中华检验医学杂志最新文献

{"title":"Analysis of the oxidative products of DNA(8-oxo Gsn) and RNA(8-oxo dGsn) in patients with essential hypertension","authors":"Ruiping Zhang, Songlin Yu, Danchen Wang, Yutong Zou, Weiyan Zhou, Yongtong Cao, L. Qiu","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.015","url":null,"abstract":"Objective \u0000Analysis of the oxidative products of DNA and RNA in patients with hypertension by determination of 8-oxo Gsn and 8-oxo dGsn, respectively. \u0000 \u0000 \u0000Methods \u0000This is an observational study. During August and December, 2018, 139 hypertension patients without other chronic diseases with an average age of (49.6±12.4) years old, and 139 apparently healthy volunteers without hypertension with an average age of (48.5±11.7) years old were recruited. Fasting morning urine were collected. The oxidative products of DNA: 8-oxo Gsn and the products of RNA: 8-oxo dGsn were analyzed using liquid chromatography tandem mass spectrometry. Urine Cr(U-Cr), and other serum biomarkers such as ALT, Cr, UA, Glu, TG, TC were analyzed using automatic biochemical analyzers. \u0000 \u0000 \u0000Results \u0000of 8-oxo Gsn and 8-oxo dGsn were presented as median(quartile). Statistical analysis was performed using SPSS 22.0 and GraphPad Prism 5. Nonparametric test was used to compare the difference of 8-oxo Gsn and 8-oxo dGsn between the hypertension patients and the healthy controls. Results The DNA and RNA oxidative products of 8-oxo Gsn and 8-oxo dGsn in patients with hypertension and their U-Cr-corrected 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr were 14.38(10.39-19.91)ng/ml, 12.97(7.92-18.96)ng/ml, 1.10(0.88-1.38)μg/μmol and 0.96(0.75-1.30) μg/μmol, respectively, which were significantly higher than those in the healthy controls: 11.95(7.52-18.01) ng/ml, 10.12(6.42-15.04) ng/ml, 0.86(0.59-1.21) μg/μmol and 0.72(0.50-1.02) μg/μmol, respectively. After grouped by sex, 8-oxo Gsn in males, 8-oxo Gsn and 8-oxo dGsn in females showed no significant difference between patients with hypertension and healthy controls, however, after U-Cr correction, both males′ and females′ 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr in patients with hypertension were higher than that in the healthy controls. \u0000 \u0000 \u0000Conclusion \u0000The oxidative products of DNA and RNA in patients with hypertension were significantly higher than that in healthy controls. \u0000 \u0000 \u0000Key words: \u0000Hypertension; DNA; RNA; Guanosine; Deoxyguanosine; Chromatography, liquid; Tandem mass spectrometry","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"8 1","pages":"274-278"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85073205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predictive value of small densed low-density lipoprotein cholesterol and HbA1C for cardiovascular and cerebrovascular events in elderly patients with type 2 diabetes mellitus","authors":"Jingfen Lu, Jinyan Zhao, Xu Chen, Honglin Yang, Qiong Li, Fei-Ting Lu, Hua You, Hao Shen","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.011","url":null,"abstract":"Objective \u0000To investigate the serum level of low density lipoprotein cholesterol (sdLDL-C) in elderly patients (age≥65 years) with type 2 diabetes mellitus (T2DM) and the its predictive value in evaluatingthe risk of cardiovascular and cerebrovascularevents in elderly patients with T2DM. \u0000 \u0000 \u0000Methods \u0000In this retrospective study,386 elderly patients with T2DM were collected from December 2014 to December 2016, the averageage was (72.7±5.4) years old, including 269 males and 117 females; 92 of whom had cardiovascular events during follow-up, the averageage was (72.9 ± 5.2) years old, including 65 males and 27 females, and serum sdLDL-C level was detected. Meanwhile, biomarkers such as lipoprotein (a), apolipoprotein B, apolipoprotein AI and hypersensitive c-reactive protein were analyzedin all the patients.Univariate and multivariate factors were used to analyze the relationship between each variable and the occurrence of cardiovascular and cerebrovascular events. The correlation between LDL-C, sdLDL-C of all subjects with age and other lipid indexes were analyzed. ROC curve was used to determine the predictive value of sdLDL-C elevation for cardiovascular and cerebrovascular