Journal of Natural Pesticide Research最新文献

{"title":"Actinobacteria mediated synthesis of silver nanoparticles using Streptomyces diastaticus and their biological efficacy against human vector mosquitoes and agricultural insect pests","authors":"Manickam Kalpana ,&nbsp;Krishnan Raguvaran ,&nbsp;Thulasiraman Manimegalai ,&nbsp;Suresh Kalaivani ,&nbsp;Palanisamy Devapriya ,&nbsp;Rajan Maheswaran","doi":"10.1016/j.napere.2024.100091","DOIUrl":"10.1016/j.napere.2024.100091","url":null,"abstract":"<div><p>Mosquitoes and agricultural insects were harmful to both public health and crops. Prolonged use of synthetic chemical pesticides for insect pest control was associated with resistance development and negative human and ecological effects. This study investigates the larvicidal activity of Ag-NPs produced using <em>Streptomyces diastaticus</em> against <em>Culex quinquefasciatus</em>, <em>Anopheles stephensi</em>, <em>Aedes aegypti</em>, <em>Spodoptera litura</em>, and <em>Plutella xylostella</em>. UV-Vis spectroscopy, XRD, SEM-EDX, FT-IR, TEM, DLS, and Zeta potential analysis were used to characterize the biosynthesized Ag-NPs. The results showed that biosynthesized Ag-NPs were more toxic to, <em>A. stephensi</em>, <em>C. quinquefasciatus</em>, and <em>A. aegypti</em> with LC₅₀ values of 15.70, 11.10, and 17.53 µg/mL, respectively. Biosynthesized Ag-NPs exhibited the highest antifeedant effects against <em>S. litura</em> (88.73 %) and <em>P. xylostella</em> (92.36 %) at 50 µg/mL concentration. The mortality bioassay revealed considerable larvicidal activity against <em>S. litura</em> and <em>P. xylostella</em> larvae at greater doses of 50 µg/mL, 80.67 and 84.05 %, respectively. Morphogenetic variations were observed on treated larvae of <em>C. quinquefasciatus, A. aegypti, and A. stephensi, S. litura,</em> and <em>P. xylostella</em> with Ag-NPs. In the present investigation the impact of Ag-NPs on the antioxidant enzymes (SOD, CAT, and GPx), the activity of each enzyme were dramatically increased with higher concentrations resulting in significant differences. The study also found that Ag-NPs inhibited the detoxifying enzyme (GST and AChE) activity of target organisms. The histological structure of the treated and untreated larvae revealed structural changes between the midgut epithelial cells. As a consequence of these findings, the <em>S. diastaticus</em>-mediated Ag-NPs serve as a possible, eco-friendly biopesticide and alternative to synthetic chemicals for the control of human vector mosquitoes and destructive arthropod insect pests.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000268/pdfft?md5=20964b29cb45e07565d3f03d61674643&pid=1-s2.0-S2773078624000268-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141844372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops","authors":"M.F. Amir Hafiz ,&nbsp;Farah B. Ahmad ,&nbsp;M.H. Maziati Akmal","doi":"10.1016/j.napere.2024.100092","DOIUrl":"10.1016/j.napere.2024.100092","url":null,"abstract":"<div><p>The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as the sensing mechanism of the biosensor. In this study, microbial esterase from <em>Rhizopus oryzae</em> (RoE) was immobilized into modified screen-printed carbon electrode (SPCE) for the fabrication of novel RoE/chitosan/nanomaterial-COOH/SPCE biosensor for the detection of methyl parathion, an OP pesticide. The first phase of this study aims to analyze biochemical characteristics of 1-naphthyl acetate (1-NA) hydrolysis by RoE and enzymatic inhibition of RoE by OP pesticide. RoE kinetic showed the best fit with Langmuir model with the highest linear regression (<em>R</em>² = 0.996). The inhibition of RoE by OP was shown to be linearly proportional to the enzyme activity (<em>R</em>² = 0.827). The second phase of this study aims to analyze electrochemical performance of RoE-immobilized biosensor. Various carbon-based sensing support nanomaterials (graphene nanoplatelets (GNP), graphite and multi-walled carbon nanotube (MWCNT)) was functionalised by carboxyl (-COOH) group and screened, where all nanomaterials showed effective sensitivity towards detecting 0.01 ng/L methyl parathion pesticide. GNP-based biosensor exhibited the highest degree of inhibition (9.94 %) and better reproducibility after the first cycle. RoE/chitosan/GNP-COOH/SPCE biosensor is promising to be developed as cost-effective and highly sensitive biosensor for OP pesticide detection.