{"title":"三维光学显微镜测定细胞体积的局限性","authors":"D.F. Abbott, K.A. Nugent","doi":"10.1016/0739-6260(92)90023-7","DOIUrl":null,"url":null,"abstract":"<div><p>One important application for three-dimensional microscopy is the determination of the volumes of biological cells. In this paper we investigate the application of three-dimensional deconvolution techniques to the problem of cell volume determination. The theoretical discussion is based on a Fourier sampling condition for reliable image reconstruction and it is shown, using this condition and simulated images, that the cell volume determination is reliable only for cells with a shape and orientation such that their length along the optic axis is much greater than their width.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 491-500"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90023-7","citationCount":"1","resultStr":"{\"title\":\"Limitations on cell volume determination using three-dimensional optical microscopy\",\"authors\":\"D.F. Abbott, K.A. Nugent\",\"doi\":\"10.1016/0739-6260(92)90023-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>One important application for three-dimensional microscopy is the determination of the volumes of biological cells. In this paper we investigate the application of three-dimensional deconvolution techniques to the problem of cell volume determination. The theoretical discussion is based on a Fourier sampling condition for reliable image reconstruction and it is shown, using this condition and simulated images, that the cell volume determination is reliable only for cells with a shape and orientation such that their length along the optic axis is much greater than their width.</p></div>\",\"PeriodicalId\":100925,\"journal\":{\"name\":\"Micron and Microscopica Acta\",\"volume\":\"23 4\",\"pages\":\"Pages 491-500\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0739-6260(92)90023-7\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Micron and Microscopica Acta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0739626092900237\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Micron and Microscopica Acta","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0739626092900237","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Limitations on cell volume determination using three-dimensional optical microscopy
One important application for three-dimensional microscopy is the determination of the volumes of biological cells. In this paper we investigate the application of three-dimensional deconvolution techniques to the problem of cell volume determination. The theoretical discussion is based on a Fourier sampling condition for reliable image reconstruction and it is shown, using this condition and simulated images, that the cell volume determination is reliable only for cells with a shape and orientation such that their length along the optic axis is much greater than their width.
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