Clinica Chimica Acta最新文献

{"title":"Corrigendum to “Unveiling the biological variation of lymphocyte subset counts: Insights from a full-spectrum flow cytometer and the BD Multitest™ 6-Color TBNK kit” [Clin. Chim. Acta 569 (2025) 120152]","authors":"Kai Guo , Xiaoran Feng , Lei Xu , Zhongli Du , Yating Ma , Hong Lu , Chenbin Li , Mingting Peng","doi":"10.1016/j.cca.2025.120284","DOIUrl":"10.1016/j.cca.2025.120284","url":null,"abstract":"","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120284"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neoplastic hypercortisolism: Cut-off values for late-night salivary cortisol & cortisone","authors":"Karlijn Koops ,&nbsp;Peter H. Bisschop ,&nbsp;Annemieke C. Heijboer ,&nbsp;Jacquelien J. Hillebrand","doi":"10.1016/j.cca.2025.120310","DOIUrl":"10.1016/j.cca.2025.120310","url":null,"abstract":"<div><h3>Objectives</h3><div>One of the recommended initial tests for diagnosing neoplastic hypercortisolism is the measurement of late-night salivary cortisol (Cortisol_LNSa). Published cut-off values for Cortisol_LNSa and late-night salivary cortisone (Cortisone_LNSa) vary widely between studies. This study aims to establish cut-off values for Cortisol_LNSa and Cortisone_LNSa using liquid chromatography-tandem mass spectrometry and to determine which salivary hormone performs better in distinguishing between people with and without neoplastic hypercortisolism.</div></div><div><h3>Methods</h3><div>A retrospective study was conducted on subjects screened for endogenous hypercortisolism at Amsterdam UMC between December 2015 and February 2022. 25 subjects with and 430 subjects without neoplastic hypercortisolism were included. The diagnosis neoplastic hypercortisolism was confirmed by histology and postoperative clinical features, not on biochemical tests, and excluded based on a follow-up of at least 12 months without progressive clinical signs of neoplastic hypercortisolism. Salivary samples were collected between 22:00–23:59 h and Cortisol_LNSa and Cortisone_LNSa concentrations were measured using liquid chromatography-tandem mass spectrometry.</div></div><div><h3>Results</h3><div>The median Cortisol_LNSa was 7.8 nmol/L for subjects with and 0.8 nmol/L for subjects without neoplastic hypercortisolism (p &lt; 0.001). The median Cortisone_LNSa was 35.5 nmol/L and 5.3 nmol/L, respectively (p &lt; 0.001). Optimal diagnostic accuracy was established at 2.25 nmol/L for Cortisol_LNSa (sensitivity 92 %, specificity 89 %, positive predictive value 32.9 %, negative predictive value 99.5 %) and 15.5 nmol/L for Cortisone_LNSa (sensitivity 93.8 %, specificity 94.5 %, positive predictive value 46.9 %, negative predictive value 99.7 %).</div></div><div><h3>Conclusions</h3><div>We established cut-off values for Cortisol_LNSa and Cortisone_LNSa for the diagnosis of neoplastic hypercortisolism. Cortisone_LNSa provided the highest diagnostic accuracy and we therefore recommend it as the preferred salivary measurement.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120310"},"PeriodicalIF":3.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated CD4− CD8− double-negative T cells in the peripheral blood of SFTS patients are associated with disease severity","authors":"Pan-pan Qiu ,&nbsp;Shi-jie Cai ,&nbsp;Li-li Guo ,&nbsp;Wei Hu ,&nbsp;Hong-yan Song ,&nbsp;Tai-hong Huang ,&nbsp;Zhi-ye Xu ,&nbsp;Sen Wang","doi":"10.1016/j.cca.2025.120312","DOIUrl":"10.1016/j.cca.2025.120312","url":null,"abstract":"<div><h3>Background</h3><div>Severe fever with thrombocytopenia syndrome (SFTS), caused by the Dabie bandavirus, is a fatal infectious disease with no specific vaccines or antiviral treatments. Early diagnosis and severity assessment are critical for clinical management.