Micron and Microscopica Acta最新文献_第9页

Actin filament array in an interstitial space is disorganized differently by capping agents CaCl2 and KI CaCl2和KI封盖剂对间隙内肌动蛋白丝阵列的破坏作用不同

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90086-S

Matti Järvilehto, Riitta Harjula

引用次数: 1

A study of staining for electron microscopy using collagen as a model system—IX. The effect of tannic acid fixation 以胶原蛋白为模型系统的电镜染色研究[j]。单宁酸固定的效果

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90070-T

Margaret Tzaphlidou , Christine Cummings, John A. Chapman

引用次数: 1

Immunogold labeling in cell biology 细胞生物学中的免疫金标记

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90102-J

A.J. Verkleij

引用次数: 112

The quantitation of gold-labelled macromolecules on cell surfaces by X-ray microanalysis x射线显微分析细胞表面金标记大分子的定量

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90060-Q

Non Thomas, Iolo ap Gwynn

引用次数: 1

X-ray attenuation coefficients: Current status and availability x射线衰减系数:当前状态和可用性

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90119-X

Leif Gerward

引用次数: 1

Quantitative analysis of sputtering due to ion beam bombardment of solids and biological specimens in high resolution electron microscopy 高分辨电子显微镜下固体和生物样品离子束轰击溅射的定量分析

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90072-L

Koichi Kanaya , Yoshinori Muranaka , Katsuhisa Yonehara , Koichi Adachi

{"title":"Quantitative analysis of sputtering due to ion beam bombardment of solids and biological specimens in high resolution electron microscopy","authors":"Koichi Kanaya , Yoshinori Muranaka , Katsuhisa Yonehara , Koichi Adachi","doi":"10.1016/0739-6260(92)90072-L","DOIUrl":"10.1016/0739-6260(92)90072-L","url":null,"abstract":"<div><p>When a positively charged ion beam is used to bombard a solid target, most of the atoms are displaced and sputtered according to the atomic sputtering theory. In the case of biological specimens, most of the bond-breaking molecules in proteins are removed, when based on the molecular sputtering theory. It was found that the thinning rate for solids and the etching rate for biological specimens, when prepared by a normal double fixation and staining method, can be measured from the sputtering yield and density of the specimens. It was also found that the thinning and etching rates depend on the removal weight per sublimation energy and bonding energy, respectively. The angular distribution of sputtering yield, its dependence on incident angle and the secondary electron emission yield were measured, and the optimum etching condition of the incidence was obtained. Experiments showed that the <em>in situ</em> observation of intracellular structures of biological specimens prepared by ion beam etching can be a very effective method in electron microscopy.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 45-64"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90072-L","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77490120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Computer simulated benchmarks of synthetic aperture techniques for vascular ultrasonography 血管超声合成孔径技术的计算机模拟基准

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90025-9

L. Capineri , G. Castellini , L. Masotti , S. Rocchi

{"title":"Computer simulated benchmarks of synthetic aperture techniques for vascular ultrasonography","authors":"L. Capineri , G. Castellini , L. Masotti , S. Rocchi","doi":"10.1016/0739-6260(92)90025-9","DOIUrl":"10.1016/0739-6260(92)90025-9","url":null,"abstract":"<div><p>Ultrasound investigations and related techniques strongly depend on the imaged structure: in particular our attention is focused on vascular ultrasound imaging. In this work a high-resolution imaging technique for vascular ultrasound diagnosis has been developed, with emphasis on the investigation of the carotid vessel. This application is relevant due to the stroke risk factor associated with pathological situations. Our study envisages the possibility of more accurate measurements of the lesion's extent by the application of a synthetic aperture imaging technique. An imaging system based on a standard linear array of ultrasonic transducers has been simulated, exploiting the signal acquisition from multi-offset transducers. The synthetic images are quantitatively compared with <em>in vitro</em> carotid ultrasound real images, obtained with a high resolution B-mode scanner. By an experimental procedure the actual transverse carotid sections have been obtained and the digitized video camera profiles have been used to compute the backscattered field under the weak scattering assumption. The set of synthetic radiofrequency signals has been utilized for the reconstruction of synthetic aperture images and B-mode images as well. Finally the effects due to ultrasound propagation and instrumentation characteristics have been discussed and the implications on the image quality and the carotid contour determination have been analysed.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 515-524"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90025-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80382918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Three-dimensional reconstruction of Paramecium primaurelia oral apparatus through confocal laser scanning optical microscopy 激光共聚焦扫描光学显微镜对草草虫口腔器官的三维重建

