{"title":"Feature extraction from three-dimensional images in quantitative microscopy","authors":"Steven S.S. Poon , Rabab K. Ward , Branko Palcic","doi":"10.1016/0739-6260(92)90022-6","DOIUrl":"10.1016/0739-6260(92)90022-6","url":null,"abstract":"<div><p>Different methods are investigated in selecting and generating the appropriate microscope images for analysis of three-dimensional objects in quantitative microscopy. Traditionally, the ‘best’ focused image from a set is used for quantitative analysis. Such an objectively determined image is optimal for the extraction of some features, but may not be the best image for the extraction of all features. Various methods using multiple images are here developed to obtain a tighter distribution for all features.</p><p>Three different approaches for analysis of images of stained cervical cells were analyzed. In the first approach, features are extracted from each image in the set. The feature values are then averaged to give the final result. In the second approach, a set of varying focused images are reconstructed to obtain a set of in-focus images. Features are then extracted from this set and averaged. In the third approach, a set of images in the three-dimensional scene is compressed into a single two-dimensional image. Four different compression methods are used. Features are then extracted from the resulting two-dimensional image. The third approach is employed on both the raw and transformed images.</p><p>Each approach has its advantages and disadvantages. The first approach is fast and produces reasonable results. The second approach is more computationally expensive but produces the best results. The last approach overcomes the memory storage problem of the first two approaches since the set of images is compressed into one. The method of compression using the highest gradient pixel produces better results overall than other data reduction techniques and produces results comparable to the first approach.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 481-489"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90022-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77901758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Limitations on cell volume determination using three-dimensional optical microscopy","authors":"D.F. Abbott, K.A. Nugent","doi":"10.1016/0739-6260(92)90023-7","DOIUrl":"10.1016/0739-6260(92)90023-7","url":null,"abstract":"<div><p>One important application for three-dimensional microscopy is the determination of the volumes of biological cells. In this paper we investigate the application of three-dimensional deconvolution techniques to the problem of cell volume determination. The theoretical discussion is based on a Fourier sampling condition for reliable image reconstruction and it is shown, using this condition and simulated images, that the cell volume determination is reliable only for cells with a shape and orientation such that their length along the optic axis is much greater than their width.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 491-500"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90023-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90865803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HREM study of zeolite Na-Y","authors":"Viveka Alfredsson","doi":"10.1016/0739-6260(92)90104-L","DOIUrl":"10.1016/0739-6260(92)90104-L","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 133-134"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90104-L","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80809681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High-resolution imaging with a coherent electron source: The field emission gun","authors":"Judith M. Brock , Max T. Otten , Wim M. Coene","doi":"10.1016/0739-6260(92)90112-Q","DOIUrl":"10.1016/0739-6260(92)90112-Q","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 149-150"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90112-Q","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74755970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Freeze-drying and cryosputtering with tungsten or niobium for high resolution scanning electron microscopy","authors":"Bengt-Arne Fredriksson, Margaretha Lindroth","doi":"10.1016/0739-6260(92)90080-W","DOIUrl":"10.1016/0739-6260(92)90080-W","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 83-84"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90080-W","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73773279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"X-raymicroanalysis: Comparison of mineral deposits in Alzheimer's disease and control tissue using TEM","authors":"S.K. Shinghrao , J.W. Neal , G. Cole , G.R. Newman","doi":"10.1016/0739-6260(92)90057-K","DOIUrl":"10.1016/0739-6260(92)90057-K","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 385-386"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90057-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77163413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vidar Hansen , Helene Seim , Helmer Fjellvåg , Arne Olsen
{"title":"Electron microscopy study of some Ni-S phases","authors":"Vidar Hansen , Helene Seim , Helmer Fjellvåg , Arne Olsen","doi":"10.1016/0739-6260(92)90126-X","DOIUrl":"10.1016/0739-6260(92)90126-X","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 177-178"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90126-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83714204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SEM-EDXA characterisation of metal impregnated cuticle","authors":"Simon A. Smith , Paul Wyeth , Julian F.V. Vincent","doi":"10.1016/0739-6260(92)90058-L","DOIUrl":"10.1016/0739-6260(92)90058-L","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 387-388"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90058-L","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91536405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The benefits of real time spectrum processing in quantitative X-ray microanalysis","authors":"Janet Wood","doi":"10.1016/0739-6260(92)90061-H","DOIUrl":"10.1016/0739-6260(92)90061-H","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 393-394"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90061-H","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76894792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}