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277307862400027X/pdfft?md5=7c76a734a63fefa2bdce3b6b24901a8b&pid=1-s2.0-S277307862400027X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovicidal, larvicidal and pupicidal activity of the ethyl acetate extracts from soil actinomycetes against tobacco caterpillar, Spodoptera litura (Lepidoptera: Noctuidae)","authors":"M. Kabilan ,&nbsp;Prince Paul ,&nbsp;V. Duraipandiyan ,&nbsp;M. Muthupandi","doi":"10.1016/j.napere.2024.100090","DOIUrl":"10.1016/j.napere.2024.100090","url":null,"abstract":"<div><p>Actinomycetes are known to produce wide-spectrum of secondary metabolites which has a wide variety of biological activities including insecticidal property. <em>Spodoptera litura</em> is a serious pest that damage various commercial cash crops which directly affects the agricultural productivity. The present study is to isolate soil actinomycetes from the terrestrial soil samples collected from the eastern ghats and screened them with various biological screening methods. There were around 100 actinomycetes isolate were obtained using the serial dilution method. It was subjected to antimicrobial screening using streak method (data not shown) and identified active isolates. The most active seven isolates were identified and their secondary metabolites were extracted using ethyl acetate as solvent. All the seven isolates of ethlyacetae extracts were performed the ovicidal, larvicidal and pupicidal bioassays against agricultural insect pest <em>Spodoptera litura</em>. Based on the calculated LC50 values, the most effective crude extract was found to be <em>Streptomyces</em> Sp<strong>.</strong> ERI-TVM-055, ethyl acetate extract. It has exhibited 100 % ovicidal activity at lowest LC50 values of 2.3330 ppm and larvicidal activity was exhibited the 100 % at 25.423 ppm of LC50. It also, exhibited the 100 % pupicidal activity at LC50 of 6.273 ppm<em>.</em> Similarly, <em>Streptomyces</em> sp. ERI-TVM-057, ethyl acetate extract exhibited ovicidal activity against <em>S. litura</em> eggs at LC50 of 11.025 ppm. Over all tested all actinomycetes isolate, the most significant isolate is <em>Streptomyces</em> Sp<strong>.</strong> ERI-TVM-055. The most active extract subjected to GC-MS analysis and the major bio-active molecules were identified. Several compounds present in the crude extract by the study of GC-MS spectrum. The major compounds were identified as Benzene acetic acid (30.19 %) and Acetamide, N-(2- Phenylethyl) (68.37 %) for the insecticidal activity of the active actinomycetes. The present study concluded that <em>Streptomyces</em> Sp<strong>.</strong> ERI-TVM-055 ethyl acetate extract can be used as microbial pesticides as effective one against agricultural pest <em>S. litura</em> and it leads to identify the active molecules and safe the environments.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000256/pdfft?md5=dc2cb74fc9903bb2304c15a328ab093f&pid=1-s2.0-S2773078624000256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PyPI: An internet-enabled learning tool to boost learner motivation for crop protection","authors":"Yoshiyasu Takefuji","doi":"10.1016/j.napere.2024.100089","DOIUrl":"10.1016/j.napere.2024.100089","url":null,"abstract":"<div><p>Instructors are always interested in methods to activate learner incentives and motivation to increase learning effectiveness. This paper introduces the Python Package Index (PyPI) as a powerful tool to maximize learner incentives on software and presents an example of its application in agriculture and science. The more useful the PyPI application is, the more it will be downloaded worldwide, providing an external review for the learner, and strengthening their incentive. However, many existing tutorials on PyPI, including the official site, are not updated on the twine library for uploading files to the PyPI site. This paper presents an updated tutorial on using PyPI for counting disaggregated objects such as bugs and pests, and for software reproducibility validation via Code Ocean. Additionally, generative AI is introduced as an indispensable assistant for tasks such as understanding technical terms and providing solutions for encountered problems.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000244/pdfft?md5=d8bf16af3f845d5a10d0045d141391c1&pid=1-s2.0-S2773078624000244-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141692751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring chemical composition and insecticidal activities of Alpinia calcarata rhizome essential oil against three major storage insects","authors":"Pathikalayil R. Anuranj,&nbsp;Naduvilthara U. Visakh,&nbsp;Berin Pathrose,&nbsp;Shiela Betsy George","doi":"10.1016/j.napere.2024.100088","DOIUrl":"10.1016/j.napere.2024.100088","url":null,"abstract":"<div><p><em>Alpinia calcarata</em> (Roscoe), generally recognized as snap ginger or lesser galangal of the Zingiberaceae family, undergoes