results</h3><div>Blood NFL best discriminant thresholds to distinguish neurodegenerative diseases from controls varied age-dependently, being 19 and 33 pg/mL in subjects 50–65 years and &gt; 65 years respectively. AD was best framed by AB 1–42/1–40 ratio &lt; 0.079 and ptau181 &gt; 1 pg/mL. Though a strong correlation for all biomarkers, only blood AB ratio was equal to CSF testing for AD diagnosis.</div></div><div><h3>Conclusions</h3><div>The specific context of use might be considered to define the cut-offs of blood biomarkers of neurodegenerative diseases. Future efforts towards reference materials for each AD blood biomarker will improve clinical cut-offs.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120014"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanodiagnostics in global eradication of hepatitis C virus","authors":"Mohammad Darvishi ,&nbsp;Reza Amiri ,&nbsp;Emad Ghannad ,&nbsp;Samir Mehrabkhani ,&nbsp;Nassim Rastgar ,&nbsp;Mahkameh Razaghi ,&nbsp;Jaya Bansal ,&nbsp;Mamata Chahar ,&nbsp;Pranchal Rajput ,&nbsp;Hossein Saffarfar ,&nbsp;Payam Ali-Khiavi ,&nbsp;Ahmad Mobed ,&nbsp;Yalda Yazdani","doi":"10.1016/j.cca.2024.120013","DOIUrl":"10.1016/j.cca.2024.120013","url":null,"abstract":"<div><div>Hepatitis C, caused by the hepatitis C virus (HCV), is a prevalent liver disease with severe outcomes, including cirrhosis and hepatocellular carcinoma. Traditional diagnostic methods primarily detect antiviral antibodies (anti-HCV) or viral RNA, but these approaches have limitations. Anti-HCV antibodies may take 2–4 weeks to develop in acute cases and can be absent in some individuals, leading to undiagnosed early-stage infections. This poses significant challenges for public health, particularly in resource-limited settings where early detection is crucial. This article explores the development of biosensors engineered to directly detect HCV surface antigens, such as envelope proteins. These biosensors provide a promising solution for earlier and more accurate diagnosis by identifying viral components at the initial stages of infection. By focusing on direct detection of viral antigens, these innovations could enhance early diagnosis, facilitate timely intervention, and reduce virus transmission. We evaluate the advancements in biosensor technology over the past decade and their potential to improve HCV detection in clinical and field settings, ultimately supporting global efforts to eliminate HCV as a public health threat.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120013"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian cancer extracellular vesicle biomarkers","authors":"Zeinab Jamali ,&nbsp;Masoumeh Razipour ,&nbsp;Mahsa Zargar ,&nbsp;Hojat Ghasemnejad-Berenji ,&nbsp;Seyed Mohammad Akrami","doi":"10.1016/j.cca.2024.120011","DOIUrl":"10.1016/j.cca.2024.120011","url":null,"abstract":"<div><div>Ovarian cancer (OC) remains a significant women’s health concern due to its high mortality rate and the challenges posed by late detection. Exploring novel biomarkers could lead to earlier, more specific diagnoses and improved survival rates for OC patients. This review focuses on biomarkers associated with extracellular vesicles (EVs) found in various proximal fluids, including urine, ascites, utero-tubal lavage fluid of OC patients. We highlight these proximal fluids as rich sources of potential biomarkers. The review explains the roles of EV biomarkers in ovarian cancer progression and discusses EV-related proteins and miRNAs as potential diagnostic or prognostic indicators and therapeutic targets. Finally, we highlighted the limitations of examining proximal fluids as sources of biomarkers and encourage researchers to proactively pursue innovative solutions to overcome these challenges.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120011"},"PeriodicalIF":3.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moving metabolomics into the routine of clinical laboratories: A forward-thinking strategy","authors":"Michele Mussap","doi":"10.1016/j.cca.2024.120012","DOIUrl":"10.1016/j.cca.2024.120012","url":null,"abstract":"","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120012"},"PeriodicalIF":3.