Water Biology and Security最新文献

{"title":"Moving bed biofilm reactor for Pimelodus maculatus reared in RAS: Start-up maturation, bioreactor microbiome and nitrogen removal","authors":"Augusto Costa Cardoso ,&nbsp;Vinicius Muller Buratto ,&nbsp;Higor Tenfen ,&nbsp;Marco Shizuo Owatari ,&nbsp;Katt Regina Lapa","doi":"10.1016/j.watbs.2024.100251","DOIUrl":"10.1016/j.watbs.2024.100251","url":null,"abstract":"<div><p>Moving bed biofilm reactors (MBBR) are equipment used to remove organic compounds and are frequently tested for aquaculture operations aimed at treating and reusing water. However, a limitation of MBBR is the long maturation period necessary for nitrification to occur efficiently. We evaluated biofilm maturation in MBBR with biomass adhered to support material using enriched medium-NH₄Cl and a treatment without enrichment. Two independent recirculating aquaculture systems were established based on a freshwater fish farm that produced a daily total ammonia nitrogen concentration of 1.0 ​mg/L. Each treatment was composed of a reservoir and two experimental bioreactors that received support materials for the colonization of the adhered biomass. The results indicated that the enriched bioreactors performed slower, taking 29 days to reach maturity, while the control treatment matured at 27 days. The species <em>Candidatus Nitrospira defluvii,</em> responsible for acting in the nitrification process became more abundant in the control treatment where it represented 23% of the biofilm composition, while the enriched treatment presented only 3%. The results suggest that the nitrification process in the control treatment performed better than in the enriched bioreactors. However, the use of synthetic media such as NH₄Cl and sodium acetate as sources of C:N associated with the <em>Pimelodus maculatus</em> effluent during the start-up process had an important benefit to the maturation time of both bioreactors.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100251"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000076/pdfft?md5=49cb330c3b6da6b13f1be081cee8e989&pid=1-s2.0-S2772735124000076-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140406548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of microbial communities and antibiotic resistance genes in typical rivers of the western Qinghai Lake basin","authors":"Jia Jia ,&nbsp;Xiaoyan Xi ,&nbsp;Xin Li ,&nbsp;Hongjuan Hu ,&nbsp;Kelong Chen ,&nbsp;Chenxi Wu","doi":"10.1016/j.watbs.2024.100249","DOIUrl":"10.1016/j.watbs.2024.100249","url":null,"abstract":"<div><p>Environmental factors and anthropogenic activity are key factors that shape the distribution of bacteria and antibiotic resistance genes (ARGs) in natural environments. However, few studies have focused on the occurrence of bacteria and ARGs in remote and pristine environments. In this study, the distribution of bacteria and ARGs in two typical rivers of the western Qinghai Lake basin was investigated. Results showed that cold-resistant <em>Planomicrobium</em> sp. was the predominant genus due to the low temperature, followed by <em>unclassified_f_Planococcaceae</em>. High nitrogen nutrients increased the abundance and diversity of the bacteria community and denitrification was the dominant means of bacterial nitrate reduction. Tetracycline resistance genes including <em>tet</em>A, <em>tet</em>B, and <em>tet</em>C were the dominant ARGs in the western Qinghai Lake basin, ranging from 2.30 ​× ​10³ to 1.91 ​× ​10⁸ copies/L, while the low abundances of <em>int</em>I1, and ARGs such as <em>sul</em>1 indicated low anthropogenic activity in the western Qinghai Lake basin. Finally, the strong positive correlation between ARGs and <em>int</em>I1 highlights the potential transmission risk of ARGs through cross- or co-selection by horizontal gene transfer. Our study emphasized the adaptation of bacteria to the environment and the facilitation of anthropogenic activity in the propagation of ARGs in natural environments.