Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih最新文献_第4页

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2013-06-01

引用次数: 0

Cardiac electrophysiological differences between Kunming and C57BL6/J mice. 昆明小鼠与C57BL6/J小鼠心脏电生理差异。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Teng Wang, Mu Qin, He Huang, Hong-liang Li, Cong-xin Huang

{"title":"Cardiac electrophysiological differences between Kunming and C57BL6/J mice.","authors":"Teng Wang, Mu Qin, He Huang, Hong-liang Li, Cong-xin Huang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the alterations of cardiac electrophysiological properties and substantial mechanism and find the stable arrhythmia mouse model in Kunming (KM) and C57BL6/J (C57) mice.Methods: Electrocardiogram recordings were used to analyze the QT interval in vivo, and mono- phasic action potential of right and left ventricular epicardium was recorded to elicit changes of action potential duration (APD) in conventional and programmed electrical stimulation (PES). Transient outward potassium current (Ito) was recorded via whole-cell patch-clamp technique in single right and left epicardial myocytes.Results: QT interval was prolonged in KM mice relative to C57 mice (62.51±4.47 ms vs. 52.59±4.85 ms, P<0.05) The APD at 50% repolarization of the left ventricular epicardium (18.60±0.91 ms vs. 12.90±0.35 ms), and APDs at 50% (17.31±6.05 ms vs. 12.00±3.24 ms) and 70% repolarization (36.13±5.32 ms vs. 21.95±8.06 ms) of the right ventricular epicardium in KM mice were more sensitive to PES-induced ventricular tachycardia (25%, 3 of 12 hearts), and especially to Burst-induced ventricular tachycardia (50%, 6 of 12 hearts)compared with C57 mice, which were 20% (2 of 10 hearts) and 30% (3 of 10 hearts) respectively. Ito densities both in the left and right ventricular epicardial myocytes from KM mice were significantly decreased compared with C57 mice, respectively (all P<0.01).Conclusion: Our data showed that KM mice with the prolonged QT interval and APD are vulnerabilities to ventricular arrhythmia, which are attributed to lower Ito densities in ventricular myocytes obtained from KM mice than that from C57 mice.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"27 2","pages":"80-7"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30743712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary meningeal melanocytoma located in foramen magnum: a case report and review of the literatures. 位于枕骨大孔的原发性脑膜黑素细胞瘤1例报告及文献复习。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Ming-chao Fan, Jing-feng Wang, Wei-wei Fu, Ke Liu, Lian-di Li, Peng Sun

引用次数: 0

Gastric metastasis of atypical medullary carcinoma from breast: a case report. 乳腺非典型髓样癌胃转移1例。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Bo Jin, Yan-ping Liu, Hai-jiang Wang, Qi-shan Wang

引用次数: 0

Fresh frozen plasma for the treatment of hereditary angioedema acute attacks. 新鲜冷冻血浆治疗遗传性血管性水肿急性发作。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Rui Tang, Shi Chen, Hong-yu Zhang

{"title":"Fresh frozen plasma for the treatment of hereditary angioedema acute attacks.","authors":"Rui Tang, Shi Chen, Hong-yu Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine the safety and efficacy of fresh frozen plasma (FFP) infusion for the treatment of hereditary angioedema (HAE).Methods: The medical records of patients with HAE admitted to Peking Union Medical College Hospital who had received FFP infusion during 2004 and 2010 were reviewed and PubMed database from 1966 to the present were searched using the following hereditary angioedema and fresh frozen plasma. The patient's age, sex, body location of HAE attacks, the dose of FFP infusion, time of beginning to improvement, time to complete remission, complication, C1 inhibitor activity, and outcome were analyzed.Results: A total of 13 enrolled patients (7 male and 6 female) received 16 times of FFP infusion, including 2 patients undergoing FFP infusion in Peking Union Medical College Hospital and 11 patients reported in the literature. The mean dosage of FFP infusion was 586∓337 mL. Two cases suffered from worsening abdominal pain and one case experienced skin rash. Only 1 patient had no improvement in symptom owing to transfusion related reaction. There was a definite improvement in symptom 49∓19 minutes after beginning FFP infusion. The remission time decreased from 61.7∓27.0 hours to 3.3 (2.0, 12.0) hours after FFP infusion. FFP infusion was effective for both type I and type II HAE.Conclusion: FFP seems to be safe and effective for acute attacks of HAE.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"27 2","pages":"92-5"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30743714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of ADAM10 and ADAM17 in CD16b shedding mediated by different stimulators. ADAM10和ADAM17在不同刺激物介导的CD16b脱落中的作用。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Sha Guo, Min Peng, Qing Zhao, Wei Zhang

