Journal of Natural Pesticide Research最新文献

{"title":"Biological control of Alternaria alternata MW970059, the causal agent of tomato rot, by Aspergillus nidulans MW732187 isolated from green household waste","authors":"Fatima Zahrae Moussaid ,&nbsp;Rachid Lahlali ,&nbsp;Said Ezrari ,&nbsp;Azeddin El Barnossi ,&nbsp;Abdelilah Iraqi Housseini","doi":"10.1016/j.napere.2025.100134","DOIUrl":"10.1016/j.napere.2025.100134","url":null,"abstract":"<div><div>The development of fungal resistance to fungicides is a significant challenge in agricultural disease management, and the research axis is in current scientific discourse. The objective of the current study aims to search for new biological agents to control <em>Alternaria alternata</em> (<em>A. alternata</em>) causal agent of tomato rot. 73 fungal isolates where tested in the <em>in vitro</em> screening. <em>Aspergillus</em> sp. isolated from decayed tangerine peel microflora, showed the performing antagonistic strain against <em>A. alternata</em>. <em>Aspergillus</em> sp. exhibited significant inhibition of <em>A. alternata</em>; the growth fungal inhibition (%FI) rate was 66.66 ± 0.25 % causing various morphological mycelia changes, swellings, deformations and vacuolization. <em>A. alternata</em> and the antagonist <em>Aspergillus</em> sp. were identified based on morphology trails and molecular tool as <em>A. alternata</em> and <em>Aspergillus nidulans</em>, respectively. Cell-free supernatant (CFS) showed that the metabolites produced by <em>A. nidulans</em> are involved in the control of <em>A. alternata</em>. The half maximal effective concentration (EC₅₀) value of <em>A. alternata</em> was estimated at 1 % (v/v), the inhibition rate was 50.97 ± 0.85 %. Fourier Transform Infra-Red (FT-IR Spectrometry) analysis of organic (ethanol, methanol) and aqueous extracts of CFS revealed distinct characteristic peak values corresponding to various suggested functional groups in the extracts such as phenolic, lipids, and possibly proteins/peptides compounds. <em>In vivo</em> application of <em>A. nidulans</em> strain to tomato fruits significantly reduced postharvest decay caused by <em>A. alternata</em> by 41.34 % after the 10^th day of inoculation. Overall, the findings from this study suggest that <em>A. nidulans</em> strain may be a potential promising bio-agent to control the phytopathogen <em>A. alternata</em>.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current and future perspectives on biopesticides analysis in soil","authors":"Milton C. Soto-Barajas ,&nbsp;Denisse Archundia ,&nbsp;Oscar G.R. Martínez ,&nbsp;Esaú López ,&nbsp;Joseph Almazan ,&nbsp;Blanca Prado","doi":"10.1016/j.napere.2025.100120","DOIUrl":"10.1016/j.napere.2025.100120","url":null,"abstract":"<div><div>Biopesticides are composed of natural elements such as microorganisms and their metabolites. Currently, these substances have gained popularity and are used in sustainable production models including organic agriculture and integrated pest management, which are characterized by partially or completely avoiding inputs of synthetic origin. There is a general belief that biopesticides are less harmful than chemical pesticides, due to their natural origin, low persistence, and lower toxicity. Nevertheless, specific biological compounds utilized as biopesticides have been reported to induce unintended effects, including the emergence of resistance in targeted species, disruptions to soil microbiota, and interference with soil enzymatic activity. Although certain aspects of these phenomena have been studied, there are still numerous uncertainties surrounding their interaction with soil, their subsequent destiny, and their environmental impact. Moreover, considering the growing prevalence of substances that have the potential to be used as commercial biopesticides and the worldwide increase in their utilization, it is imperative to conduct additional analyses. This literature review explores the current research on biopesticides, specifically their effects on soil environments and examines current methods of analysis and approaches for their quantification in soil samples.