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100249"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000052/pdfft?md5=35ad1889c7a1d257f1a89cb01c4c4afd&pid=1-s2.0-S2772735124000052-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140088992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A one-health approach to non-native species, aquaculture, and food security","authors":"Rodolphe E. Gozlan ,&nbsp;Claudia Bommarito ,&nbsp;Marta Caballero-Huertas ,&nbsp;Justina Givens ,&nbsp;Jean-Michel Mortillaro ,&nbsp;Elodie Pepey ,&nbsp;Ralien Purco Ralaiarison ,&nbsp;Paula Senff ,&nbsp;Marine Combe","doi":"10.1016/j.watbs.2024.100250","DOIUrl":"10.1016/j.watbs.2024.100250","url":null,"abstract":"<div><p>One-Health is an umbrella term that integrates the health of the environment, humans and non-human animals. This approach is applied here to elucidate the impact of non-native invasive species on aquaculture and food security. Despite inherent biases against these species, a better understanding of their characteristics allows for the identification of those of greatest concern, minimizing the risk of food shortages and infectious diseases. This review summarises the positive and negative impacts of non-native species, delineating the specific areas they may impact. Additionally, this review gives an insight to the expertise and stakeholders that would need to be included if a “One-Health” approach were to be implemented by policymakers to better control non-native species.</p><p>Detailed examples illustrate the consequences of non-native species on trophic dynamics, ecosystem health, water chemistry, and human health, emphasizing the importance of managing them within a multidimensional framework. The “One-Health” approach is explained, and suggestions are made on how certain non-native species could be used to contribute to food security in low- and middle-income countries. Furthermore, recommendations are made to promote a more inclusive management strategy.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100250"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000064/pdfft?md5=83ac24b011449d9e4b758aa2fb59e0dc&pid=1-s2.0-S2772735124000064-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140271559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LxIS motif is the language between virus and host RLRs: From fish to mammalian viruses","authors":"Shun Li ,&nbsp;Long-Feng Lu ,&nbsp;Can Zhang","doi":"10.1016/j.watbs.2024.100246","DOIUrl":"10.1016/j.watbs.2024.100246","url":null,"abstract":"<div><p>Regulation of host interferon (IFN) production is a crucial strategy for viral immune escape. We propose that the LxIS motif language, a marker for exogenous viral proteins, interacts with host retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) factors. In this study, the viral proteins containing the LxIS motif of grass carp reovirus (GCRV), and cyprinid herpesvirus 3 (CyHV-3), which belong to the fish RNA and DNA viruses, were found to interact with RLRs, and the general functions of these proteins were negative regulation of IFN activation. Terrestrial animal viruses with this motif language, such as SARS-CoV-2 and so on, are also examined. Several viral proteins that were not reported previously were found to be associated with the RLR pathway. Taken together, the LxIS motif language elucidates a straightforward and efficient method for identifying viral proteins that target host RLRs to regulate IFN expression, and is conserved in both lower and higher vertebrates.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100246"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000027/pdfft?md5=d6551696a0359e530a152bc96e21506b&pid=1-s2.0-S2772735124000027-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel cell line established from skin tissue of gibel carp (Carassius gibelio) is susceptible to Carassius auratus herpesvirus infection","authors":"Qi-Qi Jiang ,&nbsp;Zhi Li ,&nbsp;Qi-Ya Zhang ,&nbsp;Fei Ke","doi":"10.1016/j.watbs.2024.100253","DOIUrl":"10.1016/j.watbs.2024.100253","url":null,"abstract":"<div><p>Frequent outbreaks of emerging infectious diseases in fish, such as <em>Carassius auratus</em> herpesvirus (CaHV) infection has caused great economic losses in China. However, the lack of a sensitive cell culture system has limited studies of CaHV. In the present study, a new cell line (gibel carp skin cell, GiCS) derived from gibel carp (<em>Carassius gibelio</em>) skin tissue was established to create a valuable tool for research of the virus. The GiCS cells consisted mainly of epithelial-like cells, which grew well at 25 ​°C in L-15 medium supplemented with 10–20% fetal bovine serum. Chromosomal analysis revealed that the skin cell line remained amphitriploid, with most chromosome counts being 156 (54%). The