Micron and Microscopica Acta最新文献_第9页

Electron channeling contrast (ECC) imaging using specimen current 利用样品电流的电子通道对比成像(ECC)

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90127-Y

Jarle Hjelen, Bjørn-Inge Tolleshaug

引用次数: 2

Membrane receptors involved in endocytosis in rat renal proximal tubule 膜受体参与大鼠肾近端小管内吞作用

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90085-R

Erik Ilsø Christensen

引用次数: 1

Immunogold labeling in cell biology 细胞生物学中的免疫金标记

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90102-J

A.J. Verkleij

引用次数: 112

Identification of semi-coherent Y2O3 inclusions in YBa2Cu3Ox superconducting thin films by HREM HREM法鉴定YBa2Cu3Ox超导薄膜中半相干Y2O3夹杂物

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90153-5

L.R. Wallenberg, T.I. Selinder , Z. Han , U. Helmersson

引用次数: 3

Differential distribution of some elements in a camera type eye-lens as revealed by energy dispersive x-ray micro-analysis 用能量色散x射线显微分析揭示照相机式目镜中某些元素的差异分布

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90069-P

Sudip Dey

引用次数: 2

Early endocytic events 早期内吞事件

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90083-P

Steen H. Hansen

引用次数: 0

EPMXA of bulk elemental changes in the salivary gland of the blowfly (calliphora vomitoria) during metamorphosis 苍蝇(calliphora vomitoria)在变态过程中唾液腺中大量元素变化的EPMXA

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90052-F

Morgan S.M., Bowen I.D., Morgan A.J.

引用次数: 0

Point-spread sensitivity analysis for computational optical-sectioning microscopy 计算光学切片显微镜的点扩展灵敏度分析

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90024-8

Chrysanthe Preza, John M. Ollinger, James G. McNally, Lewis J. Thomas Jr.

{"title":"Point-spread sensitivity analysis for computational optical-sectioning microscopy","authors":"Chrysanthe Preza, John M. Ollinger, James G. McNally, Lewis J. Thomas Jr.","doi":"10.1016/0739-6260(92)90024-8","DOIUrl":"10.1016/0739-6260(92)90024-8","url":null,"abstract":"<div>Experimentally determined point-spread functions (PSF) have been used routinely for reconstructions of three-dimensional (3-D) microscopic objects from optical sections (Agard et al., 1989, Meth. Cell Biol., 30: 353–377; Fay et al., 1986, Opt. Meth. Cell Physiol., 40: 51–63). The microscope's PSF is usually measured by imaging a small fluorescent bead. There is a tradeoff in this measurement: very small beads are dim and bleach rapidly, while larger beads are a poorer approximation to a point source.We have simulated the effect of the bead's size on the shape of the PSF by convolving a theoretically determined PSF (of a 40 × 1.0 N.A. oil-immersion lens) with spheres of varying diameters. Simulated data were generated with a 3-D phantom and the theoretical PSF, which is defined to be the ‘true’ PSF for the simulation. Reconstructions of the phantom were obtained with each of the theoretical PSFs obtained from the beads using a regularized linear least-squares method (Preza et al., 1992, J. Opt. Soc. Am., 9: 219–228). Results show a significant drop (more than 50%) in the signal-to-noise ratio of the reconstructions for beads with diameter large than 0.22 μm. These results suggest that the bead used in the PSF measurement should have a diameter less than 30% of the diameter of the first dark ring of the infocus two-dimensional (2-D) PSF. This study quantifies the tradeoff between the quality of the reconstructions and the bead size used in the PSF measurement.</div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 501-513"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90024-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72883566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Quantitative analysis of sputtering due to ion beam bombardment of solids and biological specimens in high resolution electron microscopy 高分辨电子显微镜下固体和生物样品离子束轰击溅射的定量分析

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90072-L

Koichi Kanaya , Yoshinori Muranaka , Katsuhisa Yonehara , Koichi Adachi

{"title":"Quantitative analysis of sputtering due to ion beam bombardment of solids and biological specimens in high resolution electron microscopy","authors":"Koichi Kanaya , Yoshinori Muranaka , Katsuhisa Yonehara , Koichi Adachi","doi":"10.1016/0739-6260(92)90072-L","DOIUrl":"10.1016/0739-6260(92)90072-L","url":null,"abstract":"<div>When a positively charged ion beam is used to bombard a solid target, most of the atoms are displaced and sputtered according to the atomic sputtering theory. In the case of biological specimens, most of the bond-breaking molecules in proteins are removed, when based on the molecular sputtering theory. It was found that the thinning rate for solids and the etching rate for biological specimens, when prepared by a normal double fixation and staining method, can be measured from the sputtering yield and density of the specimens. It was also found that the thinning and etching rates depend on the removal weight per sublimation energy and bonding energy, respectively. The angular distribution of sputtering yield, its dependence on incident angle and the secondary electron emission yield were measured, and the optimum etching condition of the incidence was obtained. Experiments showed that the in situ observation of intracellular structures of biological specimens prepared by ion beam etching can be a very effective method in electron microscopy.</div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 45-64"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90072-L","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77490120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

X-ray microanalysis of ion distribution across the cortex of soybean nodules 大豆根瘤皮层离子分布的x射线显微分析

Micron and Microscopica Acta Pub Date : 1992-01-01 DOI: 10.1016/0739-6260(92)90059-M

B.J. Thomas, F.R. Minchin

引用次数: 2