Water Biology and Security最新文献

{"title":"A high-throughput phenome-based analysis of morphological variation and environmental adaptation in extremely high-altitude schizothoracine fishes","authors":"He Gao ,&nbsp;Suxing Fu ,&nbsp;Meng Xing ,&nbsp;Yinhua Zhou ,&nbsp;Tonghan Wu ,&nbsp;Xiao Chen ,&nbsp;Chengjiang He ,&nbsp;Qiaokun Liu ,&nbsp;Haixu Liu ,&nbsp;Luo Lei ,&nbsp;Shijun Xiao ,&nbsp;Fei Liu ,&nbsp;Yan Zhou ,&nbsp;Jian Su ,&nbsp;Chaowei Zhou ,&nbsp;Bingyao Huang ,&nbsp;Haiping Liu","doi":"10.1016/j.watbs.2025.100381","DOIUrl":"10.1016/j.watbs.2025.100381","url":null,"abstract":"<div><div>Schizothoracine fishes are distributed in the Nagqu region, which is the hinterland of the Tibetan Plateau. They have adapted to the cold and strongly ultraviolet environment of the plateau and show diverse morphologies, which makes them ideal for studying the relationship between phenotype and environment. However, traditional morphological measurements are time consuming and labor costly. In this study, we propose a deep-learning-based method for acquiring high-throughput phenotypic data of fishes, including image dataset construction of schizothoracine fishes (including front, side, and top views), fish keypoint detection based on the You Only Look Once (YOLO) model, and reconstruction of 2D and 3D keypoint coordinates based on multiple views. A total of 7050 phenotypic data points consisting of keypoint distances and angles were constructed for each fish and were highly correlated (&gt;0.98) with the corresponding data measured manually. We obtained phenotypic data for a total of 525 fishes from three schizothoracine fish groups inhabiting riverine, lacustrine, and river–lake transitional environments using the proposed phenotypic data acquisition method. We employed a random forest algorithm to classify the groups, achieving a classification accuracy of 96%, and identified 15 morphometric indices that exhibited statistically significant differences., of which 6 were related to head morphology, 6 related to body shape, and 3 related to tail morphology, based on the random forest algorithm. Specifically, river-living schizothoracine fishes showed a blunt head, robust body, and elongated caudal peduncle which may reflect adaptations to the turbulence of the river, while the lake-living schizothoracine fishes have the opposite effect. Schizothoracine fishes at the river–lake transitional zones were phenotypically characterized as being in the middle of the two phenotypes, and these presumably reflect adaptations to their lake habitat. This study provides a methodological reference for obtaining high-throughput phenotypic data on fish and a theoretical basis for understanding the adaptation of very high-altitude schizothoracine fishes to their environment.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100381"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Family evolution and functional divergence of bony fish-specific Gig1 homologs","authors":"Li-Li An ,&nbsp;Xiu-Ying Gong ,&nbsp;Cheng Dan ,&nbsp;Hao-Yu Sun ,&nbsp;Wen-Hao Guo ,&nbsp;Hong-Yu Luan ,&nbsp;Meng-Yao Wu ,&nbsp;Ji-Cheng Yu ,&nbsp;Yi-Bing Zhang","doi":"10.1016/j.watbs.2025.100382","DOIUrl":"10.1016/j.watbs.2025.100382","url":null,"abstract":"<div><div>Grass carp reovirus (GCRV)-induced gene 1 (Gig1) is identified initially in crucian carp <em>C. auratus</em> as a novel interferon-stimulated gene (ISG) encoding an antiviral protein and subsequently as the founder member of Gig1 gene family. However, the evolution and function of Gig1 family members remain largely unknown. In this study, genome-wide searchs of 100 vertebrate species representing the main taxonomic lineages revealed that the Gig1 family originated from a common ancestor of bony fish but was completely lost in tetrapods. The Gig1 family is divided into three subfamilies, each with its own characteristics in gain and loss. Compared to subfamily I that originated the earliest and showed no significant change in gene copies, subfamilies II and III were expanded robustly with teleost radiation. Despite no known domains having been identified, motif prediction revealed a conserved motif arrangement in all Gig1 family proteins. Functionally, both zebrafish and grass carp have subfamilies I and II but not subfamily III. However, only certain subfamily II genes were highly induced by viral infection and thus capable of inhibiting viral replication by overexpression and knockout assays. The Japanese rice fish medaka has no subfamily II and, strikingly, one subfamily III gene acquired antiviral feature. Site-directed mutation showed that three leucine residues conserved in the motif 1 were essential for the antiviral role of subfamily II genes in zebrafish. In summary, our results indicate that bony fish-specific Gig1 gene family has been undergoing diversification in expression and function toward virus infection in a given species.