GiCS cells can be efficiently transfected and expressed exogenous genes. In particular, the GiCS cells showed high susceptibility to CaHV infection, which was confirmed by virus infection tests, detection of viral gene expression, and ultrastructural observation. To our knowledge, it is the first cell line that is highly permissive to CaHV infection. In addition, the cells also showed susceptibility to several aquatic animal viruses from different families including <em>Iridoviridae</em>, <em>Rhabdoviridae</em>, and <em>Reoviridae</em>. In conclusion, these results indicated that the establishment of the GiCS cell line is a significant advance that will be beneficial to future studies of CaHV and other aquatic animal viruses.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100253"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277273512400009X/pdfft?md5=6973549fed436f9a79fdc1cb190f8efc&pid=1-s2.0-S277273512400009X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of stmn1a and genetic effects of a 22-bp Indel in its promoter region of bighead carp Hypophthalmichthys nobilis","authors":"Xiaoyu Zhou ,&nbsp;Yanhong Wu ,&nbsp;Junru Wang ,&nbsp;Xiaomu Yu ,&nbsp;Jingou Tong","doi":"10.1016/j.watbs.2024.100254","DOIUrl":"10.1016/j.watbs.2024.100254","url":null,"abstract":"<div><p>Stathmin 1 (<em>stmn1</em>) gene, which is involved in the control of cell cycle progression, cell proliferation, differentiation and motility, is a crucial relay in the signal transduction of multiple signaling pathways. Reports on the function of <em>stmn1</em> in fish are scarce. In this study, the characterization and function of <em>stmn1a</em> gene were explored in an important food fish, namely bighead carp (<em>Hypophthalmichthys nobilis</em>). The bighead carp <em>stmn1a</em> (<em>Hynstmn1a</em>) genomic sequence, which has 2995 bp with an ORF of 447 bp, is evolutionarily conserved in teleost fish. The highest level of expression of <em>Hynstmn1a</em> is in the un-fertilized eggs and spleen of adult bighead carp. The overexpression of <em>Hynstmn1a</em> facilitates cell cycle progression and suppresses apoptosis in EPC cells. In an siRNA assay, disruption of <em>stmn1a</em> in EPC cells resulted in the inhibition of cell cycle progression and promotion of apoptosis. Analysis of promoter activity indicated that an upstream transcription factor (foxo4) regulates <em>Hynstmn1a</em>. Zebrafish mutants with <em>stmn1a</em> knockout by CRISPR/Cas9 exhibited a significant retardation of body length (<em>p</em> ​&lt; ​0.05). There was no significant difference in body weight between <em>stmn1a</em>-mutant zebrafish and wild-type (<em>p</em> ​= ​0.053). A 22-bp Indel in the promoter region of <em>Hynstmn1a</em> with an observed heterozygosity (Ho) of 0.136 in a bighead carp population (<em>n</em> ​= ​191). The polymorphism of this 22-bp Indel genotype is significantly associated with body length (BL), head length (HL) (<em>p</em> ​&lt; ​0.05), body weight (BW), head height (HH), and head width (HW) (<em>p</em> ​&lt; ​0.01), showing its genetic effects on growth promotion. This study sheds light on the function of <em>stmn1a</em> in fish and demonstrates the potential of the 22-bp Indel of <em>Hynstmn1a</em> as a gene marker when selecting breeding individuals for faster growth and a bigger head of bighead carp in aquaculture.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100254"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000106/pdfft?md5=3254326309d5ea5e38dc120f43ee62ad&pid=1-s2.0-S2772735124000106-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish usp3 loss promotes hypoxic tolerance by disrupting deubiquitination of K63-polyubiquitinated hif-1αa","authors":"Jun Li ,&nbsp;Ziwen Zhou ,&nbsp;Xiaolian Cai ,&nbsp;Yanan Song ,&nbsp;Zhi Li ,&nbsp;Ziyi Li ,&nbsp;Wuhan Xiao ,&nbsp;Jing Wang","doi":"10.1016/j.watbs.2024.100245","DOIUrl":"10.1016/j.watbs.2024.100245","url":null,"abstract":"<div><p>The hypoxia-inducible factor (HIF)-mediated hypoxia signaling pathway influences oxygen homeostasis in aerobic organisms. This pathway is regulated by several other pathways. Elucidation of its regulation and the underlying mechanisms may provide insights into the strategies of adaptation and tolerance of organisms to hypoxia. In this study, we found that loss of ubiquitin-specific protease 3 (usp3) in zebrafish promotes hypoxia tolerance. Zebrafish usp3 specifically binds to hif-1αa and induces its proteasomal degradation, which is dependent on its deubiquitinase activity. This process leads to the suppression of hypoxia signaling under hypoxia. In addition, usp3 catalyzes the deubiquitination of K63-polyubiquitinated hif-1αa. Endogenous evidence indicated that mammalian USP3 behaves like zebrafish usp3 in regulating the activity of HIF-1α. These findings revealed a novel role for usp3 in influencing hypoxia signaling and showed that usp3-mediated HIF-1α degradation impairs hypoxia signaling, leading to a decrease in hypoxia tolerance.