临床检验杂志最新文献_第4页

临床检验杂志 Pub Date : 2018-01-01 DOI: 10.15324/KJCLS.2018.50.1.1

Chang-Eun Park

引用次数: 0

Effect of Fucoidan on Angiogenesis and Gene Expression in Human Umbilical Vein Endothelial Cells 岩藻多糖对人脐静脉内皮细胞血管生成及基因表达的影响

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.323

Ho Park, Beom-Su Kim

{"title":"Effect of Fucoidan on Angiogenesis and Gene Expression in Human Umbilical Vein Endothelial Cells","authors":"Ho Park, Beom-Su Kim","doi":"10.15324/KJCLS.2017.49.4.323","DOIUrl":"https://doi.org/10.15324/KJCLS.2017.49.4.323","url":null,"abstract":"신생혈관생성은 여러 신생혈관 생성 인자들이 포함되는 중요한 과정이며, 특히 이 과정에서는 섬유아세포증식인자(FGF-2)는 세포의 증식률과 미세관 형성을 촉진하기 때문에 중요한 신생혈관 생성인자로 여겨진다. 최근 연구에 따르면 해조류에서 추출되는 후코이단 다당류 물질이 섬유아세포 증식인자2에 의한 혈관내피세포의 미세관형성을 더욱 촉진한다고 보고하였다. 그러나 섬유아세포 증식인자와 후코이단 복합처리에 따른 신생혈관생성 활성에 대한 분자적 메카니즘은 아직 연구가 부족하다. 따라서 본 연구에서는 신생혈관생성 활성을 알아보기 위하여 섬유아세포 증식인자와 후코이단 물질의 복합처리에 따른 세포의 증식과 미세관형성률 그리고 세포의 이동율을 측정하였다. 또한 이들의 신생혈관 생성 활성에 관련된 인자를 탐색하기 위하여 VEGF-A, ICAM-1, MMP9, 그리고 ICAM-1 유전자를 연전사 중합연쇄반응으로 평가하였다. 본 연구의 결과에서는 후코이단과 섬유아세포 증식인자 복합처리는 혈관내피세포의 성장률, 미세관 형성률 그리고 세포의 이동률을 촉진하고, 이 과정에서 신생혈관생성 기능과 관련된 STAT3, VEGF-A, MMP9 그리고 ICAM-1의 유전자 발현을 촉진함으로 신생혈관생성활성이 나타나는 것으로 보여진다. 그러나 이러한 유전자 발현이 fucoidan/FGF2에 의한 angiogenic 활성 촉진에 직접적인 영향을 미치는 지에 대한 추가적인 연구가 이루어져야 할 것으로 생각된다.","PeriodicalId":10080,"journal":{"name":"临床检验杂志","volume":"107 1","pages":"323-328"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78653651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer miR-23b与miR-203在子宫内膜癌中的表达比较

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.455

K. Lee

{"title":"Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer","authors":"K. Lee","doi":"10.15324/KJCLS.2017.49.4.455","DOIUrl":"https://doi.org/10.15324/KJCLS.2017.49.4.455","url":null,"abstract":"MicroRNAs (miRNAs/miRs) are a group of small noncoding RNAs that modulate gene expression. Many studies, demonstrating altered expressions of specific miRNAs in diverse types of human neoplasia, suggested that they may play a key role in tumorigenesis. Recently, miRNA genes were found to be abnormally expressed in several types of cancer, including endometrial cancer. However, miR-23b and miR-203 expression in endometrial cancer has yet to be studied in Korea. As such, the purpose of this study was to analyze miR-23b and miR-203 expressions in endometrial cancer and to evaluate the relationship between miR-23b and miR-203 expressions. A retrospective study was carried out on the formalin-fixed, paraffin-embedded tissues of 42 endometrial cancer tissues using quantitative real-time PCR. In endometrial cancer tissues, miR-23b expression levels (2.70±4.45) were higher than miR-203 expression levels ( − 2.34±4.08). Endometrial cancer tissues showed an overexpression of miR-23b in 30 (71.4%) of the 42 endometrial cancer cases, whereas miR-203 was underexpressed in 29 (69.0%) of the 42 cases. There was a significant association between miR-23b and miR-203 expressions in endometrial cancer tissues (p=0.0005). These findings suggest that miR-23b and miR-203 expressions may be involved in endometrial carcinogenesis. More studies are needed to further define the relationship between miR-23b and miR-203 expressions and tissue-specific protein expression.","PeriodicalId":10080,"journal":{"name":"临床检验杂志","volume":"51 1","pages":"455-459"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88294901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Perception and Satisfaction Survey of Human Anatomy Dissection Course Taught by Health Science Departments: Biomedical Laboratory Science, Physical Therapy, and Occupational Therapy 卫生科学系生物医学检验、物理治疗、职业治疗人体解剖课教学感受及满意度调查

