Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie最新文献

{"title":"Immunität gegen Erysipelothrix rhusiopathiae-Infektion durch aktive Immunisierung mit homologer Neuraminidase","authors":"Hans E. Müller ,&nbsp;Christina Krasemann","doi":"10.1016/S0300-872X(76)80038-8","DOIUrl":"10.1016/S0300-872X(76)80038-8","url":null,"abstract":"<div><p>Neuraminidase may play a role as a pathogenic factor in Erysipelothrix rhusiopathiae infection. The protective effect of active immunization with purified neuraminidase was therefore tested in an infection experiment in white mice.</p><p>Mice were immunized 2, 4, 6, 8 or 10 times i.p. with Erysipelothrix neuraminidase. A control group received 10 injections with physiological saline. The infective dose varied between 7 and 7 × 10⁷ cells. While all control animals infected with 7 × 10¹ germs died, the lethal effect could be reduced to 50% and 25% in animals immunized twice and 4 times, respectively. Only animals immunized 8 and 10 times were still partly protected against germ numbers of 10³-10⁴. Germ numbers of 10⁵ and more were almost always fatal even in highly immunized animals. Even a high immunization with neuraminidase could only lower the lethal by a maximum factor of 10⁴ germs used.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 3","pages":"Pages 237-241"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80038-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73017129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"J. Potel","doi":"10.1016/S0300-872X(76)80018-2","DOIUrl":"10.1016/S0300-872X(76)80018-2","url":null,"abstract":"","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Page 187"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80018-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81299790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple Test for Immunogenicity of Colloidal Infusion Solutions - the Draining Lymph Node Activation","authors":"Lidka Korčáková ,&nbsp;E. Paluska ,&nbsp;Věra Hašková ,&nbsp;J. Kopeček","doi":"10.1016/S0300-872X(76)80036-4","DOIUrl":"https://doi.org/10.1016/S0300-872X(76)80036-4","url":null,"abstract":"<div><p>The increasing number of lymphocytes with nucleoli synthesizing RNA in the mouse lymph nodes, draining the site of injection of infusion solutions, was used as a marker for their immunogenicity. The percentage of «active» lymphocytes significantly increased 3 days after the administration of preparations based on bovine serum or human haemoglobin which were found immunogenic when testing for antibody formation. Such a reaction was not elicited in mice treated with Physiogel, Dextran and Duxon which do not cause any production of antibodies. The described test may serve as a rapid and economical assay for the immunogenicity of infusion solutions and other substances.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 3","pages":"Pages 219-223"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80036-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91976781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"E. Harbers","doi":"10.1016/S0300-872X(76)80017-0","DOIUrl":"10.1016/S0300-872X(76)80017-0","url":null,"abstract":"","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 186-187"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80017-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79813500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"E. Kölsch","doi":"10.1016/S0300-872X(76)80015-7","DOIUrl":"https://doi.org/10.1016/S0300-872X(76)80015-7","url":null,"abstract":"","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 184-185"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80015-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136675641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"G. Riethmüller","doi":"10.1016/S0300-872X(76)80021-2","DOIUrl":"10.1016/S0300-872X(76)80021-2","url":null,"abstract":"","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 188-189"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80021-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"95124166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demonstration of cellular antibodies against streptococcal antigens in children","authors":"Heide Knöll ,&nbsp;G. Knöll ,&nbsp;W. Köhler","doi":"10.1016/S0300-872X(76)80005-4","DOIUrl":"10.1016/S0300-872X(76)80005-4","url":null,"abstract":"<div><p>In a group of 48 children the following streptococcal antigens were tested by means of the lymphocyte transformation test: group A streptococcal M1- and M19-protein, mucopeptide, polysaccharides from group A and group C streptococci, erythrogenic toxins and streptokinase. Specific transformations were obtained only by M-proteins. Erythrogenic toxines induced an unspecific stimulation comparable to the mitogenic activity of phytohaemagglutinins. No reactions could be found with streptococcal mucopeptide, the group-specific carbohydrates of groups A and C-streptococci and streptokinase, resp.