{"title":"Studies on the Mechanism of Specific Immunological Unresponsiveness","authors":"Vesna Tomažič , B. Vitale","doi":"10.1016/S0300-872X(76)80047-9","DOIUrl":"10.1016/S0300-872X(76)80047-9","url":null,"abstract":"<div><p>The immunological capacity of lymphoid cells from mice rendered tolerant to high and low doses of BSA was investigated. The tolerance was induced by multiple injections of high and low doses of antigen through the period of 30 days. Lymph node and bone marrow cells from tolerant animals were transferred into lethally irradiated syngeneic recipients. After 10 days, when lymphoid organs of the recipients were repopulated with the injected cells, challenge injection of the same antigen incorporated into complete Freund's adjuvant was given. The immune response of the transferred cells in the recipients was evaluated by analysis of the specific antibodies in the sera. Lymphoid cells from donors rendered tolerant with high doses of antigen recovered their reactivity 20 days after the transfer to the level of reaction of normal cells. Lymphoid cells from donors receiving multiple injection of low doses of BSA remained tolerant after the transfer through the entire observation period. According to the cellular events in the donors during the period of tolerance induction, and the behaviour of the transferred lymphoid cells in the new recipients, it seems possible that tolerance induced with high doses of BSA corresponded to the B-cell tolerance, while low doses of antigen most likely induced tolerance of T-cell population. The possible cellular mechanisms of B and T-cell tolerance were discussed.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 1","pages":"Pages 13-21"},"PeriodicalIF":0.0,"publicationDate":"1976-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80047-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133765314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Histoautoradiographic Study of the Localization of Antigen and Specific Antibodies in the Rabbit Spleen","authors":"L. Rodák","doi":"10.1016/S0300-872X(76)80049-2","DOIUrl":"10.1016/S0300-872X(76)80049-2","url":null,"abstract":"<div><p>The localization of antigen (chicken serum albumin) and specific antibodies in sections from the spleens of rabbits after primary and secondary immunization was studied by means of histoautoradiography.</p><p>After primary immunization, both antigen and antibodies were demonstrated in the lymphoid follicles consistently between 3 and 42 days after injection. Antigen was detected in addition in the marginal zone on the 5th day, and antibodyforming cells in the marginal zone and periarteriolar lymphocyte sheaths between 3 and 9 days after immunization.</p><p>After secondary immunization, follicular antigen trapping was detected as early as 2 hours after injection, reaching a peak in the follicles and marginal zone on the 3rd day after immunization. At later periods of time the presence of antigen in the follicles was less prominent than that of specific antibodies. The numbers of antibody-forming cells were 10–100-fold higher in the secondary than in the primary response.</p><p>The findings are discussed in relation to the role of antigen-antibody complex in the lymphoid follicles in the development of the capacity to produce the secondary type of immune response.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"151 1","pages":"Pages 32-45"},"PeriodicalIF":0.0,"publicationDate":"1976-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80049-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115945356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}