Serodiagnosis and Immunotherapy in Infectious Disease最新文献_第5页

The effects of pooling serum samples from seroconverting individuals or individuals with end stage disease for HIV antibody testing: a comparison of four screen tests and three pool sizes 汇集血清转化个体或终末期疾病个体的血清样本用于艾滋病毒抗体检测的效果:四种筛选试验和三种池大小的比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80017-5

J.M. Raboud , C. Major , C. Sherlock , M.V. O'Shaughnessy

{"title":"The effects of pooling serum samples from seroconverting individuals or individuals with end stage disease for HIV antibody testing: a comparison of four screen tests and three pool sizes","authors":"J.M. Raboud , C. Major , C. Sherlock , M.V. O'Shaughnessy","doi":"10.1016/S0888-0786(96)80017-5","DOIUrl":"10.1016/S0888-0786(96)80017-5","url":null,"abstract":"<div><p>Objectives: To determine the sensitivity of four HIV screening tests when testing pooled samples from individuals with low antibody levels. Methods: Samples were obtained from 10 individuals with strong positive WB results, 11 seroconverting individuals and 8 individuals with late stage disease. Samples were tested individually and with pool sizes of 5, 10 and 20 using the BioChem test, the SYVA test, the CBC Recombigen test and Organon-Teknika's Vironostika test. Results: Samples from individuals with strong positive WB results tested positive according to all four tests at all pool sizes. One of the samples from the eight individuals with late stage disease tested negative with a pool size of 20 for all four tests and with pool sizes of 5 and greater with CBC Recombigen, SYVA and Organon-Teknika. More than half of the pools of size 20 containing samples from seroconverting individuals tested negative with SYVA, CBC Recombigen and Organon-Teknika. Sensitivity varied between 18% and 82% when samples were pooled in groups of 10 and between between 27% and 100% when samples were pooled in groups of 5. Conclusions: Pooling of samples from individuals with late-stage disease or who are in the process of seroconverting is not recommended when the results of each test are critical. When only the aggregate results are of interest, such as in anonymous seroprevalance studies, the loss in sensitivity due to pooling samples from these individuals will not qualitatively affect prevalence estimates.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 1","pages":"Pages 19-24"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80017-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84028080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Detection of antibodies to the major outer membrane protein of Chlamydia trachomatis using an in vitro transcription-translation radioimmunoprecipitation assay 利用体外转录-翻译放射免疫沉淀法检测沙眼衣原体主要外膜蛋白抗体

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80019-9

J. Verley, J. Whittum-Hudson, T. Quinn, R. Viscidi

引用次数: 0

The exclusion of recent onset toxoplasma infection in patients with prolonged IgM response by the measurement of IgA and IgG avidity 通过测定IgA和IgG的活跃性，排除IgM反应延长的新近发病的弓形虫感染

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80022-9

R.E. Holliman, G.P. Bone, J.D. Johnson

引用次数: 2

Comparison between virology and serology for the follow-up of cytomegalovirus infection in heart transplant recipients 心脏移植受者巨细胞病毒感染随访的病毒学和血清学比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80020-5

V. Ghisetti , A. Barbui , T. Lazzarotto , E. Donegani , A. Ripalti , P. Dal Monte , M. Bobbio , M. Di Summa , G. Marchiaro , M.P. Landini

{"title":"Comparison between virology and serology for the follow-up of cytomegalovirus infection in heart transplant recipients","authors":"V. Ghisetti , A. Barbui , T. Lazzarotto , E. Donegani , A. Ripalti , P. Dal Monte , M. Bobbio , M. Di Summa , G. Marchiaro , M.P. Landini","doi":"10.1016/S0888-0786(96)80020-5","DOIUrl":"10.1016/S0888-0786(96)80020-5","url":null,"abstract":"<div><p>This work aimed to evaluate serology in relation to non-quantitative polymerase chain reaction (PCR) and pp65-antigenemia for the follow-up of cytomegalovirus (CMV) infection in heart transplant recipients. Besides conventional serology, antibodies were also detected by immuno Western blotting (IWB) and by recombinant enzyme immunoassay (EIA). Twenty-five CMV infected patients were evaluated. Twelve of them experienced symptomatic infection and underwent 9-(1,3-dihydroxy-2-propoxymethyl) guanine (DHPG) therapy whereas 13 asymptomatic infections were not treated. Risk factors for developing a symptomatic infection were a high antigenemia level as well as a high and delayed IgM response to ppUL44 (p52) and a low IgG response to the virus. PCR was the most sensitive procedure for detecting CMV infection (24 out of 25 infected patients and a mean time of 40 days after transplant), followed by IWB-IgM (23 patients and 40 days) and antigenemia (22 patients and 41 days). All the 12 symptomatic infections could be detected by one of the three above-mentioned methods, whereas no single test could identify all the 13 asymptomatic infections. The combination of two tests that could detect all the 25 CMV infections was PCR plus a serological procedure (IWB-IgM or recombinant EIA for p52) and pp65-antigenemia associated with IWB-IgM. As PCR results did not correlate with the onset of CMV symptomatic infection, the present data indicate that the most rational follow-up for CMV infection in heart transplant recipients can be obtained by antigenemia and IWB-IgM.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 1","pages":"Pages 43-50"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80020-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91493769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Does Aspergillus fumigatus play a role in the disease progression from HIV to AIDS? 烟曲霉在从HIV到AIDS的疾病进展中起作用吗?

