Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2024-09-27DOI: 10.1097/CM9.0000000000003312
Xiaokai Fang, Shan Zhang, Mingyang Wu, Yang Luo, Xingyu Chen, Yuan Zhou, Yu Zhang, Xiaochun Liu, Xu Yao
{"title":"Systemic comparison of molecular characteristics in different skin fibroblast senescent models.","authors":"Xiaokai Fang, Shan Zhang, Mingyang Wu, Yang Luo, Xingyu Chen, Yuan Zhou, Yu Zhang, Xiaochun Liu, Xu Yao","doi":"10.1097/CM9.0000000000003312","DOIUrl":"10.1097/CM9.0000000000003312","url":null,"abstract":"<p><strong>Background: </strong>Senescent human skin primary fibroblast (FB) models have been established for studying aging-related, proliferative, and inflammatory skin diseases. The aim of this study was to compare the transcriptome characteristics of human primary dermal FBs from children and the elderly with four senescence models.</p><p><strong>Methods: </strong>Human skin primary FBs were obtained from healthy children (FB-C) and elderly donors (FB-E). Senescence models were generated by ultraviolet B irradiation (FB-UVB), D-galactose stimulation (FB-D-gal), atazanavir treatment (FB-ATV), and replication exhaustion induction (FB-P30). Flow cytometry, immunofluorescence staining, real-time quantitative polymerase chain reaction, co-culturing with immune cells, and bulk RNA sequencing were used for systematic comparisons of the models.</p><p><strong>Results: </strong>In comparison with FB-C, FB-E showed elevated expression of senescence-related genes related to the skin barrier and extracellular matrix, proinflammatory factors, chemokines, oxidative stress, and complement factors. In comparison with FB-E, FB-UVB and FB-ATV showed higher levels of senescence and expression of the genes related to the senescence-associated secretory phenotype (SASP), and their shaped immune microenvironment highly facilitated the activation of downstream immune cells, including T cells, macrophages, and natural killer cells. FB-P30 was most similar to FB-E in terms of general transcriptome features, such as FB migration and proliferation, and aging-related characteristics. FB-D-gal showed the lowest expression levels of senescence-related genes. In comparisons with the single-cell RNA sequencing results, FB-E showed almost complete simulation of the transcriptional spectrum of FBs in elderly patients with atopic dermatitis, followed by FB-P30 and FB-UVB. FB-E and FB-P30 showed higher similarity with the FBs in keloids.</p><p><strong>Conclusions: </strong>Each senescent FB model exhibited different characteristics. In addition to showing upregulated expression of natural senescence features, FB-UVB and FB-ATV showed high expression levels of senescence-related genes, including those involved in the SASP, and FB-P30 showed the greatest similarity with FB-E. However, D-galactose-stimulated FBs did not clearly present aging characteristics.</p>","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2180-2191"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142342698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development and multicenter validation of machine learning models for predicting postoperative pulmonary complications after neurosurgery.","authors":"Ming Xu, Wenhao Zhu, Siyu Hou, Hongzhi Xu, Jingwen Xia, Liyu Lin, Hao Fu, Mingyu You, Jiafeng Wang, Zhi Xie, Xiaohong Wen, Yingwei Wang","doi":"10.1097/CM9.0000000000003433","DOIUrl":"10.1097/CM9.0000000000003433","url":null,"abstract":"<p><strong>Background: </strong>Postoperative pulmonary complications (PPCs) are major adverse events in neurosurgical patients. This study aimed to develop and validate machine learning models predicting PPCs after neurosurgery.</p><p><strong>Methods: </strong>PPCs were defined according to the European Perioperative Clinical Outcome standards as occurring within 7 postoperative days. Data of cases meeting inclusion/exclusion criteria were extracted from the anesthesia information management system to create three datasets: The development (data of Huashan Hospital, Fudan University from 2018 to 2020), temporal validation (data of Huashan Hospital, Fudan University in 2021) and external validation (data of other three hospitals in 2023) datasets. Machine learning models of six algorithms were trained using either 35 retrievable and plausible features or the 11 features selected by Lasso regression. Temporal validation was conducted for all models and the 11-feature models were also externally validated. Independent risk factors were identified and feature importance in top models was analyzed.