{"title":"YBa2Cu3O7-δ thin films on Si substrates with Y2O3 and (Zr1-xYx)O2 buffer layers. The YBa2Cu3O7-δ-Y2O3 and Y2O3-(Zr1-xYx)O2 interfaces.","authors":"Asgeir Bardal , Markus Zwerger , Oliver Eibl","doi":"10.1016/0739-6260(92)90107-O","DOIUrl":"10.1016/0739-6260(92)90107-O","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 139-140"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90107-O","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74773338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantitation of immunogold labeling","authors":"M.A. Hayat","doi":"10.1016/0739-6260(92)90068-O","DOIUrl":"10.1016/0739-6260(92)90068-O","url":null,"abstract":"<div><p>Most of the published, quantitative immunogold labeling studies do not reflect the true concentration of antigen in a given cell compartment. Presently, the majority of the data on labeling density provides a potential way to follow the distribution of antigens, but does not indicate the quantitative concentration of the antigen present. The majority of the published quantitative data is semiquantitative. Ideal quantitative data must relate the intensity of the immunoreaction to the concentration of the underlying antigen population. Since this requirement is not fulfilled by most of the available methods, caution is warranted in interpreting the immunogold quantitative data. The difficulty in obtaining reproducible quantitative data is also due to the fact that preparatory procedures are quantitatively unknown parameters with respect to quantitative immunogold postembedding and preembedding labeling.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 1-16"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90068-O","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88862043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Badrinath Roysam , Anoop K. Bhattacharjya , Chukka Srinivas , Donald H. Szarowski , James N. Turner
{"title":"Unsupervised noise removal algorithms for three-dimensional confocal fluorescence microscopy","authors":"Badrinath Roysam , Anoop K. Bhattacharjya , Chukka Srinivas , Donald H. Szarowski , James N. Turner","doi":"10.1016/0739-6260(92)90020-E","DOIUrl":"10.1016/0739-6260(92)90020-E","url":null,"abstract":"<div><p>Algorithms are presented for effective suppression of the quantum noise artifact that is inherent to three-dimensional confocal fluorescence microscopy images of extended spatial objects such as neurons. The specific advances embodied in these algorithms are as follows: (i) they incorporate an automatic and pattern-constrained three-dimensional segmentation of the image field, and use it to limit any smoothing to the interiors of specified image regions and hence avoid the blurring that is inevitably associated with conventional noise removal algorithms; (ii) they are ‘unsupervised’ in the sense that they automatically estimate and adapt to the unknown spatially and temporally varying noise level in the microscopy data. Fast computation is achieved by parallel computation methods, rather than by algorithmic or modelling compromises.</p><p>The quantum noise artifact is modelled using a mixture of spatially non-homogeneous Poisson point processes. The intensity of each component process is constrained to lie in specific intervals. A set of segmentation and edge-site variables are used to determine the intensity of the mixture process. Using this model, the noise-removal process is formulated as the joint optimal estimation of the segmentation labels, edge-sites and intensity of the mixture Poisson point process, subject to a combination of stochastic priors and syntactic pattern constraints. The computations proceed iteratively, starting from a set of approximate user-supplied, or default initial guesses of the underlying random process parameters. An Expectation Maximization algorithm is used to obtain a more precise characterization of these parameters, that are then input to a joint estimation algorithm.</p><p>Stereoscopic renderings of processed three-dimensional images of murine hippocampal neurons are presented to demonstrate the effectiveness of the method. The processed images exhibit increased contrast and significant smoothing and reduction of the background intensity while avoiding any blurring of the foreground neuronal structures.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 447-461"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90020-E","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74813197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Membrane receptors involved in endocytosis in rat renal proximal tubule","authors":"Erik Ilsø Christensen","doi":"10.1016/0739-6260(92)90085-R","DOIUrl":"10.1016/0739-6260(92)90085-R","url":null,"abstract":"<div><p>In conclusion the present study has demonstrated the localization of 3 receptors for endocytosis in rat renal proximal tubule cells. The three receptors are located in the membranes of the vacuolar compartment involved in endocytosis in these cells and in addition in dense apical tubules responsible for membrane recycling in the proximal tubule.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 93-94"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90085-R","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85893552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Campos, J.A. López-Escámez, F.J. Cañizares, P.V. Crespo
{"title":"Electron probe X-ray microanalysis of Ca and K distributions in the otolithic membrane","authors":"A. Campos, J.A. López-Escámez, F.J. Cañizares, P.V. Crespo","doi":"10.1016/0739-6260(92)90039-G","DOIUrl":"10.1016/0739-6260(92)90039-G","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 349-350"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90039-G","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77070119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TEM investigation of an ion implanted steel","authors":"K.E. Mogensen, K. Glejbøl","doi":"10.1016/0739-6260(92)90138-4","DOIUrl":"10.1016/0739-6260(92)90138-4","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 201-202"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90138-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77144076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An unusual type of corneal nipple in the earwig, forficula sp., with a possible anti-reflection role","authors":"Sudip Dey, Begonia Dkhar","doi":"10.1016/0739-6260(92)90035-C","DOIUrl":"10.1016/0739-6260(92)90035-C","url":null,"abstract":"<div><p>An unusual type of corneal nipple pattern, with features different from the usual lower height (Group I) and greater height (Group II and III) nipples, has been detected in the corneal surface of the earwig, <em>Forficula</em> sp. However, the nipples show some similarities with each of the two above types possessing certain characters. The height of the nipples, their shape and arrangement in the facet suggest that the nipples may act as an anti-reflection device, and thus increase the intensity of transmitted light, thereby aiding scotopic vision of the insect.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 337-339"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90035-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85678482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}