Clinica Chimica Acta最新文献

{"title":"The role of miR-21 as a biomarker and therapeutic target in cardiovascular disease","authors":"Fanlong He ,&nbsp;Wenqing Guan","doi":"10.1016/j.cca.2025.120304","DOIUrl":"10.1016/j.cca.2025.120304","url":null,"abstract":"<div><div>Cardiovascular diseases (CVDs) are the leading causes of death worldwide, accounting for a significant burden on global health systems. The complexity of CVDs arises from their multifactorial etiology, including genetic, environmental, and lifestyle factors. Early diagnosis and effective treatment remain critical for reducing mortality and improving patient outcomes, yet conventional methods often fall short in providing precise, timely information for disease management. MicroRNAs are small non-coding RNAs that regulate gene expression and play pivotal roles in various biological processes, including cardiovascular health. Among them, miR-21 has garnered significant attention due to its involvement in cardiac remodeling, fibrosis, inflammation, and hypertrophy. Elevated levels of miR-21 are frequently observed in conditions such as myocardial infarction, heart failure, and coronary artery disease, positioning it as a potential biomarker for early detection and disease progression. Furthermore, therapeutic strategies targeting miR-21, such as antagomirs and innovative delivery systems, have shown promise in preclinical studies, though challenges like off-target effects and delivery inefficiencies persist. This review aims to provide a comprehensive understanding of miR-21′s role in CVDs, addressing its potential as a diagnostic biomarker and therapeutic target. We discuss recent advancements, limitations, and future prospects in miR-21 research, emphasizing the importance of integrating this knowledge into clinical practice to improve CVD management.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120304"},"PeriodicalIF":3.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics in decoding cancer: A review","authors":"Elaheh Gheybi ,&nbsp;Pejman Hosseinzadeh ,&nbsp;Vahid Tayebi-Khorrami ,&nbsp;Mehdi Rostami ,&nbsp;Mohammad Soukhtanloo","doi":"10.1016/j.cca.2025.120302","DOIUrl":"10.1016/j.cca.2025.120302","url":null,"abstract":"<div><div>Cancer remains the second leading cause of death worldwide, posing a significant global health challenge. Extensive research has revealed common biological characteristics across cancer cells, forming the foundation for developing innovative diagnostic and therapeutic strategies. To better understand these shared traits, advanced measurement technologies are critical. Proteomics, the large-scale study of proteins and their functions, has emerged as a transformative tool for uncovering the complexities of cancer biology. This approach provides an in-depth view of cellular activities and protein interactions, offering unprecedented insights into cancer progression and treatment. Unlike traditional methods that investigate specific pathways in isolation, proteomics enables simultaneous analysis of thousands of proteins, generating a comprehensive understanding of cancer biology. This review explores the mechanisms underlying proteomics, its application to understanding cancer hallmarks, and its potential to transform clinical approaches. By examining proteomics’ role in metastasis, angiogenesis, proliferation, and resistance mechanisms, this study highlights its contributions to cancer diagnosis, treatment, and personalized medicine. Additionally, prospects in integrating proteomics with other −omics fields and advancements in computational analysis will be discussed. This work aims to illuminate the path toward more effective, precise, and individualized cancer care through proteomics innovations.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120302"},"PeriodicalIF":3.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical implications of serum adropin and clusterin in chronic renal failure patients who received hemodialysis","authors":"Feng Gu ,&nbsp;Yanfeng Wu ,&nbsp;Jingyuan Lu ,&nbsp;Penghui Zhang ,&nbsp;Hualin Qi","doi":"10.1016/j.cca.2025.120287","DOIUrl":"10.1016/j.cca.2025.120287","url":null,"abstract":"<div><h3>Background</h3><div>Both Adropin and Clusterin are associated with cardiovascular and cerebrovascular diseases. Unfortunately, the clinical implications of them in Chronic renal failure (CRF) population remains largely unclear.</div></div><div><h3>Methods</h3><div>We included 356 CRF patients received hemodialysis. Determination of serum concentrations of Adropin and Clusterin were performed by ELISA.The optimal cutoff values of Adropin or Clusterin for cardiovascular and cerebrovascular complications/death were determined by receiver operating characteristics (ROC) curve analysis. The prognostic value of Adropin and Clusterin was evaluated using Kaplan–Meier survival curves, log-rank tests, and Cox proportional hazards models.