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.489

Se-Hoon Jung, Seung-Ju Ahn

引用次数: 0

The Development of Diagnosis for Atopic Dermatitis by Evaluating the Expression of Skin Barrier Proteins Using a Non-Invasive Method 应用无创方法评估皮肤屏障蛋白表达诊断特应性皮炎的进展

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.395

I. Kim, Ji-Sook Lee

{"title":"The Development of Diagnosis for Atopic Dermatitis by Evaluating the Expression of Skin Barrier Proteins Using a Non-Invasive Method","authors":"I. Kim, Ji-Sook Lee","doi":"10.15324/KJCLS.2017.49.4.395","DOIUrl":"https://doi.org/10.15324/KJCLS.2017.49.4.395","url":null,"abstract":"Atopic dermatitis is a chronic inflammatory skin disease caused by a variety of genetic and environmental factors, dysregulation of immunological response, as well as dysfunction of the skin barrier proteins. The purpose of this study is to develop an ELISA kit suitable for evaluating the expression of skin barrier proteins. Proteins were obtained from the skin via AriNo and D-Squame patches. The efficiency of protein collection from the skin, using the Arino patch, was shown to be more effective than using D-Squame; while the efficiency of lysis using 0.1% Triton-X100 was higher than that of other lysis solutions, including 0.1 M Tris-HCL, 0.1% Tween-20, and 5 mM KOH. Recombinant skin barrier proteins, such as filaggrin and involucrin, were produced by molecular biological methods. Monoclonal antibodies against filaggrin and involucrin were produced by immunization of mice, fusion of spleen cells and myeloma cells, as well as a selection of antibody-producing hybridoma cells. The filaggrin expression in the skin of subjects suffering from atopic dermatitis was lower than that in normal mice. Involucrin expression was not altered between normal individuals and subjects with atopic dermatitis. These findings contribute to an elucidation of the importance of the skin barrier protein expression in atopic dermatitis and the development of a diagnostic kit for atopic dermatitis.","PeriodicalId":10080,"journal":{"name":"临床检验杂志","volume":"28 1","pages":"395-400"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86208212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Relationship between the Lipid Accumulation Product Index and Alanine Aminotransferase in Korean Adult Men 韩国成年男性脂质积累产物指数与丙氨酸转氨酶的关系

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.374

K. Shin

{"title":"Relationship between the Lipid Accumulation Product Index and Alanine Aminotransferase in Korean Adult Men","authors":"K. Shin","doi":"10.15324/KJCLS.2017.49.4.374","DOIUrl":"https://doi.org/10.15324/KJCLS.2017.49.4.374","url":null,"abstract":"The LAP (lipid accumulation product) index is an indicator of excessive fat accumulation in combination with the fasting triglyceride concentration and waist circumference. This study examined the relationship between the LAP index and ALT (alanine aminotransferase) level and the effect of the LAP index on the ALT level. Cross-sectional studies were conducted on healthy adult males without a history of liver disease. From January 2015 to June 2017, 13,854 adults between 20 and 70 years of age, who underwent health screening at a general hospital in Gyeonggi-do, were enrolled in this study. The LAP index was calculated as [waist circumference (cm) − 65]×[triglyceride (mmol/L)] for males. Serum ALT abnormalities were set at 40 IU/L or more in males. All subjects underwent measurements of the anthropometric indicators and physiological examinations. A significant increase in ALT with increasing LAP quintiles was observed (p ＜ 0.001). The LAP index correlated with ALT (r=0.238, p ＜ 0.001). Insulin (r=0.449, p ＜ 0.001) and HDL-cholesterol (r= − 0.369, p ＜ 0.001) were strongly correlated with LAP. In addition, the LAP index is a factor affecting the ALT level (p ＜ 0.001). As the LAP index increased, the risk of an elevated ALT was higher (p ＜ 0.001). In conclusion, the LAP index was a predictor of an ALT elevation in Korean men.","PeriodicalId":10080,"journal":{"name":"临床检验杂志","volume":"12 1","pages":"374-381"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87649472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Contamination of Methicillin-Resistant Staphylococcus (MRS) in a High School Environment 某高中环境耐甲氧西林葡萄球菌(MRS)污染情况研究

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.420

S. Hong, Y. Baek

引用次数: 2

Epidemiologic Study of Zoophilic Dermatophytoses between 2010 and 2016 2010 - 2016年嗜兽性皮肤真菌病流行病学研究

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.439

S. Kim

引用次数: 1

Harmonization of Hemolysis Index in Clinical Chemistry Laboratory and Its Application as a Result Verification Tool 临床化学实验室溶血指标的统一及其作为结果验证工具的应用