events in elderly patients with T2DM. \u0000 \u0000 \u0000Results \u0000The levels of LDL-c, sdLDL-C, non-HDL-C, GLU, HbA1c and ApoB were significantly higher in theCardio-cerebral vascular event group (t=3.26, 3.46, 2.91, 2.47, 4.03, 3.00, P<0.05). While the levels of apolipoprotein AI was significantly lower than those in theNon-cardio-cerebral vascular event group (t=-2.39, P<0.05). Cox regression analysis showed that sdLDL-C per 10 mg/dl was independently correlated with the risk of cardiovascular and cerebrovascular events(HR 1.281, 95%CI 1.225-16.032, P<0.01)after adjusted for age. SdLDL-C was positively correlated with TG, non-HDL-C and ApoB (r=0.323, 0.588, 0.623, P<0.01) and was negativelycorrelated correlation with age, HDL-C and apolipoprotein AI (r=-0.363, -0.301, P<0.01), ROC curve analysis showed that sdLDL-C had a strong ability to predict the occurrence of cardiovascular and cerebrovascular events (AUC=0.736, 95%CI 0.554 9-0.918 2, P=0.003), while HbA1c also had a strong ability to predict the occurrence of cardiovascular and cerebrovascular events(AUC=0.725, 95%CI 0.524 3-0.927 3, P=0.006). Combined sdLDL-C with HbA1had the strong ability to predict the occurrence of cardiovascular and cerebrovascular events (AUC=0.837, 95%CI 0.711 4-0.973 5, P=0.001). \u0000 \u0000 \u0000Conclusions \u0000The elevation of serum sdLDL-C in elderly patients with T2DM wasa significantly independentrisk factorof cardiovascular and cerebrovascular events. Serum sdLDL-C had a higher clinical value than LDL-C, which was expected to be the most effective predictor of lipid profile in riak assessment of cardiovascular and cerebrovascular events in elderly patients with T2DM. \u0000 \u0000 \u0000Key words: \u0000Diabetes mellitus, type 2; Cardiovascular diseases; Cholesterol, LDL; Biomarkers; Risk factors; Forecasting","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"628 1","pages":"250-254"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86686664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of element-tagged immunoassay combined with inductively coupled plasma mass spectrometry in HCG detection","authors":"Danna Pu, W. Jiang, Xinyu Wen, Guang-hong Guo, Ping Li, Chengbin Wang","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.017","url":null,"abstract":"Objective \u0000To establish an inductively coupled plasma mass spectrometry (ICP-MS) based immunoassay method for the quantitative detection of human chorionic gonadotropin (HCG), and evaluate the clinical applicability of this method. \u0000 \u0000 \u0000Methods \u0000Sm was selected as element tags, and the HCG quantitative detection system was established by double antibody sandwich method. The dosage of biotinylated antibody and reaction time were optimized. According to EP documents of Clinical and Laboratory Standards Institute (CLSI) and related standards, the analytical performance was evaluated after the establishment of the assay, including the limit of blank (LOB), linearity, precision, recovery, cross reactivity and interference test. And 88 clinical samples were measured using the new method compared to the electrochemical immunoassay (ECLIA) method. \u0000 \u0000 \u0000Results \u0000Total process completed within 30 min after optimization, and the optimal biotinylated antibody dosage was 0.5 μl. The LOB was 0.29 mIU/ml. The linearity was good within the range of 1.16-8 365.62 mIU/ml with the linear correlation coefficient greater than 0.995 (R2=0.998 0), the recovery was 97.53%-102.01%. Both intra-and inter-assay coefficients of variation of the high-value sample and the low-value sample were less than 10%. And there was no significant cross-reaction with Luteinizing hormone (LH), follicular stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). The interference bias caused by different concentrations of interference substances was less than 10%. When compared with the ECLIA method for clinical sample detection, the proposed method showed a significant correlation(R2=0.960 0). \u0000 \u0000 \u0000Conclusion \u0000The proposed ICP-MS base immunoassay for HCG detection has good accuracy, high sensitivity and specificity, and the results of analytical performance verification meet the clinical requirements, which provides experimental basis for the clinical application of