</div></div><div><h3>Results</h3><div>Baseline serum Adropin significantly decreased, while serum Clusterin elevated in CRF patients who suffered cardiovascular or cerebrovascular implications during hemodialysis. Both decreased baseline serum Adropin and increased serum Clusterin showed potentials for predicting cardiovascular/cerebrovascular implications during hemodialysis in CRF patients. Reduced baseline serum Adropin indicated poor prognosis in CRF patient during hemodialysis, whereas increased baseline serum Clusterin indicated poor prognosis in CRF patient during hemodialysis. Combining baseline serum Adropin and Clusterin detection achieved better performance for predicting prognosis in CRF patients.</div></div><div><h3>Conclusion</h3><div>We demonstrated that decreased Adropin, or increased Clusterin could be novel but useful tool for predicting cardiovascular/cerebrovascular complications and overall survival. Importantly, combination of Adropin and Clusterin detection might be help to construct new management system for CRF patients to effectively improve their prognosis in the future.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120287"},"PeriodicalIF":3.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early detection of unusual cryoglobulinemia from automated cell counts and blood films","authors":"Bob Smit ,&nbsp;Ilse JE Kouijzer ,&nbsp;Wilbert AG van der Meijden ,&nbsp;Ayden Kleij ,&nbsp;Corrie M de Kat Angelino ,&nbsp;Charissa Wijnands ,&nbsp;Laura Belt ,&nbsp;Annette Vendel ,&nbsp;Raphaël Duivenvoorden ,&nbsp;Joannes FM Jacobs","doi":"10.1016/j.cca.2025.120290","DOIUrl":"10.1016/j.cca.2025.120290","url":null,"abstract":"<div><div>Cryoglobulins are circulating immunoglobulins characterized by reversible cold-induced precipitation. The process of serum cryoprecipitation, performed at 4 °C, takes three to seven days, which leads to delays in obtaining cryoglobulinemia lab-results. Here, we report two cases of cryoglobulinemia, each with distinct cryoglobulin-induced features that were identifiable through automated hemocytometry and blood smear analysis. The blood smear showed neutrophilic inclusions and amorphous extra-cellular material, and the automated hematology analyzer displayed abnormal scatterplots. All these unique features positively correlated with the duration the blood sample was kept at room temperature prior to analysis. These hemocytometric abnormalities may serve as early indicators for the diagnosis of cryoglobulinemia, potentially aiding the detection of this condition.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120290"},"PeriodicalIF":3.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immuno-PCR: A high-sensitivity approach for biomarker analysis","authors":"Hansen Shi ,&nbsp;Biyun Zeng ,&nbsp;Qiping Wei ,&nbsp;Zhu Yuan ,&nbsp;Jingjie Peng ,&nbsp;Peijun Zhang ,&nbsp;Tiancai Liu ,&nbsp;Tao Zeng","doi":"10.1016/j.cca.2025.120289","DOIUrl":"10.1016/j.cca.2025.120289","url":null,"abstract":"<div><div>The integration of immunology and molecular biology, Immuno-Polymerase Chain Reaction, ie, Immuno-PCR, (iPCR), is an innovative cutting-edge detection strategy that holds significant promise for the identification of pathophysiologic biomarkers present at very low concentration or those inherently unstable. iPCR, known for superior sensitivity and specificity, has proven valuable in early disease diagnosis, monitoring and prognosis. This review summarizes the current applications of iPCR in detecting various disease biomarkers including those related to cancer, infection, autoimmune, cardiovascular, and neurological disease. We introduce the principle, advantages and limitations, specific applications, and clinical significance of iPCR, thereby promoting the widespread application of this technology in disease diagnosis. This technology facilitates early detection and intervention, enhances patient outcomes and survival rates, and is a valuable reference for future research and applications.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120289"},"PeriodicalIF":3.2,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uniformity and stability of saliva composition based on glucose concentration analysis","authors":"Kuncheng Chen ,&nbsp;Song Wang ,&nbsp;Pian Zhong ,&nbsp;Yeping Peng ,&nbsp;Junzhe Lu ,&nbsp;Lanlan Liu ,&nbsp;Jinmei He ,&nbsp;Weiqiang Liu","doi":"10.1016/j.cca.2025.120283","DOIUrl":"10.1016/j.cca.2025.120283","url":null,"abstract":"<div><div>Saliva holds promise as a biomarker for glucose monitoring due to its non-invasive and self-collection characteristics. However, its viscosity may lead to uneven glucose distribution, reflecting the uniformity of saliva, and individual glycemic control factors such as diet may affect salivary glucose concentration, reflecting the stability of saliva. To address these concerns, this study aims to evaluate the uniformity and stability of salivary glucose concentrations.