Journal of Nanjing Medical University最新文献

{"title":"Effects of (−)-Epigallocatechin gallate on some protein factors involved in the epidermal growth factor receptor signaling pathway","authors":"Yinjiu Huang ,&nbsp;Ruiqing Xu ,&nbsp;Baoan Song ,&nbsp;Song Yang ,&nbsp;Li Zhao ,&nbsp;Shouwei Wu","doi":"10.1016/S1007-4376(09)60073-3","DOIUrl":"10.1016/S1007-4376(09)60073-3","url":null,"abstract":"<div><p>(−)-Epigallocatechin gallate (EGCG), a major polyphenolic constituent of green tea, can inhibit activity of specific receptor tyrosine kinases (RTKs) and related downstream signal transduction pathways, resulting in the control of unwanted cell proliferation. The epidermal growth factor receptor (EGFR) signaling pathway is one of the most important pathways that regulates growth, survival, proliferation and differentiation in mammalian cells. This review addresses the effects of EGCG on some protein factors involved in the EGFR signaling pathway in a direct or indirect manner. Based on our understanding of the interaction between EGCG and these factors, and based on their structures, EGCG could be used as a lead compound for designing and synthesizing novel drugs with significant biological activity.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 5","pages":"Pages 293-299"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60073-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82012542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis C virus NS3/4A with sequence variation at amino-terminus has different serine protease activities and inhibitory activities on IFN-β induction and p53-dependent transcriptional activation","authors":"Xueping Wanga ,&nbsp;Fujun Lib ,&nbsp;Motoko Nagano-Fujii ,&nbsp;Lin Deng ,&nbsp;Kikumi Kitayamac ,&nbsp;Hak Hottac","doi":"10.1016/S1007-4376(09)60066-6","DOIUrl":"10.1016/S1007-4376(09)60066-6","url":null,"abstract":"<div><h3>Objective</h3><p>To construct the point mutation plasmids expressing HCV NS3/4A with different secondary structures at the N-terminus, and to analyze their serine protease activities.</p></div><div><h3>Methods</h3><p>The point mutation plasmid constructs were generated by using the Quick Change site-directed mutagenesis kit with the backbone of M-H05-5 (A1-1), and were named as subgroup A1-2, A2-1, A2-2, B1-1, B1-2, B2-1, and B2-2 respectively. The transient expression of the constructs was investigated by immunofluorescence assay and Western blot analysis. The difference in in cis and in trans NS3 serine protease activity between each subgroup was determined by Western blot analysis. Luciferase reporter assay was used to observe the inhibitory effects of the constructs on RIG-I induced IFN-β promoter activity and on p53-dependent transcriptional activation.</p></div><div><h3>Results</h3><p>The point mutation plasmid constructs were verified for the correct sequence by DNA sequencing. The immunofluorescence assay revealed 4 subcellular localization patterns of NS3, including dot-like staining, diffuse staining, doughnut-like staining, and rod-shape staining. Western blot analysis indicated that the incomplete cleavage of NS3/4A appeared in subgroups A2-1 and B2-1, indicating that the in cis NS3 serine protease activities of subgroup A2-1 and B2-1 were weaker when compared with the other subgroups. By using NS5A/5BΔC as a substrate for NS3/4A serine protease, it was also found that the in trans NS3 serine protease activities of subgroup A2-1 and B2-1 were also weaker compared the other subgroups. Differences in inhibitory effects of HCV NS3 on RIG-I induced IFN-β promoter activity and on p53-dependent transcriptional activation were also observed between subgroup A2-1, B2-1 and the other subgroups.</p></div><div><h3>Conclusion</h3><p>The results suggest that subgroup A2-1 and B2-1 has weaker serine protease activities and weaker inhibitory activities on host cell functions than the other subgroups, which might be explained by the different secondary structure of the 120-aa sequence at N-terminus of NS3.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 257-264"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60066-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78436839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in the primary culture, purification and biological characteristics between endothelial cells and smooth muscle cells from rat aorta","authors":"Shaobo Hua ,&nbsp;Zifang Songa ,&nbsp;Qichang Zhenga ,&nbsp;Jun Nieb","doi":"10.1016/S1007-4376(09)60063-0","DOIUrl":"10.1016/S1007-4376(09)60063-0","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the differences of primary culture, purification and biological characteristics between endothelial cells and smooth muscle cells from rat aorta.