Journal of Natural Pesticide Research最新文献

{"title":"Larvicidal potential of ether extract, and its derivatives fatty acids, and fatty acid methyl esters from Tecoma stans seeds: Bioprospecting for an effective insecticide","authors":"Lucas S. Azevedo ,&nbsp;Thaís P.R. Gonçalves ,&nbsp;Mairon C. Coimbra ,&nbsp;Ezequias P. de Siqueira ,&nbsp;Stênio N. Alves ,&nbsp;Luciana A. R. dos S. Lima","doi":"10.1016/j.napere.2024.100087","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100087","url":null,"abstract":"<div><p>In the work, the larvicidal potential of ether extract (EE), and its hydrolyzed products enriched with fatty acids (FA), and fatty acid methyl esters (FAME) obtained from <em>Tecoma stans</em> seeds was evaluated. EE, and FAME were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and three samples were evaluated in a preliminary screen for larvicidal activity against <em>Culex quinquefasciatus</em> at concentrations of 31.25, 62.5, 125, 250, 500, and 1000 μg/mL. The larvicidal efficacy of all samples was assessed at 24, 48, and 144 h post-treatment, detecting the movement of each larva. The data obtained were subjected to probit analysis to determine the lethal concentrations that killed 50 % and 90 % (LC₅₀ and LC₉₀) of the treated larvae. In EE, the phytosterol γ-sitostenone was characterized as the predominant component (30.40 %), while in FAME the presence of methyl palmitate (25.01 %), and methyl stearate (22.14 %) stood out. Notable larvicidal activity was detected after 24 h of treatment. EE exhibited moderate toxicity against <em>C. quinquefasciatus</em> (LC₅₀ = 193.34 μg/mL), while FAME was slightly toxic (LC₅₀ = 269.43 μg/mL), and FA, the most promising product, presented an LC₅₀ = 20.06 μg/mL. The results together demonstrate that extracts, and hydrolyzed products from <em>T. stans</em> seeds may be useful as eco-friendly, economical, and effective larvicidal agents. However, additional studies are needed to search for bioactive compounds of plant extracts, and their derivatives that exhibit larvicidal activity, and their modes of action.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000220/pdfft?md5=0239c6a255e19843fb4ebeabf1e8d7fe&pid=1-s2.0-S2773078624000220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals, UPLC-QTOF-MS/MS analysis, potential acaricidal activity and molecular modeling of Datura metel grown in North-Eastern India","authors":"Aditi Kundu ,&nbsp;Karivaradharajan Swarnalakshmi ,&nbsp;Sushmita Rajkhowa ,&nbsp;Anirban Barik ,&nbsp;S.N. Bhagyasree ,&nbsp;Tankeswar Nath ,&nbsp;Bidyut Kumar Sarmah ,&nbsp;Srikant Ghosh","doi":"10.1016/j.napere.2024.100086","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100086","url":null,"abstract":"<div><p>The present investigation emphasized exploitation of <em>Datura metel</em> L. for potential acaricidal activity. Extraction method suggested higher extraction efficiency with the use of 50 % methanol over aqueous medium. UPLC-QTOF-ESI-MS analysis of the extracts resulted tentative identification of eighteen tropane alkaloids such as tigloidin, hyoscyamine N-oxide, scopolamine N-oxide, hyocyamine, hydroxyapoatropine, tropinone, scopine, hyoscine, atropine, valtropine, ditigloyloxytropane, ditigloyloxytropanol, apohyoscine, tigloyloxytropane, norhyoscine, meteloidine, cuscohygrine and tropine in the aerial parts (EAP) and roots (ER). Further, nine phenolic acids such as gallic, protocatechuic, chlorogenic, vanillic, p-coumaric, ferulic, quinic, syringic, and sinapic acids along with fourteen flavonoids such as luteolin-7-glucoside, catechin-3-O-rhamnoside, kempferol-3,7-O-diglucoside, naringenin-6-β-D-glucopyranoside, quercetin 3-O-glucosyl-xyloside, apigenin-7-O-glucoside, flavonol-3-glucoside, luteolin, epicatechin, chrysoeriol, diadzein, genistein, hesperidin and hydroxycoumarin were also identified. Highest acaricidal activity was observed for ER showing LC₅₀ and LC₉₀ of 112.5 and 317.3 μg/mL after 48 h of exposure, respectively. Molecular modeling analysis revealed favourable interaction of tigloidine with UDP transferase of <em>T. urticae</em> through attractive charge, pi-alkyl, alkyl and conventional H bonds. Though, ER was found to be phytotoxic with the highest root growth inhibition in terms of IC₅₀ value of 131.2 and 184.9 μg/mL against <em>Chenopodium album</em> and <em>Triticum aestivum</em>, respectively. Being eco-benign natural products, <em>Datura</em> phytochemicals can serve as potential bio-acaricide, thereby, a smart delivery system could be developed comprising synergistic concoction of the alkaloid rich phytochemical fractions for efficient management of the notorious acarids.