{"title":"Role of ADAM10 and ADAM17 in CD16b shedding mediated by different stimulators.","authors":"Sha Guo, Min Peng, Qing Zhao, Wei Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the main proteinases responsible for CD16b shedding under different stimulators.Methods: HEK293 cell line stably expressing CD16b was constructed by lentivirus system. The cell line was then overexpressed with a disintegrin and metalloproteinase 10 (ADAM10) or ADAM17, suppressed with short hairpin RNA of ADAM10 or ADAM17, and reconstituted with ADAM10 or ADAM17, respectively. After each treatment, the cell line was stimulated with ionomycin or phorbol 12-myristate- 13-acetate (PMA) for 12 hours. The soluble CD16b released from cell membrane was detected by immunoprecipition and immunoblot. Quantitation was then implemented to compare the amount of soluble CD16b in cell supernatant after stimulation.Results: HEK293 cell line stably expressing CD16b was successfully established. When CD16b expressing cell line was overexpressed with ADAM10, shedding of CD16b was increased after stimulation with ionomycin but not PMA; when the cell line overexpressed with ADAM17, shedding of CD16b was increased after stimulation with PMA but not ionomycin. Similarly, when ADAM10 was suppressed by short hairpin RNA, CD16b shedding was decreased after stimulation with ionomycin; when ADAM17 was suppressed by short hairpin RNA, CD16b shedding was decreased after stimulation with PMA. The shedding of CD16b was increased again when CD16b expressing cell line was reconstituted with ADAM10 and stimulated by ionomycin or reconstituted with ADAM17 and stimulated by PMA.Conclusions: Both ADAM10 and ADAM17 could shed CD16b, but they possess differed preferences. ADAM10 is the main sheddase under stimulation of ionomycin, while ADAM17 is the main sheddase under stimulation of PMA.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"27 2","pages":"73-9"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30743711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilateral ureteral fibroepithelial polyps: a case report. 双侧输尿管纤维上皮息肉1例。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Ji-rui Niu, Shi Rong, Zhi-gang Ji, Hua Fan, Jing-min Zhou, Zhen-yu Zhang

引用次数: 0

Correlations between serum uric acid level and disease activity, intrathecal inflammation reactivity in patients with multiple sclerosis. 多发性硬化症患者血清尿酸水平与疾病活动度、鞘内炎症反应性的相关性

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01 DOI: 10.1007/BF03378998

Cai-yan Liu, Yan Xu, Li-ying Cui, Bin Peng, Li-zhen Zhong, Xing-wang Chen, Jian-ming Wang

{"title":"Correlations between serum uric acid level and disease activity, intrathecal inflammation reactivity in patients with multiple sclerosis.","authors":"Cai-yan Liu, Yan Xu, Li-ying Cui, Bin Peng, Li-zhen Zhong, Xing-wang Chen, Jian-ming Wang","doi":"10.1007/BF03378998","DOIUrl":"https://doi.org/10.1007/BF03378998","url":null,"abstract":"Objective: To explore the correlations between serum uric acid (UA) levels and the clinical and cerebrospinal fluid (CSF) parameters of multiple sclerosis (MS).Methods: The medical reports of 47 MS patients admitted to Peking Union Medical College Hospital during 2008 and 2010 were reviewed. And 49 age- and gender-matched cerebral infarction patients were enrolled as control. The mean serum UA level of the MS patients was compared with that of the control group. The correlations between the UA levels and the clinical parameters including gender, disease duration, relapse rate, and disease disabilities as assessed by the Expanded Disability Status Scale score, were explored. Forty-one patients had CSF examinations. The correlations between the UA levels and the CSF parameters reflecting inflammation and tissue damage, including CSF protein, white blood cell count, oligoclonal band, 24-hour IgG index, and myelin basic protein, were also investigated.Results: The mean serum UA level in the MS patients was lower than that in the control group (247.75±52.59 µmol/L vs. 277.94±74.33 µmol/L, P=0.025) and inversely correlated with the relapse rate (P=0.049). MS patients with lower serum UA levels tended to have higher white blood cell counts and myelin basic protein level. But there was no correlation between CSF protein levels (r=0.165, P=0.273), white blood cell counts (r=-0.051, P=0.732), IgG index (r=0. 045, P=0.802), or myelin basic protein level (r=-0.248, P=0.145) and the serum UA level, respectively.Conclusion: In MS patients, UA levels might partly reflect the extent of disability and inflammation.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"27 2","pages":"88-91"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF03378998","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30743713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hemi-semi laminectomy approach for the microsurgical treatment of spinal schwannomas. 半-半椎板切除术在脊柱神经鞘瘤显微外科治疗中的应用。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Xing Su, Wei Shi, Qing-feng Huang, Jian-hong Shen, Jian Chen

引用次数: 0

Function of microRNA-346 and its roles in human diseases. microRNA-346的功能及其在人类疾病中的作用

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2012-06-01

Jin-hua He, Yu-guang Li

引用次数: 0