</p></div><div><h3>Methods</h3><p>Endothelial cells were obtained using the vascular ring adherence, collagenase digestion method and an improved vascular ring adherence method, while smooth muscle cells were separated from tissue sections of rat aorta. Clones of endothelial cells were selected by limiting dilution assay. Both cell types were identified using specific cell immunofluorescent markers, and phase contrast microscopy was used to observe the morphological disparity between endothelial cells and smooth muscle cells at the single cell and colony level. Cell proliferation was determined by the cell counting kit-8. Differences between endothelial cells and smooth muscle cells were evaluated in trypsin digestion time, attachment time and recovery after cryopreservation.</p></div><div><h3>Results</h3><p>Endothelial cells were obtained by all three methods. The improved vascular ring method provided the most reproducible results. Cells were in good condition, and of high purity. Smooth muscle cells were cultured successfully by the tissue fragment culture method. Clonal expansion of single endothelial cells was attained. The two cell types expressed their respective specific markers, and the rate of proliferation of smooth muscle cells exceeded that of endothelial cells. Endothelial cells were more sensitive to trypsin digestion than smooth muscle cells. In addition, they had a shorter adherence time and better recovery following cryopreservation than smooth muscle cells.</p></div><div><h3>Conclusion</h3><p>The improved vascular ring method was optimal for yielding endothelial cells. Limiting dilution is a novel and valid method for purifying primary endothelial cells from rat aorta. Primary rat endothelial cell and vascular smooth muscle cell cultures exhibited different morphological characteristics, proliferation rate, adherence time, susceptibility to trypsin digestion and recovery after cryopreservation. Our research can be a good foundation for further application in the regeneration of blood vessel.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 241-246"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60063-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83800515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of NGF pretreatment on cerebral injury in gerbils","authors":"Yongxing Tan ,&nbsp;Junxiong Yu ,&nbsp;Difen Wang","doi":"10.1016/S1007-4376(09)60067-8","DOIUrl":"10.1016/S1007-4376(09)60067-8","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the effects and underlying mechanisms of different doses of nerve growth factor(NGF) pretreatment on neuron apoptosis and the expressions of the apoptosis-related protein, Bcl-2 and Bax, in the gerbil cerebral prefrontal cortex following global cerebral ischemia-reperfusion(I/R) injury.</p></div><div><h3>Methods</h3><p>Fifty-four gerbils were randomly divided into five groups, group C: sham operation(<em>n</em> = 6); group I/R(<em>n</em> = 12), group L(<em>n</em> = 12): low-dose NGF+I/R, group M(<em>n</em> = 12): medium-dose NGF+I/R and group H (<em>n</em> = 12): high-dose NGF+I/R. Groups I/R, L, M and H were further divided into 2 subgroups according to the duration of reperfusion(24 h and 72 h). The global cerebral I/R injury model was induced by bilateral carotid artery occlusion for 20 min, followed by removal of the clamps to permit reperfusion. In groups L, M and H, NGF was injected into the lateral ventricle 24 h prior to ischemia. Terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling(TUNEL) and immunohistochemical staining were performed to detect neuron apoptosis and the expressions of Bcl-2 and Bax protein in the cerebral cortex, respectively.</p></div><div><h3>Results</h3><p>In the I/R group and NGF pretreatment groups(L, M and H groups), reperfusion for 72 h caused higher percentages of both neurons exhibiting apoptosis and Bax positive cells(<em>P</em> &lt; 0.01), but lower percentages of Bcl-2 positive cells compared with the corresponding 24 h reperfusion groups(<em>P</em> &lt; 0.05). All NGF pretreatment groups exhibited lower percentages of neurons exhibiting apoptosis and Bax positive cells but a higher percentage of Bcl-2 positive-cells relative to the I/R group(<em>P</em> &lt; 0.01). Moreover, the high-dose NGF pretreatment group had a greater decreased neuronal apoptosis and Bax protein expression and increased Bcl-2 protein expression than either the low-or medium-dose groups.