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100382"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atg9a is a critical factor in the immune response of grouper (Epinephelus coioides) to viral infection","authors":"Hong Chen ,&nbsp;Zhouling Zhan ,&nbsp;Siting Wu ,&nbsp;Qiongyue Xu ,&nbsp;Helong Cao ,&nbsp;Qiwei Qin ,&nbsp;Jingguang Wei","doi":"10.1016/j.watbs.2025.100385","DOIUrl":"10.1016/j.watbs.2025.100385","url":null,"abstract":"<div><div>Autophagosome generation, development and maturation are largely dependent on Atg9a, an important member of the autophagy-related protein family. However, the potential role of fish Atg9a in viral infections is poorly understood. This research involved cloning and characterizing an Atg9a homologue from the orange-spotted grouper (<em>Epinephelus coioides</em>), known as <em>EcAtg9a</em>. The ORF (open reading frame) of <em>EcAtg9a</em> is composed of 2583 nucleotides and encodes 860 amino acids. <em>EcAtg9a</em> was detected in every tissue that was analyzed, and was particularly abundant in liver, kidney, head and blood. <em>EcAtg9a</em> expression levels in grouper spleen (GS) cells increased following infection with SGIV and RGNNV. <em>EcAtg9a</em> was uniformly present in the cytoplasm, while found to co-exist with the endoplasmic reticulum (ER), Golgi apparatus, mitochondria and lysosomes. <em>EcAtg9a</em> suppressed the expression of interferon-related genes and factors linked to inflammation while promoting the replication of SGIV as well as RGNNV in GS cells. In addition, <em>EcAtg9a</em> could interact with the key molecules of the cGAS-STING pathway including EccGAS, EcTBK1, EcSTING, and even EcIRF3. <em>EcAtg9a</em> facilitated the upregulation of Beclin1 and LC3-II synthesis within cells, resulting in an augmented formation of LC3 fluorescent aggregates. The level of eIF2α phosphorylation (S51) was increased in EcAtg9a-overexpressing cells, and the p53 protein moved from the cytoplasm into the nucleus. Findings may offer insight into how grouper's innate immunity works against viral infections.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100385"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The shared immunometabolic responses between dexamethasone-induced hepatobiliary syndrome and GCRV II-caused hemorrhagic disease reveal the pivotal role of autophagy and Hsp90 activity in metabolic and infectious diseases","authors":"Siyao Zheng ,&nbsp;Minhui Tao ,&nbsp;Xiaoman Wu ,&nbsp;Mingxian Chang","doi":"10.1016/j.watbs.2025.100400","DOIUrl":"10.1016/j.watbs.2025.100400","url":null,"abstract":"<div><div>Contamination by dexamethasone (DEX) in aquatic environments is expected to rise significantly as it is used in the treatment of inflammation, allergies, and autoimmune disorders, especially COVID-19. However, the underlying effects and mechanisms of DEX in leading to metabolic or infectious diseases have remained largely unexplored in teleosts. Here, we used zebrafish <em>(Danio rerio</em>) as a model to study the effects of DEX exposure on metabolic and infectious diseases. We found that DEX-induced hepatobiliary syndrome significantly increased susceptibility to type II grass carp reovirus (GCRV-II), which causes severe hemorrhagic disease in aquaculture. Comparative transcriptomic analysis demonstrated the shared and disease-specific immunometabolic responses among zebrafish larvae with hepatobiliary syndrome and/or GCRV-II infection. Moreover, compared with those of wild-type zebrafish, zebrafish larvae with DEX-induced hepatobiliary syndrome and/or GCRV-II infection presented increased expression of inflammatory markers (<em>il1b</em>), coagulation markers (<em>fibrinogens</em> and <em>antithrombin</em> III), and genes involved in autophagy, including <em>hsp90aa</em>. <em>In vivo</em> inhibition of autophagy via 3-MA and Hsp90 activity via geldanamycin markedly suppressed hepatic lipid deposition and reactive oxygen species accumulation caused by hepatobiliary syndrome and/or GCRV-II infection, thus significantly reducing the severity of disease and level of mortality induced by DEX and/or GCRV-II infection. In conclusion, our findings establish that the inhibition of autophagy and Hsp90 activity are promising therapeutic targets for DEX-induced hepatobiliary syndrome, GCRV-II infection, and DEX-induced hepatobiliary syndrome complicated with GCRV-II infection.