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100245"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000015/pdfft?md5=348303f64a87d878fced2e2fbb49bee5&pid=1-s2.0-S2772735124000015-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139393731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best paper awards 2023","authors":"","doi":"10.1016/j.watbs.2024.100263","DOIUrl":"https://doi.org/10.1016/j.watbs.2024.100263","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100263"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000192/pdfft?md5=606fa63046089cb75be12f2edacac4e4&pid=1-s2.0-S2772735124000192-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgement of reviewers who reviewed for Water Biology and Security in 2023","authors":"","doi":"10.1016/j.watbs.2024.100264","DOIUrl":"https://doi.org/10.1016/j.watbs.2024.100264","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100264"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000209/pdfft?md5=1969321f678fb3971c089e562aa976d6&pid=1-s2.0-S2772735124000209-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-species probiotics enhance survival, growth, intestinal microbiota and disease resistance of rohu (Labeo rohita) larvae","authors":"Zannatul Ferdous ,&nbsp;Md Kabir Hossain ,&nbsp;Md Hadiuzzaman ,&nbsp;S.M. Rafiquzzaman ,&nbsp;KM Abdul Halim ,&nbsp;Tanvir Rahman ,&nbsp;Md Ali Reza Faruk ,&nbsp;Zulhisyam Abdul Kari ,&nbsp;Md Shahjahan","doi":"10.1016/j.watbs.2023.100234","DOIUrl":"10.1016/j.watbs.2023.100234","url":null,"abstract":"<div><p>Multi-strain probiotics provide the most dependable approaches to improve health, immune response, and disease resistance in farmed fishes. In the present study, we examined the effects of multi-species probiotics on the survival, growth, immune response, and disease resistance of rohu (<em>Labeo rohita</em>) larvae. Newly hatched larvae from the day of first feeding (average weight of 0.003 ​g) were reared with multi-species probiotics having a combination of <em>Bacillus subtilis</em> (10⁹ colony forming units (cfu)/mL) and <em>Lactobacillus</em> spp. (<em>Lactobacillus plantarum, Lactobacillus buchneri -</em> 10¹¹ ​cfu/mL) in water containing doses of 0 (control-C), 0.5 ​mL/L (treatment 1-T1), and 1.0 ​mL/L (treatment 2-T2) in triplicates for 90 days. After the experiment, a challenge test was performed to assess the fish's resistance to pathogenic <em>Aeromonas veronii</em>. Significantly higher survival was recorded in larvae of treated groups (87% in T2 and 79% in T1) compared to the control (62%). Significantly higher growth performance (weight gain and specific growth rate—SGR) was shown by the probiotic-treated larval groups compared to the control. Probiotic supplementation resulted in significantly higher counts of total viable colony (TVC) and lactic acid bacteria (LAB) in the intestine. Some immunological parameters (mucosal fold fattening, goblet cell abundance, expansion of lamina propria and enterocytes) of the gut were significantly better in probiotic-treated fish. The liver of treated fish showed irregular shape nuclei turning into regular shape and reducing spaces between the hepatic cells. Probiotic-treated fish had the highest post-challenge survival rate (90%) against <em>A. veronii</em> infection. The erythrocytes of challenged fish treated with probiotics had significantly lower frequencies of various nuclear and cellular abnormalities. These findings suggest that multi-species probiotic supplements could improve the survival, growth, health status, and immune response of rohu in the early stages of its development.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001294/pdfft?md5=e67d96a3c9e25c5c59cc378525516569&pid=1-s2.0-S2772735123001294-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}