{"title":"Confocal microscopy via reciprocal optical fibre image detection","authors":"T. Wilson, F. Reinholz","doi":"10.1016/0739-6260(92)90018-9","DOIUrl":"10.1016/0739-6260(92)90018-9","url":null,"abstract":"<div><p>We describe a compact form of confocal scanning microscope using a semiconductor laser. Confocal operation is ensured by the use of a single mode optical fibre for both launching the light into the microscope and collecting the signal from the object. The collected light is allowed to re-enter the laser and the image is detected as a modulation on the signal from the laser power monitor diode. Images are compared with those obtained from traditional point detectors. The alignment tolerances of the reciprocal scheme are found to be greatly reduced over conventional confocal systems.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 429-435"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90018-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72567065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular biology in living cells by means of digital optical microscopy","authors":"Paolo Gualtieri","doi":"10.1016/0739-6260(92)90028-C","DOIUrl":"10.1016/0739-6260(92)90028-C","url":null,"abstract":"<div><p>The intention of this review is to introduce to microscopists some of the fundamental limits as well as the advances made in digital optical microscopy techniques, also to show their potentialities in the field of molecular cell biology. Several items within the wide field of this subject will be discussed, such as geometric and photometric features, photophysics, statistics of photon flux and detection, spatial and intensity characteristics of digital images, their manipulation and display, fluorescent probes, and measuring techniques.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 239-257"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90028-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82549979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seth R. Goldstein , Thomas Hubin , Thomas G. Smith Jr.
{"title":"An improved no-moving-parts video-rate confocal microscope","authors":"Seth R. Goldstein , Thomas Hubin , Thomas G. Smith Jr.","doi":"10.1016/0739-6260(92)90019-A","DOIUrl":"10.1016/0739-6260(92)90019-A","url":null,"abstract":"<div><p>Several years ago our research program developed a video-rate confocal microscope with no moving parts, based on synchronizing and aligning the scan of an image dissector tube (IDT) with the light returning from a microscope stage that has been acousto-optically scanned by a laser beam. Improvements on the original system have recently been completed. The laser power has been substantially increased and the laser scan is now brought into the Nikon Diaphot inverted microscope through the epi-illumination port. Aberrations in the scanned beam have been reduced by performing the beam shaping required by the acousto-optic deflectors using prisms instead of cylindrical lenses. The IDT is located at the side camera output port at the end of a simple, efficient light path. The new system is described in detail and results obtained using the microscope in reflection and fluorescence mode are presented.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 437-446"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90019-A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74392533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of tunicamycin on autophagy in cultured rat fibroblasts","authors":"Eeva-Liisa Punnonen, Soile Autio, Hilkka Reunanen","doi":"10.1016/0739-6260(92)90097-W","DOIUrl":"10.1016/0739-6260(92)90097-W","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 117-118"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90097-W","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87877843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A sensitive way to determine the thickness of thin carbon films by SEM","authors":"M.D. Bentzon , S.S. Eskildsen , P.S. Nielsen","doi":"10.1016/0739-6260(92)90108-P","DOIUrl":"10.1016/0739-6260(92)90108-P","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 141-142"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90108-P","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90382822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ernst-Rudolf Krefting, Kai Frentzel, Jörg Teβarek, Hans-Jürgen Hohling
{"title":"Quantitative electronprobe X-ray microanalysis on the calcium and phosphorus distribution and calcium transport in epiphyseal growth plate","authors":"Ernst-Rudolf Krefting, Kai Frentzel, Jörg Teβarek, Hans-Jürgen Hohling","doi":"10.1016/0739-6260(92)90047-H","DOIUrl":"10.1016/0739-6260(92)90047-H","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 365-366"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90047-H","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90489872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Routing of brushborder and lysosomal enzymes in polarized epithelial cells (Review)","authors":"Leo A. Ginsel, Jack A.M. Fransen","doi":"10.1016/0739-6260(92)90081-N","DOIUrl":"10.1016/0739-6260(92)90081-N","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 85-86"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90081-N","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73302125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gert H. Hansen, Lise-Lotte Wetterberg, Mona D. Poulsen, Ove Norén, Hans Sjöström
{"title":"Microvillus membrane enzymes along the crypt-villus axis","authors":"Gert H. Hansen, Lise-Lotte Wetterberg, Mona D. Poulsen, Ove Norén, Hans Sjöström","doi":"10.1016/0739-6260(92)90082-O","DOIUrl":"10.1016/0739-6260(92)90082-O","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Page 87"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90082-O","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78917542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}