</p><p><strong>Results: </strong>PPCs occurred in 712 of 7533 (9.5%), 258 of 2824 (9.1%), and 207 of 2300 (9.0%) patients in the development, temporal validation and external validation datasets, respectively. During cross-validation training, all models except Bayes demonstrated good discrimination with an area under the receiver operating characteristic curve (AUC) of 0.840. In temporal validation of full-feature models, deep neural network (DNN) performed the best with an AUC of 0.835 (95% confidence interval [CI]: 0.805-0.858) and a Brier score of 0.069, followed by Logistic regression (LR), random forest and XGBoost. The 11-feature models performed comparable to full-feature models with very close but statistically significantly lower AUCs, with the top models of DNN and LR in temporal and external validations. An 11-feature nomogram was drawn based on the LR algorithm and it outperformed the minimally modified Assess respiratory RIsk in Surgical patients in CATalonia (ARISCAT) and Laparoscopic Surgery Video Educational Guidelines (LAS VEGAS) scores with a higher AUC (LR: 0.824, ARISCAT: 0.672, LAS: 0.663). Independent risk factors based on multivariate LR mostly overlapped with Lasso-selected features, but lacked consistency with the important features using the Shapley additive explanation (SHAP) method of the LR model.</p><p><strong>Conclusions: </strong>The developed models, especially the DNN model and the nomogram, had good discrimination and calibration, and could be used for predicting PPCs in neurosurgical patients. The establishment of machine learning models and the ascertainment of risk factors might assist clinical decision support for improving surgical outcomes.</p><p><strong>Trial registration: </strong>ChiCTR 2100047474; https://www.chictr.org.cn/showproj.html?proj=128279 .</p>","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2170-2179"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2025-07-30DOI: 10.1097/CM9.0000000000003693
You Wu, Miao Ao, He Zhang, Kunyu Wang, Meixian Fang, Xueyan Lyu, Guobing Chen, Tao Lyu, Bin Li
{"title":"Value of adjuvant chemotherapy in IB-lIA cervical adenocarcinoma: A multicenter retrospective study.","authors":"You Wu, Miao Ao, He Zhang, Kunyu Wang, Meixian Fang, Xueyan Lyu, Guobing Chen, Tao Lyu, Bin Li","doi":"10.1097/CM9.0000000000003693","DOIUrl":"10.1097/CM9.0000000000003693","url":null,"abstract":"","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2192-2194"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2025-07-30DOI: 10.1097/CM9.0000000000003752
Ziyu Wang, Xuan Deng, Zhao Yang, Jiangtao Li, Pan Zhou, Wenlang Zhao, Yongchen Hao, Qiuju Deng, Na Yang, Lizhen Han, Yue Qi, Jing Liu
{"title":"Association between cardiovascular-kidney-metabolic health metrics and long-term cardiovascular risk: Findings from the Chinese Multi-provincial Cohort Study.","authors":"Ziyu Wang, Xuan Deng, Zhao Yang, Jiangtao Li, Pan Zhou, Wenlang Zhao, Yongchen Hao, Qiuju Deng, Na Yang, Lizhen Han, Yue Qi, Jing Liu","doi":"10.1097/CM9.0000000000003752","DOIUrl":"10.1097/CM9.0000000000003752","url":null,"abstract":"<p><strong>Background: </strong>The American Heart Association (AHA) introduced the concept of cardiovascular-kidney-metabolic (CKM) health and stage, reflecting the interaction among metabolism, chronic kidney disease (CKD), and the cardiovascular system. However, the association between CKM stage and the long-term risk of cardiovascular disease (CVD) has not been validated. This study aimed to evaluate the long-term CVD risk associated with CKM health metrics and CKM stage using data from a population-based cohort study.</p><p><strong>Methods: </strong>In total, 5293 CVD-free participants were followed up to around 13 years in the Chinese Multi-provincial Cohort Study (CMCS). Considering the pathophysiologic progression of CKM health metrics abnormalities (comprising obesity, central adiposity, prediabetes, diabetes, hypertriglyceridemia, CKD, and metabolic syndrome), participants were divided into CKM stages 0, 1, and 2. The time-dependent Cox regression models were used to estimate the cardiovascular risk associated with CKM health metrics and stage. Additionally, broader CVD outcomes were examined, with a specific assessment of the impact of stage 3 in 2581 participants from the CMCS-Beijing subcohort.