</div><div>Macro-rheological properties (shear viscosity, elastic modulus, and viscous modulus) and micro-morphological characteristics of saliva were analyzed using a rheometer and Scanning Electron Microscopy and Energy-Dispersive X-ray Spectroscopy (SEM-EDS) techniques. Salivary glucose uniformity and stability were assessed under room-temperature static conditions, fasting saliva over a one-month period, and in postprandial states.</div><div>Results showed that saliva exhibits shear-thinning behavior, with shear viscosity decreasing from 0.1 Pa∙s to 0.001 Pa∙s as shear rate increases from 0.1 to 150 s⁻¹. This shear-thinning property, linked to the porous glycoprotein network observed through micro-morphological analysis, may influence analyte distribution. Despite the inherent viscosity, salivary glucose concentrations demonstrated high uniformity: 89 % of fasting samples and 92 % of postprandial samples showed less than 10 % variation when divided into three portions.</div><div>Regarding stability, salivary glucose levels in healthy individuals remained consistently more stable than those in diabetic patients across various conditions, including static room-temperature storage of fasting saliva, fasting saliva over one month, and postprandial saliva. For example, fasting glucose levels over one month in diabetic patients (7.38 ± 6.3 mg/dL) were higher and less stable than those observed in healthy individuals (1.674 ± 0.72 mg/dL).</div><div>This study confirms that fasting salivary glucose is both homogeneous and sufficiently stable, supporting its potential as a viable biomarker for blood glucose monitoring.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120283"},"PeriodicalIF":3.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prospective cohort study on serum PINK1 as a biochemical marker in relation to poor neurological prognosis, stroke-associated pneumonia and early neurological deterioration after acute intracerebral hemorrhage","authors":"Weifang Ni,&nbsp;Si-Hua Chen,&nbsp;Le Dai,&nbsp;Yifu Zhou,&nbsp;Chenjun He","doi":"10.1016/j.cca.2025.120282","DOIUrl":"10.1016/j.cca.2025.120282","url":null,"abstract":"<div><h3>Background</h3><div>PTEN-induced putative kinase 1 (PINK1) may moderate neurodegeneration via sustaining mitochondrial function and integrity. Here, we attempted to determine relationship between serum PINK1 levels, disease severity, poor prognosis, Early Neurological Deterioration (END) and Stroke-Associated Pneumonia (SAP) following acute Intracerebral Hemorrhage (ICH).</div></div><div><h3>Methods</h3><div>Altogether, 175 patients with ICH and 80 controls were encompassed in this prospective cohort study. Serum PINK1 levels were measured at admission of all patients and at study entry of all controls. The National Institutes of Health Stroke Scale (NIHSS) scores and hematoma volumes were applied to determine the severity. SAP, END and post-ICH 6-month poor prognosis (modified Rankin Scale scores: 3–6) were recorded as the three outcome variables of interest.</div></div><div><h3>Results</h3><div>Patients, in contrast to controls, had significantly elevated serum PINK1 levels. Serum PINK1 levels were independently correlated with NIHSS scores, hematoma volumes and 6-month modified Rankin Scale scores. Serum PINK1 levels were linearly correlated with risks of SAP, END and post-ICH 6-month poor prognosis under the restricted cubic spline, as well as along with NIHSS scores and hematoma volumes, became their independent predictors. As demonstrated under receiver operating characteristic curve, serum PINK1 levels displayed effective predictive ability and possessed similar discrimination efficiency, when compared to NIHSS scores and hematoma volumes. Using sensitivity analysis, prognosis association was robust.</div></div><div><h3>Conclusion</h3><div>Serum PINK1 levels are substantially heightened after ICH, and may accurately mirror hemorrhagic intensity and efficaciously forecast END, SAP, and poor neurological prognosis, signifying that PINK1 may be a serological prognosticator of good prospect in ICH.