widespread cultivation in tropical nations. It is characterized by its noteworthy pharmacological attributes, such as anti-inflammatory, analgesic, antinociceptive, reproductive, anti-cancer, and gastro-protective effects. Many storage pests now resist traditional chemical pesticides. Transitioning to organic alternatives is a preferable and sustainable solution. This research investigates the insecticidal activity of essential oil from <em>A. calcarata</em> rhizomes, obtained via hydro-distillation. Chemical analysis was conducted, and the study assessed its toxicity and repellent effects on <em>Tribolium castaneum</em> (Herbst), <em>Lasioderma serricorne</em> (F.), and <em>Callosobruchus chinensis</em> (L)<em>.</em> A yield of 0.44 ± 0.02 % essential oil was obtained from dry rhizomes. Gas chromatography-mass spectrometry (GC-MS) characterization revealed the major chemical constituents as 1,8-cineole (25.71 %), ethyl iso-allocholate (16.01 %), and fenchyl acetate (15.15 %). The findings from the investigation into the pesticidal characteristics of essential oils derived from the rhizome of <em>A. calcarata</em> unveiled significant contact (LC₅₀ at 24 h was 10.9 mg/cm² for <em>T. castaneum</em>, 1.0 mg/cm² for <em>L. serricorne</em> and 0.5 mg/cm² for <em>C. chinensis</em>) and fumigant toxicity (LC₅₀ at 24 h was 4.05 mg/L air for <em>T. castaneum</em>, 1.75 mg/L air for <em>L. serricorne,</em> and 1.03 mg/L air for <em>C. chinensis</em>). The repellent efficacy was evaluated through an area preference test, revealing robust repellent activity at various concentrations. Undoubtedly, the essential oil extracted from the rhizome of <em>A. calcarata</em> is valuable and has the potential to be a safe, organic pesticide for controlling storage pests.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000232/pdfft?md5=a783047aac7fe8f2713818ac95ba3d1f&pid=1-s2.0-S2773078624000232-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal potential of ether extract, and its derivatives fatty acids, and fatty acid methyl esters from Tecoma stans seeds: Bioprospecting for an effective insecticide","authors":"Lucas S. Azevedo ,&nbsp;Thaís P.R. Gonçalves ,&nbsp;Mairon C. Coimbra ,&nbsp;Ezequias P. de Siqueira ,&nbsp;Stênio N. Alves ,&nbsp;Luciana A. R. dos S. Lima","doi":"10.1016/j.napere.2024.100087","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100087","url":null,"abstract":"<div><p>In the work, the larvicidal potential of ether extract (EE), and its hydrolyzed products enriched with fatty acids (FA), and fatty acid methyl esters (FAME) obtained from <em>Tecoma stans</em> seeds was evaluated. EE, and FAME were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and three samples were evaluated in a preliminary screen for larvicidal activity against <em>Culex quinquefasciatus</em> at concentrations of 31.25, 62.5, 125, 250, 500, and 1000 μg/mL. The larvicidal efficacy of all samples was assessed at 24, 48, and 144 h post-treatment, detecting the movement of each larva. The data obtained were subjected to probit analysis to determine the lethal concentrations that killed 50 % and 90 % (LC₅₀ and LC₉₀) of the treated larvae. In EE, the phytosterol γ-sitostenone was characterized as the predominant component (30.40 %), while in FAME the presence of methyl palmitate (25.01 %), and methyl stearate (22.14 %) stood out. Notable larvicidal activity was detected after 24 h of treatment. EE exhibited moderate toxicity against <em>C. quinquefasciatus</em> (LC₅₀ = 193.34 μg/mL), while FAME was slightly toxic (LC₅₀ = 269.43 μg/mL), and FA, the most promising product, presented an LC₅₀ = 20.06 μg/mL. The results together demonstrate that extracts, and hydrolyzed products from <em>T. stans</em> seeds may be useful as eco-friendly, economical, and effective larvicidal agents. However, additional studies are needed to search for bioactive compounds of plant extracts, and their derivatives that exhibit larvicidal activity, and their modes of action.