</p><p><strong>Results: </strong>Among participants, 91.2% (4825/5293) had at least one abnormal CKM health metric, 8.8% (468/5293), 13.3% (704/5293), and 77.9% (4121/5293) were in CKM stages 0, 1, and 2, respectively; and 710 incident CVD cases occurred during a median follow-up time of 13.3 years (interquartile range: 12.1 to 13.6 years). Participants with each poor CKM health metric exhibited significantly higher CVD risk. Compared with stage 0, the hazard ratio (HR) (95% confidence interval [CI]) for CVD incidence was 1.31 (0.84-2.04) in stage 1 and 2.27 (1.57-3.28) in stage 2. Significant interactive impacts existed between CKM stage and age or sex, with higher CVD risk related to increased CKM stages in participants aged <60 years or females.</p><p><strong>Conclusion: </strong>These findings highlight the contribution of CKM health metrics and CKM stage to the long-term risk of CVD, suggesting the importance of multi-component recognition and management of poor CKM health in CVD prevention.</p>","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2139-2147"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2025-02-26DOI: 10.1097/CM9.0000000000003512
Meng Li, Xinxin Zhang, Lei Lin, Lei Miao, Haiyan Wu, Chunlei Zhou, Jing He
{"title":"Neuroblastoma risk decreased by NSUN3 rs7653521 C>T polymorphism in Chinese children.","authors":"Meng Li, Xinxin Zhang, Lei Lin, Lei Miao, Haiyan Wu, Chunlei Zhou, Jing He","doi":"10.1097/CM9.0000000000003512","DOIUrl":"10.1097/CM9.0000000000003512","url":null,"abstract":"","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2204-2206"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2025-07-22DOI: 10.1097/CM9.0000000000003541
Grace Oscullo, Jose Daniel Gómez-Olivas, Miguel Angel Martinez-Garcia
{"title":"Cancer and obstructive sleep apnea.","authors":"Grace Oscullo, Jose Daniel Gómez-Olivas, Miguel Angel Martinez-Garcia","doi":"10.1097/CM9.0000000000003541","DOIUrl":"10.1097/CM9.0000000000003541","url":null,"abstract":"","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2049-2053"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2025-08-04DOI: 10.1097/CM9.0000000000003748
Chiho Lee, Sydney Chi Wai Tang, Michael Kang Yin Lee, Ying Wai Ng, Johnny Wai Man Chan, Tak Mao Chan
{"title":"Cardiovascular-Kidney-Metabolic healthcare framework: Perspective from Hong Kong.","authors":"Chiho Lee, Sydney Chi Wai Tang, Michael Kang Yin Lee, Ying Wai Ng, Johnny Wai Man Chan, Tak Mao Chan","doi":"10.1097/CM9.0000000000003748","DOIUrl":"10.1097/CM9.0000000000003748","url":null,"abstract":"","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2054-2056"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Medical JournalPub Date : 2025-09-05Epub Date: 2025-08-04DOI: 10.1097/CM9.0000000000003626
Pan Yang, Jing Li, Zhangyong Song, Bin Chen, Shizhu Li
{"title":"Phage and enzyme therapies in wound infections: From lab to bedside.","authors":"Pan Yang, Jing Li, Zhangyong Song, Bin Chen, Shizhu Li","doi":"10.1097/CM9.0000000000003626","DOIUrl":"10.1097/CM9.0000000000003626","url":null,"abstract":"<p><strong>Abstract: </strong>Antibiotic-resistant (AR) bacterial wound infections (WIs) impose major burdens on healthcare systems, exacerbated by ineffective therapies and stalled antibiotic development. Phage therapy and phage-derived enzymes have gained traction as potent alternatives, leveraging targeted bactericidal mechanisms to combat AR pathogens. In this review, we summarised the antimicrobial mechanisms of both phage therapy and phage-derived enzymes as antimicrobial therapy, and outlined recent advances in their use for in vitro , in vivo and clinical applications for WI management. In addition, we also highlights recent advancements in their development, driven by genetic engineering, chemical modifications, and artificial intelligence. Finally, we identified the potential barriers and challenges they may encounter in clinical practice and the corresponding strategies to address these issues. The entire review gives us a comprehensive understanding of the latest advances in phages and their derivative enzyme therapies for treating WIs, in the hope that research in this field will continue to improve and innovate, accelerating the transition from the laboratory to application at the bedside and ultimately improving the efficacy of treatment for AR bacterial WIs.</p>","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":"2102-2115"},"PeriodicalIF":7.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Lin, Anyang Li, Linying Liu, Libin Zhang, Evangeline Li, Yang Sun
{"title":"Platinum-resistant ovarian cancer in China: Practice and challenges.","authors":"Jie Lin, Anyang Li, Linying Liu, Libin Zhang, Evangeline Li, Yang Sun","doi":"10.1097/CM9.0000000000003763","DOIUrl":"https://doi.org/10.1097/CM9.0000000000003763","url":null,"abstract":"","PeriodicalId":10183,"journal":{"name":"Chinese Medical Journal","volume":" ","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}