Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells

Journal of Nanjing Medical University Pub Date : 2009-07-01 DOI:10.1016/S1007-4376(09)60071-X

Aihua Yuan , Zongfang Li , Xinqiu Li , Shanyong Yi , Shukui Wang , Kaiwang Shi , Jianmin Bian

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Abstract

Objective

To investigate the stimulatory effect of epinephrine(Epi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells.

Methods

The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with β adrenergic receptor(β2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERK½ intracellular activity.

Results

Epi transiently activated MAPK/ERK½ in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK½.

Conclusion

Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tumor cells by inhibiting the β2-AR-mediated activation of MAPK/ERK½.

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菊花菊提取物可减弱肾上腺素对人肝癌细胞的有丝分裂作用

目的探讨肾上腺素(Epi)对人肝癌细胞生长的刺激作用及菊花提取物(CILE)对Epi诱导的肝癌细胞生长的拮抗作用。方法采用β肾上腺素能受体(β2- ar)阻断剂、MEK1/MEK2抑制剂和细胞内MAPK/ERK 1/ 2活性评估的增殖实验，研究Epi对HepG2和MHCC97H细胞的刺激作用和CILE对细胞生长的抑制作用。结果sepi瞬间激活HepG2和MHCC97H细胞中的MAPK/ERK 1 / 2，导致细胞生长爆发。Epi的作用被ICI 118551和U0126显著减弱。CILE显示Epi对两种细胞系生长的刺激作用呈剂量依赖性衰减，并抑制Epi诱导的MAPK/ERK½的激活。结论Epi可模拟有丝分裂原，刺激HepG2和MHCC97H细胞的生长，CILE可通过抑制β2- ar介导的MAPK/ERK 1 / 2的激活，有效减弱Epi对肿瘤细胞的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Nanjing Medical University

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