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000220/pdfft?md5=0239c6a255e19843fb4ebeabf1e8d7fe&pid=1-s2.0-S2773078624000220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals, UPLC-QTOF-MS/MS analysis, potential acaricidal activity and molecular modeling of Datura metel grown in North-Eastern India","authors":"Aditi Kundu ,&nbsp;Karivaradharajan Swarnalakshmi ,&nbsp;Sushmita Rajkhowa ,&nbsp;Anirban Barik ,&nbsp;S.N. Bhagyasree ,&nbsp;Tankeswar Nath ,&nbsp;Bidyut Kumar Sarmah ,&nbsp;Srikant Ghosh","doi":"10.1016/j.napere.2024.100086","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100086","url":null,"abstract":"<div><p>The present investigation emphasized exploitation of <em>Datura metel</em> L. for potential acaricidal activity. Extraction method suggested higher extraction efficiency with the use of 50 % methanol over aqueous medium. UPLC-QTOF-ESI-MS analysis of the extracts resulted tentative identification of eighteen tropane alkaloids such as tigloidin, hyoscyamine N-oxide, scopolamine N-oxide, hyocyamine, hydroxyapoatropine, tropinone, scopine, hyoscine, atropine, valtropine, ditigloyloxytropane, ditigloyloxytropanol, apohyoscine, tigloyloxytropane, norhyoscine, meteloidine, cuscohygrine and tropine in the aerial parts (EAP) and roots (ER). Further, nine phenolic acids such as gallic, protocatechuic, chlorogenic, vanillic, p-coumaric, ferulic, quinic, syringic, and sinapic acids along with fourteen flavonoids such as luteolin-7-glucoside, catechin-3-O-rhamnoside, kempferol-3,7-O-diglucoside, naringenin-6-β-D-glucopyranoside, quercetin 3-O-glucosyl-xyloside, apigenin-7-O-glucoside, flavonol-3-glucoside, luteolin, epicatechin, chrysoeriol, diadzein, genistein, hesperidin and hydroxycoumarin were also identified. Highest acaricidal activity was observed for ER showing LC₅₀ and LC₉₀ of 112.5 and 317.3 μg/mL after 48 h of exposure, respectively. Molecular modeling analysis revealed favourable interaction of tigloidine with UDP transferase of <em>T. urticae</em> through attractive charge, pi-alkyl, alkyl and conventional H bonds. Though, ER was found to be phytotoxic with the highest root growth inhibition in terms of IC₅₀ value of 131.2 and 184.9 μg/mL against <em>Chenopodium album</em> and <em>Triticum aestivum</em>, respectively. Being eco-benign natural products, <em>Datura</em> phytochemicals can serve as potential bio-acaricide, thereby, a smart delivery system could be developed comprising synergistic concoction of the alkaloid rich phytochemical fractions for efficient management of the notorious acarids.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000219/pdfft?md5=c9ca56be676733a1d772d86cfeba1af7&pid=1-s2.0-S2773078624000219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141434959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of essential oils from six Moroccan plants against the causal agent of Verticillium wilt of olives","authors":"Rachid Azenzem ,&nbsp;Tayeb Koussa ,&nbsp;Mohamed Najib Alfeddy","doi":"10.1016/j.napere.2024.100085","DOIUrl":"10.1016/j.napere.2024.100085","url":null,"abstract":"<div><p>In the realm of sustainable agriculture, biocontrol is gaining considerable attention for its focus on the development of safe, biodegradable, and environmentally friendly alternatives to traditional biocides. This study explored the potential use of essential oils as alternative biocontrol products for managing <em>Verticillium dahliae</em>, the causal agent of Verticillium wilt in olive trees. Essential oils (EOs) were obtained through hydrodistillation from six Moroccan aromatic and medicinal plants (<em>Ammodaucus leucotrichus</em>, <em>Mentha spicata</em>, <em>Origanum majorana</em>, <em>Rosmarinus officinalis</em>, <em>Salvia officinalis</em>, and <em>Thymus satureioides</em>). These EOs were then subjected to chemical analysis by gas chromatography-mass spectrometry (GC-MS) and evaluated in vitro for their antifungal properties against <em>V. dahliae</em>. The bioassays revealed that all tested EOs reduced mycelial growth of the studied strain, and tended to have dose-dependent effects. Compared with the various essential oils evaluated in this study, <em>A. leucotrichus</em> essential oils showed the most pronounced antifungal efficacy at low and moderate concentrations. These EOs stand out for their high content of two monoterpenes, perillia aldehyde (84.19 %) and limonene (14.15 %), which were not found in the other essential oils examined. Therefore, our results open up new possibilities for exploiting these plants and their chemical constituents to develop a natural biofungicide as a component of integrated management strategies for Verticillium wilt of olive and other crops.