</div></div><div><h3>Methods</h3><div>This study analyzed peripheral blood samples from 81 SFTS patients and healthy controls, focusing on the distribution of CD4⁺, CD8⁺, CD4⁺CD8⁺ double-positive T (DPT) cells, and CD4⁻CD8⁻ double-negative T (DNT) cells. The relationship between T cell subsets, clinical parameters, viral load, and cytokine levels was examined for diagnostic and prognostic potential.</div></div><div><h3>Results</h3><div>DPT cells were lower and DNT cells higher in SFTS patients compared to controls. DNT cell proportions were higher in severe and fatal cases, correlating positively with viral load, APTT, TT, D-dimer, ferritin, and LDH, and negatively with platelet count, HDL, and fibrinogen. DNT cells were also positively correlated with IFN-α and IFN-γ levels, and higher in patients with complications like shock and sepsis. Furthermore, Cox regression analysis revealed that an elevated DNT cell proportion was an independent risk factor for poor prognosis in SFTS patients.</div></div><div><h3>Conclusion</h3><div>DNT cell proportion changes are linked to SFTS severity and prognosis, suggesting DNT cells as potential biomarkers for early diagnosis and prognosis assessment.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120312"},"PeriodicalIF":3.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haptoglobin phenotype: A germline risk factor for malignant pleural mesothelioma? A case-control study","authors":"Kevin Lamote ,&nbsp;Sigurd Delanghe ,&nbsp;Marijn M. Speeckaert ,&nbsp;Jan P. van Meerbeeck ,&nbsp;Joris R. Delanghe","doi":"10.1016/j.cca.2025.120309","DOIUrl":"10.1016/j.cca.2025.120309","url":null,"abstract":"<div><h3>Purpose</h3><div>The pathogenesis of malignant pleural mesothelioma (MPM) is linked to asbestos-induced chronic inflammation, oxidant formation, hemolysis and subsequent hemoglobin (Hb) release, potentiating oxidative injury. Haptoglobin (Hp) serves as a major antioxidant by binding free Hb in order to prevent its harmful effects. Dependent on the Hp-phenotype, this complexing can be divergent, leading to additional formation of reactive oxygen species (ROS) above those directly induced by asbestos or released by inflammatory cells. In order to determine the Hp-phenotype as a risk factor in MPM, this case-control study compared the Hp-phenotype distribution in MPM patients with asymptomatic persons with former occupational asbestos exposure (AEx) and controls from a European population.</div></div><div><h3>Materials and Methods</h3><div>Hp-phenotyping was done on serum samples of 118 MPM patients and 96 AEx subjects by starch gel electrophoresis. The frequencies of Hp phenotypes (Hp 1–1, Hp 2–1 and Hp 2–2) and alleles (<em>Hp¹</em>, <em>Hp²</em>) were compared with those from 918 healthy control subjects.</div></div><div><h3>Results</h3><div>The Hp 1–1 phenotype was overrepresented in MPM patients compared to AEx persons (P = 0.001) and healthy controls (P = 0.005). The relative risk for developing MPM when having the Hp 1–1 phenotype was 3.05 (1.47–6.34) for AEx subjects and 1.74 (1.19–2.54) for healthy controls compared to other phenotypes.</div></div><div><h3>Conclusion</h3><div>Our results indicate an important role of the Hp-phenotype in MPM pathogenesis suggesting that Hp 1–1 phenotypic persons are more prone for MPM development. Apart from the asbestos-induced radical formation, this finding confirms the role of oxidative stress in cancer development.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120309"},"PeriodicalIF":3.