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Hybrid materials from amphiphilic block copolymers and membrane proteins 由两亲嵌段共聚物和膜蛋白组成的杂化材料
Reviews in Molecular Biotechnology Pub Date : 2002-03-01 DOI: 10.1016/S1389-0352(01)00052-6
Corinne Nardin, Wolfgang Meier
{"title":"Hybrid materials from amphiphilic block copolymers and membrane proteins","authors":"Corinne Nardin,&nbsp;Wolfgang Meier","doi":"10.1016/S1389-0352(01)00052-6","DOIUrl":"10.1016/S1389-0352(01)00052-6","url":null,"abstract":"<div><p>Self-assembly of reactive amphiphilic block copolymers is used to prepare nanostructured hydrogels with exceptional permeability properties, vesicular structures and planar, freestanding membranes in aqueous solution. Although the underlying block copolymer membranes are two–three-fold thicker than conventional lipid bilayers<span>, they can be regarded as mimetic of biological membranes and can be used as a matrix for membrane-spanning proteins. Surprisingly, the proteins remain functional, despite the extreme thickness of the membranes and even after polymerization of the reactive block copolymers. The unique combination of block copolymers with membrane proteins allows the preparation of mechanically stable, defect-free membranes and nanocapsules that have highly selective permeability and/or specific recognition sites. This is documented by some representative examples.</span></p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"90 1","pages":"Pages 17-26"},"PeriodicalIF":0.0,"publicationDate":"2002-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00052-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 44
Bioelectrocatalysis by redox enzymes at modified electrodes 氧化还原酶在修饰电极上的生物电催化
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00055-1
Frieder W Scheller , Ulla Wollenberger , Chenghong Lei , Wen Jin , Bixia Ge , Claudia Lehmann , Fred Lisdat , Vadim Fridman
{"title":"Bioelectrocatalysis by redox enzymes at modified electrodes","authors":"Frieder W Scheller ,&nbsp;Ulla Wollenberger ,&nbsp;Chenghong Lei ,&nbsp;Wen Jin ,&nbsp;Bixia Ge ,&nbsp;Claudia Lehmann ,&nbsp;Fred Lisdat ,&nbsp;Vadim Fridman","doi":"10.1016/S1389-0352(01)00055-1","DOIUrl":"10.1016/S1389-0352(01)00055-1","url":null,"abstract":"<div><p><span>Self-assembled monolayers of thiolated compounds are used as promoters for protein–electrode reactions. They provide an anchor group based on thiol chemisorptions and also a functional group for effective interaction with the protein. These interactions are often governed by electrostatic attraction. For example, for positively charged proteins, such as cytochrome </span><em>c</em><span> and the selenoprotein<span><span> glutathione peroxidase, mercaptoalkanoic acids have been used. Clay modification of the electrode surface has been found to facilitate the heterogeneous </span>electron transfer<span> process for heme proteins, e.g. cytochrome </span></span></span><em>c</em><span><span><span>, cytochrome P450<span> and myoglobin. Interestingly, nucleic acids at carbon electrodes and thiol-modified double stranded </span></span>oligonucleotides act as promoters of the redox communication to proteins, whereas the mechanism is still subject to controversy interpretations. By interacting the </span>protein immobilised<span> at the electrode with species in solution, signal chains have been constructed. The interaction can result in a simple co-ordination or redox reaction, depending on the nature of the reaction partners. For analytical purposes, e.g. biosensors, the electrochemical redox conversion of the immobilised protein is evaluated.</span></span></p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 411-424"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00055-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 65
Biosensors 生物传感器
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00047-2
{"title":"Biosensors","authors":"","doi":"10.1016/S1389-0352(01)00047-2","DOIUrl":"https://doi.org/10.1016/S1389-0352(01)00047-2","url":null,"abstract":"","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Page 301"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00047-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138384242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Some new aspects in biosensors 生物传感器的几个新方面
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00048-4
Shaojun Dong, Xu Chen
{"title":"Some new aspects in biosensors","authors":"Shaojun Dong,&nbsp;Xu Chen","doi":"10.1016/S1389-0352(01)00048-4","DOIUrl":"10.1016/S1389-0352(01)00048-4","url":null,"abstract":"<div><p>This paper reviews recent advances in biosensors contributed mainly by our laboratory. The biosensors, based on the new immobilization materials — sol–gel organic–inorganic hybrid materials, cryohydrogel (or organohydrogel) and bilayer lipid membranes, are presented. The analytical performances of the biosensors are discussed. Applications of the biosensors in extreme environment are emphasized. A new generation of biosensors — surface plasmon resonance biosensors and capacitance biosensors, are also described.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 303-323"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00048-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 70
Protein modified- and membrane electrodes: strategies for the development of biomolecular sensors 蛋白质修饰电极和膜电极:生物分子传感器的发展策略
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00054-X
Pierre Bianco
{"title":"Protein modified- and membrane electrodes: strategies for the development of biomolecular sensors","authors":"Pierre Bianco","doi":"10.1016/S1389-0352(01)00054-X","DOIUrl":"10.1016/S1389-0352(01)00054-X","url":null,"abstract":"<div><p>Different procedures used for constructing protein/enzyme-modified electrodes are examined, in particular adsorption, covalent attachment and film deposition. The performances of such modified electrodes with electroactive proteins or enzymes attached to their active surface are examined, especially in the case of <em>c</em><span>-type cytochromes, hydrogenases<span> and glucose oxidase. Another strategy presented in this review consists of the use of membrane electrodes with an electroactive protein imprisoned between a dialysis membrane and the electrode surface. The versatility and other advantages of such a procedure are underlined. Applications of membrane electrodes to the bioremediation of soils and effluents and as models for investigating interactions between proteins and soils are described.</span></span></p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 393-409"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00054-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 39