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real world feasibility of patient-based real time quality control (PBRTQC) using five analytes in a South African laboratory","authors":"Sheromna Sewpersad ,&nbsp;Bettina Chale-Matsau ,&nbsp;Tahir S. Pillay","doi":"10.1016/j.cca.2024.120006","DOIUrl":"10.1016/j.cca.2024.120006","url":null,"abstract":"<div><h3>Background</h3><div>Patient-based real-time quality control (PBRTQC) identifies possible bias in methods by utilising shifts in trend of statistical measures in laboratory results. In this study we aimed to compare and optimize various PBRTQC procedures for serum alanine aminotransferase, albumin, calcium, ferritin and sodium.</div></div><div><h3>Methods</h3><div>In a bias simulation study, we added artificial bias to intervals of patient data and then evaluated the efficiency with which various PBRTQC procedures were able to detect this bias. PBRTQC procedures used included block size, moving statistic calculation, control limits as well as truncation limits. The number of patients till error detection, the false alarm rate as well as validation charts were utilised to select the optimal PBRTQC procedure for each analyte.</div></div><div><h3>Results</h3><div>The optimal PBRTQC procedures identified for each analyte were: ALT − MA T0 50 MaxMin; Albumin − MA T5 100 Perc; Calcium − MM T5 50 Perc; Ferritin − MA T0 100 MaxMin; and Sodium − MA T5 75 MaxMin. (T, Truncation limits; Control limits- MA, Moving Average, MM, Moving median; Perc, percentile)</div></div><div><h3>Conclusions</h3><div>The use of large, real patient datasets allows for the reliable determination of laboratory-specific PBRTQC procedures. This study demonstrates that the moving average calculation excels in both normal and transformed analyte distributions. Whilst, the benefits of PBRTQC procedures are proven, the complex and time-consuming optimisation process may be a barrier to the rapid implementation in under-resourced countries.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120006"},"PeriodicalIF":3.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics identifies metabolite markers in plasma and extracellular vesicles within plasma in patients with asthma","authors":"Chih-Jung Chang ,&nbsp;Qi-Wen Ma ,&nbsp;Tian-Lin Li ,&nbsp;Jun-An Liu ,&nbsp;Cheng-Hsien Hsieh ,&nbsp;Liang Chen","doi":"10.1016/j.cca.2024.120010","DOIUrl":"10.1016/j.cca.2024.120010","url":null,"abstract":"<div><h3>Background</h3><div>Plasma and extracellular vesicles (EVs) derived from plasma are important sources of information regarding individual health. Metabolomic analysis of plasma and EVs may provide new methods for predicting disease occurrence. This study aims to analyze the metabolomic characteristics of plasma and plasma EVs in asthma patients.</div></div><div><h3>Methods</h3><div>Plasma samples were collected from healthy individuals and asthma patients. EVs were isolated from the plasma using ultracentrifugation. The isolated EVs were characterized by nanoparticle tracking analysis and flow cytometry. Metabolomic analysis was performed using a liquid chromatography-mass spectrometry platform.</div></div><div><h3>Results</h3><div>This study successfully extracted EVs from plasma samples. Metabolomic analysis revealed that the composition of differential metabolites in the plasma and EVs of asthma patients was similar. KEGG pathway analysis indicated that the number of upregulated metabolic pathways enriched with differential metabolites in the plasma EVs of asthma patients was significantly greater than that in the plasma samples. Pathways associated with the onset of asthma included asthma, systemic lupus erythematosus, glycerophospholipid metabolism, and autophagy – other, primarily involving the following five metabolites: PS(18:1(9Z)/18:2(9Z,12Z)), PC(18:1(9Z)e/2:0), PS(24:1(15Z)/22:2(13Z,16Z)), PE(22:4(7Z,10Z,13Z,16Z)/22:5(4Z,7Z,10Z,13Z,16Z)), and PE(16:0/20:3(8Z,11Z,14Z)). Receiver operating characteristic analysis results suggested that these five differential metabolites may serve as potential biomarkers for asthma.</div></div><div><h3>Conclusion</h3><div>We identified the metabolic characteristics of plasma and EVs in asthma patients, confirming that the metabolites in plasma EVs may serve as potential biomarkers for asthma. This finding not only enhances our understanding of the pathogenesis of asthma but also opens new avenues for targeted therapy.