Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie最新文献_第2页

Degradation of 4-Chlorobenzoic Acid by a Pseudomonas sp. 假单胞菌降解4-氯苯甲酸的研究。

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80002-9

Uwe Klages, Franz Lingens

引用次数: 41

Contribution of DNA-DNA Hybridization to the Transfer of Enterobacter aerogenes to the Genus Klebsiella as K. mobilis DNA-DNA杂交对产气肠杆菌向克雷伯氏菌属转移的贡献

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80006-6

D. Izard, F. Gavini, P.A. Trinel, F. Krubwa, H. Leclerc

引用次数: 14

Identification of the Leaf Nodule Bacterial Strain PeH220 as Erwinia herbicola subsp. herbicola 叶根瘤菌株PeH220的鉴定为Erwinia除草剂亚种。herbicola

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80004-2

P. De Vos , K. Kersters, J. De Ley

引用次数: 2

Bakterienzahl und bakterielle Aktivität in Wasserkörpern unterschiedlicher Belastung 不同光线在水中的细菌数量和活动

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80009-1

Jenny Becker-Birck

引用次数: 0

2,3-Cleavage of Substituted Catechols in Nocardia sp. DSM 43251 (Rhodococcus rubrus) 2,3-红红球菌(Nocardia sp. DSM 43251)中取代儿茶酚的裂解

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80003-0

Hans Georg Rast, Gabriele Engelhardt, Peter R. Wallnöfer

{"title":"2,3-Cleavage of Substituted Catechols in Nocardia sp. DSM 43251 (Rhodococcus rubrus)","authors":"Hans Georg Rast, Gabriele Engelhardt, Peter R. Wallnöfer","doi":"10.1016/S0172-5564(80)80003-0","DOIUrl":"10.1016/S0172-5564(80)80003-0","url":null,"abstract":"<div>A catechol 2.3-dioxygenase, isolated from Nocardia sp. DSM 43251 (Rhodococcus rubrus) after induction with unpolar substituted phenols e.g. 4-(methylthio)-phenol or 4-methoxyphenol, was purified 79-fold by ammonium sulfate precipitation, acetone precipitation, and sephadex G 200 gel filtration. The molecular weight, as determined by gel filtration, was 125,000. SDS-polyacrylamide gel electrophoresis revealed three non-identical subunits with molecular weights from 40,000 to 50,000. Highest activity was obtained at pH 7.5 to 8.5. The enzyme was stable in the presence of oxygen, reducing and oxidizing agents, and at high temperatures with an optimum temperature of 60 °C. Atomic absorption spectrometry proved one atom of iron per molecule of enzyme which could not be removed by dialysis. The ring cleavage reaction was inhibited by metal ions like Ag+, Hg++ and Cu++ (10−6–10−5 M), and by 2,2′-bipyridyl (10−3 M).Catechol 2,3-dioxygenase from Nocardia sp. DSM 43251 showed a strict specificity for catechols with a substituent at C-4. Maximum reaction velocity was strongly influenced by the electronegativity of these substituents. Additional methyl groups at C-3 or C-5 decreased the affinity of the enzyme for the substrate as well as the reaction velocity. The structure of the reaction product and linear correlation of the reaction velocity with the tfp-values of the different catechols tested clearly indicate a 2,3-cleavage-mechanism.</div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 3","pages":"Pages 224-236"},"PeriodicalIF":0.0,"publicationDate":"1980-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80003-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121111225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Lactobacillus reuteri sp. nov., a New Species of Heterofermentative Lactobacilli 异发酵乳酸菌罗伊氏乳杆菌新种

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80007-8

Otto Kandler , Karl-Otto Stetter, Ruth Köhl

引用次数: 106

DNA-dependent RNA Polymerase of the Archaebacterium Methanobacterium thermoautotrophicum 热自养甲烷杆菌古细菌dna依赖性RNA聚合酶的研究