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000219/pdfft?md5=c9ca56be676733a1d772d86cfeba1af7&pid=1-s2.0-S2773078624000219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141434959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of essential oils from six Moroccan plants against the causal agent of Verticillium wilt of olives","authors":"Rachid Azenzem ,&nbsp;Tayeb Koussa ,&nbsp;Mohamed Najib Alfeddy","doi":"10.1016/j.napere.2024.100085","DOIUrl":"10.1016/j.napere.2024.100085","url":null,"abstract":"<div><p>In the realm of sustainable agriculture, biocontrol is gaining considerable attention for its focus on the development of safe, biodegradable, and environmentally friendly alternatives to traditional biocides. This study explored the potential use of essential oils as alternative biocontrol products for managing <em>Verticillium dahliae</em>, the causal agent of Verticillium wilt in olive trees. Essential oils (EOs) were obtained through hydrodistillation from six Moroccan aromatic and medicinal plants (<em>Ammodaucus leucotrichus</em>, <em>Mentha spicata</em>, <em>Origanum majorana</em>, <em>Rosmarinus officinalis</em>, <em>Salvia officinalis</em>, and <em>Thymus satureioides</em>). These EOs were then subjected to chemical analysis by gas chromatography-mass spectrometry (GC-MS) and evaluated in vitro for their antifungal properties against <em>V. dahliae</em>. The bioassays revealed that all tested EOs reduced mycelial growth of the studied strain, and tended to have dose-dependent effects. Compared with the various essential oils evaluated in this study, <em>A. leucotrichus</em> essential oils showed the most pronounced antifungal efficacy at low and moderate concentrations. These EOs stand out for their high content of two monoterpenes, perillia aldehyde (84.19 %) and limonene (14.15 %), which were not found in the other essential oils examined. Therefore, our results open up new possibilities for exploiting these plants and their chemical constituents to develop a natural biofungicide as a component of integrated management strategies for Verticillium wilt of olive and other crops.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000207/pdfft?md5=e608234ce3fb5cfd3c85a4f372129787&pid=1-s2.0-S2773078624000207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some aspects concerning citronella grass essential oil and the agroecological approach to protecting stored cowpea beans","authors":"Marcela de Souza Alves ,&nbsp;Elisabeth Alves Duarte Pereira de Medeiros ,&nbsp;Camila da Silva Barbosa Pereira ,&nbsp;Cristiane Martins Cardoso ,&nbsp;Emerson Guedes Pontes ,&nbsp;André Marques dos Santos ,&nbsp;Marco Andre Alves de Souza","doi":"10.1016/j.napere.2024.100084","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100084","url":null,"abstract":"<div><p>This study aimed to determine the impact of hydrodistillation duration on the chemical composition of citronella-grass essential oil. Furthermore, the oil's efficacy against cowpea weevil was evaluated by carrying out biological activity tests. The essential oil was obtained and chemically characterized. Subsequently, it was tested at different concentrations to evaluate its impact on the reproductive cycle and the total activity of the insects' biotransformation enzymes. Oxygenated monoterpene comprised the majority of the substances present. The mathematical model fitted to the extraction kinetics predicts that after 60 minutes of hydrodistillation, approximately 96% of essential oil, 94% of monoterpenes, and 50% of sesquiterpenes will be obtained with confidence. The fumigating effect of the citronella-grass essential oil resulted in increased mortality, reduced oviposition, and lower emergence of adults. The essential oil at sublethal concentration (0.09 mg cm^-3 for 6 hours) significantly reduced the total activity of biotransformation enzymes (acetylcholinesterase, α- and β-esterase, carboxylesterase) in female cowpea weevils.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100084"},"PeriodicalIF":0.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000190/pdfft?md5=94f2e0f4b0c29d471d1be1b5845f26f3&pid=1-s2.0-S2773078624000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141243612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-black pod disease activity of leaf extract of Carica papaya Linn","authors":"Tamekloe Woasiedem ,&nbsp;Ishmael Amoako-Attah ,&nbsp;Francis Ackah Armah ,&nbsp;Isaac Asiamah","doi":"10.1016/j.napere.2024.100082","DOIUrl":"10.1016/j.napere.2024.100082","url":null,"abstract":"<div><h3>Background</h3><p><em>Theobroma cacao (</em>Cocoa) is a major foreign exchange earner for Ghana. Over the recent decades, there has been a consistent decline in cocoa production in the country. A major contributing factor is the cocoa black pod disease caused by <em>Phytophthora</em> species. Synthetic fungicides have been used to control the pathogens over the years. However, recent calls for organic cocoa devoid of residues from synthetic fungicides, which are known to pose several health challenges as well as a negative impact on the environment, have gained attention. Thus, inviting investigations into more environmentally friendly and sustainable alternatives to synthetic fungicides.