Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih最新文献_第10页

Application value of magnetic resonance sequences in diagnosis of early spinal metastatic tumor. 磁共振序列在早期脊柱转移瘤诊断中的应用价值。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Li-Xia Wang, Xiang-Quan Kong, He-Shui Shi, Ding-Xi Liu, Yin Xiong

{"title":"Application value of magnetic resonance sequences in diagnosis of early spinal metastatic tumor.","authors":"Li-Xia Wang, Xiang-Quan Kong, He-Shui Shi, Ding-Xi Liu, Yin Xiong","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the clinical value of different magnetic resonance (MR) pulse sequences in diagnosis of spinal metastatic tumor.Methods: Fifteen patients with clinically suspected spinal metastatic tumor were included in this study. These patients were with documented primary tumors. Four MR pulse sequences, T1-weighted spin echo (T1WI SE), T2-weighted fast spin echo (T2WI FSE), short time inversion recovery (STIR), and gradient echo 2-D multi echo data imaging combination (GE Me-2D) were used to detect spinal metastasis.Results: Fifteen vertebral bodies were entire involvement, 38 vertebral bodies were section involvement, and totally 53 vertebral bodies were involved. There were 19 focal infections in pedicle of vertebral arch, 15 metastases in spinous process and transverse process. Fifty-three vertebral bodies were abnormal in T1 WI SE and GE Me-2D, 35 vertebral bodies were found abnormal in T2WI FSE, and 50 vertebral bodies were found abnormal in STIR. The verges of focal signal of involved vertebral bodies were comparatively clear in T1WI SE, comparatively clear or vague in T2WI FSE, vague in STIR, and clear in GE Me-2D.Conclusions: GE Me-2D may be the most sensitive technique to detect metastases. So three sequences (T1WI SE, T2WI FSE, GE Me-2D) can demonstrate the early changes of spinal metastasis roundly.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"9-12"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ureteral injury during gynecological laparoscopic surgeries: report of twelve cases. 妇科腹腔镜手术输尿管损伤12例报告。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Jin-Song Gao, Jin-Hua Leng, Zhu-Feng Liu, Keng Shen, Jing-He Lang

{"title":"Ureteral injury during gynecological laparoscopic surgeries: report of twelve cases.","authors":"Jin-Song Gao, Jin-Hua Leng, Zhu-Feng Liu, Keng Shen, Jing-He Lang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate ureteral injury during gynecological laparoscopic surgeries.Methods: From January 1990 to December 2005, 12 868 gynecological laparoscopic surgeries were conducted in Peking Union Medical College Hospital with 12 ureteral injuries reported. The present study investigated several aspects, including surgical indications, uterine size, pelvic adhesion, operative procedures, symptoms, diagnostic time and methods, injury site and type, subsequent treatment, and prognosis.Results: The incidence of ureteral injury was 0.093% (12/12 868) in all cases, 0.42% (11/2 586) in laparoscopic hysterectomy [laparoscopically assisted vaginal hysterectomy (LAVH) or total laparoscopic hysterectomy (TLH)], and 0.01% (1/10 282) in non-LAVH surgeries. Enlarged uterus, pelvic adhesion, and endometrosis were risk factors associated with ureteral injury. Only one injury was found intraoperatively while others were found postoperatively. The injury sites were at the pelvic brim (2 cases) or the lower part of ureter (10 cases). Patients were treated with ureteral stenting (effective in 2 cases) or laparotomy and open repair. Prognoses were favorable in most cases.Conclusions: Most laparoscopic ureteral injuries occur during laparoscopic hysterectomy. Further evaluation is required when ureteral injury is suspected, and surgical repair is the major treatment for ureteral injury.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"13-6"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mediastinal cavernous hemangioma: report of one case. 纵隔海绵状血管瘤附1例报告。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Xue-Zhong Xing, Wen-Dong Lei, De-Chao Zhang, Jie He

引用次数: 0

Functional and structural recovery of injured spinal cord following delayed X-irradiation in rats. 延迟x射线照射损伤大鼠脊髓的功能和结构恢复。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Gang Li, Xin-Gang Li, De-Ze Jia, Dong-Hai Wang, Yu-Hang Su, Qing-Lin Zhang