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100400"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inside Front cover - Contents","authors":"","doi":"10.1016/S2772-7351(25)00120-9","DOIUrl":"10.1016/S2772-7351(25)00120-9","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100477"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From primary to secondary invasions: Strategies to mitigate the invasion risk of pikeperch","authors":"Xin Wang ,&nbsp;Zhili Pan ,&nbsp;Yahui Zhao","doi":"10.1016/j.watbs.2025.100380","DOIUrl":"10.1016/j.watbs.2025.100380","url":null,"abstract":"<div><div>Aquaculture, as a major source of protein in the food industry, heavily relies on the introduction and farming of non-native species. Pikeperch, <em>Sander lucioperca</em>, an important fish in aquaculture, poses a threat to the survival of native species because it is a piscivore and has been introduced globally. To address this issue, we examined the niche dynamics of invasive populations by comparing the climatic niches of native and invasive populations of Pikeperch. Predictive models based on global occurrence data were used to assess the distribution areas of native and invasive populations, and the model results were converted into area measurements to estimate the potential invasion risk of pikeperch. The results show that invasive populations, especially those in Asia, occupy broader climatic niches. The main difference is that, compared to native-range populations, non-natives occupy warmer and more humid regions. These climatic differences have led to niche expansion by invasive populations, with invasive populations (particularly in Asia) showing a greater potential for invasion. we applied niche modeling to evaluate the potential invasion risks associated with introducing different geographic populations. Our models provide a scientific basis for managing aquaculture introductions, aiming to reduce invasion risks and minimiz both ecological and economic impacts.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100380"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Memoriam: Professor Robert Mason Hughes","authors":"Jian-Fang Gui,&nbsp;Wei Miao,&nbsp;Rui Liu,&nbsp;Ivy Sun","doi":"10.1016/j.watbs.2025.100462","DOIUrl":"10.1016/j.watbs.2025.100462","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100462"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biologging as a potential platform for resolving ocean environmental issues and threats: Towards the development of the Internet of Animals","authors":"Takashi Iwata ,&nbsp;Tomonari Akamatsu","doi":"10.1016/j.watbs.2025.100383","DOIUrl":"10.1016/j.watbs.2025.100383","url":null,"abstract":"<div><div>Biologging is a method that utilizes animal-borne devices to study the behavioural ecology, physiology, and surrounding environment of animals. This is the Lagrangian observation method, which assigns a coordinate system origin to each animal, making the biologging unique among observation platforms. This study reviews biologging as a new tool for understanding the ocean by combining other observation methods, and proposes strategies for applying biologging technology to address various marine issues through relevant policies. Attempts to resolve marine issues using biologging are described, including ocean warming, weather forecasting, marine debris, marine chemical pollution, anthropogenic noise, preservation of marine protected areas, bycatch from fisheries, and anthropogenic disturbance of habitats used by marine top predators. While biologging alone is insufficient to resolve marine issues, it can complement other Lagrangian platforms such as drifting buoys and Eulerian remote sensing, enhancing the predictive accuracy of models, and filling existing gaps in knowledge. The Internet of Animals (IoA), which is a derivative concept of the Internet of Things (IoT), will be a future solution that collects a range of data from inaccessible ocean from animals equipped with devices through a network, making open data access crucial. Conclusively, we present research examples and potential methods involving biologging that will guide the formulation of sustainable ocean management policies.