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90016-7

Francesco Beltrame , Paola Ramoino , Marco Fato , Maria Umberta Delmonte Corrado , Giampiero Marcenaro , Tina Crippa Franceschi

{"title":"Three-dimensional reconstruction of Paramecium primaurelia oral apparatus through confocal laser scanning optical microscopy","authors":"Francesco Beltrame , Paola Ramoino , Marco Fato , Maria Umberta Delmonte Corrado , Giampiero Marcenaro , Tina Crippa Franceschi","doi":"10.1016/0739-6260(92)90016-7","DOIUrl":"https://doi.org/10.1016/0739-6260(92)90016-7","url":null,"abstract":"<div><p>The oral apparatus of the ciliate protozoan <em>Paramecium primaurelia</em>, a single-celled eukaryotic organism, is a highly organized structure whose arrangement is of important taxonomic, phylogenetic and developmental significance. This paper analyses oral structures by means of a confocal laser scanning optical microscope (CLSM), which allows their three-dimensional visualization and measurement. The extraction of the intrinsic three-dimensional information related to the biological objects under investigation can in turn be related to their functional state, according to the classical paradigms of structure to function relationship identification. In our experiments, we acquired different data sets. These are optical slices of the biological sample under investigation, acquired in a confocal situation, through epi-illumination, in reflection. For comparison with conventional microscopy, two-dimensional images were acquired via a standard TV camera coupled to the microscope itself. The volumes obtained by piling up the slices were rendered through different techniques, some of them directly implemented on the workstation controlling the CLSM system, some of them on a SUN SPARCstation 1, where the original data were transferred via an Ethernet link. In this last instance, original software has been developed for the visualization and animation of the three-dimensional structures, under UNIX and X-Window, according to a ray-tracing algorithm.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 403-412"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90016-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138251149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the interface and intermediate layers of TiN/Ti/substrates systems TiN/Ti/衬底系统界面和中间层的表征

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90143-2

E.O. Ristolainen, K.A. Pischow

引用次数: 0

Point-spread sensitivity analysis for computational optical-sectioning microscopy 计算光学切片显微镜的点扩展灵敏度分析

Micron and Microscopica Acta Pub Date : 1992-01-01 Epub Date: 2002-10-09 DOI: 10.1016/0739-6260(92)90024-8

Chrysanthe Preza, John M. Ollinger, James G. McNally, Lewis J. Thomas Jr.

{"title":"Point-spread sensitivity analysis for computational optical-sectioning microscopy","authors":"Chrysanthe Preza, John M. Ollinger, James G. McNally, Lewis J. Thomas Jr.","doi":"10.1016/0739-6260(92)90024-8","DOIUrl":"10.1016/0739-6260(92)90024-8","url":null,"abstract":"<div><p>Experimentally determined point-spread functions (PSF) have been used routinely for reconstructions of three-dimensional (3-D) microscopic objects from optical sections (Agard <em>et al.</em>, 1989, <em>Meth. Cell Biol.</em>, <strong>30:</strong> 353–377; Fay <em>et al.</em>, 1986, <em>Opt. Meth. Cell Physiol.</em>, <strong>40:</strong> 51–63). The microscope's PSF is usually measured by imaging a small fluorescent bead. There is a tradeoff in this measurement: very small beads are dim and bleach rapidly, while larger beads are a poorer approximation to a point source.</p><p>We have simulated the effect of the bead's size on the shape of the PSF by convolving a theoretically determined PSF (of a 40 × 1.0 N.A. oil-immersion lens) with spheres of varying diameters. Simulated data were generated with a 3-D phantom and the theoretical PSF, which is defined to be the ‘true’ PSF for the simulation. Reconstructions of the phantom were obtained with each of the theoretical PSFs obtained from the beads using a regularized linear least-squares method (Preza <em>et al.</em>, 1992, <em>J. Opt. Soc. Am.</em>, <strong>9:</strong> 219–228). Results show a significant drop (more than 50%) in the signal-to-noise ratio of the reconstructions for beads with diameter large than 0.22 μm. These results suggest that the bead used in the PSF measurement should have a diameter less than 30% of the diameter of the first dark ring of the infocus two-dimensional (2-D) PSF. This study quantifies the tradeoff between the quality of the reconstructions and the bead size used in the PSF measurement.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 501-513"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90024-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72883566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9