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120010"},"PeriodicalIF":3.2,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transplant patient classification based on everolimus blood concentrations: Is there a risk of “misclassifications” using immunoassays?","authors":"Anne-Sophie Bargnoux ,&nbsp;Thibault Sutra ,&nbsp;Stéphanie Badiou ,&nbsp;Pierre-Edouard Grillet ,&nbsp;Anne-Marie Dupuy ,&nbsp;Ilan Szwarc ,&nbsp;Georges-Philippe Pageaux ,&nbsp;Moglie Le Quintrec ,&nbsp;Jean-Paul Cristol","doi":"10.1016/j.cca.2024.120009","DOIUrl":"10.1016/j.cca.2024.120009","url":null,"abstract":"","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120009"},"PeriodicalIF":3.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics detection and treatment of syphilis","authors":"Xinyan Shi ,&nbsp;Jiayin Shi ,&nbsp;Fei Zou ,&nbsp;Qian Cao ,&nbsp;Xiaoliang Yan ,&nbsp;Shuangquan Liu ,&nbsp;Yumeng Li ,&nbsp;Xiaopeng Lan","doi":"10.1016/j.cca.2024.120008","DOIUrl":"10.1016/j.cca.2024.120008","url":null,"abstract":"<div><div><em>Treponema pallidum</em> is the source of the chronic systemic sexually transmitted illness syphilis. <em>T. pallidum</em> can evade immunity and spread. A hard chancre, enlarged lymph nodes, and a syphilis rash are the primary clinical signs. The condition may affect the nervous or cardiovascular system and even become fatal after being neglected. Omics technology is a cutting-edge technique that maps the entire regulatory network of gene and protein metabolism using high-throughput sequencing and other techniques, such as transcriptomics, proteomics, metabolomics, and genomics, to perform more efficient and methodical research on biological samples. Owing to the diverse and intricate biological roles and gene expression of <em>T. pallidum</em>, a single omics study is frequently insufficient and limited. This review focused on and summarized the use of several omics methods for investigating <em>T. pallidum</em> by referencing several different studies in the literature.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120008"},"PeriodicalIF":3.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of 3-O-methyldopa from dried plasma microsamples by high-resolution mass spectrometry: A tool for the diagnosis of patients with high-risk neuroblastoma","authors":"Margherita Biondi ,&nbsp;Sebastiano Barco ,&nbsp;Davide Cangelosi ,&nbsp;Alessia Cafaro ,&nbsp;Martina Morini ,&nbsp;Federica Pigliasco ,&nbsp;Lucilla Rossi ,&nbsp;Fabrizio Mancin ,&nbsp;Massimo Conte ,&nbsp;Alberto Garaventa ,&nbsp;Giuliana Cangemi","doi":"10.1016/j.cca.2024.120005","DOIUrl":"10.1016/j.cca.2024.120005","url":null,"abstract":"<div><h3>Background</h3><div>Risk assessment at diagnosis is crucial for neuroblastoma (NB) in order to address patients at high-risk to the most timely and appropriate treatments. 3-O-methyldopa (3-OMD), a direct metabolite of L-Dopa, is a promising biomarker of NB at diagnosis able to stratify high-risk patients.</div></div><div><h3>Methods</h3><div>We show the development and validation of a method for measuring 3-OMD from dried plasma samples (DPS) and plasma using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) on a Thermo Fisher Scientific Orbitrap Exploris 120.</div></div><div><h3>Results</h3><div>The method was accurate and reproducible in the range 7.8–4000 ng/mL, from small amounts (50 mL) of plasma and DPS (obtained starting from 30 mL plasma). 3-OMD concentrations measured in plasma and DPS were highly correlated (R = 0.99 95 %CI 0.993–0.996). Differences of 3-OMD levels across stages L1 and M and L1 and L2 (p-value &lt; 0.05) were statistically significant. Receiving Operator Curve (ROC) analysis showed that 3-OMD was able to discriminate patients at high-risk with high sensitivity and specificity both from plasma or DPS (AUC = 0.8295 %CI 0.71–0.94, P &lt; 0.0001).</div></div><div><h3>Conclusions</h3><div>3-OMD is confirmed as an interesting biomarker of high-risk NB. The described method is an added value for further prospective studies involving multiple sites. The stability of 3-OMD in DPS allows for easy shipment and storage at room temperature.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"565 ","pages":"Article 120005"},"PeriodicalIF":3.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}