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and toxicity of Indian plant extracts to the larvae of the malaria vector Anopheles stephensi Liston 1901 (Diptera: Culicidae)","authors":"Subramanian Arivoli ,&nbsp;Samuel Tennyson","doi":"10.1016/j.napere.2025.100113","DOIUrl":"10.1016/j.napere.2025.100113","url":null,"abstract":"<div><div>The threat posed by mosquitoes has long been a problem in tropical and subtropical areas of the world. Numerous initiatives have been launched to combat mosquitoes, and studies on plant-based products that inhibit mosquito vectors have been proposed as potential substitutes for the widely used synthetic chemical insecticides that are harmful to non-target creatures, human health, and the environment. In this study, the larvicidal efficacy of solvent (hexane, diethyl ether, dichloromethane, ethyl acetate, and methanol) leaf extracts of Indian plants from 44 different families, 83 genera, and 100 species was tested at 1000 mg/L concentration against the early third instar of <em>Anopheles stephensi</em>, the protozoan parasite that causes malaria. Asteraceae family reported highest larvicidal activity with 38.8 %, followed by Fabaceae (27.4 %) and Piperaceae (24.0 %). Thereafter, the extracts of each promising plant (solvent extracts of plants that exhibited 100 % mortality) were tested at lower concentrations of 100, 200, 300, 400 and 500 mg/L for 24 and 48 h. All solvent extracts of <em>Piper longum</em>, <em>Piper nigrum</em>, <em>Piper sarmentosum</em>, <em>Tagetes erecta</em> and <em>Tagetes minuta</em> showed 100 % larval mortality, hence were chosen as the promising plants for further study. LC₅₀ values &lt; 100 mg/L were reported by the diethyl ether, dichloromethane and methanol extracts of <em>Piper nigrum</em>; ethyl acetate extract of <em>Piper sarmentosum</em>; and by the diethyl ether and dichloromethane extracts of <em>Tagetes minuta</em>. For <em>Piper</em> extracts, the dominant phytocompounds identified by GC-MS analysis were limonene, asaricin, phytol, β-asarone, piperine, pipericine, stearic acid, myristic acid, oleic acid, and piperitone; for <em>Tagetes</em> extracts, the dominant phytocompound was piperitone. The larvicidal property of <em>Piper</em> extracts be due to the action of fatty acids and alkaloids present in them, and for <em>Tagetes</em> extracts, due to the presence of flavonoids, tannins and terpenoids. Further, the present study has documented the first scientific report on the larvicidal activity of <em>Piper sarmentosum</em> leaf extracts against <em>Anopheles stephensi</em>.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial control of forest insect pests over 60 years (1964–2024): Network analysis and bibliometric mapping","authors":"Deepak Kumar Mahanta ,&nbsp;Charishma Krishnappa ,&nbsp;Tanmaya Kumar Bhoi","doi":"10.1016/j.napere.2025.100132","DOIUrl":"10.1016/j.napere.2025.100132","url":null,"abstract":"<div><div>Microbial control of forest insects has emerged as a viable alternative to conventional chemical pesticides, providing sustainable strategies for managing forest pest populations. This bibliometric analysis examines the progression of research in this domain from 1964 to 2024, emphasizing the application of entomopathogenic fungi, bacteria, viruses, and nematodes as biological control agents. VOSviewer, Bibliometrix, and Biblioshiny from R were employed for statistical analysis of the articles retrieved from the Scopus database. The research indicates a substantial rise in articles concerning microbial control during the early 2000s, underscoring an increasing awareness of its potential in forest management. Prominent biocontrol agents, including <em>Beauveria bassiana</em>, <em>Metarhizium anisopliae</em>, and <em>Bacillus thuringiensis</em>, have been recognized for their efficacy in managing diverse forest pests, such as the gypsy moth (<em>Lymantria dispar</em>), bark beetles, and defoliators. The research highlights the increasing significance of microbial agents in minimizing pest outbreaks intensified by climate change, invading species, and the constraints of chemical pesticides. Notwithstanding the optimistic outlook, the study also delineates additional challenges, such as ecological hazards, variable efficacy across different climatic conditions, regulatory obstacles, and the absence of long-term field data. These problems underscore the necessity for more studies to enhance the use of microbial management in forest ecosystems, ensuring its efficacy and safety. This research offers a thorough examination of the present condition of microbial control in forest pest management, highlighting the necessity of creating dependable, environmentally sustainable alternatives to maintain forest health and biodiversity amid increasing environmental challenges.