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The use of green fluorescent fusion proteins for efficient and reliable in vitro binding assays 使用绿色荧光融合蛋白进行高效可靠的体外结合试验
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70144-2
Michal Goldberg, Yosef Gruenbaum, Michael Brandeis
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引用次数: 3
Yeast two-hybrid system: part C—characterizing positives 酵母双杂交系统:c部分特征阳性
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70134-X
Debra L. Parchaliuk, Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, R. Daniel Gietz
{"title":"Yeast two-hybrid system: part C—characterizing positives","authors":"Debra L. Parchaliuk, Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, R. Daniel Gietz","doi":"10.1016/S1366-2120(08)70134-X","DOIUrl":"10.1016/S1366-2120(08)70134-X","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 35-44"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70134-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73617746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
A non-radioactive gel shift protocol enables recovery of RNA-binding proteins 非放射性凝胶转移方案使rna结合蛋白的恢复
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70143-0
Kimio J. Tanaka, Takahito Nishikata
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引用次数: 7
A useful Taq-polymerase-based method for subcloning DNA fragments with 5′ protruding ends 一种有用的基于taq聚合酶的方法，用于亚克隆具有5 '突出末端的DNA片段
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70136-3
Michael Brüss
{"title":"A useful Taq-polymerase-based method for subcloning DNA fragments with 5′ protruding ends","authors":"Michael Brüss","doi":"10.1016/S1366-2120(08)70136-3","DOIUrl":"10.1016/S1366-2120(08)70136-3","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 48-49"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70136-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78263131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and validation of a reliable internally standardized competitive RT–PCR without cloning steps 开发和验证可靠的内部标准化竞争性RT-PCR，无需克隆步骤
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70141-7
Jörg Bojunga , Ralf Kurek , Heiner Renneberg , Klaus Henning Usadel , Klaus Kusterer
{"title":"Development and validation of a reliable internally standardized competitive RT–PCR without cloning steps","authors":"Jörg Bojunga , Ralf Kurek , Heiner Renneberg , Klaus Henning Usadel , Klaus Kusterer","doi":"10.1016/S1366-2120(08)70141-7","DOIUrl":"10.1016/S1366-2120(08)70141-7","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 62-65"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70141-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90445815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A tetracycline-responsive β-galactosidase mammalian expression vector 四环素反应性β-半乳糖苷酶的哺乳动物表达载体
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70131-4
Roy R. Swiger, John A. Heddle
{"title":"A tetracycline-responsive β-galactosidase mammalian expression vector","authors":"Roy R. Swiger, John A. Heddle","doi":"10.1016/S1366-2120(08)70131-4","DOIUrl":"10.1016/S1366-2120(08)70131-4","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 21-24"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70131-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87963179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions 高效的FACS选择程序细胞经历flp介导的位点特异性转化
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70132-6
Michaela Iber, Dirk Schübeler, Jost Seibler, Maria Höxter, Jürgen Bode
{"title":"Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions","authors":"Michaela Iber, Dirk Schübeler, Jost Seibler, Maria Höxter, Jürgen Bode","doi":"10.1016/S1366-2120(08)70132-6","DOIUrl":"10.1016/S1366-2120(08)70132-6","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 25-29"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70132-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79099006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
FISH on purified extrachromosomal DNA molecules 纯化的染色体外DNA分子上的FISH
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70145-4
Theodore I. Kuschak , James T. Paul , Jim A. Wright , J. Frederic Mushinski , Sabine Mai
{"title":"FISH on purified extrachromosomal DNA molecules","authors":"Theodore I. Kuschak , James T. Paul , Jim A. Wright , J. Frederic Mushinski , Sabine Mai","doi":"10.1016/S1366-2120(08)70145-4","DOIUrl":"10.1016/S1366-2120(08)70145-4","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 75-79"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70145-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86839743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Use of B1-repeat-supplemented Cot-1 DNA to enhance background suppression in FISH 使用b1重复序列补充的Cot-1 DNA增强FISH的背景抑制
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70137-5
Allen E. Coleman, Kathleen Angkustsiri, Siegfried Janz
{"title":"Use of B1-repeat-supplemented Cot-1 DNA to enhance background suppression in FISH","authors":"Allen E. Coleman, Kathleen Angkustsiri, Siegfried Janz","doi":"10.1016/S1366-2120(08)70137-5","DOIUrl":"10.1016/S1366-2120(08)70137-5","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 50-52"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70137-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73377038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A rapid and efficient PCR approach for the generation of direct-labeled DNA probes for FISH analysis 一种快速有效的PCR方法，用于生成用于FISH分析的直接标记DNA探针
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70135-1
Erwin Petek, Klaus Wagner
{"title":"A rapid and efficient PCR approach for the generation of direct-labeled DNA probes for FISH analysis","authors":"Erwin Petek, Klaus Wagner","doi":"10.1016/S1366-2120(08)70135-1","DOIUrl":"10.1016/S1366-2120(08)70135-1","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 45-47"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70135-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73385557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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