</p></div><div><h3>Objective</h3><p>This study explored the inhibitory potential of crude extract from <em>Carica papaya</em> against cocoa black pod disease caused by <em>Phytophthora</em> spp.</p></div><div><h3>Material and methods</h3><p>An <em>in-silico</em> study was done using known phytochemicals from <em>Carica papaya</em> as ligands against a <em>Phytophthora</em> effector protein. Crude extracts were obtained from the plant materials using 70 % ethanol as the extraction solvent. The crude Green Leaf Extract (GLE), Aging Induced Chlorophyll Deficient Leaf Extract (AICDLE), and Matured Black Seed Extract (SDE) were tested <em>in-vitro</em> against pre-cultured <em>P. palmivora</em> and <em>P. megakarya</em> using the poison food technique. GLE was modulated with Delco, a synthetic fungicide, at different ratios which were tested <em>in-vitro</em> and <em>in-vivo</em>.</p></div><div><h3>Results</h3><p>A total of 16 ligands showed binding affinity higher or equal to the standard ligand, alliin, suggesting <em>Carica papaya</em> as a repository of anti-phytophthora agents. Assessment of crude extracts at concentrations 5–20 mg/ml showed fungistatic activity against <em>P. palmivora</em> and <em>P. megakarya in-vitro</em>. Modulation of GLE with Delco produced some combinations that made GLE fungicidal <em>in-vitro</em> against <em>P. palmivora</em> and <em>P. megakarya</em> with fractional inhibition concentration indices between 0.51 and 0.65, interpreted as partially synergistic. The <em>in-vivo</em> assessment of combination D (Delco and GLE in a 0.3 mg:3 mg per ml ratio) performed as well as Delco alone at the recommended dose of 5 mg/ml. Thus, combination D reduces the requirement for copper-based fungicide by over 90 %.</p></div><div><h3>Conclusion</h3><p>This study shows <em>Carica papaya</em> in a new light as a potential material that could be formulated into a natural product-based fungicide for combating <em>Phytophthora</em>-induced black pod disease.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100082"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000177/pdfft?md5=f2f79eabd8bcdc8aa7d3dd0aaac17ec4&pid=1-s2.0-S2773078624000177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical screening and antimicrobial activities of Guizotia abyssinica L. leaf and flower extracts","authors":"Priyanka Prasad ,&nbsp;Aadil Mansoori ,&nbsp;Nisha Prajapati ,&nbsp;Jyotima Tripathi ,&nbsp;Kapil Sharma ,&nbsp;Anirudh Kumar ,&nbsp;Subha Narayan Das","doi":"10.1016/j.napere.2024.100083","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100083","url":null,"abstract":"<div><p>Sustainable agricultural systems demand less or no use of synthetic chemicals, which have various adverse effects on the environment and human health. In this context, studies on phytochemicals with antimicrobial and antioxidant properties hold great promise to suppress plant diseases and, thereby, reducing the application of synthetic chemical pesticides. Such an alternative approach requires screening and identification of bioactive compounds from various plant species. In the present study, <em>Guizotia abyssinica</em> L., an oilseed crop plant that usually requires less water with no pesticide inputs to grow, was screened for its potential antioxidant and antimicrobial phytochemicals. The methanolic and aqueous extracts of leaves and flowers of <em>G. abyssinica</em> were tested for their polyphenol content (total phenols and flavonoids) and antioxidant potential. The antioxidant and free radical scavenging activities of both methanolic and aqueous extracts were higher in comparison to many previously reported plant extracts. Furthermore, leaf extracts of <em>G. abyssinica</em> have shown slightly higher antioxidant activity than flower extracts. <em>In vitro</em> antimicrobial studies confirmed the inhibitory effect of methanolic extracts of leaves and flowers against phytopathogens like <em>Xanthomonas oryzae</em> RR24, <em>Fusarium</em> sp. and <em>Alternaria</em> sp. The chemical nature and composition of these crude extracts were analyzed by Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GC-MS). Approximately, 28 bioactive compounds detected in GC-MS were predicted to have antimicrobial activity. Based on our results, we conclude that the extracts derived from <em>G. abyssinica</em> have demonstrated remarkable efficacy in inhibiting the proliferation of phytopathogens, thus exhibiting immense potential in promoting sustainable agricultural practices.