Sensor-based measurements of the role and interactions of free radicals in cellular systems

Direct real-time electrochemical measurements have offered new insight into the importance of free radical interplay in a number of cell culture and in vivo models of neurodegenerative processes. This review highlights investigations carried out in this laboratory of real-time superoxide and nitric oxide free radical generation, and presents evidence of complex inter-relationships between these species. These include: a novel function for astrocytic nitric oxide synthase in controlling neuronal nitric oxide availability; and the demonstration that extracellular superoxide flux can lead to the generation of NO by glial cells. The possible consequences of these interactions are discussed.