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive principle from Pentanema indicum against Aedes aegypti and Culex quinquefasciatus","authors":"Prince Paul,&nbsp;Kabilan Muralidharan,&nbsp;Duraipandiyan Veeramuthu","doi":"10.1016/j.napere.2025.100125","DOIUrl":"10.1016/j.napere.2025.100125","url":null,"abstract":"<div><div>Mosquitoes wreak havoc in human lives as they are responsible for transmitting several diseases that are both life-threatening and fatal. In spite of all efforts to control the spread of mosquitoes and reducing their population, they are spreading widely and becoming more virulent. Some of the mosquito species have gained resistance to commercially available synthetic and chemical insecticides. Developing alternative natural insecticides is one of the most effective ways to combat vector mosquitoes. Secondary metabolites of plant origin are extensively used and come to the aid of humans. In the present study, the bioefficacy of <em>Pentanema indicum</em> is tested against immature stages of mosquitoes. Four extracts of <em>P. indicum</em> were employed for the larvicidal bioassay. Ethyl acetate extract possessed highest bioefficacy with LC₅₀ values of 24.253 ppm and 26.020 ppm followed by hexane extract with 216.853 ppm and 66.030 ppm, chloroform with 359.611 ppm and 114.793 ppm, methanol extract demonstrated 161.709 ppm and 874.258 ppm against <em>Ae. aegypti</em> and <em>Cx. quinquefasciatus</em> respectively. The chromatogram (GC-MS) of the active ethyl acetate extract contains 23 phyto principles and the major compound is 1,2,3-propanetriol, 1-acetate. This study reveals that <em>P. indicum</em> possesses great larvicidal potency against mosquitoes and it could be a promising plant to control the mosquito population.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100125"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive secondary metabolites from Curvularia spp.: Natural alternatives for pest management in agriculture","authors":"Victor Pena Ribeiro ,&nbsp;Joanna Bajsa-Hirschel ,&nbsp;Prabin Tamang ,&nbsp;Marcuslene D. Harries ,&nbsp;Kumudini M. Meepagala","doi":"10.1016/j.napere.2025.100117","DOIUrl":"10.1016/j.napere.2025.100117","url":null,"abstract":"<div><div>The widespread use of pesticides in agriculture has raised concerns due to their environmental impact, development of resistant pest strains, and potential health risks. As a result, interest in safer alternative compounds for pest management is increasing. Fungal secondary metabolites, renowned for their structural diversity and biological activities, have emerged as promising alternatives to synthetic pesticides. The utilization of these natural compounds offers a sustainable approach to managing agricultural pests. This study investigates the chemical composition and biological activities of secondary metabolites isolated from a <em>Curvularia spp.</em>, focusing on their phytotoxic and antifungal properties. Seven compounds—tyrosol, phenethyl alcohol, 4-hydroxybenzaldehyde, 3-(4-hydroxyphenyl)propionic acid, 4-(3-hydroxypropyl)phenol, tyramine, and <em>N</em>-(4-hydroxyphenethyl)acetamide—were isolated from the ethyl acetate extract of the fungus, marking the first report of these metabolites in Curvularia species. The ethyl acetate extract demonstrated significant phytotoxicity against <em>Agrostis stolonifera</em>, a monocot, with tyrosol and 4-hydroxybenzaldehyde identified as the active compounds. Additionally, phenethyl alcohol and N-(4-hydroxyphenethyl)acetamide exhibited antifungal activity against <em>Colletotrichum fragariae</em>, the causative agent of strawberry anthracnose. These findings support the potential of <em>Curvularia spp</em>. as a source of bioactive compounds with applications in agriculture and plant disease management for sustainable crop protection strategies.