</p></div><div><h3>Conclusion</h3><p>Neuron apoptosis participates in the progression of cerebral ischemia-reperfusion injury. The protective effect of NGF pretreatment against ischemia-reperfusion-induced neuron apoptosis seems to be both time- and dose-dependent. This anti-apoptosis mechanism may be associated with upregulation of Bcl- 2 protein expression and downregulation of Bax protein expression.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 265-269"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60067-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86982323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells","authors":"Aihua Yuan ,&nbsp;Zongfang Li ,&nbsp;Xinqiu Li ,&nbsp;Shanyong Yi ,&nbsp;Shukui Wang ,&nbsp;Kaiwang Shi ,&nbsp;Jianmin Bian","doi":"10.1016/S1007-4376(09)60071-X","DOIUrl":"10.1016/S1007-4376(09)60071-X","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the stimulatory effect of epinephrine(Epi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells.</p></div><div><h3>Methods</h3><p>The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with β adrenergic receptor(β2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERK½ intracellular activity.</p></div><div><h3>Results</h3><p>Epi transiently activated MAPK/ERK½ in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK½.</p></div><div><h3>Conclusion</h3><p>Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tumor cells by inhibiting the β2-AR-mediated activation of MAPK/ERK½.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 284-288"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60071-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87082144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen and its signaling pathway in non-small cell lung cancer(NSCLC)","authors":"Ruitong Xu,&nbsp;Yongqian Shu","doi":"10.1016/S1007-4376(09)60059-9","DOIUrl":"10.1016/S1007-4376(09)60059-9","url":null,"abstract":"<div><p>Lung cancer is the most common cancer in the world. It is a highly lethal disease in women and men, and new treatments are urgently needed. Several studies have implicated a role of estrogens and estrogen receptors in lung cancer progression. This review will investigate the biological significance of estrogens in lung cancer cells, the expression and molecular mechanisms of estrogen receptors(ER β, elucidate the prognostic significance of estrogens and their receptors in lung carcinomas and provide new options for patients afflicted with lung malignancies.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 217-223"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60059-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73712030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells","authors":"Fei Wang,&nbsp;Shushu Zhu,&nbsp;Xuefu Han,&nbsp;Jindan Xu,&nbsp;Jinnan Zhang,&nbsp;Di Yang","doi":"10.1016/S1007-4376(09)60064-2","DOIUrl":"10.1016/S1007-4376(09)60064-2","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the expression of OX40 ligand(OX40L) on C-reactive protein(CRP)-triggered mouse aorta endothelial cells (MAECs) <em>in vitro</em>.</p></div><div><h3>Methods</h3><p>MAECs from aorta were isolated by digestion with collagenase typeII. The cell growth was confirmed by morphological characteristics and the immunological marker, factor VIII(or Willebrand factor, vWF). The expression of OX40L by MAECs was detected by RT-PCR and western blot after incubating with 100μg/ml CRP for 48 hours.</p></div><div><h3>Results</h3><p>Twenty-day cultures of MAECs formed confluent monolayer of a cobblestone pattern. RT-PCR and western blot assay showed that the level of OX40L expression in MAECs receiving CRP treatment was higher than control.</p></div><div><h3>Conclusion</h3><p>A reliable method is described to isolate and propagate MAECs. CRP upregulates OX40L expression in MAECs.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 247-250"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60064-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76141283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes of the expression of CCNL2 gene during the differentiation of P19 cells to cardiac myocytes","authors":"Zhiwen Jiang,&nbsp;Lili Zhuo,&nbsp;Jie Gong,&nbsp;Rong Yang,&nbsp;Yanhui Sheng,&nbsp;Xiangqing Kong,&nbsp;Kejiang Cao","doi":"10.1016/S1007-4376(09)60069-1","DOIUrl":"10.1016/S1007-4376(09)60069-1","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the changes of cyclin L2(CCNL2) gene mRNA and protein during the differentiation of P19 cells to cardiac myocytes, and to explore the relationship between CCNL2 gene and the differentiation of cardiac myocytes.