Reviews in Molecular Biotechnology最新文献_第8页

Microbial production of spider silk proteins 蜘蛛丝蛋白的微生物生产

Reviews in Molecular Biotechnology Pub Date : 2000-08-01 DOI: 10.1016/S1389-0352(00)00008-8

Stephen R Fahnestock, Zhongjie Yao, Laura A Bedzyk

引用次数: 67

Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics RNA适体配合物与辅助因子、氨基酸、药物及氨基糖苷类抗生素的结构、识别与鉴别

Reviews in Molecular Biotechnology Pub Date : 2000-03-01 DOI: 10.1016/S1389-0352(99)00003-3

Dinshaw J Patel, Asif K Suri

引用次数: 99

Aptamers 寡核苷酸适配子

Reviews in Molecular Biotechnology Pub Date : 2000-03-01 DOI: 10.1016/S1389-0352(00)00003-9

引用次数: 0

Chemically modified nucleic acid aptamers for in vitro selections: evolving evolution 体外选择的化学修饰核酸适体:进化进化

Reviews in Molecular Biotechnology Pub Date : 2000-03-01 DOI: 10.1016/S1389-0352(99)00002-1

Wolfgang Kusser

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引用次数: 96

Aptamers as therapeutic and diagnostic agents 适体作为治疗和诊断试剂

Reviews in Molecular Biotechnology Pub Date : 2000-03-01 DOI: 10.1016/S1389-0352(99)00004-5

Edward N. Brody , Larry Gold

{"title":"Aptamers as therapeutic and diagnostic agents","authors":"Edward N. Brody , Larry Gold","doi":"10.1016/S1389-0352(99)00004-5","DOIUrl":"10.1016/S1389-0352(99)00004-5","url":null,"abstract":"<div><p>Aptamers are oligonucleotides derived from an in vitro evolution process called SELEX. Aptamers have been evolved to bind proteins which are associated with a number of disease states. Using this method, many powerful antagonists of such proteins have been found. In order for these antagonists to work in animal models of disease and in humans, it is necessary to modify the aptamers. First of all, sugar modifications of nucleoside triphosphates are necessary to render the resulting aptamers resistant to nucleases found in serum. Changing the 2′OH groups of ribose to 2′F or 2′NH<sub>2</sub> groups yields aptamers which are long lived in blood. The relatively low molecular weight of aptamers (8000–12000) leads to rapid clearance from the blood. Aptamers can be kept in the circulation from hours to days by conjugating them to higher molecular weight vehicles. When modified, conjugated aptamers are injected into animals, they inhibit physiological functions known to be associated with their target proteins. A new approach to diagnostics is also described. Aptamer arrays on solid surfaces will become available rapidly because the SELEX protocol has been successfully automated. The use of photo-cross-linkable aptamers will allow the covalent attachment of aptamers to their cognate proteins, with very low backgrounds from other proteins in body fluids. Finally, protein staining with any reagent which distinguishes functional groups of amino acids from those of nucleic acids (and the solid support) will give a direct readout of proteins on the solid support.</p></div>","PeriodicalId":101090,"journal":{"name":"Reviews in Molecular Biotechnology","volume":"74 1","pages":"Pages 5-13"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1389-0352(99)00004-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21782113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 474

In vitro selection of nucleic acids for diagnostic applications 诊断用核酸的体外选择

Reviews in Molecular Biotechnology Pub Date : 2000-03-01 DOI: 10.1016/S1389-0352(99)00005-7

Jay Hesselberth, Michael P Robertson, Sulay Jhaveri, Andrew D Ellington

引用次数: 109

Reviews in molecular biotechnology: an introductory editorial 分子生物技术综述:导论社论

Reviews in Molecular Biotechnology Pub Date : 2000-03-01 DOI: 10.1016/S1389-0352(00)00002-7

引用次数: 0