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Coupling of optimized multiplex PCR and automated capillary electrophoresis for efficient genome-wide searches 优化多重PCR与自动毛细管电泳耦合用于高效全基因组搜索
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70100-4
Jeremy Veenstra-VanderWeele , Gregory L. Hanna , Bennett L. Leventhal , Edwin H. Cook Jr.
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引用次数: 4
Improved conditions for PTT analysis of the BRCA1, BRCA2, NF1 and APC genes 改进了BRCA1、BRCA2、NF1和APC基因的PTT分析条件
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70109-0
Kathleen Claes, Eva Machácková, Tom Callens, Greta Van der Cruyssen, Ludwine Messiaen
{"title":"Improved conditions for PTT analysis of the BRCA1, BRCA2, NF1 and APC genes","authors":"Kathleen Claes, Eva Machácková, Tom Callens, Greta Van der Cruyssen, Ludwine Messiaen","doi":"10.1016/S1366-2120(08)70109-0","DOIUrl":"10.1016/S1366-2120(08)70109-0","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 90-93"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70109-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88540223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
In situ reverse transcription–PCR in plant tissues 植物组织中的原位逆转录pcr
Technical Tips Online Pub Date : 1998-01-01 DOI: 10.1016/S1366-2120(08)70082-5
Laura Bombelli, L. Doneda, C. Tonelli, S. Dolfini
{"title":"In situ reverse transcription–PCR in plant tissues","authors":"Laura Bombelli, L. Doneda, C. Tonelli, S. Dolfini","doi":"10.1016/S1366-2120(08)70082-5","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70082-5","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"22 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78055621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A strategy to rapidly identify restriction fragment length polymorphism (RFLP) in PCR products for gene mapping in animal families 动物科基因定位PCR产物中限制性内切片段长度多态性(RFLP)的快速鉴定策略
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70112-0
H. Sunny Sun , Catherine W. Ernst , Max F. Rothschild, Christopher K. Tuggle
{"title":"A strategy to rapidly identify restriction fragment length polymorphism (RFLP) in PCR products for gene mapping in animal families","authors":"H. Sunny Sun , Catherine W. Ernst , Max F. Rothschild, Christopher K. Tuggle","doi":"10.1016/S1366-2120(08)70112-0","DOIUrl":"10.1016/S1366-2120(08)70112-0","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 102-105"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70112-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80195791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Arraying bacteriophage lambda libraries for screening by PCR 噬菌体lambda文库的排列，用于PCR筛选
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70113-2
David C. Hughes
{"title":"Arraying bacteriophage lambda libraries for screening by PCR","authors":"David C. Hughes","doi":"10.1016/S1366-2120(08)70113-2","DOIUrl":"10.1016/S1366-2120(08)70113-2","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 106-107"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70113-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85988731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Bisulfite methylation analysis of single DNA strands 单链DNA亚硫酸氢盐甲基化分析
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70086-2
Kimberly D. Tremblay
{"title":"Bisulfite methylation analysis of single DNA strands","authors":"Kimberly D. Tremblay","doi":"10.1016/S1366-2120(08)70086-2","DOIUrl":"https://doi.org/10.1016/S1366-2120(08)70086-2","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 17-19"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70086-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91759395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Direct characterization, enrichment and sequencing of differential displayed cDNA sequences 差异显示cDNA序列的直接鉴定、富集和测序
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70115-6
Jin Zeng , Mingfang Liu , Jean-Charles Fruchart
{"title":"Direct characterization, enrichment and sequencing of differential displayed cDNA sequences","authors":"Jin Zeng , Mingfang Liu , Jean-Charles Fruchart","doi":"10.1016/S1366-2120(08)70115-6","DOIUrl":"10.1016/S1366-2120(08)70115-6","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 109-110"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70115-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76658267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased in situ hybridization sensitivity using non-radioactive probes after staining for β-galactosidase activity 在β-半乳糖苷酶活性染色后，使用非放射性探针增加原位杂交敏感性
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70125-9
Denis Houzelstein , Shahragim Tajbakhsh
{"title":"Increased in situ hybridization sensitivity using non-radioactive probes after staining for β-galactosidase activity","authors":"Denis Houzelstein , Shahragim Tajbakhsh","doi":"10.1016/S1366-2120(08)70125-9","DOIUrl":"10.1016/S1366-2120(08)70125-9","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 147-149"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70125-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78063077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Efficient targeting of retrovirally FRT-tagged chromosomal loci 逆转录病毒frt标记染色体位点的有效靶向
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70118-1
Els Verhoeyen, Hansjörg Hauser, Dagmar Wirth
{"title":"Efficient targeting of retrovirally FRT-tagged chromosomal loci","authors":"Els Verhoeyen, Hansjörg Hauser, Dagmar Wirth","doi":"10.1016/S1366-2120(08)70118-1","DOIUrl":"10.1016/S1366-2120(08)70118-1","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 122-125"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70118-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88676012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol protocol 醋酸锂/单链载体DNA/聚乙二醇方案转化酿酒酵母
Technical Tips Online Pub Date : 1998-01-01 Epub Date: 2008-03-28 DOI: 10.1016/S1366-2120(08)70121-1
Ronald Agatep, Robert D. Kirkpatrick, Debra L. Parchaliuk, Robin A. Woods , R. Daniel Gietz
{"title":"Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol protocol","authors":"Ronald Agatep, Robert D. Kirkpatrick, Debra L. Parchaliuk, Robin A. Woods , R. Daniel Gietz","doi":"10.1016/S1366-2120(08)70121-1","DOIUrl":"10.1016/S1366-2120(08)70121-1","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"3 1","pages":"Pages 133-137"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70121-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91041541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 169
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