{"title":"Functional and structural recovery of injured spinal cord following delayed X-irradiation in rats.","authors":"Gang Li, Xin-Gang Li, De-Ze Jia, Dong-Hai Wang, Yu-Hang Su, Qing-Lin Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats.Methods: Seventy Sprague-Dawley rats were randomly divided into two groups, 35 rats in each. The control group sustained a one-minute clip compression (force of clip was 30 g) injury of the spinal cord at the T2 level, without X-irradiation. The experimental group received X-irradiation 14 days after injury. Neurological function was assessed by the modified Tarlov method, including hind limbs movement, inclined plane, and pain withdrawal. These tests were performed in a blinded fashion at 3, 7, 14, 21, 28, 35, and 42 days after injury. At 43 days after injury, histological examination of the injured spinal cord was performed following decapitation of the rats.Results: Sixty-two rats met the experimental requirements (spinal cord injury was similar), 32 rats in experimental group and 30 rats in control group. Statistically significant difference was observed between the two groups in hind limbs movement and inclined plane (P < 0.01), but not in the pain withdrawal test. The edema and necrosis areas of injured spinal cords in experimental group were less than those in control group, and axons in experimental group were significantly more than those in control group (P < 0.01).Conclusion: Delayed X-irradiation following spinal cord injury may enhance functional recovery by improving and restoring structural integrity of the injured spinal cord in rats.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"58-61"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of short- and long-term outcomes between Cypher and TAXUS drug-eluting stents for in-stent restenosis. Cypher和TAXUS药物洗脱支架治疗支架内再狭窄的短期和长期疗效比较。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Ji-Lin Chen, Yue-Jin Yang, Shu-Bin Qiao, Min Yao, Xue-Wen Qin, Bo Xu, Hai-Bo Liu, Yong-Jian Wu, Jin-Qing Yuan, Jue Chen, Shi-Jie You, Jun Dai, Jian-Jun Li, Run-Lin Gao

{"title":"Comparison of short- and long-term outcomes between Cypher and TAXUS drug-eluting stents for in-stent restenosis.","authors":"Ji-Lin Chen, Yue-Jin Yang, Shu-Bin Qiao, Min Yao, Xue-Wen Qin, Bo Xu, Hai-Bo Liu, Yong-Jian Wu, Jin-Qing Yuan, Jue Chen, Shi-Jie You, Jun Dai, Jian-Jun Li, Run-Lin Gao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To compare the short- and long-term clinical outcomes between sirolimus-eluting stent (Cypher stent) and paclitaxel-eluting stent (TAXUS stent) in patients with in-stent restenosis (ISR) lesions of the coronary arteries.Methods: From December 2002 to March 2005, 253 patients with ISR lesions of the coronary arteries were selected and divided into two groups. Cypher group (152 cases) was treated with Cypher or Cypher Select stents, and TAXUS group (101 cases) with TAXUS stents. A total of 262 ISR lesions in these patients were treated with 308 drug-eluting stents (DESs), including 176 Cypher or Cypher Select stents and 132 TAXUS stents. All patients were followed up for 10 months. Procedure success rates of DES implantation in both groups were observed. Major adverse cardiac events (MACE) rates in hospital and at 10 months follow-up, as well as in-DES restenosis observed using coronary angiography at follow-up were compared between two groups.Results: Success rate of DES implantation was 100% in both groups. No significant difference in MACE rate during hospitalization was found between the two groups. However, at 10 months follow-up, MACE rate was higher in TAXUS group than in Cypher group (16.00% vs. 6.67% , P = 0.031). As for coronary angiography at 10 months follow-up, we observed an increasing tendency of in-DES restenosis rate in TAXUS group compared with Cypher group (29.41% vs. 14.04%, P = 0.075).Conclusions: Cypher and TAXUS DESs both have good short- and long-term outcomes in treating ISR. Cypher DES proved better long-term clinical outcome than TAXUS DES.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of p38 mitogen-activated protein kinase in E. coli-induced U937 apoptosis. p38丝裂原活化蛋白激酶参与大肠杆菌诱导的U937细胞凋亡。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Jia-He Wang, Yi-Jun Zhou, Ping He, Bai-Yi Chen

引用次数: 0

Plasma resistin levels and single-nucleotide polymorphisms in resistin gene 5' flanking region in patients with stroke. 脑卒中患者血浆抵抗素水平及抵抗素基因5′侧区单核苷酸多态性。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Xing-Jian Lin, Ying-Dong Zhang, Qing-Shan Guan, Qing Di, Jing-Ping Shi, Wei-Guo Liu

