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Canonical and Non-Canonical Roles of SNX1 and SNX2 in Endosomal Membrane Dynamics. SNX1和SNX2在内体膜动力学中的典型和非典型作用。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-11-28 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231217867

Juliane Da Graça, Etienne Morel

{"title":"Canonical and Non-Canonical Roles of SNX1 and SNX2 in Endosomal Membrane Dynamics.","authors":"Juliane Da Graça, Etienne Morel","doi":"10.1177/25152564231217867","DOIUrl":"10.1177/25152564231217867","url":null,"abstract":"Sorting nexins (SNXs) are a family of membrane-binding proteins known to play a critical role in regulating endocytic pathway sorting and endosomal membrane trafficking. Among them, SNX1 and SNX2 are members of the SNX-BAR subfamily and possess a membrane-curvature domain and a phosphoinositide-binding domain, which enables their stabilization at the phosphatidylinositol-3-phosphate (PI3P)-positive surface of endosomes. While their binding to PI3P-positive platforms facilitates interaction with endosomal partners and stabilization at the endosomal membrane, their SNX-BAR region is pivotal for generating membrane tubulation from endosomal compartments. In this context, their primary identified biological roles-and their partnership-are tightly associated with the retromer and endosomal SNX-BAR sorting complex for promoting exit 1 complex trafficking, facilitating the transport of cargoes from early endosomes to the secretory pathway. However, recent literature indicates that these proteins also possess biological functions in other aspects of endosomal features and sorting processes. Notably, SNX2 has been found to regulate endosome-endoplasmic reticulum (ER) contact sites through its interaction with VAP proteins at the ER membrane. Furthermore, data from our laboratory show that SNX1 and SNX2 are involved in the tubulation of early endosomes toward ER sites associated with autophagy initiation during starvation. These findings shed light on a novel role of SNXs in inter-organelle tethering and communication. In this concise review, we will explore the non-retromer functions of SNX1 and SNX2, specifically focusing on their involvement in endosomal membrane dynamics during stress sensing and autophagy-associated processes.","PeriodicalId":101304,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231217867"},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138465366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic and Structural Analyses of VPS13 Proteins in Archaeplastida Reveal Their Complex Evolutionary History in Viridiplantae. 古塑菌VPS13蛋白的系统发育和结构分析揭示了其在植物中复杂的进化历史。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-11-27 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231211976

Sébastien Leterme, Olivier Bastien, Riccardo Aiese Cigliano, Alberto Amato, Morgane Michaud

{"title":"Phylogenetic and Structural Analyses of VPS13 Proteins in Archaeplastida Reveal Their Complex Evolutionary History in Viridiplantae.","authors":"Sébastien Leterme, Olivier Bastien, Riccardo Aiese Cigliano, Alberto Amato, Morgane Michaud","doi":"10.1177/25152564231211976","DOIUrl":"10.1177/25152564231211976","url":null,"abstract":"VPS13 is a lipid transfer protein family conserved among Eukaryotes and playing roles in fundamental processes involving vesicular transport and membrane expansion including autophagy and organelle biogenesis. VPS13 folds into a long hydrophobic tunnel, allowing lipid transport, decorated by distinct domains involved in protein localization and regulation. Whereas VPS13 organization and function have been extensively studied in yeast and mammals, information in organisms originating from primary endosymbiosis is scarce. In the higher plant Arabidopsis thaliana, four paralogs, AtVPS13S, X, M1, and M2, were identified, AtVPS13S playing a role in the regulation of root growth, cell patterning, and reproduction. In this work, we performed phylogenetic, as well as domain and structural modeling of VPS13 proteins in Archaeplastida in order to understand their general organization and evolutionary history. We confirmed the presence of human VPS13B orthologues in some phyla and described two new VPS13 families presenting a particular domain arrangement: VPS13R in Rhodophytes and VPS13Y in Chlorophytes and Streptophytes. By focusing on Viridiplantae, we were able to draw the evolutionary history of these proteins made by multiple gene gains and duplications as well as domain rearrangements. We showed that some Chlorophytes have only three (AtVPS13M, S, Y) whereas some Charophytes have up to six VPS13 paralogs (AtVPS13M1, M2, S, Y, X, B). We also highlighted specific structural features of VPS13M and X paralogs. This study reveals the complex evolution of VPS13 family and opens important perspectives for their functional characterization in photosynthetic organisms.","PeriodicalId":101304,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231211976"},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138465367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Nonvesicular Ceramide Transport. 非泡状神经酰胺转运机制。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2023-10-17 eCollection Date: 2023-01-01 DOI: 10.1177/25152564231208250

Lena Clausmeyer, Florian Fröhlich

{"title":"Mechanisms of Nonvesicular Ceramide Transport.","authors":"Lena Clausmeyer, Florian Fröhlich","doi":"10.1177/25152564231208250","DOIUrl":"https://doi.org/10.1177/25152564231208250","url":null,"abstract":"Ceramides, as key components of cellular membranes, play essential roles in various cellular processes, including apoptosis, cell proliferation, and cell signaling. Ceramides are the precursors of all complex sphingolipids in eukaryotic cells. They are synthesized in the endoplasmic reticulum and are further processed at the Golgi apparatus. Therefore, ceramides have to be transported between these two organelles. In mammalian cells, the ceramide transfer protein forms a contact site between the ER and the trans-Golgi region and transports ceramide utilizing its steroidogenic acute regulatory protein-related lipid transfer domain. In yeast, multiple mechanisms of nonvesicular ceramide transport have been described. This involves the nuclear-vacuolar junction protein Nvj2, the yeast tricalbin proteins, and the lipocalin-like protein Svf1. This review aims to provide a comprehensive overview of nonvesicular ceramide transport mechanisms and their relevance in cellular physiology. We will highlight the physiological and pathological consequences of perturbations in nonvesicular ceramide transport and discuss future challenges in identifying and analyzing ceramide transfer proteins.","PeriodicalId":101304,"journal":{"name":"Contact (Thousand Oaks (Ventura County, Calif.))","volume":"6 ","pages":"25152564231208250"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49687227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial-Endoplasmic Reticulum Interplay: A Lifelong On-Off Relationship? 线粒体-内质网相互作用：终生的开关关系？

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2019-01-01 Epub Date: 2019-07-19 DOI: 10.1177/2515256419861227

Corina T Madreiter-Sokolowski, Roland M Malli, Wolfgang F Graier

引用次数: 0

Catching Lipid Droplet Contacts by Proteomics. 通过蛋白质组学捕捉脂滴接触。

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2019-01-01 Epub Date: 2019-07-04 DOI: 10.1177/2515256419859186

Natalie Krahmer, Matthias Mann

引用次数: 0