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80023-0

P.K. Bhatnagar , D. Chattopadhya , P. Usha Sarma

引用次数: 1

The evaluation of diagnostic tests for determining the Toxoplasma gondii antibody status of organ transplant donors and recipients 确定器官移植供体和受者弓形虫抗体状态诊断试验的评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80016-3

G. Hodges , J.J. Gray , A.H. Balfour , T.G. Wreghitt

引用次数: 0

Evaluation of indirect fluorescent antibody (IFA) test for kala-azar for diagnostic potential in endemic areas 黑热病流行地区间接荧光抗体(IFA)检测诊断潜力的评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80015-1

K. Mukerji, A. Puri, R. Sahai, R. Saxena, J. Srivastava, J. C. Katiyar, K. Saxena, B. Dhawan, B. B. Thakur

引用次数: 1

An evaluation of tests in routine use for the quantitation of antibody to hepatitis B surface antigen 乙型肝炎表面抗原抗体定量常规检测的评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-12-01 DOI: 10.1016/0888-0786(96)87296-9

S.C. Thompson , R.J. Warren , C.G. Ryan , D. Jolley

{"title":"An evaluation of tests in routine use for the quantitation of antibody to hepatitis B surface antigen","authors":"S.C. Thompson , R.J. Warren , C.G. Ryan , D. Jolley","doi":"10.1016/0888-0786(96)87296-9","DOIUrl":"10.1016/0888-0786(96)87296-9","url":null,"abstract":"<div><p>A quality control survey of various kits for measuring antibody to hepatitis B surface antigen (anti-HBs) was undertaken by the Serology Special Interest Group of the Australian Society of Microbiology due to concern about the performance of these tests in the field. A total of 20 panels of sera, derived from people with diverse histories with respect to hepatitis B infection and vaccination, were distributed to 19 participating laboratories using seven different commercial anti-HBs assays (representing five manufacturers) throughout Victoria. Participants were blinded with respect to replicates. Assay results were analysed to take account of the market dominance of the Abbott IMx method. In general, all tests performed adequately with respect to linearity over the range tested. Reproducibility within and between assays shows that some assays are performing inadequately in the field for quantitating anti-HBs. There was only one false positive result, from a laboratory using Amerlite, but a small number of results where a person's immune status would have been falsely reported as non-immune. Additionally, the two laboratories which used the same radioimmunoassay (RIA) test kit reported in different units, so that numerically values six to 12 times higher were reported by one laboratory compared to the other. These results underscore the need for statistically relevant independent evaluation in the absence of the licensing of kits prior to market release, and ongoing monitoring of test performance in the field, including participation in quality assurance exercises which should be regularly available.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"7 4","pages":"Pages 173-180"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(96)87296-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88479667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroepidemiology of human herpesvirus 6 infection in normal children and adults in Spain 西班牙正常儿童和成人6型人疱疹病毒感染的血清流行病学研究

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-12-01 DOI: 10.1016/0888-0786(96)87294-5

C. Roldan, J. Gutierrez, C. Maroto, G. Piedrola

{"title":"Seroepidemiology of human herpesvirus 6 infection in normal children and adults in Spain","authors":"C. Roldan, J. Gutierrez, C. Maroto, G. Piedrola","doi":"10.1016/0888-0786(96)87294-5","DOIUrl":"10.1016/0888-0786(96)87294-5","url":null,"abstract":"<div><p>We have studied the prevalence of anti-human herpesvirus 6 (HHV-6) antibodies in different population groups from Spain. Serum samples from 271 children between 0 and 15 years old, 512 intravenous drug addicts (IVDA) (262 seropositive for human immunodeficiency virus (HIV)) and 254 healthy individuals (100 pregnant women). The indirect immunofluorescence technique was used for antibody investigation from initial dilutions of 1 : 40. Seroprevalence studies showed antibody presence in 37.6%. The highest positivity was found in the group of children (49.4%, <em>P</em><0.001), followed by the pregnant women (39%), the IVDAs (34%) and healthy subjects (28%). When the IVDA group was split into HIV positive and HIV negative, no significant difference was found between them (<em>P</em> = 0.45). Antibody titres oscillated between 1 : 40 and 1 : 2560, with the greatest frequency at 1 : 40 in both male and female patients. A statistically significant difference (<em>P</em> < 0.05) was only found between sexes in the control group.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"7 4","pages":"Pages 157-160"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(96)87294-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88867478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Amplification and non-isotopic detection of specific DNA sequences in a single microtitre well 单个微滴孔中特定DNA序列的扩增和非同位素检测

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-12-01 DOI: 10.1016/0888-0786(96)87299-4

N. Tavernarakis, G. Hatzidakis, G. Vlatakis, E. Krambovitis

{"title":"Amplification and non-isotopic detection of specific DNA sequences in a single microtitre well","authors":"N. Tavernarakis, G. Hatzidakis, G. Vlatakis, E. Krambovitis","doi":"10.1016/0888-0786(96)87299-4","DOIUrl":"10.1016/0888-0786(96)87299-4","url":null,"abstract":"<div><p>We report the development of a convenient and reliable polymerase chain reaction (PCR)-based microassay for the amplification and detection of specific DNA sequences with potential applications in the diagnostics field. The major features of our system are: (a) the complete system is carried out entirely in the same microtitre well; (b) the PCR is performed in two instead of the traditional three temperatures, thus reducing the time for 35 cycles to under 2 h; (c) the probe is already immobilized onto the solid phase, allowing direct hybridization of the PCR products; (d) one of the two primers is already biotinylated at the 5' end, thus detecting one of the two actual specific products, and (e) the whole process is designed to an enzyme-linked immunosorbent assay (ELISA)-like system for easy use and takes only 3 h, rendering the system particularly suitable for a busy clinical laboratory and automation. The method was successfully applied for the detection of human immunodeficiency virus type 1 (HIV-1) from patient lymphocyte samples.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"7 4","pages":"Pages 202-206"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(96)87299-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75422816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1