发布求助
文献互助 智能选刊 最新文献

Technical Tips Online最新文献

筛选
英文 中文
Sequential in situ hybridization on tissue sections: a novel approach for analysing co-expression of different mRNAs in a single cell 组织切片上的顺序原位杂交:一种在单个细胞中分析不同mrna共表达的新方法
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70092-8
Jean-Marc Philippe , Richard A. Pollock
{"title":"Sequential in situ hybridization on tissue sections: a novel approach for analysing co-expression of different mRNAs in a single cell","authors":"Jean-Marc Philippe , Richard A. Pollock","doi":"10.1016/S1366-2120(08)70092-8","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70092-8","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 35-39"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70092-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91759396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Efficient solubilization of proteins overproduced as inclusion bodies by use of an extreme concentration of glycerol 通过使用极高浓度的甘油有效地溶解作为包涵体过量产生的蛋白质
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70123-5
Nobuo Shimamoto , Taciana Kasciukovich , Hiroki Nagai , Richard S. Hayward
{"title":"Efficient solubilization of proteins overproduced as inclusion bodies by use of an extreme concentration of glycerol","authors":"Nobuo Shimamoto , Taciana Kasciukovich , Hiroki Nagai , Richard S. Hayward","doi":"10.1016/S1366-2120(08)70123-5","DOIUrl":"10.1016/S1366-2120(08)70123-5","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 141-143"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70123-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83144194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Addition of second primer after initial denaturation step in PCR cycling: a simple way to avoid low-molecular-weight non-specific products PCR循环初始变性步骤后添加第二引物:一种避免低分子量非特异性产物的简单方法
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70104-1
Marina Kniazeva, Jon Zawistowski, Min Han
{"title":"Addition of second primer after initial denaturation step in PCR cycling: a simple way to avoid low-molecular-weight non-specific products","authors":"Marina Kniazeva, Jon Zawistowski, Min Han","doi":"10.1016/S1366-2120(08)70104-1","DOIUrl":"10.1016/S1366-2120(08)70104-1","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 73-74"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70104-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84376684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A PCR strategy for screening DNA libraries 筛选DNA文库的PCR策略
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70096-5
Jürgen Bockmann , Carsten Winter , Michael R. Kreutz , Werner Wittkowski , Tobias M. Böckers
{"title":"A PCR strategy for screening DNA libraries","authors":"Jürgen Bockmann , Carsten Winter , Michael R. Kreutz , Werner Wittkowski , Tobias M. Böckers","doi":"10.1016/S1366-2120(08)70096-5","DOIUrl":"10.1016/S1366-2120(08)70096-5","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 48-50"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70096-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79824793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Modified gel electrophoresis loading buffer indicating addition of DNA samples 改进的凝胶电泳加载缓冲液表明添加了DNA样品
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70114-4
Stefan Kaluz, Milota Kaluzova
{"title":"Modified gel electrophoresis loading buffer indicating addition of DNA samples","authors":"Stefan Kaluz, Milota Kaluzova","doi":"10.1016/S1366-2120(08)70114-4","DOIUrl":"10.1016/S1366-2120(08)70114-4","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Page 108"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70114-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81010660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro assay for cyclin-dependent kinase activity in yeast 酵母细胞周期蛋白依赖性激酶活性的体外测定
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70083-7
C. Tennyson, V. Measday, B. Andrews
{"title":"In vitro assay for cyclin-dependent kinase activity in yeast","authors":"C. Tennyson, V. Measday, B. Andrews","doi":"10.1016/S1366-2120(08)70083-7","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70083-7","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"1 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77309750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Simplified embryonic growth medium for Caenorhabditis elegans 秀丽隐杆线虫的简化胚胎生长培养基
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70095-3
Christopher A. Shelton, Bruce Bowerman
{"title":"Simplified embryonic growth medium for Caenorhabditis elegans","authors":"Christopher A. Shelton, Bruce Bowerman","doi":"10.1016/S1366-2120(08)70095-3","DOIUrl":"10.1016/S1366-2120(08)70095-3","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 46-47"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70095-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90420425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A reliable protocol for high-quality metaphase spreads for in situ hybridization 一个可靠的方案高质量中期扩散原位杂交
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70119-3
Sylvie Michelland , Fabien Mongelard , Marie-Paule Montmasson , Michel Robert-Nicoud
{"title":"A reliable protocol for high-quality metaphase spreads for in situ hybridization","authors":"Sylvie Michelland , Fabien Mongelard , Marie-Paule Montmasson , Michel Robert-Nicoud","doi":"10.1016/S1366-2120(08)70119-3","DOIUrl":"10.1016/S1366-2120(08)70119-3","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 126-127"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70119-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81454570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of neoagaro-oligosaccharides on lipofectAMINE-mediated transfection 新琼脂寡糖对脂质体介导转染的影响
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70088-6
Naiyin Wang , Jiannis Ragoussis
{"title":"The effect of neoagaro-oligosaccharides on lipofectAMINE-mediated transfection","authors":"Naiyin Wang , Jiannis Ragoussis","doi":"10.1016/S1366-2120(08)70088-6","DOIUrl":"10.1016/S1366-2120(08)70088-6","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 23-26"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70088-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72891980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple method to obtain the 5′ ends of mRNA sequences by direct ligation of cDNA–RNA hybrids to a plasmid vector 通过将cDNA-RNA杂交体直接连接到质粒载体上获得mRNA序列5′端的简单方法
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70120-X
Charani Ranasinghe, Andrew A. Hobbs
{"title":"A simple method to obtain the 5′ ends of mRNA sequences by direct ligation of cDNA–RNA hybrids to a plasmid vector","authors":"Charani Ranasinghe, Andrew A. Hobbs","doi":"10.1016/S1366-2120(08)70120-X","DOIUrl":"10.1016/S1366-2120(08)70120-X","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 128-132"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70120-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74910383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信