{"title":"Immunität gegen Erysipelothrix rhusiopathiae-Infektion durch aktive Immunisierung mit homologer Neuraminidase","authors":"Hans E. Müller , Christina Krasemann","doi":"10.1016/S0300-872X(76)80038-8","DOIUrl":"10.1016/S0300-872X(76)80038-8","url":null,"abstract":"<div><p>Neuraminidase may play a role as a pathogenic factor in Erysipelothrix rhusiopathiae infection. The protective effect of active immunization with purified neuraminidase was therefore tested in an infection experiment in white mice.</p><p>Mice were immunized 2, 4, 6, 8 or 10 times i.p. with Erysipelothrix neuraminidase. A control group received 10 injections with physiological saline. The infective dose varied between 7 and 7 × 10<sup>7</sup> cells. While all control animals infected with 7 × 10<sup>1</sup> germs died, the lethal effect could be reduced to 50% and 25% in animals immunized twice and 4 times, respectively. Only animals immunized 8 and 10 times were still partly protected against germ numbers of 10<sup>3</sup>-10<sup>4</sup>. Germ numbers of 10<sup>5</sup> and more were almost always fatal even in highly immunized animals. Even a high immunization with neuraminidase could only lower the lethal by a maximum factor of 10<sup>4</sup> germs used.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 3","pages":"Pages 237-241"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80038-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73017129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Wiedermann , H. Stemberger, O. Förster, M. Müller
{"title":"BPO-Specific, Complement-Dependant Cell-Lysis of Differently Sensitized Sheep Red Cells: Evaluation of Haptenic Groups and their Influence on IgM- and IgG-induced Lysis","authors":"G. Wiedermann , H. Stemberger, O. Förster, M. Müller","doi":"10.1016/S0300-872X(76)80008-X","DOIUrl":"10.1016/S0300-872X(76)80008-X","url":null,"abstract":"<div><p>Sheep erythrocytes were coated with bencylpenicilloyl-(BPO)groups. Different incubation periods resulted in erythrocyte preparations with different hapten density. Complement dependent lysis induced by IgM or IgG antibodies was studied with the cell preparations. The calculation of hapten density on the erythrocyte surface was not possible by direct measurement of coupled radioactive BPO, since more than 90% of radioactive material was found in the soluble supernatant after osmotic cell lysis and less than 10% was fixed to the cellular membrane. Measurement of membrane bound immunologically relevant BPO-groups was achieved, therefore, by comparison of the inhibitory capacity of the test cells with that of a standard cell preparation. The latter consisted of tannic acid treated erythrocytes coated with protein complexed radioactive BPO. Surface hapten density of the different target cell preparations varied between 1.9 × 10<sup>5</sup> and 4.8 × 10<sup>5</sup> BPO-groups per cell depending on the time of incubation. Complement dependent antibody mediated cell lysis was significantly reduced by reduction of haptenic sites per target cell, IgG induced lysis being much more affected than hemolysis induced by IgM antibodies. Statistical calculations led to the conclusion that 18,000 protein islets per cell bearing 4 or more BPO-groups are not sufficient for hemolysis induced by IgG antibodies. 48,000 protein islets with this hapten density are necessary for «optimal« sensitization. IgG antibodies must be apparently bound to the cell surface in bivalent form.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 173-180"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80008-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125563809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Demonstration of cellular antibodies against streptococcal antigens in children","authors":"Heide Knöll , G. Knöll , W. Köhler","doi":"10.1016/S0300-872X(76)80005-4","DOIUrl":"10.1016/S0300-872X(76)80005-4","url":null,"abstract":"<div><p>In a group of 48 children the following streptococcal antigens were tested by means of the lymphocyte transformation test: group A streptococcal M1- and M19-protein, mucopeptide, polysaccharides from group A and group C streptococci, erythrogenic toxins and streptokinase. Specific transformations were obtained only by M-proteins. Erythrogenic toxines induced an unspecific stimulation comparable to the mitogenic activity of phytohaemagglutinins. No reactions could be found with streptococcal mucopeptide, the group-specific carbohydrates of groups A and C-streptococci and streptokinase, resp.</p><p>A significant difference was seen in the group of young children (mean age 5 years) between the specific transformability by M1-protein (a common type) and M19-protein (a rare type). No difference was seen with both M-proteins in the group of older children (mean age 11 years). In both groups of children no differences exist in the number of positive cutaneous reactions after injection of M1- and M19-proteins, but the intensity of reactions (diameters of erythema) were more pronounced with the M-protein of the common type 1.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 143-152"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80005-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124496987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Strong Expression of Fc-Receptors on Leukemic Cells in Hairy Cell Leukemia","authors":"E.P. Rieber , J.G. Saal , G. Riethmüller , H.W.v. Heyden , H.D. Waller","doi":"10.1016/S0300-872X(76)80043-1","DOIUrl":"10.1016/S0300-872X(76)80043-1","url":null,"abstract":"<div><p>The neoplastic cells of a patient with hairy cell leukemia were found to express a high Fe-receptor activity on their surface. When the Fc-receptors were determined quantitatively by measuring the uptake of <sup>125</sup>I-labelled aggregated IgG under saturation conditions, it was found that the hairy cells bound approximately 3 times more aggregated IgG than normal adherent mononuclear cells. The bound <sup>125</sup>I-Agg. IgG was evenly distributed on the cell surface when the cells were labelled at 4°C and was rapidly redistributed in form of a cap at one cell pole during incubation at 20°C.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 3","pages":"Pages 282-288"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80043-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125373441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunologic Effects of Morphine in Rodents, Rabbits, Monkeys, and Cats1)","authors":"David A. Ringle , Betty L. Herndon","doi":"10.1016/S0300-872X(76)80003-0","DOIUrl":"10.1016/S0300-872X(76)80003-0","url":null,"abstract":"<div><p>The effects of prolonged morphine administration on immunologic reactivity against morphine was studied in a number of animal species: rabbit, monkey, guinea pig, rat, and cat. Some evidence for increased serum binding of <sup>14</sup>C-labeled morphine was noted after morphine treatment in all test species, with the rabbit the best responder and the cat showing little or no response. In addition to measurements on serum binding of <sup>14</sup>C-labeled morphine, other methods (measurement of serum binding of <sup>14</sup>C-labeled codeine and methadone, competitive inhibition tests, radial immunodiffusion, and passive hemagglutination) were used for one or more of the species. Overall, results with these test methods have shown that prolonged morphine administration can result in immunologic responsiveness to morphine in animals.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 2","pages":"Pages 126-137"},"PeriodicalIF":0.0,"publicationDate":"1976-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80003-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122001260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}