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DNA–protein interactions detected by silver staining 银染色检测dna -蛋白相互作用
Technical Tips Online Pub Date : 2002-01-01 DOI: 10.1016/S1366-2120(08)70171-5
Marcelo Vital, Patricia Esperón
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引用次数: 1
Genotyping gene-trap mutant mice by real-time PCR 实时聚合酶链反应对基因诱捕突变小鼠进行基因分型
Technical Tips Online Pub Date : 2002-01-01 DOI: 10.1016/S1366-2120(08)70172-7
Thomas Floss, Nikolas Uez, Stefan Frey, Wolfgang Wurst
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引用次数: 1
Simple, rapid technique for cloning eukaryotic colonies 克隆真核生物菌落的简单、快速技术
Technical Tips Online Pub Date : 2002-01-01 DOI: 10.1016/S1366-2120(08)70170-3
Francois H. van der Westhuizen, Petrus J. Pretorius
{"title":"Simple, rapid technique for cloning eukaryotic colonies","authors":"Francois H. van der Westhuizen, Petrus J. Pretorius","doi":"10.1016/S1366-2120(08)70170-3","DOIUrl":"10.1016/S1366-2120(08)70170-3","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"7 1","pages":"Pages 1-2"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70170-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80495563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
High-quality CGH slides irrespective of the lymphocyte suspension used 无论使用何种淋巴细胞悬浮液，均可获得高质量的CGH切片
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70168-5
Thomas Liehr, Anita Heller, Heike Starke, Uwe Claussen
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引用次数: 1
An improved method for purifying bacterial genomic DNAs for direct sequencing by capillary automated sequencer 一种用于毛细管自动测序仪直接测序的细菌基因组dna纯化的改进方法
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70160-0
A. Wada
{"title":"An improved method for purifying bacterial genomic DNAs for direct sequencing by capillary automated sequencer","authors":"A. Wada","doi":"10.1016/S1366-2120(08)70160-0","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70160-0","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"23 1","pages":"12-14"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91079387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
An improved method for purifying bacterial genomic DNAs for direct sequencing by capillary automated sequencer 一种用于毛细管自动测序仪直接测序的细菌基因组dna纯化的改进方法
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70160-0
Akihito Wada
{"title":"An improved method for purifying bacterial genomic DNAs for direct sequencing by capillary automated sequencer","authors":"Akihito Wada","doi":"10.1016/S1366-2120(08)70160-0","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70160-0","url":null,"abstract":"<div><p>Since the first publication of the dideoxy sequencing method (Ref. <span>[1]</span>), technologies have developed for both labeling and separating molecules. Fluorescent dye labels, among others, have enabled automated sequencing with increased capacity and throughput. In addition, the enhanced sensitivity conferred by energy-transfer dyes like BigDye (PE Biosystems, Foster City, CA, USA) and DYEnamic ET (Amersham Pharmacia Biotech, Buckinghamshire, UK) raises the possibility of sequencing directly from very large plasmids such as BACs, PACs and P1s, and even from bacterial genomes (Ref. <span>[2]</span>). This would provide a means of closing gaps between contigs generated by a whole genome shotgun approach, and of identifying transposon insertion sites without any intermediate PCR step.</p><p>For successful direct sequencing, however, high-quality genomic DNA is necessary, requiring time-consuming proteinase K digestion and subsequent phenol–chloroform extraction. Here, I present a simple and reliable method for preparing bacterial genomic templates for direct sequencing. This method uses a chaotropic reagent and silica matrix for purifying needle-sheared DNA. It can be used for both Gram-positive and Gram-negative bacteria, and DNA sequences can be determined using a capillary sequencing machine [e.g. 310 Genetic Analyzer (PE Biosystems)].</p></div>","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"6 1","pages":"Pages 12-14"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70160-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91724462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Scanning for point variation in large populations by melt-MADGE 用melt-MADGE扫描大种群中的点变异
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70162-4
Emmanuel Spanakis , Mohammed Al-Dahmesh, Ian N.M. Day
{"title":"Scanning for point variation in large populations by melt-MADGE","authors":"Emmanuel Spanakis ,&nbsp;Mohammed Al-Dahmesh,&nbsp;Ian N.M. Day","doi":"10.1016/S1366-2120(08)70162-4","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70162-4","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"6 1","pages":"Pages 24-33"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70162-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91734200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved antigen detection on western blots 改进免疫印迹抗原检测
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70163-6
Hiroshi Matsugu , Takahito Nishikata
{"title":"Improved antigen detection on western blots","authors":"Hiroshi Matsugu ,&nbsp;Takahito Nishikata","doi":"10.1016/S1366-2120(08)70163-6","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70163-6","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"6 1","pages":"Pages 34-36"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70163-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90126297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Modified protocol for DNA sequence analysis using the Sequenase™ version 2.0 DNA Sequencing Kit 使用Sequenase™2.0版DNA测序试剂盒进行DNA序列分析的修改协议
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70167-3
Mickey M. Martin, Xylophone Victor, Xin Zhao, Terry S. Elton
{"title":"Modified protocol for DNA sequence analysis using the Sequenase™ version 2.0 DNA Sequencing Kit","authors":"Mickey M. Martin,&nbsp;Xylophone Victor,&nbsp;Xin Zhao,&nbsp;Terry S. Elton","doi":"10.1016/S1366-2120(08)70167-3","DOIUrl":"10.1016/S1366-2120(08)70167-3","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"6 1","pages":"Pages 46-47"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70167-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82935253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A faster procedure for preparing amniotic cells for sexing embryos 为胚胎性别鉴定准备羊膜细胞的快速程序
Technical Tips Online Pub Date : 2001-01-01 DOI: 10.1016/S1366-2120(08)70165-X
Rafael Jiménez, Francisco J. Barrionuevo, Miguel Burgos
{"title":"A faster procedure for preparing amniotic cells for sexing embryos","authors":"Rafael Jiménez,&nbsp;Francisco J. Barrionuevo,&nbsp;Miguel Burgos","doi":"10.1016/S1366-2120(08)70165-X","DOIUrl":"10.1016/S1366-2120(08)70165-X","url":null,"abstract":"<div><p>Most procedures in mammalian developmental biology involve the use of embryos from mouse or another species. In many cases, the sex of an embryo must be determined rapidly before carrying out further procedures. For instance, when several embryos of the same sex have to be pooled for RNA purification in gene-expression studies, or for tissue cultures. The time lag before the embryo tissues are used in any of these techniques is critical for accurate experimental results. The PCR-based procedures used to detect Y-linked DNA sequences or chromosome preparations are time consuming and thus clearly unsuitable for this purpose.</p></div>","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"6 1","pages":"Pages 39-40"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70165-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84491095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
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