{"title":"Plasma resistin levels and single-nucleotide polymorphisms in resistin gene 5' flanking region in patients with stroke.","authors":"Xing-Jian Lin, Ying-Dong Zhang, Qing-Shan Guan, Qing Di, Jing-Ping Shi, Wei-Guo Liu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To analyze the role of resistin in insulin resistance (IR) through investigating the variation of plasma resistin levels and single-nucleotide polymorphisms (SNPs) in resistin gene 5' flanking region in stroke patients.Methods: In 103 atherothrombotic cerebral infarction (ACI) patients, 85 lacunar infarction (LI) patients, 70 intracerebral hemorrhage (ICH) patients, and 86 healthy controls, plasma resistin and insulin levels were measured by ELISA, SNPs in resistin gene 5' flanking region were detected by PCR and direct DNA sequencing. The subjects' body height and weight, the body mass index, quantitative insulin sensitivity check index (QUICKI), blood pressure, and the concentration of fasting plasma glucose, triglyceride, total cholesterol, creatinine, low-density lipoprotein, and high-density lipoprotein were also determined.Results: QUICKI was significantly lower in the ACI and ICH patients (0.316 +/- 0.037 and 0.309 +/- 0.032, respectively) than that in the controls (0.342 +/- 0.043, P < 0.001), while plasma resistin level was significantly higher in the ACI and ICH patients (6.36 +/- 3.79 and 7.15 +/- 4.27 ng/mL, respectively) than that in the controls (5.28 +/- 2.56 ng/mL, P < 0.05), but such difference was not observed in the LI patients compared with controls. There was a statistically negative correlation between plasma resistin level with QUICKI (r = -0.228, P < 0.001). The distributions of allele and genotype frequencies of resistin gene - 420C > G and - 537A > C SNPs were not significantly different among the different groups, and those SNPs were not correlated with other clinical and biochemical parameters.Conclusions: Plasma resistin is associated with stroke by participating in the development of IR. The SNPs in resistin gene 5' flanking region has no impact on the plasma resistin level.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"27-32"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of gamma-interferon with TRAIL and cisplatin or etoposide induces apoptosis in human neuroblastoma cell line SH-SY5Y. γ -干扰素联合TRAIL和顺铂或依托泊苷诱导人神经母细胞瘤SH-SY5Y细胞凋亡。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Hai-Xia Tong, Chun-Wei Lu, Ji-Hong Zhang, Li Ma, Jin-Hua Zhang

{"title":"Combination of gamma-interferon with TRAIL and cisplatin or etoposide induces apoptosis in human neuroblastoma cell line SH-SY5Y.","authors":"Hai-Xia Tong, Chun-Wei Lu, Ji-Hong Zhang, Li Ma, Jin-Hua Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the effect of gamma-interferon (IFNgamma), tumor necrosis factor related apoptosis inducing ligand (TRAIL), and cisplatin or etoposide induced apoptosis in human neuroblastoma cell line SH-SY5Y and its possible molecular mechanisms.Methods: The expressions of Caspase 8 mRNA and protein were detected with RT-PCR and Western blot analysis. The effects of IFN-gamma, TRAIL, IFNgamma + TRAIL, IFN-gamma + Caspase 8 inhibitor + TRAIL, IFNgamma + cisplatin + TRAIL, and IFNgamma + etoposide + TRAIL on the growth and apoptosis of SH-SY5Y cells were detected with the methods of MTT and flow cytometry. The relative Caspase 8 activity was measured with colorimetric assay.Results: Caspase 8 was undetectable in SH-SY5Y cells but an increased expression of Caspase 8 mRNA and protein was found after treatment with IFNgamma. SH-SY5Y cells themselves were not sensitive to TRAIL, but those expressing Caspase 8 after treatment with IFNgamma were. The killing effect of TRAIL on SH-SY5Y cells expressing Caspase 8 was depressed by Caspase 8 inhibitor. Cisplatin and etoposide could enhance the sensitivity of TRAIL on SH-SY5Y cells. The relative Caspase 8 activity of SH-SY5Y cells in IFN-gamma + TRAIL group was significantly higher than those of control group, IFN-gamma group, TRAIL group, and inhibitor group (P < 0.01). There was no significant difference among IFN-gamma + TRAIL group, IFNgamma + cisplatin + TRAIL group, and IFNgamma + etoposide + TRAIL group.Conclusions: IFNgamma could sensitize SH-SY5Y cells to TRAIL-induced apoptosis and this may be realized by the up-regulation of Caspase 8. Cisplatin and etoposide could enhance the killing effect of TRAIL on SH-SY5Y cells.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"38-43"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of angiotensin II type 1 receptor-associated protein on vascular smooth muscle cell growth and neointimal formation. 血管紧张素II型1受体相关蛋白对血管平滑肌细胞生长和新内膜形成的抑制作用。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Zhen Li, Zhong-Gao Wang, Xiu Chen, Xiao-Dong Chen

