Catching Lipid Droplet Contacts by Proteomics.

Natalie Krahmer, Matthias Mann
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引用次数: 0

Abstract

Lipid droplets (LDs), important organelles for energy storage and involved in the development of metabolic disorders, are extremely dynamic and interact with many other cellular compartments to orchestrate lipid metabolism. Little is known about how these organelle contacts are changed according to cellular needs and functions under different metabolic and pathological conditions and which proteins regulate this. Here, we summarize recent exciting discoveries about the reorganization of organelle contacts in steatotic liver, including the identification of novel LD contact site proteins in cell lines and in animals. We also discuss state of the art proteomics workflows that enable the characterization of LD-organelle contacts and tethering proteins and give an outlook how this can inform obesity research.

Abstract Image

Abstract Image

通过蛋白质组学捕捉脂滴接触。
脂滴(LDs)是一种重要的能量储存细胞器,参与代谢疾病的发展,它是非常动态的,并与许多其他细胞区室相互作用来协调脂质代谢。在不同的代谢和病理条件下,这些细胞器接触是如何根据细胞的需要和功能而改变的,以及哪些蛋白质调节这一点,人们知之甚少。在这里,我们总结了最近关于脂肪变性肝细胞器接触重组的令人兴奋的发现,包括细胞系和动物中新的LD接触位点蛋白的鉴定。我们还讨论了最先进的蛋白质组学工作流程,使ld -细胞器接触和拴系蛋白的表征成为可能,并展望了这将如何为肥胖研究提供信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
3.10
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