原发性高血压患者DNA(8-oxo Gsn)和RNA(8-oxo dGsn)氧化产物的分析

Q4 Health Professions

中华检验医学杂志 Pub Date : 2020-03-11 DOI:10.3760/CMA.J.ISSN.1009-9158.2020.03.015

Ruiping Zhang, Songlin Yu, Danchen Wang, Yutong Zou, Weiyan Zhou, Yongtong Cao, L. Qiu

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Urine Cr(U-Cr), and other serum biomarkers such as ALT, Cr, UA, Glu, TG, TC were analyzed using automatic biochemical analyzers. \n \n \nResults \nof 8-oxo Gsn and 8-oxo dGsn were presented as median(quartile). Statistical analysis was performed using SPSS 22.0 and GraphPad Prism 5. Nonparametric test was used to compare the difference of 8-oxo Gsn and 8-oxo dGsn between the hypertension patients and the healthy controls. Results The DNA and RNA oxidative products of 8-oxo Gsn and 8-oxo dGsn in patients with hypertension and their U-Cr-corrected 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr were 14.38(10.39-19.91)ng/ml, 12.97(7.92-18.96)ng/ml, 1.10(0.88-1.38)μg/μmol and 0.96(0.75-1.30) μg/μmol, respectively, which were significantly higher than those in the healthy controls: 11.95(7.52-18.01) ng/ml, 10.12(6.42-15.04) ng/ml, 0.86(0.59-1.21) μg/μmol and 0.72(0.50-1.02) μg/μmol, respectively. 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引用次数: 0

摘要

目的通过测定8-oxo Gsn和8-oxo dGsn，分析高血压患者DNA和RNA的氧化产物。方法观察性研究。2018年8月至12月，共招募无其他慢性疾病的高血压患者139例，平均年龄(49.6±12.4)岁，和表面健康、无高血压的志愿者139例，平均年龄(48.5±11.7)岁。收集空腹晨尿。采用液相色谱串联质谱法对DNA: 8-oxo Gsn和RNA: 8-oxo dGsn的氧化产物进行了分析。尿液Cr(U-Cr)及其他血清生物标志物如ALT、Cr、UA、Glu、TG、TC采用全自动生化分析仪进行分析。8-oxo Gsn和8-oxo dGsn的结果以中位数(四分位数)表示。采用SPSS 22.0和GraphPad Prism 5进行统计学分析。采用非参数检验比较高血压患者与健康对照组8-oxo Gsn和8-oxo dGsn的差异。结果DNA和RNA的氧化产品8-oxo内脏大神经和8-oxo dGsn高血压患者及其U-Cr-corrected 8-oxo内脏大神经/ U-Cr和8-oxo dGsn / U-Cr 14.38 ng / ml (10.39 - -19.91), 12.97 (7.92 - -18.96) ng / ml, 1.10μg /μ摩尔(0.88 - -1.38)和0.96μg /μ摩尔(0.75 - -1.30),分别是显著高于健康对照组:11.95 (7.52 - -18.01)ng / ml, 10.12 ng / ml(6.42 - -15.04), 0.86(0.59 - -1.21)μg /μ摩尔和0.72μg /μ摩尔(0.50 - -1.02),分别。按性别分组后，男性8-oxo Gsn、女性8-oxo Gsn和8-oxo dGsn在高血压患者和健康对照组之间无显著差异，但U-Cr校正后，高血压患者的8-oxo Gsn/U-Cr和8-oxo dGsn/U-Cr在男性和女性中均高于健康对照组。结论高血压患者DNA和RNA的氧化产物明显高于健康对照组。关键词:高血压;DNA;RNA;鸟嘌呤核苷;脱氧鸟苷;色谱法、液体;串联质谱法

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Analysis of the oxidative products of DNA(8-oxo Gsn) and RNA(8-oxo dGsn) in patients with essential hypertension

Objective Analysis of the oxidative products of DNA and RNA in patients with hypertension by determination of 8-oxo Gsn and 8-oxo dGsn, respectively. Methods This is an observational study. During August and December, 2018, 139 hypertension patients without other chronic diseases with an average age of (49.6±12.4) years old, and 139 apparently healthy volunteers without hypertension with an average age of (48.5±11.7) years old were recruited. Fasting morning urine were collected. The oxidative products of DNA: 8-oxo Gsn and the products of RNA: 8-oxo dGsn were analyzed using liquid chromatography tandem mass spectrometry. Urine Cr(U-Cr), and other serum biomarkers such as ALT, Cr, UA, Glu, TG, TC were analyzed using automatic biochemical analyzers. Results of 8-oxo Gsn and 8-oxo dGsn were presented as median(quartile). Statistical analysis was performed using SPSS 22.0 and GraphPad Prism 5. Nonparametric test was used to compare the difference of 8-oxo Gsn and 8-oxo dGsn between the hypertension patients and the healthy controls. Results The DNA and RNA oxidative products of 8-oxo Gsn and 8-oxo dGsn in patients with hypertension and their U-Cr-corrected 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr were 14.38(10.39-19.91)ng/ml, 12.97(7.92-18.96)ng/ml, 1.10(0.88-1.38)μg/μmol and 0.96(0.75-1.30) μg/μmol, respectively, which were significantly higher than those in the healthy controls: 11.95(7.52-18.01) ng/ml, 10.12(6.42-15.04) ng/ml, 0.86(0.59-1.21) μg/μmol and 0.72(0.50-1.02) μg/μmol, respectively. After grouped by sex, 8-oxo Gsn in males, 8-oxo Gsn and 8-oxo dGsn in females showed no significant difference between patients with hypertension and healthy controls, however, after U-Cr correction, both males′ and females′ 8-oxo Gsn/U-Cr and 8-oxo dGsn/U-Cr in patients with hypertension were higher than that in the healthy controls. Conclusion The oxidative products of DNA and RNA in patients with hypertension were significantly higher than that in healthy controls. Key words: Hypertension; DNA; RNA; Guanosine; Deoxyguanosine; Chromatography, liquid; Tandem mass spectrometry

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中华检验医学杂志 Health Professions-Medical Laboratory Technology

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