{"title":"Feature extraction from three-dimensional images in quantitative microscopy","authors":"Steven S.S. Poon , Rabab K. Ward , Branko Palcic","doi":"10.1016/0739-6260(92)90022-6","DOIUrl":"10.1016/0739-6260(92)90022-6","url":null,"abstract":"<div><p>Different methods are investigated in selecting and generating the appropriate microscope images for analysis of three-dimensional objects in quantitative microscopy. Traditionally, the ‘best’ focused image from a set is used for quantitative analysis. Such an objectively determined image is optimal for the extraction of some features, but may not be the best image for the extraction of all features. Various methods using multiple images are here developed to obtain a tighter distribution for all features.</p><p>Three different approaches for analysis of images of stained cervical cells were analyzed. In the first approach, features are extracted from each image in the set. The feature values are then averaged to give the final result. In the second approach, a set of varying focused images are reconstructed to obtain a set of in-focus images. Features are then extracted from this set and averaged. In the third approach, a set of images in the three-dimensional scene is compressed into a single two-dimensional image. Four different compression methods are used. Features are then extracted from the resulting two-dimensional image. The third approach is employed on both the raw and transformed images.</p><p>Each approach has its advantages and disadvantages. The first approach is fast and produces reasonable results. The second approach is more computationally expensive but produces the best results. The last approach overcomes the memory storage problem of the first two approaches since the set of images is compressed into one. The method of compression using the highest gradient pixel produces better results overall than other data reduction techniques and produces results comparable to the first approach.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 481-489"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90022-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77901758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Schmidt, K. Hjemsted, E. Johnson, K.K. Bourdelle , A. Johansen, H.H. Andersen, L. Sarholt-Kristensen
{"title":"Size distributions of In and Pb inclusions in Al","authors":"B. Schmidt, K. Hjemsted, E. Johnson, K.K. Bourdelle , A. Johansen, H.H. Andersen, L. Sarholt-Kristensen","doi":"10.1016/0739-6260(92)90145-4","DOIUrl":"10.1016/0739-6260(92)90145-4","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 215-216"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90145-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78103704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Intermediate structures and crystalline approximants in the I→α transformation of Al-(Mn,Fe)-Si alloys","authors":"V. Hansen, J. Gjønnes","doi":"10.1016/0739-6260(92)90125-W","DOIUrl":"10.1016/0739-6260(92)90125-W","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 175-176"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90125-W","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74146062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Confocal microscopy via reciprocal optical fibre image detection","authors":"T. Wilson, F. Reinholz","doi":"10.1016/0739-6260(92)90018-9","DOIUrl":"10.1016/0739-6260(92)90018-9","url":null,"abstract":"<div><p>We describe a compact form of confocal scanning microscope using a semiconductor laser. Confocal operation is ensured by the use of a single mode optical fibre for both launching the light into the microscope and collecting the signal from the object. The collected light is allowed to re-enter the laser and the image is detected as a modulation on the signal from the laser power monitor diode. Images are compared with those obtained from traditional point detectors. The alignment tolerances of the reciprocal scheme are found to be greatly reduced over conventional confocal systems.</p></div>","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 4","pages":"Pages 429-435"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90018-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72567065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jan-Olov Bovin, Anna Carlsson, Jan-Olle Malm, Günther Schmid
{"title":"On the structure and temperature stability of ligand covered, 18 nm, gold colloids","authors":"Jan-Olov Bovin, Anna Carlsson, Jan-Olle Malm, Günther Schmid","doi":"10.1016/0739-6260(92)90111-P","DOIUrl":"10.1016/0739-6260(92)90111-P","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 147-148"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90111-P","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75267904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Backscattered electron imaging, X-ray microanalysis and X-ray microscopy in the study of cell death","authors":"Ivor D. Bowen, Kate Mullarkey, Nicola Worrill","doi":"10.1016/0739-6260(92)90038-F","DOIUrl":"10.1016/0739-6260(92)90038-F","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 3","pages":"Pages 347-348"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90038-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73795381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Routing of brushborder and lysosomal enzymes in polarized epithelial cells (Review)","authors":"Leo A. Ginsel, Jack A.M. Fransen","doi":"10.1016/0739-6260(92)90081-N","DOIUrl":"10.1016/0739-6260(92)90081-N","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 85-86"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90081-N","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73302125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HREM investigations of two minerals, Cu3(As,Sb)S4","authors":"Mihály Pósfai , Margareta Sundberg","doi":"10.1016/0739-6260(92)90141-Y","DOIUrl":"10.1016/0739-6260(92)90141-Y","url":null,"abstract":"","PeriodicalId":100925,"journal":{"name":"Micron and Microscopica Acta","volume":"23 1","pages":"Pages 207-208"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0739-6260(92)90141-Y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91161653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}