</p><p>A significant difference was seen in the group of young children (mean age 5 years) between the specific transformability by M1-protein (a common type) and M19-protein (a rare type). No difference was seen with both M-proteins in the group of older children (mean age 11 years). In both groups of children no differences exist in the number of positive cutaneous reactions after injection of M1- and M19-proteins, but the intensity of reactions (diameters of erythema) were more pronounced with the M-protein of the common type 1.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 143-152"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80005-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124496987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"H. Brandis","doi":"10.1016/S0300-872X(76)80011-X","DOIUrl":"https://doi.org/10.1016/S0300-872X(76)80011-X","url":null,"abstract":"","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Page 182"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80011-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136850348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strong Expression of Fc-Receptors on Leukemic Cells in Hairy Cell Leukemia","authors":"E.P. Rieber ,&nbsp;J.G. Saal ,&nbsp;G. Riethmüller ,&nbsp;H.W.v. Heyden ,&nbsp;H.D. Waller","doi":"10.1016/S0300-872X(76)80043-1","DOIUrl":"10.1016/S0300-872X(76)80043-1","url":null,"abstract":"<div><p>The neoplastic cells of a patient with hairy cell leukemia were found to express a high Fe-receptor activity on their surface. When the Fc-receptors were determined quantitatively by measuring the uptake of ¹²⁵I-labelled aggregated IgG under saturation conditions, it was found that the hairy cells bound approximately 3 times more aggregated IgG than normal adherent mononuclear cells. The bound ¹²⁵I-Agg. IgG was evenly distributed on the cell surface when the cells were labelled at 4°C and was rapidly redistributed in form of a cap at one cell pole during incubation at 20°C.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 3","pages":"Pages 282-288"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80043-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125373441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous and Antibody-Dependent Cellular Cytotoxicity in Melanoma Patients and Healthy Control Persons","authors":"Hans-Hartmut Peter ,&nbsp;Friedhelm Knoop,&nbsp;Joachim Robert Kalden","doi":"10.1016/S0300-872X(76)80042-X","DOIUrl":"10.1016/S0300-872X(76)80042-X","url":null,"abstract":"<div><p>Spontaneous cell-mediated cytotoxicity (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) against ⁵¹Cr labeled allogeneic target cells of a human melanoma cell line (IGR 3) were determined with purified effector lymphocytes and defibrinated whole blood from 14 melanoma patients and 13 healthy control persons. Peripheral blood lymphocytes were isolated by Ficoll gradient centrifugation (fraction F) ; subsequently the phagocytic and adherent cells were removed and the supernating cell population (fraction FFF) was passaged through IgG anti-IgG columns to obtain a B cell free lymphocyte suspension (fraction FFF-C). Cells from the 3 lymphocyte fractions and from defibrinated whole blood were simultaneously tested for cytotoxic activity against unsensitized IGR 3 target cells (SCMC assay) and IGR 3 cells previously sensitized with a rabbit anti-melanoma IgG (ADCC assay). Dose-response curves were established with all lymphocyte fractions and with whole blood.</p><p>The following results were obtained:</p><ul><li><span>1.</span><span><p>With all lymphocyte fractions tested, ADCC was approximately 15 times higher than SCMC, whereas with whole blood, the difference tended to be less pronounced.</p></span></li><li><span>2.</span><span><p>Elimination of phagocytic and adherent cells had no significant effect on SCMC and ADCC.</p></span></li><li><span>3.</span><span><p>Passage over IgG anti-IgG columns drastically reduced cytotoxicity in both assays without, however, completely abolishing it.</p></span></li><li><span>4.</span><span><p>The only difference seen between lymphocyte cytotoxicity of melanoma patients and control persons was a slight, but non-significant depression of SCMC and ADCC in melanoma patients</p></span></li></ul>.<p>The results confirm and extend our previous report that SCMC against an allogeneic tumor cell-line is due to non-specific «Null» or «K» cell-activity rather than to specific T cell cytotoxicity. In one experiment freshly explanted melanoma cells were labeled with ⁵¹Cr and reacted with autologous blood and purified lymphocyte fractions. It was found that cellular cytotoxicity depending on serum factors (ADCC) was an effective lytic mechanism, whereas T cell-mediated cytotoxicity could not be demonstrated.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 3","pages":"Pages 263-281"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80042-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122874301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}