this method. \u0000 \u0000 \u0000Key words: \u0000Chorionic gonadotropin; Immunoassay; Spectrophotometry, atomic; Mass spectrometry","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"1 1","pages":"284-290"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73202850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis on the performance of aldosterone testing and the results of EQA in China","authors":"Weiyan Zhou, Wenbo Luo, Qingxiang Liu, Jiangtao Zhang, Rong Ma, Cui-hua Hu, Haijian Zhao, Chuanbao Zhang","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.014","url":null,"abstract":"Objective \u0000To evaluate the performance of aldosterone testing in China through the External Quality Assessment (EQA) and improve the testing quality of aldosterone. \u0000 \u0000 \u0000Methods \u0000Two kinds of EQA program for aldosterone were carried out in China, one of which is Routine EQA and the other is Trueness verification scheme. Lyophilized sera with 5 concentration levels were used as quality control of Routine EQA. The results were grouped according to the instrument. Target values and the coefficient of variation (CV) were calculated in each group. Trueness verification scheme was verified by using frozen human sera of 3 concentration levels determined by the reference method, and the bias of each instrument group from the target value was calculated. \u0000 \u0000 \u0000Results \u0000272 laboratories submitted the testing results, and 91.6% of laboratories used chemiluminescence method. The maximum CV was obtained by radioimmunoassay and liquid chromatography mass spectrometry, and the robust CVs were 14.6%-33.4% and 43.5%-53.9%, respectively. For chemiluminescence methods, the robust group CV was less than 10%. The results of the Trueness verification scheme showed that liquid chromatography mass spectrometry method was the most accurate method, with biases of -7.9%, 8.9% and -0.7% for the three quality controls. Diasorin system had the more accurate results deviated from the target by 58.7%, 7.9% and -2.1%, respectively. The results of other chemiluminescence methods were negatively correlated with the sample concentration, and one of them with a bias of 479%. \u0000 \u0000 \u0000Conclusions \u0000The accuracy and comparability of aldosterone among laboratories in China are not satisfactory. Reagent manufacturers and laboratories should pay more attention to EQA, with the aldosterone results traceable to SI unit, and improve the test quality of aldosterone. \u0000 \u0000 \u0000Key words: \u0000Aldosterone; Quality control; Reference standards","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"6 1","pages":"267-273"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84630551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble ST2 is promising in the diagnosis of cardiovascular disease exclusion and therapy monitoring","authors":"Liying Song, Hui Yuan","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.009","url":null,"abstract":"Soluble ST2(sST2) is a decoy receptor of interleukin-33 (IL-33), which binds to the later without exerting downstream regulation. The IL-33/ST2 axis regulates several systems, relieve or aggravate the diseases. Circulating sST2 can reflect the inflammatory response to a certain extent. sST2 can be used in prognosis, monitory curative monitory and risk stratification. Here we summarize recent researches of sST2 in clinical trials and progress and highlight the future direction of the sST2 detection. More efforts and contributions should be made by laboratory medicine practitioners and doctors for widely use of sST2 in patient management and treatment decisions. \u0000 \u0000 \u0000Key words: \u0000Interleukin; Biomarkers; Heart Failure; Prognosis","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"172 1","pages":"239-244"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83536301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the influence factors in viral nucleic acid test of 2019 novel coronavirus","authors":"X. Mo, W. Qin, Q. Fu, M. Guan","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.003","url":null,"abstract":"At present, the prevention and control of new coronavirus has entered a critical period. However, the use of quantitative real-time PCR (qRT-PCR)assays for the detection of