</p></div><div><h3>Methods</h3><p>P19 cells were cultured with 0.9% DMSO for 18 days. Western blots of cardiac troponin I (cTnI) were used to identify cell differentiation. Total RNA was extracted from P19 cells during the process of differentiation at various time points:pre-differentiation(Day 0), and Day 1 to Day 18. The expression levels of CCNL2 gene mRNA and protein were evaluated by RT-PCR and Western blot, respectively.</p></div><div><h3>Results</h3><p>After being induced to differentiate by DMSO for 4 days in suspension, spontaneously and rhythmically beating cells were seen at 8 day, which were cTnI-positive. In P19 cells, both the expression level of CCNL2 gene mRNA and protein were gradually down-regulated.</p></div><div><h3>Conclusion</h3><p>Both the expression of CCNL2 gene and protein were down-regulated during the process of the differentiation of P19 cells into cardiac myocytes, suggesting a possible role for this cyclin in their differentiation.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 275-278"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60069-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77953147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of adiponectin genotype polymorphisms at positions 45 and 276 with obstructive sleep apnea hypopnea syndrome","authors":"Mei Su,&nbsp;Xilong Zhang,&nbsp;Shicheng Su","doi":"10.1016/S1007-4376(09)60070-8","DOIUrl":"10.1016/S1007-4376(09)60070-8","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the relationship between adiponectin genotype polymorphisms and obstructive sleep apnea hypopnea syndrome (OSAHS).</p></div><div><h3>Methods</h3><p>Using the TaqMan polymerase chain reaction(PCR) method, the single nucleotide polymorphisms(SNP) at positions 45 and 276 in the adiponectin gene were determined in Chinese of the Han nationality in the Nanjing district. The OSAHS group consisted of 78 patients, and the control group contained 40 subjects. The association of adiponectin genotype polymorphisms at positions 45 and 276 with obstructive sleep apnea hypopnea syndrome was analyzed.</p></div><div><h3>Results</h3><p>No evidence of a direct association was found between OSAHS and adiponectin genotype SNP at positions 45 and 276(<em>P</em> &gt; 0.05). However, compared with those OSAHS patients having G/T+T/T genotype at position 276, the OSAHS patients with the G/G genotype showed a greater neck circumference(NC), a prolonged duration of the longest apnea event, and elevated levels of blood cholesterol and low-density lipoprotein cholesterol(<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>No direct association was detected between OSAHS and adiponectin genotype distribution at positions 45 and 276 in Chinese of Han nationality in the Nanjing district. However, OSAHS patients with the adiponectin G/G genotype at position 276 had a larger NC and the longest apnea event compared to those having the adiponectin SNP276 G/T +T/T genotype. This may have an indirect influence on the development of OSAHS,</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 279-283"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60070-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77963600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis advances in vascular cognitive impairment","authors":"Hua Zhou,&nbsp;Zhong Zhao","doi":"10.1016/S1007-4376(09)60060-5","DOIUrl":"10.1016/S1007-4376(09)60060-5","url":null,"abstract":"<div><p>Vascular cognitive impairment(VCI) encompasses the entire range of cognitive deficits associated with cerebrovascular disease(CVD), from mild deficits with little or no functional impairment, such as vascular cognitive impairment-no dementia(VCIND), to full-blown vascular dementia(VaD). Accurate diagnosis of vascular cognitive impairment is important but may be difficult. In this review we report advances in VCI in the following areas: etiology, subtypes, neuropsychology, biomarkers, neuroimaging, and diagnostic criteria.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 224-227"},"PeriodicalIF":0.0,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60060-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86464414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}