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-educated platelets in lung cancer","authors":"Md Sadique Hussain ,&nbsp;Ehssan Moglad ,&nbsp;Ahsas Goyal ,&nbsp;M.M. Rekha ,&nbsp;Girish Chandra Sharma ,&nbsp;Karthikeyan Jayabalan ,&nbsp;Samir Sahoo ,&nbsp;Anita Devi ,&nbsp;Kavita Goyal ,&nbsp;Gaurav Gupta ,&nbsp;Moyad Shahwan ,&nbsp;Sami I. Alzarea ,&nbsp;Imran Kazmi","doi":"10.1016/j.cca.2025.120307","DOIUrl":"10.1016/j.cca.2025.120307","url":null,"abstract":"<div><div>Non-invasive diagnostic monitoring techniques have become essential for treating lung cancer (LC), which continues to be the primary cause of cancer-related death worldwide. The new diagnostic biomarkers called tumour-educated platelets (TEPs) show strong prospects for providing vital information about tumor biology, tumor spread pathways, and treatment reaction patterns. Despite lacking a nucleus, platelets exhibit an active RNA profile that develops through interactions with tumor-derived compounds and the tumor microenvironments (TME). This review explains platelet-tumour interaction regulatory mechanisms while focusing on platelet contributions toward cancer development, immune system avoidance, and blood clot formation. The detection and classification of LC show promise through the analysis of RNA molecules extracted from platelets that encompass mRNAs and non-coding RNAs. RNA sequencing technology based on TEP demonstrates excellent diagnostic power by correctly identifying LC patients alongside their oncogenic alterations of EGFR, KRAS, and ALK. Treatment predictions have proven successful using platelet RNA profiles, specifically in immunotherapy and targeted therapy. Integrating next-generation sequencing with machine learning and artificial intelligence enhances TEP-based diagnostic tools, improving detection accuracy. Standardizing platelet extraction methods and vesicle purification from tumor material needs better development for effective and affordable clinical use. Future investigations should combine TEPs with circulating tumor DNA and exosomal RNA markers to enhance both earliest-stage LC diagnosis and patient-specific therapeutic approaches. TEPs introduce a groundbreaking technique in oncology since they can transform non-invasive medical diagnostics and therapeutic monitoring for cancer.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120307"},"PeriodicalIF":3.2,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNAs in acute kidney injury","authors":"Mingkang Zhang ,&nbsp;Yazhi Wang ,&nbsp;Yan Zhou ,&nbsp;Xiujuan Wang ,&nbsp;Xin’an Wu","doi":"10.1016/j.cca.2025.120301","DOIUrl":"10.1016/j.cca.2025.120301","url":null,"abstract":"<div><div>Acute kidney injury (AKI) is a clinical syndrome with high morbidity and mortality. AKI has emerged as a significant global public health issue, particularly among hospitalized patients, with the highest incidence observed in those admitted to intensive care units (ICUs). However, early diagnosis of AKI remains challenging due to the limited sensitivity and specificity of conventional biomarkers, including serum creatinine and urine output. Recently, microRNAs (miRNAs) have garnered increasing interest for their potential in the early detection and management of AKI. Owing to their high stability, ease of quantification, well-characterized regulatory functions, and close association with key pathophysiological processes, miRNAs are considered promising diagnostic and therapeutic candidates. Nevertheless, the clinical utility of miRNAs remains limited by confounding factors such as co-infections, comorbidities, and medication use, which may lead to false-positive results. Challenges also persist regarding off-target effects and developing safe and efficient delivery systems. Furthermore, only a few studies have systematically characterized miRNA expression profiles in AKI, considering its heterogeneous etiologies and the dynamic nature of miRNA regulation. Interactions between miRNAs and between miRNAs and non-coding RNAs such as circular (circRNAs) and long non-coding RNAs (lncRNAs) warrant further investigation.