viral nucleic acid, as a crucial diagnostic approach, has been doubted in clinical practice. Herein, we have reviewed the current status of epidemic prevention and control, latest development of detection technologies, disease characteristics, clinical sampling and transport. It has also discussed the factors that may affect the performance of viral nucleic acid detection, and suggested some effective methods to improve the detection performance of the assays. \u0000 \u0000 \u0000Key words: \u0000Novel coronavirus pneumonia; RNA viruses; Molecular diagnostic techniques; Root cause analysis; Nucleic acid detection","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"49 1","pages":"213-216"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74986448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of aldosterone and harmonization of renin in China","authors":"Weiyan Zhou, Chuanbao Zhang, Wen-jun Ma, Jun Cai, Wenxiang Chen","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.010","url":null,"abstract":"Aldosterone and renin are important clinical indicators in the diagnosis and treatment of hypertension. There are two kinds of methods for aldosterone detection, including immunoassay and mass spectrometry. The coefficients of variation within the methods, as well as the deviation between methods are large.And the results of immunoassay are much higher than that of mass spectrometry. Clinically, renin tests mainly include renin concentration and renin activity, both of which have advantages and disadvantages in the diagnosis and treatment of hypertension. The disordered test results of aldosterone and renin brings great trouble to the clinical diagnosis and treatment. Standardization/consistency is urgently needed to make the test results of each laboratory more accurate and comparable. The standardization project for renin and aldosterone in China will greatly promote the standardization process in order to provide accurate and comparable clinical tests. \u0000 \u0000 \u0000Key words: \u0000Aldosterone; Renin; Reproducibility of results; Chromatography, liquid; Tandem mass spectrometry","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"25 1","pages":"245-249"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74650728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status, challenges and apportunities in cardiovascular aging mechanism and detection","authors":"Chunmin Kang, Jingjing Zhao, Xianzhang Huang","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.008","url":null,"abstract":"Age is the main risk factor for cardiovascular disease. This paper mainly discusses the mechanism of cardiovascular aging, status of detection, opportunities and challenges of research, in order to provide theoretical basis and guidance for the prevention and detection of cardiovascular aging. \u0000 \u0000 \u0000Key words: \u0000Cardiovascular diseases; Cellular senescence; Risk factors; Biomarkers","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"1 1","pages":"234-238"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77495611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of urine free and fractionated catecholamine metabolites in the diagnosis of pheochromocytoma","authors":"Kaijuan Wang, Xiaojing Gao, Wei Zhang, Xiaocui Shi, Hongying Cong, Shuming Dai, Zhou Zhou","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.012","url":null,"abstract":"Objective \u0000The diagnostic values of urine free and fractionated catecholamine metabolites (including metanephrine MN and normetanephrine NMN, usually known as MNs) were established and their clinical value was evaluated. \u0000 \u0000 \u0000Methods \u0000Using high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS), urine free MNs (f-MN, f-NMN) and fractionated MNs(t-MN, t-NMN) from 180 cases of non-pheochromocytoma and 54 cases of pheochromocytoma (PCC)patients were detected respectively.Receiver operating characteristic curve (ROC) was used to establish clinical reference cut-off value for different forms of MNs, and diagnostic efficacy of free, fractionated and total MNs was evaluated. \u0000 \u0000 \u0000Results \u0000(1) The cut-off values of f-MN, f-NMN, t-MN and t-NMN were 47.8 μg/24 h, 52.3 μg/24 h, 224.9 μg/24 h and 664 μg/24 h, respectively. The cut-off values of total f-MNs and