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000189/pdfft?md5=c351619151207cfe6d1cb8c3b2ba1906&pid=1-s2.0-S2773078624000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140918823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding missing Declaration of Competing Interest statements in previously published articles","authors":"","doi":"10.1016/j.napere.2024.100081","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100081","url":null,"abstract":"","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"8 ","pages":"Article 100081"},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000165/pdfft?md5=2955738313c800237aeab3948e08a571&pid=1-s2.0-S2773078624000165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140807861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biopesticide potential of Anisomeles indica Kuntze leaf essential oil and its nanoemulsion against bacterial blight - Xanthomonas strains","authors":"M. Savitharani,&nbsp;Vadlapudi Kumar,&nbsp;T. Manjunatha,&nbsp;F. Ruksana,&nbsp;E. Vishala,&nbsp;H.M. Prathap","doi":"10.1016/j.napere.2024.100075","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100075","url":null,"abstract":"<div><p>The objective of this study was to examine the chemical composition of essential oil <em>A. indica</em> leaves and its nanoemulsion, and evaluation of potential for use as a biopesticide. Essential oil of <em>A. indica</em> extracted from fresh leaves was found to be 99.97% composed of 59 components, according to GC-MS analysis. A non-ionic surfactant Tween 80 and the essential oil of <em>A. indica</em> were combined to create a stable nanoemulsion using sonication method. A long durable stable nanoemulsion was obtained after thirty minutes of sonication, and it was examined using TEM (Transmission electron microscopy), DLS (Dynamic light scattering), and FTIR (Fourier transform infrared spectroscopy) analyses. To consider its practical applications, other factors such as stability, viscosity, pH, turbidity, and creaming index were also investigated. Antibacterial properties of essential oil and nanoemulsion were evaluated against bacterial blight plant pathogens <em>Xanthomonas oryzae</em> and <em>Xanthomonas citri</em>. The MIC (Minimum inhibitory concentration) was determined using the broth microdilution method, and the anti-<em>Xanthomonas</em> activity was assessed utilizing by agar disc diffusion method. Using the crystal violet (CV) technique, inhibition of biofilm formation and development were evaluated. Disturbance of bacterial membrane permeability was confirmed by estimating the proteins and nucleic acids (DNA, and RNA) leakage from the cell membrane. Greenhouse studies using rice/paddy plants were conducted to demonstrate the antibacterial potential of essential oil and nanoemulsion, using RNR-15048 rice cultivar by seed treatment and foliar spray groups. Results of the investigations suggest that, when the essential oil was transformed into a nanoemulsion, its antibacterial effect against bacteria was significantly increased, that might be attributed to the essential oil enhanced ability to penetrate the bacterial cells. Essential oil of <em>A. indica</em> and its nanoemulsion reduced rice bacterial blight disease by 84%and pathogen-virulence by 92%, and promoted the growth and development of rice/paddy seedlings. These results confirm that <em>A. indica</em> essential oil and its nanoemulsion possess anti-bacterial blight properties, without affecting growth and development of host plants. Essential oil of <em>A. indica</em> and its nanoemulsion could be used as biopesticide to control <em>Xanthomonas</em> induced bacterial blight diseases in crop plants either by using for seed treatment or as a foliar spray.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"8 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000098/pdfft?md5=1469203dc70307ed70514e5388f2e3de&pid=1-s2.0-S2773078624000098-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140604809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-pesticidal potential of the crude extract of Andrographis paniculata [(Burm. F.) Wall ex. Nees] in the agricultural field: A review","authors":"Kirti Verma ,&nbsp;Saudan Singh","doi":"10.1016/j.napere.2024.100076","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100076","url":null,"abstract":"<div><p>Kalmegh [<em>Andrographis paniculata (Burm. F.) Wall ex. Nees</em>] is one of the significant medicinal plants which possess active ingredients of therapeutic potential, widely used in the treatment of anti-inflammatory, anti-bacterial, anti-oxidant, hepatoprotective, etc. The plant is widely used in the Ayurvedic and homeopathic systems of medicine. In spite of this, kalmegh exhibits insecticidal and repellent properties against pests and insects of important medicinal significance and can be used as a promising bio-insecticide/pesticide. All the useful collective information and the database of the review are generated