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100383"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new predictive model (MINASPACS) for spatially extensive biological assessments of southeastern Brazilian streams","authors":"Pedro Fialho Cordeiro ,&nbsp;Maria João Feio ,&nbsp;Marcos Callisto ,&nbsp;Robert M. Hughes ,&nbsp;Diego Rodrigues Macedo","doi":"10.1016/j.watbs.2025.100386","DOIUrl":"10.1016/j.watbs.2025.100386","url":null,"abstract":"<div><div>Freshwater ecosystems are threatened by flow regulation, sedimentation, habitat degradation, non-native species, and water pollution. These disturbances have led to global losses of biodiversity and habitats. Therefore, it is essential to evaluate the ecological condition of freshwater ecosystems to promote effective management practices. Quantitative predictive models based on multivariate analyses of taxa richness are recognized ecological tools that can facilitate the monitoring and managing of freshwater ecosystems worldwide. However, few studies have used this approach to assess tropical rivers and streams. By evaluating predictive models, we can assess their usefulness for determining water-body taxonomic richness. We built a RIVPACS-type model based on macroinvertebrate assemblages (MINASPACS), for spatially extensive taxa richness assessments of Minas Gerais state streams, southeast Brazil. As a second objective, we assessed the sensitivity of the MINASPACS to human-induced disturbances affecting Minas Gerais streams through the relative risk (RR) approach. The MINASPACS model was trained with biological and environmental data from 78 reference sites and showed good accuracy (<em>R</em>² ​&gt; ​0.6, SD O/E ​= ​0.16). We found that percent of urban infrastructure, percent of catchment anthropogenic land use, Turbidity, Total Nitrogen, and Total Phosphorus represented significant risks to the taxa richness of Minas Gerais streams. Because of its accuracy, sensitivity, and use of map-level predictor variables, our model provides a clear, simple, and defensible measure of stream macroinvertebrate taxa richness across diverse biomes.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100386"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification and characterization of microplastics in an intertidal gastropod the common periwinkle Littorina littorea","authors":"Mahfuzur Rahman ,&nbsp;Md Enamul Hoque ,&nbsp;Zahid Hasan ,&nbsp;Mir Md Tasnim Alam ,&nbsp;Md Jakaria ,&nbsp;Koushik Das ,&nbsp;Rangel-Buitrago Nelson ,&nbsp;Mohammad Abdul Momin Siddique","doi":"10.1016/j.watbs.2025.100401","DOIUrl":"10.1016/j.watbs.2025.100401","url":null,"abstract":"<div><div>Marine microplastics (MPs) are an increasingly important concern worldwide. This study aimed to investigate the occurrence and characteristics of microplastics in an intertidal sea snail, the common periwinkle (<em>Littorina littorea</em>) sampled from Saint Martin's Island, Bay of Bengal. The results revealed that 100% of the common periwinkle snails ingested microplastics (MPs), with a mean abundance ranging from 2.63 ​± ​1.07 to 23.89 ​± ​3.53 ​MP/g (average: 7.76 ​± ​5.77 ​MP/g). Microplastic particles in the 100–1500 ​μm size class were the most abundant (63%), whereas black and red fibers were the most abundant colors and shapes. Polypropylene (PP, 34%) and polyethylene (PE, 38%) were the major polymer types, followed by polystyrene (PS, 15%) and polyethylene terephthalate (PET, 13%). Although the polymer hazard index (PHI) showed risk categories III to IV due to highly hazardous polymers such as PS and PE in the soft tissue of the common periwinkle snails, the pollution load index (PLI &lt;10) revealed minor contamination across the sampling site of Saint Martin's Island. Gastropod feeding patterns and food items impacted MP abundance in the studied area.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 4","pages":"Article 100401"},"PeriodicalIF":4.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}