total t-MNs were 126 μg/24 h and 1 070 μg/24 h, respectively. (2) The correlation between f-MN and t-MN (r=0.976, P 0.05). There was no significant difference in sensitivity (90.74% vs 92.59%, P>0.05), specificity (99.44% vs 96.67%, P>0.05) and AUC (0.944 vs 0.959, P>0.05) between f-NMN and t-NMN. The combined diagnostic value of MN and NMN (total MNs) was higher than MN (free type:0.932>0.579, fractionation type: 0.960>0.730), which was similar to NMN. \u0000 \u0000 \u0000Conclusions \u0000The diagnostic performance of urine free NMN or total MNs for PCC is similar to that of fractionated typewhich can meet the clinical needs.With few influencing factors, free type MNs may be used as an alternative indicator for PCC screening in the future. \u0000 \u0000 \u0000Key words: \u0000Pheochromocytoma; Catecholamines; Chromatography, high pressure liquid; Tandem mass spectrometry; Laboratory critical values; Urinalysis","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"109 1","pages":"255-260"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84891841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary establishment of reference interval for non-fasting serum triglycerides among healthy middle-aged and elderly people in some communities Beijing","authors":"Na Wang, C. Gu, Dan Xu, B. Pang, Xuemei Wu, P. Ren, Shuying Zhang, Guijian Liu, Yuliang Yuan","doi":"10.3760/CMA.J.ISSN.1009-9158.2020.03.022","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1009-9158.2020.03.022","url":null,"abstract":"Objective \u0000To establish the reference interval of serum triglyceride (TG) for 4 hours after meal in healthy middle and old people of Beijing community, and to provide the diagnostic basis for the judgment of dyslipidemia after meal. \u0000 \u0000 \u0000Methods \u0000Selected 369 elderly people from January to October 2018 in the health examination of Guang′anmen Hospital of the Chinese Academy of Traditional Chinese Medicine. The subjects collected fasting venous blood samples in the morning the next day after fasting for 12 hours, then ate a standard breakfast that conformed to the local dietary habits, and collected venous blood samples again 4 hours after eating. Serum TG levels were measured 4 h after meal using AU5822 fully automatic biochemical analyzer and matching reagents. The comparison of postprandial TG between different age and sex groups was statistically significant using the nonparametric test of two independent samples, and the comparison between postprandial and fasting TG using the nonparametric test of two paired samples with P<0.05 as the difference. The 95% confidence interval was calculated using a nonparametric method according to the relevant requirements of the CLSI EP28-A3c file, and the reference interval was expressed as P2.5, P97.5. \u0000 \u0000 \u0000Results \u0000The median 4-hour post-prandial TG of the middle-aged and elderly aged 45-59 years and those aged ≥ 60 years at health checkups were 1.65 (1.25, 2.13) mmol/L and 1.58 (1.25, 2.00) mmol/L, there was no significant difference between the two groups (Z=-1.040, P>0.05). There was no statistical difference between males 1.69 (1.22, 2.31) mmol/L and females 1.63 (1.26, 2.12) mmol/L at 4 hours postprandial TG levels in the 45-59 year-old group (Z=-0.179, P>0.05),there was also no statistical difference between 1.64 (1.22, 2.06) mmol/L for men and 1.53 (1.28, 1.99) mmol/L for women aged 60 years or older (Z=-0.256, P>0.05).Compared with the median fasting TG of 1.05 (0.87, 1.29) mmol/L, the median serum TG of 1.61 (1.25, 2.09) mmol/L at 4 hours after meal was significantly increased (Z=-16.449, P<0.01). The difference between postprandial and fasting was 0.52 (0.30, 0.85) mmol/L.The reference range of serum TG at 4 hours after meal was 0.82 to 3.02 mmol/L. \u0000 \u0000 \u0000Conclusion \u0000In this study, the reference range of serum triglycerides for 4 hours after meal was established in some healthy elderly population groups in Beijing. \u0000 \u0000 \u0000Key words: \u0000Non-fasting serum Triglyceride; Healthy population; Middle-aged and elderly people; Reference range","PeriodicalId":10096,"journal":{"name":"中华检验医学杂志","volume":"24 1","pages":"312-316"},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74039583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}