Abbau von trans-Zimtsäure durch Chloridazon-abbauende Bakterien

Abstract

A chloridazone-degrading bacterium strain E incubated with trans-cinnamic acid accumulated cis-2.3-dihydro-2.3-dihydroxy-trans-cinnamic acid in the culture medium. A chloridazone-degrading bacterium strain N incubated with trans-cinnamic acid, accumulated 2.3-dihydroxy-trans-cinnamic acid in the culture medium. Each metabolite was isolated in crystalline form and identified by a variety of conventional chemical techniques-Catechol-2.3-dioxygenase prepared from the strain E converted 2.3-dihydroxy-trans-cinnamic acid to the meta cleavage product. Cell extracts converted the yellow ring fission product. The production of fumaric acid could be detected with high performance liquid chromatography and with fumarase.