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.350

Sang-Shin Pyo, H. S. Nam, Y. Cha, Seung-Koo Lee, H. Lee

{"title":"Harmonization of Hemolysis Index in Clinical Chemistry Laboratory and Its Application as a Result Verification Tool","authors":"Sang-Shin Pyo, H. S. Nam, Y. Cha, Seung-Koo Lee, H. Lee","doi":"10.15324/KJCLS.2017.49.4.350","DOIUrl":"https://doi.org/10.15324/KJCLS.2017.49.4.350","url":null,"abstract":"용혈 지수(hemolysis index)는 생화학 장비에서의 용혈을 위한 반정량 지표이다. 용혈 지수의 특성이 상업 플랫폼마다 다르기 때문에 용혈 지수의 표준화 또는 일치화는 현재 되어 있지 않다. 진단검사의학에서 일치화(harmonization)는 측정 절차와 상관없이 동일한 결과와 동일한 해석을 할 수 있는 능력을 말한다. 임상 환자의 혈액 중에서 무작위로 추출한 40개의 검체를 인위적으로 체외 용혈(in vitro hemolysis)시킨다. 혈액 자동화 장비인 XE-2100 (Sysmex, Japan)에서 측정된 혈청 헤모글로빈을 기준으로 검체의 혈청을 0∼300 mg/dL 사이에서 20mg/dL 차이로 각각을 희석시킨다. 희석된 혈청 헤모글로빈을 생화학 자동화 장비 Hitachi-7600 (Hitachi, Japan)으로 측정하여 용혈 지수와 혈청 헤모글로빈을 평가하였다. 용혈 지수와 혈청 헤모글로빈은 선형성을 보였으며 용혈 지수 1은 대략 혈청헤모글로빈 20 mg/dL 정도였다. 용혈 거절 기준을 결정하기 위해 용혈 지수를 세 그룹으로 나눈다: 용혈 지수 0∼1, 용혈지수4∼6, 용혈 지수 9∼15. 또 다른 임상 검체 40개의 검체를 Hitachi-7600 (Hitachi, Japan)로 각각의 임상 검사 종목을 측정한 후, 각각의 검체를 주사기의 피스톤을 앞·뒤로 움직여 인위적인 체외 용혈을 시키고 Hitachi-7600 (Hitachi, Japan)로 측정하였다. 세 그룹 사이의 임상 검사 종목의 용혈로 인한 백분율 차이(percentage difference)를 ANOVA 또는 Kruskal-Wallis test 분석하였다. 사후 검정에서 용혈 지수 0∼1인 그룹과 용혈지수 5∼6인 그룹 사이에 유의한 차이가 나는 종목들은 Glucose, creatinine, total protein, AST, direct bilirubin, uric acid, phosphorus, triglyceride, LDH, CPK, magnesium, potassium이였다. 많은 임상 검사 종목들이 용혈지수 4∼6인 그룹과 참조 그룹인 용혈지수 0∼1인 그룹 간에 통계적으로 유의미한 차이를 보이기 때문에 용혈 기준은 대략 용혈지수 5 (혈청 헤모글로빈 100 mg/dL)가 적합하다 판단된다.","PeriodicalId":10080,"journal":{"name":"临床检验杂志","volume":"33 1","pages":"350-358"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76891751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Nontuberculous Mycobacteria (NTM) from Air Conditioner Dust 空调粉尘中非结核分枝杆菌(NTM)的分离

临床检验杂志 Pub Date : 2017-12-31 DOI: 10.15324/KJCLS.2017.49.4.435

Seung-Gu Choi, Myeongjin Choi

{"title":"Isolation of Nontuberculous Mycobacteria (NTM) from Air Conditioner Dust","authors":"Seung-Gu Choi, Myeongjin Choi","doi":"10.15324/KJCLS.2017.49.4.435","DOIUrl":"https://doi.org/10.15324/KJCLS.2017.49.4.435","url":null,"abstract":"Nontuberculous mycobacteria (NTM) contains M. avium–intracellular complex (MAC), M. fortuitum, M. chelonae, M. abscessus, M. kansasii, etc., which causes infections in swine, poultry, and other animals. These bacteria are opportunistic pathogens that can contaminate people, and in doing so, cause various social economic problems. This study proved that NTM are also present in air conditioners of multiuse facilities in frequently visited populated areas. The results of this study are meaningful because they showed that pathogenic microbes can live in the surroundings and cause diseases to people with impaired immunity. This study used the AFB stain, L-J medium culture, and PCR method for the detection of MTB and NTM in air conditioner dust. MTB was not detected in any of the collected samples, while NTM was detected in 2 out of 40 samples (5%). Most people living in the modern environment cannot avoid being exposed to air conditioners so special attention is needed for sanitary inspections and the management of air conditioners. Moreover, it is important to study NTM through various individuals, accumulate the related data and establish the methods and standards of hygiene management system to reduce NTM infections in public areas. Moreover, it would be necessary to identify the precise species of NTM through DNA sequencing and evaluate the effects of NTM on the pulmonary tuberculosis in immunodeficiency patients.","PeriodicalId":10080,"journal":{"name":"临床检验杂志","volume":"14 1","pages":"435-438"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73221453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4