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness, characterization and optimization of botanical extracts: An ecofriendly approach for management of thrips infestation on tea in Bangladesh","authors":"Md. Amjad Patwary ,&nbsp;M.A.K. Azad ,&nbsp;Iftekhar Ahmad ,&nbsp;Md. Kashem Ali ,&nbsp;Jahid Hasan Shourove ,&nbsp;Jenat Yeasmin ,&nbsp;G.M. Rabiul Islam","doi":"10.1016/j.napere.2025.100118","DOIUrl":"10.1016/j.napere.2025.100118","url":null,"abstract":"<div><div>In the realm of agricultural pest management, the exploration of eco-friendly solutions remains paramount. This study delves into the efficacy of botanical extracts derived from medicinal plants in combating thrips infestation on tea plants (<em>Camellia sinensis</em> L.) in Bangladesh. Through meticulous experimentation, this study found <em>Sapium indicum</em> (Willd.) to be the most effective plant extract, followed by <em>Ipomoea carnea</em> (Jacq.) and <em>Lantana camara</em> where the LC₅₀ values were recorded as 431.28 ppm, 590.83 ppm, and 1329.03 ppm, respectively after 24 h of exposure. Additionally, volatile compounds of <em>S. indicum</em> showed the highest thrips control efficacy, approximately on average 75 % mortality, while having the lowest CV. Notably, under optimized conditions of 24.4 h stirring time, 56.8 °C, and 54.8 % ethanol concentration, <em>S. indicum</em> extract achieved 89.55 % efficacy whereas leaving minimal soil residue (43.4 mg/kg), demonstrating both its potency and low environmental persistence. Gas chromatography-mass spectrometry (GC-MS) was used to conduct chemotaxonomic analyses, revealing isobutyl tiglate as a particularly potent component, alongside 2-methyl-2propyl-1,3-propanediol, showcasing promising efficacy against tea thrips. These insights underscore the potential of botanical extracts as sustainable and eco-friendly alternatives for thrips management in agricultural ecosystems, while advocating for the integration of chemotaxonomic approaches in shaping effective pest control strategies.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image-based blight disease detection in crops using ensemble deep neural networks for agricultural applications","authors":"Md Mohinur Rahaman ,&nbsp;Saiyed Umer ,&nbsp;Md Azharuddin ,&nbsp;Asmaul Hassan","doi":"10.1016/j.napere.2025.100130","DOIUrl":"10.1016/j.napere.2025.100130","url":null,"abstract":"<div><div>Blight disease poses a significant threat to agricultural output that results in large crop losses worldwide. Plant diseases must be promptly identified and managed to maintain crop health and maximise yields. This research presents a novel ensemble-based deep-learning model for plant blight disease detection, especially for agricultural applications. The suggested model uses convolutional neural networks (CNNs) for image recognition to accurately and automatically detect blight-affected areas in plant leaf images. An extensive dataset of plant leaf images was gathered to train and evaluate the model, including samples from both healthy and diseased plants. This ensemble-based deep learning model outperformed conventional deep learning and machine learning models in extracting characteristics that differentiated between plants affected by blight and those that weren’t. The proposed model (ResNet11) is a dependable and effective tool for on-the-spot disease detection in the field of potato, tomato and pepper, as demonstrated by experimental results that illustrate an accuracy of over 99 % for potato and pepper crops as a 3-class and 2-class problem respectively. Moreover, we get an accuracy of over 87 % for tomato plants as a 10-class problem.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100130"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, characterization and antifungal activity of Bacillus antagonistic bacteria from decomposing solid organic waste against Alternaria alternata and Fusarium oxysporum","authors":"Fatima Zahrae Moussaid ,&nbsp;Rachid Lahlali ,&nbsp;Said Ezrari ,&nbsp;Nabil Radouane ,&nbsp;Abdelilah Iraqi Housseini","doi":"10.1016/j.napere.2025.100124","DOIUrl":"10.1016/j.napere.2025.100124","url":null,"abstract":"<div><div>Plant diseases and pathogens affect agricultural crop production and sustainable development worldwide. Although chemical tools for controlling the diseases in crops are available, many of them exhibit serious impacts, especially on human and animal health and environmental pollution. Biological control could be an alternative approach