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PCR-directed in vitro mutagenesis using a 'temporary' restriction site 利用“临时”限制性位点进行pcr定向体外诱变
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70097-7
Martin Spiess , Nicole Beuret
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引用次数: 1
The Sos recruitment system for identification of protein partners: a basic screening protocol 用于鉴定蛋白质伴侣的Sos招募系统:一种基本的筛选方案
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70084-9
Yael Tamir, A. Aronheim
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引用次数: 0
A green fluorescent protein tool for cell-cycle research 用于细胞周期研究的绿色荧光蛋白工具
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70085-0
Eric Vermaas , Ali Fattaey
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引用次数: 0
Avoiding adsorption of DNA to polypropylene tubes and denaturation of short DNA fragments. 避免聚丙烯管对DNA的吸附和短DNA片段的变性。
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70101-6
Claire Gaillard, François Strauss
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引用次数: 55
Isolation of RNA and analysis by Northern blotting and primer extension RNA的分离及Northern blotting和引物延伸分析
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70087-4
Tracey Mitchell, Bernard J. Morley
{"title":"Isolation of RNA and analysis by Northern blotting and primer extension","authors":"Tracey Mitchell, Bernard J. Morley","doi":"10.1016/S1366-2120(08)70087-4","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70087-4","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 20-22"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70087-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91607491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A semi-automated highly sensitive approach for mutation detection 半自动化的高灵敏度突变检测方法
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70122-3
Regine Dahse, Wolfgang Fiedler, Ulrike Riese, Ferdinand v. Eggeling, Günther Ernst
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引用次数: 1
Generation of 31-residue epitope tag insertions using transposons TnphoAlin and TnIacZIin in Escherichia coli 利用转座子TnphoAlin和TnIacZIin在大肠杆菌中插入31个残基表位标签
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70124-7
Colin Manoil
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引用次数: 0
Sequential in situ hybridization on tissue sections: a novel approach for analysing co-expression of different mRNAs in a single cell 组织切片上的顺序原位杂交:一种在单个细胞中分析不同mrna共表达的新方法
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70092-8
J. Philippe, R. A. Pollock
{"title":"Sequential in situ hybridization on tissue sections: a novel approach for analysing co-expression of different mRNAs in a single cell","authors":"J. Philippe, R. A. Pollock","doi":"10.1016/S1366-2120(08)70092-8","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70092-8","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"19 1","pages":"35-39"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87346922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Rapid screening for protein interactors using in vitro-translated protein and an expression library 利用体外翻译蛋白和表达文库快速筛选蛋白质相互作用物
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70093-X
S. Robert, R. Saint
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引用次数: 1
A green fluorescent protein tool for cell-cycle research 用于细胞周期研究的绿色荧光蛋白工具
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70085-0
Eric H. Vermaas, A. Fattaey
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引用次数: 0
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