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An optimized procedure to mutagenize long (>10 kb) plasmids by PCR PCR诱变长(>10 kb)质粒的优化方法
Technical Tips Online Pub Date : 2000-01-01 DOI: 10.1016/S1366-2120(08)70147-8
Luiz O.F. Penalva, Juan Valcárcel
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引用次数: 6
SSCP: a tool for contig building of duplicated genomic regions SSCP:一种构建重复基因组区域的工具
Technical Tips Online Pub Date : 2000-01-01 DOI: 10.1016/S1366-2120(08)70154-5
Catalina López-Correa, Hein Duhamel, Hilde Brems, Peter Marynen, Eric Legius, Joris Robert Vermeesch
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引用次数: 2
Caution when using neomycin resistance for gene targeting 使用耐新霉素基因靶向时要谨慎
Technical Tips Online Pub Date : 2000-01-01 DOI: 10.1016/S1366-2120(08)70149-1
Hassan Jumaa, Peter J. Nielsen
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引用次数: 0
Banding of mouse chromosomes after spectral karyotyping 谱核型分析后小鼠染色体的带型
Technical Tips Online Pub Date : 2000-01-01 DOI: 10.1016/S1366-2120(08)70146-6
Francis Wiener , Sabine Mai
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引用次数: 1
A strategy for the generation of RNA competitors in competitive RT–PCR 竞争性RT-PCR中产生RNA竞争者的策略
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70128-4
Sebastian Möller, Lars Edvinsson
{"title":"A strategy for the generation of RNA competitors in competitive RT–PCR","authors":"Sebastian Möller, Lars Edvinsson","doi":"10.1016/S1366-2120(08)70128-4","DOIUrl":"10.1016/S1366-2120(08)70128-4","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 4-5"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70128-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73245106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Deficiency mapping of genes in Drosophila using single-embryo PCR 用单胚PCR定位果蝇基因缺陷
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70139-9
Lee S. Hatton, Kevin OHare
{"title":"Deficiency mapping of genes in Drosophila using single-embryo PCR","authors":"Lee S. Hatton, Kevin OHare","doi":"10.1016/S1366-2120(08)70139-9","DOIUrl":"10.1016/S1366-2120(08)70139-9","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 55-57"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70139-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75390677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
A general method for direct sequencing of transposon mutants by randomly primed PCR 用随机引物PCR直接测序转座子突变体的一般方法
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70140-5
Tsute Chen, Rothsovann Yong, Hong Dong, Margaret J. Duncan
{"title":"A general method for direct sequencing of transposon mutants by randomly primed PCR","authors":"Tsute Chen, Rothsovann Yong, Hong Dong, Margaret J. Duncan","doi":"10.1016/S1366-2120(08)70140-5","DOIUrl":"10.1016/S1366-2120(08)70140-5","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 58-61"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70140-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76336604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Yeast two-hybrid system: part B—screening procedure 酵母双杂交系统:b部分筛选程序
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70133-8
Debra L. Parchaliuk, Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, R. Daniel Gietz
{"title":"Yeast two-hybrid system: part B—screening procedure","authors":"Debra L. Parchaliuk, Robert D. Kirkpatrick, Sharon L. Simon, Ronald Agatep, R. Daniel Gietz","doi":"10.1016/S1366-2120(08)70133-8","DOIUrl":"10.1016/S1366-2120(08)70133-8","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 30-34"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70133-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85201009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Sequential G-banding, SKY and FISH provide a refined identification of translocation breakpoints and complex chromosomal rearrangements 序列g带、SKY和FISH提供了易位断点和复杂染色体重排的精细鉴定
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70142-9
Jane Bayani, Ajay Pandita, Jeremy A. Squire
{"title":"Sequential G-banding, SKY and FISH provide a refined identification of translocation breakpoints and complex chromosomal rearrangements","authors":"Jane Bayani, Ajay Pandita, Jeremy A. Squire","doi":"10.1016/S1366-2120(08)70142-9","DOIUrl":"10.1016/S1366-2120(08)70142-9","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 66-69"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70142-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82949756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
A new protocol to type the Ts65Dn mouse model for Down syndrome by FISH in newborn or embryo tissue imprints 在新生儿或胚胎组织印迹中通过FISH分型唐氏综合征Ts65Dn小鼠模型的新方案
Technical Tips Online Pub Date : 1999-01-01 DOI: 10.1016/S1366-2120(08)70138-7
Marga Nadal, Xavier Estivill
{"title":"A new protocol to type the Ts65Dn mouse model for Down syndrome by FISH in newborn or embryo tissue imprints","authors":"Marga Nadal, Xavier Estivill","doi":"10.1016/S1366-2120(08)70138-7","DOIUrl":"10.1016/S1366-2120(08)70138-7","url":null,"abstract":"","PeriodicalId":101210,"journal":{"name":"Technical Tips Online","volume":"4 1","pages":"Pages 53-54"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1366-2120(08)70138-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91207789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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