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120301"},"PeriodicalIF":3.2,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a rapid immunochromatographic diagnostic test for heparin-induced thrombocytopenia: validation of delayed test and whole blood test","authors":"Paul Billoir ,&nbsp;Virginie Barbay ,&nbsp;Marielle Fresel ,&nbsp;Sabine Brunel ,&nbsp;Véronique Le Cam Duchez","doi":"10.1016/j.cca.2025.120291","DOIUrl":"10.1016/j.cca.2025.120291","url":null,"abstract":"<div><div>Heparin-induced thrombocytopenia (HIT) is a rare, iatrogenic condition, characterized by its potential severity and diagnostic difficulties. There are rapid diagnostic tests for suspected HIT. Among these, the STic Expert® HIT has a good sensitivity to detect HIT. However, it must be performed within 2 h after sampling. The aim of this study was to evaluate a delayed STic Expert® HIT test in plasma and whole blood.</div><div>Thirty-six patients were prospectively included for HIT testing delayed analysis and 64 for whole blood analysis. For any request for HIT testing, an analysis by STic Expert® HIT was performed within 2 h and 8 h post-sampling. Any positive result was confirmed by a functional test, platelet aggregation with heparin, release of ¹⁴C-serotonin assay (SRA), and immunological assay for anti-platelet factor 4 IgG antibodies.</div><div>Among the 36 patients for delayed analysis, 20 had an initially positive STic Expert® HIT test. Among the 64 patients for whole blood analysis, 20 had an initially positive STic Expert® HIT test, and 24 positives in whole blood. For the test performed at T0, Se = 100 %, Sp = 68.4 %, PPV = 73.9 %, and NPV = 100 %. The Chi-square (X²) = 18.2 with p &lt; 0.001.</div><div>For the test performed at T = 8 h, Se = 100 %, Sp = 68.4 %, PPV = 73.9 %, and NPV = 100 %. The X² = 18.2 with p &lt; 0.001. For the test performed in whole blood, Se = 100 %, Sp = 74.4 %, PPV = 37.5 %, and NPV = 100 %. The X² = 18.6 with p &lt; 0.001.</div><div>In conclusion, we have demonstrated that the STic Expert® can be used to perform an HIT diagnostic test 8 h after sampling and on whole blood. However, this study needs to be confirmed on a larger number of samples.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120291"},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"suPAR as a biomarker of support in different clinical settings","authors":"Fabio Belvederi ,&nbsp;Simone Leggeri ,&nbsp;Andrea Urbani ,&nbsp;Silvia Baroni","doi":"10.1016/j.cca.2025.120303","DOIUrl":"10.1016/j.cca.2025.120303","url":null,"abstract":"<div><div>The urokinase-type plasminogen activator receptor (uPAR) system, which includes protease, receptor and inhibitors, is essential for key cellular functions like immune activation, cell migration, and tissue remodeling. Soluble uPAR (suPAR), released into circulation, serves as a valuable biomarker for systemic inflammation and immune activation. Elevated suPAR levels are associated with disease severity in conditions such as infections, sepsis, cardiovascular diseases, renal injury, cancer, and autoimmune diseases providing prognostic value especially in acute settings.</div><div>Recent advancements in diagnostic methods, have enhanced the accuracy of suPAR measurement in serum and plasma. New rapid tests, such as suPARnostic Quick Triage, as well as turbidimetric assays, further expand its clinical applicability.</div><div>In this review, we discuss the suPAR biomarker, focusing on its biochemical structure, biological functions, measurement methods and areas of clinical interest in different fields of medicine.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120303"},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNAs as biomarkers in brain metastasis","authors":"Farag M.A. Altalbawy ,&nbsp;Ahmed Hussein Zwamel ,&nbsp;Gaurav Sanghvi ,&nbsp;Roopashree R ,&nbsp;Mukesh Kumari ,&nbsp;Aditya Kashyap ,&nbsp;S. Gayathri ,&nbsp;Rajashree Panigrahi ,&nbsp;Aziza Makhmudova ,&nbsp;Safia Obaidur Rab","doi":"10.1016/j.cca.2025.120292","DOIUrl":"10.1016/j.cca.2025.120292","url":null,"abstract":"<div><div>Cancer patients face a particularly daunting obstacle when tumors spread to the brain, a condition that substantially increases mortality rates. Traditional diagnostic tools have proven inadequate, creating an urgent need for less invasive detection