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80001-7

K.O. Stetter , J. Winter, R. Hartlieb

{"title":"DNA-dependent RNA Polymerase of the Archaebacterium Methanobacterium thermoautotrophicum","authors":"K.O. Stetter , J. Winter, R. Hartlieb","doi":"10.1016/S0172-5564(80)80001-7","DOIUrl":"10.1016/S0172-5564(80)80001-7","url":null,"abstract":"<div>The DNA-dependent RNA polymerase from Methanooacterium thermoautotrophicum was purified to homogeneity under the exclusion of oxygen. The subunit composition formula of the enzyme is: O: (96000)1; A: (74000)1; B: (59000)1; C: (50000)1; D: (33000)1; E: (24500)1; F: (10500)1; G: (6500)6. This results in a molecular weight of about 390000 for the complete enzyme. An incomplete particle lacking subunit O and demonstrating a five-fold lower specific activity can be isolated in addition to the complete enzyme by heparin cellulose chromatography. Maximal RNA synthesis is obtained in the presence of about 10 mM MgCl2 and 200 mM KCl at 50 or 60°C, depending on the template. As are the other archaebacterial RNA polymerases, this enzyme is insensitive to rifampicin and streptolydigin. The spacing of the heavy subunits and the existence of a fragment particle lacking the heaviest subunit are reminiscent of the halobacterial RNA polymerase.This enzyme is therefore clearly different from eubacterial RNA polymerases but it is similar to other archaebacterial RNA polymerases, thus supporting the theory of Carl Woese concerning the existence of two kingdoms of procaryotes.</div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 3","pages":"Pages 201-214"},"PeriodicalIF":0.0,"publicationDate":"1980-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80001-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130242696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Selective Medium for Isolating Staphylococci 葡萄球菌分离的选择性培养基

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-09-01 DOI: 10.1016/S0172-5564(80)80008-X

Karl-Heinz Schleifer , Eberhard Krämer

{"title":"Selective Medium for Isolating Staphylococci","authors":"Karl-Heinz Schleifer , Eberhard Krämer","doi":"10.1016/S0172-5564(80)80008-X","DOIUrl":"10.1016/S0172-5564(80)80008-X","url":null,"abstract":"<div>A selective medium was developed for isolating coagulase-positive and coagulase-negative staphylococci. The selective agents employed were sodium azide, potassium thiocyanate, lithium chloride and glycine. Addition of lithium chloride and glycine is only necessary if high counts of streptococci are expected. All type strains of the various staphylococcal species and other staphylococci tested revealed good growth on this new medium. S. intermedius was the only exception since its recovery was significantly lower than on control medium. However, normal growth of S. intermedius is possible at a lower sodium azide concentration (15–20 mg/litre). Growth of all micrococci and other bacteria such as various bacilli, arthrobacters, lactobacilli, streptococci, Escherichia coli, Enterobacter cloacae and Proteus mirabilis is inhibited: these organisms do not grow at all or at most as pinpoint-sized colonies. Addition of egg yolk or pork plasma to the medium may provide a basis for distinguishing S. aureus from coagulase-negative staphylococci. The new medium is not only useful for the selective isolation of staphylococci but also represents a new routine method für the separation of staphylococci from micrococci. The great specificity of this new medium could be demonstrated in detecting staphylococci in various foods at levels as low as 100/g.</div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 3","pages":"Pages 270-280"},"PeriodicalIF":0.0,"publicationDate":"1980-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80008-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127708414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Heterogeneity of the Species Lactobacillus acidophilus (Moro) Hansen and Moquot as Revealed by Biochemical Characteristics and DNA-DNA hybridisation 嗜酸乳杆菌Hansen (Moro)和Moquot (Moquot)生物化学特性和DNA-DNA杂交揭示的异质性

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-04-01 DOI: 10.1016/S0172-5564(80)80037-6

Eckhard Lauer, Christine Helming, Otto Kandler

{"title":"Heterogeneity of the Species Lactobacillus acidophilus (Moro) Hansen and Moquot as Revealed by Biochemical Characteristics and DNA-DNA hybridisation","authors":"Eckhard Lauer, Christine Helming, Otto Kandler","doi":"10.1016/S0172-5564(80)80037-6","DOIUrl":"https://doi.org/10.1016/S0172-5564(80)80037-6","url":null,"abstract":"<div>The “classical” and biochemical characters and the DNA-DNA homology of 37 strains of Lactobacillus acidophilus were determined. Two main homology groups, subdivided into 5 and 2 subgroups, respectively, were established. The subdivision into two main groups was found to correlate with the electrophoretic type of the L-lactic acid dehydrogenase and several features of the cell wall chemistry, whereas no phenotypical characters were found to distinguish the homology subgroups from each other. Therefore the subgroups were not named, although the homology among them was 50% or less, thus justifying their separation into distinct species. At present, only the two main homology groups are considered to constitute named species. The group which contains the type strain of L. acidophilus retains the epithet acidophilus, whereas the other group was recently described as the new species L. gasseri. The implications of the distinct heterogeneity of “L. acidophilus” for intestine bacteriology is discussed.</div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 2","pages":"Pages 150-168"},"PeriodicalIF":0.0,"publicationDate":"1980-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80037-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91773237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 123

Abbau von trans-Zimtsäure durch Chloridazon-abbauende Bakterien 通过克罗能分解细菌分解渗透酸

Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1980-04-01 DOI: 10.1016/S0172-5564(80)80034-0

Uwe Tittmann, Willi Wegst, Renate Blecher, Franz Lingens

引用次数: 5