from the previously published articles using Web of Sciences, PubMed, Google Scholar, and other web searches. It possesses biopesticidal/insecticidal properties such as suppressing feeding activity, anti-oviposition activity, and weedicide of <em>Plutella xylostella</em>, <em>Spilaractia obliqua, Callosobruchus chinesis L., Nephotettix cincticeps, Helicoverpa armigera, Parthenium hysterophorus,</em> etc. Thus this review will provide some positive aspects towards the research for exploration and further studies in the future. This review outlines the summary of factual records of previous research concerning its ethnobotany, phytoconstituents, and bio-insecticidal/pesticidal potential of the crop. However, in the research sector, <em>Andrographis paniculata</em>'s prospects will be on an upswing, the demand of the crop burgeoning in the industries, and eventually, farmers also reaping its benefits.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"8 ","pages":"Article 100076"},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000104/pdfft?md5=81906e8bcce6159f68650fcb600087fa&pid=1-s2.0-S2773078624000104-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140344747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme production by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae and their application in the control of nematodes (Haemonchus spp. and Meloidogyne incognita) in vitro","authors":"Lisseth Bibiana Puentes Figueroa ,&nbsp;Ruth Celestina Condori Mamani ,&nbsp;Debora Castro de Souza ,&nbsp;Jhennifer Cristina de Souza Alves ,&nbsp;Stefany Amorim de Souza ,&nbsp;Cecilia Baldoino Ferreira ,&nbsp;Tiago Facury Moreira ,&nbsp;Willian César Terra ,&nbsp;Filippe Elias de Freitas Soares","doi":"10.1016/j.napere.2024.100077","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100077","url":null,"abstract":"<div><p>Brazil is affected by the infection of gastrointestinal nematodes (<em>Haemonchus</em> spp.) because it causes subclinical diseases in small ruminants that directly affect weight and milk production and, in turn, generate a health risk for the animals. In the same way root-knot nematodes (<em>Meloidogyne incognita</em>) is a serious disease, which parasitize the roots of tomato plants causing damage such as poor nutrient absorption leading to significant yield losses. In order to minimize the economic impact of these nematodes, it is important to establish new control strategies. <em>Beauveria bassiana</em> and <em>Metarhizium anisopliae</em> are two main mycoinsecticides used to control many orders of insects, such as Lepidoptera and Hemiptera. These fungi have the ability to produce extracellular enzymes, which play an important role to control in the pest infection process. The aim of this study was to evaluate the production of proteases and chitinases by the entomopathogenic fungi <em>B. bassiana</em> and <em>M. anisopliae</em> on different solid and liquid culture media and their application <em>in vitro</em> to the control of <em>Haemonchus</em> spp and <em>Meloidogyne incognita</em>. To achieve this goal, enzymes were produced under solid and liquid fermentation conditions to determine the highest chitinolytic and proteolytic activity of commercial two strain of <em>B. bassiana</em> (IBCB 66 and ESALQ PL63) and <em>M. anisopliae</em> (IBCB 425 and ESALQ E9) in liquid media: SDY broth, YPG culture medium, synthetic, and soluble starch with yeast extract. The solid media tested were: rice supplemented with whey and chrysalis flour mixed with rice. In addition, the nematicidal action of their extracts (containing concentrated enzymes and without fungal cells) was measured. The results showed that the best culture medium (p&lt; 0.01) for protease was solid serum rice medium, with a value for <em>M. anisopliae</em> IBCB 425 of 52 U/mg, and for <em>B. bassiana</em> ESALQ PL63 the value was 36 U/mg, compared to all the tested media. On the other hand, for chitinase of <em>M. anisopliae</em> strain IBCB 425, the activity value was close to 0.60 U/mg. In contrast, for <em>B. bassiana</em> isolate ESALQ PL63, SDY medium was the best inducer for chitinase production, with a value of 0.90 U/mg. Regarding the nematicidal activity of the crude extracts, the reduction percentages were 58% for ESALQ PL63 and 100% for IBCB 425 in the case of animal parasites. For plant parasites, the reduction percentages were 19% for ESALQ PL63 and 71% for IBCB 425. Thus, the use of enzyme-rich crude extracts presents promising control options for pest control.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"8 ","pages":"Article 100077"},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000116/pdfft?md5=cb9b4767f05dfcdcf224795a12d22ce1&pid=1-s2.0-S2773078624000116-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140341062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}