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000207/pdfft?md5=e608234ce3fb5cfd3c85a4f372129787&pid=1-s2.0-S2773078624000207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some aspects concerning citronella grass essential oil and the agroecological approach to protecting stored cowpea beans","authors":"Marcela de Souza Alves ,&nbsp;Elisabeth Alves Duarte Pereira de Medeiros ,&nbsp;Camila da Silva Barbosa Pereira ,&nbsp;Cristiane Martins Cardoso ,&nbsp;Emerson Guedes Pontes ,&nbsp;André Marques dos Santos ,&nbsp;Marco Andre Alves de Souza","doi":"10.1016/j.napere.2024.100084","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100084","url":null,"abstract":"<div><p>This study aimed to determine the impact of hydrodistillation duration on the chemical composition of citronella-grass essential oil. Furthermore, the oil's efficacy against cowpea weevil was evaluated by carrying out biological activity tests. The essential oil was obtained and chemically characterized. Subsequently, it was tested at different concentrations to evaluate its impact on the reproductive cycle and the total activity of the insects' biotransformation enzymes. Oxygenated monoterpene comprised the majority of the substances present. The mathematical model fitted to the extraction kinetics predicts that after 60 minutes of hydrodistillation, approximately 96% of essential oil, 94% of monoterpenes, and 50% of sesquiterpenes will be obtained with confidence. The fumigating effect of the citronella-grass essential oil resulted in increased mortality, reduced oviposition, and lower emergence of adults. The essential oil at sublethal concentration (0.09 mg cm^-3 for 6 hours) significantly reduced the total activity of biotransformation enzymes (acetylcholinesterase, α- and β-esterase, carboxylesterase) in female cowpea weevils.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100084"},"PeriodicalIF":0.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000190/pdfft?md5=94f2e0f4b0c29d471d1be1b5845f26f3&pid=1-s2.0-S2773078624000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141243612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-black pod disease activity of leaf extract of Carica papaya Linn","authors":"Tamekloe Woasiedem ,&nbsp;Ishmael Amoako-Attah ,&nbsp;Francis Ackah Armah ,&nbsp;Isaac Asiamah","doi":"10.1016/j.napere.2024.100082","DOIUrl":"10.1016/j.napere.2024.100082","url":null,"abstract":"<div><h3>Background</h3><p><em>Theobroma cacao (</em>Cocoa) is a major foreign exchange earner for Ghana. Over the recent decades, there has been a consistent decline in cocoa production in the country. A major contributing factor is the cocoa black pod disease caused by <em>Phytophthora</em> species. Synthetic fungicides have been used to control the pathogens over the years. However, recent calls for organic cocoa devoid of residues from synthetic fungicides, which are known to pose several health challenges as well as a negative impact on the environment, have gained attention. Thus, inviting investigations into more environmentally friendly and sustainable alternatives to synthetic fungicides.</p></div><div><h3>Objective</h3><p>This study explored the inhibitory potential of crude extract from <em>Carica papaya</em> against cocoa black pod disease caused by <em>Phytophthora</em> spp.</p></div><div><h3>Material and methods</h3><p>An <em>in-silico</em> study was done using known phytochemicals from <em>Carica papaya</em> as ligands against a <em>Phytophthora</em> effector protein. Crude extracts were obtained from the plant materials using 70 % ethanol as the extraction solvent. The crude Green Leaf Extract (GLE), Aging Induced Chlorophyll Deficient Leaf Extract (AICDLE), and Matured Black Seed Extract (SDE) were tested <em>in-vitro</em> against pre-cultured <em>P. palmivora</em> and <em>P. megakarya</em> using the poison food technique. GLE was modulated with Delco, a synthetic fungicide, at different ratios which were tested <em>in-vitro</em> and <em>in-vivo</em>.</p></div><div><h3>Results</h3><p>A total of 16 ligands showed binding affinity higher or equal to the standard ligand, alliin, suggesting <em>Carica papaya</em> as a repository of anti-phytophthora agents. Assessment of crude extracts at concentrations 5–20 mg/ml showed fungistatic activity against <em>P. palmivora</em> and <em>P. megakarya in-vitro</em>. Modulation of GLE with Delco produced some combinations that made GLE fungicidal <em>in-vitro</em> against <em>P. palmivora</em> and <em>P. megakarya</em> with fractional inhibition concentration indices between 0.51 and 0.65, interpreted as partially synergistic. The <em>in-vivo</em> assessment of combination D (Delco and GLE in a 0.3 mg:3 mg per ml ratio) performed as well as Delco alone at the recommended dose of 5 mg/ml. Thus, combination D reduces the requirement for copper-based fungicide by over 90 %.</p></div><div><h3>Conclusion</h3><p>This study shows <em>Carica papaya</em> in a new light as a potential material that could be formulated into a natural product-based fungicide for combating <em>Phytophthora</em>-induced black pod disease.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100082"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000177/pdfft?md5=f2f79eabd8bcdc8aa7d3dd0aaac17ec4&pid=1-s2.0-S2773078624000177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}