towards managing disease in crops that promotes eco-friendly agricultural practices for sustainable environments. <em>Alternaria</em> and <em>Fusarium</em> diseases are a major source of loss to farmers all over the world; they affect many vital vegetable and food crops. This study sought to evaluate the <em>in vitro</em> antifungal properties of bacterial strains isolated from solid organic waste, green household waste and poultry droppings against <em>Alternaria alternata</em> and <em>Fusarium oxysporum</em> phytopathogenic fungi. The antagonist <em>Bacillus</em> bacteria were screened based on <em>in vitro</em> direct confrontation with <em>A. alternata</em> and <em>F. oxysporum</em>; among 110 isolates, 9 strains were selected for their antifungal potential against <em>F. oxysporum</em> and <em>A. alternata</em>. Using the 16S rRNA gene, the selected <em>Bacillus</em> bacteria were identified at the species through molecular identification as <em>B. siamensis, B. amyloliquefaciens</em> and <em>B. subtilis</em>. All antagonist strains were characterized for their possible mechanisms of biocontrol traits involved in these antifungal activities. Results showed that all selected <em>Bacillus</em> bacteria were found to be pectinase and amylase producers. However, only eight bacteria were found to produce proteases. Bioassays with the cell-free supernatants (CFSs) produced from <em>B. amyloliquefaciens, B. siamensis</em> and <em>B. subtilis</em> showed a significantly inhibited growth of <em>F. oxysporum</em> and <em>A. alternata</em>. Identifying effective biocontrol agents is challenging, especially in resource-limited settings, and their efficacy varies due to environmental and strain-specific factors. The findings indicate that antifungal compounds from <em>B. siamensis, B. amyloliquefaciens</em> and <em>B. subtilis</em> could be an alternative approaches as biocontrol agents to fungal diseases, improving crop yields and sustainable agriculture.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100124"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS based phytoconstituents profiling, toxicity assessment, and potential of Genipa americana L. leaf extracts as natural insecticides against striped mealybugs Ferrisia sp.","authors":"Mike Vázquez-Torres ,&nbsp;Nilka Rivera-Portalatín ,&nbsp;Irma Cabrera-Asencio","doi":"10.1016/j.napere.2025.100123","DOIUrl":"10.1016/j.napere.2025.100123","url":null,"abstract":"<div><div>Plant-based botanical insecticides are emerging as a promising solution for crop protection while minimizing concerns related to environmental pollution and insect resistance due to their natural origin. Leaf extracts from the folkloric medicinal plant <em>Genipa americana</em> L. (Rubiaceae) were obtained through micro-Soxhlet extraction and subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis to identify their phytochemical constituents. GC-MS profiling revealed that these extracts were mainly composed of carboxylic acids, esters, steroids, phenols, triterpenes, and other bioactive secondary metabolites. Phytocompounds with different biological activities such as di-isobutyl phthalate, hexadecanoic acid, linoleic acid, squalene, α-tocopherol, and stigmasterol were identified. Toxicity assessment through brine shrimp lethality tests proved the potential pesticidal activity of two of these extracts. The chloroform extract (GAC) was the most toxic against <em>Artemia salina</em> with an LC₅₀ value of 92.85 μg/mL followed by the dichloromethane extract (GAD), which also resulted toxic but less than the positive control, with an LC₅₀ of 485.46 μg/mL. Contact toxicity bioassays with <em>Genipa</em> leaf extracts against the striped mealybugs <em>Ferrisia</em> sp. (Hemiptera: Pseudococcidae) demonstrated that the GAC extract has the maximum insecticidal response, with an LC₅₀ of 7.89 mg/mL, followed by GAD extract, with an LC₅₀ of 16.42 mg/mL. This is the first study in which <em>Genipa americana</em> leaf extracts are assessed for their insecticidal activity against striped mealybug insects spotlighting the potential of both GAC and GAD leaf extracts as natural insecticides against this pest of negative economic impact on agriculture.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100123"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}