methods. Among emerging solutions, noncoding RNAs (ncRNAs), particularly microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), have captured researchers’ attention. These molecular elements play key roles in determining disease outcomes and treatment response in brain metastases, helping scientists better understand disease mechanisms and identify potential therapeutic interventions. Research has revealed altered patterns of ncRNA expression across various primary cancers that spread to the brain, suggesting new possibilities for treatment and prevention strategies. By examining ncRNA patterns in blood serum and cerebrospinal fluid, clinicians can potentially distinguish brain metastases from primary brain tumors without invasive procedures. The immune response within the brain microenvironment is notably influenced by ncRNAs, with miRNAs playing an especially crucial role. miRNAs show particular promise as diagnostic markers, helping to separate healthy from cancerous tissue and determine the original source of brain metastases. The therapeutic potential of miRNAs is equally significant, as targeting miRNAs could lead to more effective treatments with fewer side effects. Given the current scarcity of treatment options for brain metastases, the use of ncRNAs, especially miRNAs, represents a promising development in both diagnosis and treatment. Additional clinical research is needed to confirm the accuracy and reliability of ncRNA-based approaches, which could revolutionize how healthcare providers address this challenging aspect of cancer care and improve patient outcomes.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120292"},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of trimethylamine-N-oxide (TMAO) and its main related trimethylammonium-containing compounds in human plasma by LC-MS/MS","authors":"Salvatore Sotgia","doi":"10.1016/j.cca.2025.120294","DOIUrl":"10.1016/j.cca.2025.120294","url":null,"abstract":"<div><h3>Background</h3><div>Trimethylammonium-containing compounds, including choline (CHOL), carnitine (CAR), trimethylglycine (TMG), ergothioneine (ERT), Nε,Nε,Nε-trimethyllysine (TML), γ-butyrobetaine (gBB), and dimethylglycine (DMG) contribute to trimethylamine N-oxide (TMAO) production, a metabolite linked to cardiovascular, renal, and metabolic diseases. An LC-MS/MS method has been established for their simultaneous measurement in human plasma, as an accurate quantification of TMAO and its precursors is crucial for understanding its clinical relevance.</div></div><div><h3>Methods</h3><div>Blood samples from ten healthy male volunteers were processed using acetonitrile protein precipitation. Analysis was performed using a HILIC column and an isocratic methanol-formic acid mobile phase, achieving a total run time of less than 6 min. Linearity was adequate for all analytes (R² &gt; 0.995), with mean intra- and inter-assay variation coefficients of 2.88 % and 4.23 %, respectively. Recoveries ranged from 95 % to 101 %, limits of detection from 0.009 to 0.068 µmol/L, and limits of quantification from 0.031 to 0.187 µmol/L. Plasma mean concentrations were 3.18 ± 0.73 µmol/L for TMAO, 3.99 ± 0.65 µmol/L for DMG, 9.84 ± 2.08 µmol/L for CHOL, 24.22 ± 6.19 µmol/L for TMG, 0.54 ± 0.22 µmol/L for gBB, 57.29 ± 8.89 µmol/L for CAR, 1.10 ± 0.42 µmol/L for ERT, and 0.40 ± 0.11 µmol/L for TML. Significant inter-individual variability (mean RSD% of 26 %) was observed.</div></div><div><h3>Conclusion</h3><div>The developed LC-MS/MS method enables rapid, sensitive, and selective quantification of TMAO and its precursors in human plasma. The analytical performance supports its application in clinical and metabolomic studies, contributing to a better understanding the role of TMAO in disease states.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"573 ","pages":"Article 120294"},"PeriodicalIF":3.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}