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120282"},"PeriodicalIF":3.2,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of Klebsiella pneumoniae based on one-tube RPA-CRISPR/Cas12a system","authors":"Zhiyun Wu ,&nbsp;Yin Xu ,&nbsp;Wei Zhou ,&nbsp;Luoluo Shi ,&nbsp;Weifeng Shi ,&nbsp;Lei Pu ,&nbsp;Jingting Jiang","doi":"10.1016/j.cca.2025.120281","DOIUrl":"10.1016/j.cca.2025.120281","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> (KP) is a prevalent pathogen implicated in both community-acquired and nosocomial infections, often leading to severe clinical outcomes. The conventional methods for KP identification are characterized by intricacy and suboptimal efficiency. In this research, we have engineered a novel One-Tube RPA- CRISPR/Cas12a system, integrating recombinase polymerase amplification (RPA) method with the CRISPR/Cas12a diagnostic platform, to facilitate the detection of <em>K. pneumoniae</em>. To minimize the likelihood of aerosol-based contamination, the RPA components are positioned at the base of the tube, while the CRISPR/Cas12a components are placed at the tube’s cap. The systems are combined post-RPA amplification through a brief centrifugation step, ensuring that RPA reactions are conducted independently to produce an adequate amount of target DNA before interaction with the CRISPR/Cas12a system. This method was validated using both fluorescent and lateral flow strip assays, achieving a limit of detection (LOD) of 10⁰ copies/μL and 10¹ copies/μL respectively. The specificity for KP detection was found to be 100 %. Furthermore, the system demonstrated a positivity rate of 78 % (18/23) when directly extracting DNA from sputum samples, corroborated by culture and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The simplicity and rapidity of the assay are augmented by a straightforward sample processing without extraction. The complete assay duration from specimen receipt to result is approximately 40 min, significantly reducing the turnaround time (TAT). Collectively, this system presents a streamlined, expeditious, and highly specific diagnostic approach for the detection of <em>Klebsiella pneumoniae</em> strains.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120281"},"PeriodicalIF":3.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective multicenter comparison of the diagnostic value of IgG and IgGAM antibodies for heparin-induced thrombocytopenia","authors":"Wenjie Zhang ,&nbsp;Rui Feng ,&nbsp;Yu Su ,&nbsp;Jia Kang ,&nbsp;Yong Yao ,&nbsp;Huiru Zhao ,&nbsp;Xiangyu Cao ,&nbsp;Qingkun Fan ,&nbsp;Jun Wu","doi":"10.1016/j.cca.2025.120279","DOIUrl":"10.1016/j.cca.2025.120279","url":null,"abstract":"<div><h3>Background and aims</h3><div>Heparin-induced thrombocytopenia (HIT) is a critical complication of heparin therapy. Immune antibodies are involved in the development of HIT. The purpose of this study was to explore the diagnostic value of IgGAM and IgG antibodies.</div></div><div><h3>Materials and methods</h3><div>This prospective multicenter cohort study enrolled 170 patients with suspected HIT and 120 healthy volunteers. HIT was suspected when the complete blood count indicated a reduced platelet count. The presence of HIT was determined according to the clinical condition and laboratory results of patients. Parameters examined included conventional coagulation tests and IgG and IgGAM antibodies.</div></div><div><h3>Results</h3><div>Among 170 patients with suspected HIT, 69 were PF4/H-antibody-positive (Ab+ patients), and 12 were diagnosed with HIT. Ab+ patients had higher levels of 4T score, D-dimer, and platelet reduction ratio than Ab- patients (<em>P</em> &lt; 0.001 for all). We defined a HIT antibody risk index (HARI) as 0.807 × 4T score+ 0.028 × platelet reduction ratio (%)+ 0.114 × D-dimer (mg/l FEU), and found that the area under the curve (AUC) for HARI was greatest (0.857). Furthermore, the levels of IgGAM and IgG antibodies were higher in patients with HIT than without (<em>P</em> = 0.003 and <em>P</em> &lt; 0.001, respectively). The AUC for IgG antibody was significantly greater than that for IgGAM antibody (0.870 vs. 0.775).</div></div><div><h3>Conclusions</h3><div>HARI can serve as a potential predictor for the risk of PF4/H-antibody positivity in patients with suspected HIT pre-test. For high-risk patients, IgG antibody testing may be used as a potential exclusion diagnostic indicator for patients with suspected HIT.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120279"},"PeriodicalIF":3.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considerations for enhancing the clinical validation and comparative analysis of RPA-CRISPR-Cas12a in Mycoplasma pneumoniae detection","authors":"Shanshan Yuan,&nbsp;Congcong Cheng","doi":"10.1016/j.cca.2025.120274","DOIUrl":"10.1016/j.cca.2025.120274","url":null,"abstract":"","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"572 ","pages":"Article 120274"},"PeriodicalIF":3.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}