Assay and screening methods for bioactive substances based on cellular signaling pathways 基于细胞信号通路的生物活性物质的测定和筛选方法
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00051-4
Yoshio Umezawa
{"title":"Assay and screening methods for bioactive substances based on cellular signaling pathways","authors":"Yoshio Umezawa","doi":"10.1016/S1389-0352(01)00051-4","DOIUrl":"10.1016/S1389-0352(01)00051-4","url":null,"abstract":"<div><p><span>Assay and screening methods for bioactive substances based on cellular signaling<span><span><span> pathways are presented. Examples include: (1) intracellular protein phosphorylation and protein–protein interaction, (1-i) a new assay method for evaluating chemical selectivity of agonists for insulin signaling pathways based on agonist-induced phosphorylation of a </span>target peptide, (1-ii) an SPR-based screening method for agonist selectivity for insulin signaling pathways based on the binding of phosphotyrosine to its specific binding protein, (1-iii) a fluorescent indicator for tyrosine phosphorylation-based insulin signaling pathways, and (1-iv) split </span>luciferase<span> as an optical probe for detecting protein–protein interactions in mammalian cells<span> based on protein splicing; (2) a screening method for antigen-specific IgE using mast cells based on intracellular calcium signaling; (3) a screening method for substrates of multidrug resistance-associated protein (MRP); and (4) fluorescent indicators for cyclic GMP based on cyclic GMP-dependent protein kinase Iα and </span></span></span></span>green fluorescent proteins.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 357-370"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00051-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation procedures 基于优化电子转移途径和非手动固定程序的安培酶生物传感器
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00058-7
Wolfgang Schuhmann
{"title":"Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation procedures","authors":"Wolfgang Schuhmann","doi":"10.1016/S1389-0352(01)00058-7","DOIUrl":"10.1016/S1389-0352(01)00058-7","url":null,"abstract":"<div><p>Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established allowing a fast electron transfer concomitantly avoiding free-diffusing redox species. Based on the specific nature of different redox proteins and non-manual immobilisation procedures possible biosensor designs are discussed, namely biosensors based on (i) direct electron transfer between redox proteins and electrodes modified with self-assembled monolayers; (ii) anisotropic orientation of redox proteins at monolayer-modified electrodes; (iii) electron-transfer cascades via redox hydrogels; and (iv) electron-transfer via conducting polymers.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 425-441"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00058-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56529374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 149
Sensor-based measurements of the role and interactions of free radicals in cellular systems 基于传感器的自由基在细胞系统中的作用和相互作用的测量
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00056-3
Calum J McNeil, Philip Manning
{"title":"Sensor-based measurements of the role and interactions of free radicals in cellular systems","authors":"Calum J McNeil,&nbsp;Philip Manning","doi":"10.1016/S1389-0352(01)00056-3","DOIUrl":"10.1016/S1389-0352(01)00056-3","url":null,"abstract":"<div><p><span>Direct real-time electrochemical measurements have offered new insight into the importance of free radical interplay in a number of cell culture and in vivo models of neurodegenerative processes. This review highlights investigations carried out in this laboratory of real-time superoxide and nitric oxide free radical generation, and presents evidence of complex inter-relationships between these species. These include: a novel function for astrocytic nitric oxide synthase in controlling neuronal nitric oxide availability; and the demonstration that extracellular superoxide flux can lead to the generation of NO by </span>glial cells. The possible consequences of these interactions are discussed.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 443-455"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00056-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Functional biosensor systems via surface-nanoengineering of electronic elements 基于电子元件表面纳米工程的功能性生物传感器系统
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00050-2
Itamar Willner, Bilha Willner, Eugenii Katz
{"title":"Functional biosensor systems via surface-nanoengineering of electronic elements","authors":"Itamar Willner,&nbsp;Bilha Willner,&nbsp;Eugenii Katz","doi":"10.1016/S1389-0352(01)00050-2","DOIUrl":"10.1016/S1389-0352(01)00050-2","url":null,"abstract":"<div><p>Bioelectronics is a progressing interdisciplinary research field that involves the integration of biomaterials with electronic transducers such as electrodes, field-effect transistors or piezoelectric crystals. Surface engineering of biomaterials such as enzymes, antigen-antibodies or DNA on the electronic supports controls the electrical properties of the biomaterial/transducer interface and enables the electronic transduction of biorecognition events, or biocatalyzed transformation, on the transducers. The development of biosensor systems of tailored sensitivites and specificities represents a major advance in bioelectronics.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 325-355"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00050-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
Electrocatalytic oxidation of NAD(P)H at mediator-modified electrodes NAD(P)H在介质修饰电极上的电催化氧化
Reviews in Molecular Biotechnology Pub Date : 2002-02-01 DOI: 10.1016/S1389-0352(01)00053-8
Lo Gorton , Elena Domı́nguez
{"title":"Electrocatalytic oxidation of NAD(P)H at mediator-modified electrodes","authors":"Lo Gorton ,&nbsp;Elena Domı&#x0301;nguez","doi":"10.1016/S1389-0352(01)00053-8","DOIUrl":"10.1016/S1389-0352(01)00053-8","url":null,"abstract":"<div><p>A review is presented dealing with electrocatalytic NADH oxidation at mediator-modified electrodes, summarising the history of the topic, as well as the present state of the art.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"82 4","pages":"Pages 371-392"},"PeriodicalIF":0.0,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(01)00053-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56528331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 217
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