{"title":"Inhibitory effect of angiotensin II type 1 receptor-associated protein on vascular smooth muscle cell growth and neointimal formation.","authors":"Zhen Li, Zhong-Gao Wang, Xiu Chen, Xiao-Dong Chen","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the mechanism of a novel angiotensin II type 1 receptor-associated protein (ATRAP) interfering with angiotensin II type 1 (AT1) receptor-mediated vascular smooth muscle cell (VSMC) growth and neointimal formation.Methods: VSMCs isolated from thoracic aorta of adult Sprague-Dawley (SD) rats were used in this study. ATRAP cDNA was subcloned into pcDNA3 vector and then transfected into VSMCs. DNA synthesis and extracellular signal-regulated kinase (ERK) and phospho-ERK expressions in VSMCs were assayed by measurement of 3H thymidine incorporation and Western blotting, respectively. Morphological changes were observed in the balloon injured artery with or without transfection of ATRAP cDNA using 12-week-old male SD rats.Results: ATRAP overexpression in VSMCs inhibited angiotensin II (Ang II)-induced 3H thymidine incorporation 48 hours after Ang II stimulation (P < 0.05). In VSMC, Ang II stimulation increased the phosphorylation of ERK, which reached the peak around 60 minutes. The activation of phospho-ERK was significantly decreased by ATRAP (P < 0.05). Neointimal formation was markedly inhibited by ATRAP overexpression in injuried arteries.Conclusions: The AT1 receptor-derived activation of ERK plays an essential role in Ang II-induced VSMC growth. The growth inhibitory effects of ATRAP might be due to interfering with AT1 receptor-mediated activation of ERK in VSMC growth and neointimal formation.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"22-6"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rna interference of annexin II gene in PC3 cells by using small interference RNA synthesized with in vitro transcription. 体外转录合成小干扰Rna干扰PC3细胞膜联蛋白II基因的研究。

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih Pub Date : 2007-03-01

Ya-Wei Yuan, Ai-Min Sun, Ying Lui, Long-Hua Chen, A G Banerjee

{"title":"Rna interference of annexin II gene in PC3 cells by using small interference RNA synthesized with in vitro transcription.","authors":"Ya-Wei Yuan, Ai-Min Sun, Ying Lui, Long-Hua Chen, A G Banerjee","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To silence annexin II gene expression by using small interference RNA (siRNA) in prostate cancer cell line PC3.Methods: For in vitro transcription, four sequences of 29-nucleotide DNA template oligonucleotides were designed, and one pair of the sequences were complementary to annexin II gene. The other pair was negative control. The 8 nucleotides at the 3' end of each oligonucleotide were complementary to the T7 Promoter Primer. The sense and antisense siRNA templates were transcribed by T7 RNA polymerase and the resulting RNA transcripts were hybridized to create dsRNA. The siRNA was transfected into prostate cancer cell PC3. For assaying the efficiency of siRNA, confocal microscopy, Northern blotting, and Western blotting were employed to examine the expression of annexin II protein and its mRNA. 3H thymidine was used to measure DNA synthesis.Results: The siRNA sequence specific to annexin II gene was capable of inhibiting the expression of annexin II protein and its mRNA. And cellular DNA synthesis was significantly reduced in siRNA transfected cells.Conclusions: The protocol for the synthesis of siRNA by T7 RNA polymerase is feasible. Annexin II might be involved in DNA synthesis.","PeriodicalId":10186,"journal":{"name":"Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih","volume":"22 1","pages":"33-7"},"PeriodicalIF":0.0,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26672412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0