Journal of Nanjing Medical University最新文献

{"title":"ELABELA protects against diabetic kidney disease by activating high glucose-inhibited renal tubular autophagy","authors":"Xiyin Zheng, Lulu Yin, Jing Song, Juan Chen, Wensha Gu, Min Shi, Hong Zhang","doi":"10.7555/jbr.36.20220214","DOIUrl":"https://doi.org/10.7555/jbr.36.20220214","url":null,"abstract":"ELABELA (ELA), an endogenous ligand for the apelin receptor (APJ), has been observed to decrease in the plasma of patients with diabetic kidney disease (DKD). The current study explored potential function and underlying mechanisms of ELA in DKD. Our findings revealed that the ELA levels were decreased in the kidneys of DKD mice. ELA administration mitigated renal damage and downregulated the expression of fibronectin (FN), collagen Ⅳ (Col-Ⅳ), and transforming growth factor-β1 (TGF-β1) in the <i>db/db</i> mice and high glucose cultured HK-2 cells. Furthermore, the autophagy markers, the Beclin-1 and LC3-Ⅱ/Ⅰ ratio, were significantly impaired in DKD, but the ELA treatment reversed these alterations. Mechanistically, the inhibitory effects of ELA on the secretion of fibrosis-associated proteins in high glucose conditions were blocked by pretreatment with 3-methyladenine (3-MA, an autophagy inhibitor). It is likely that ELA protected against renal damage in the <i>db/db</i> mice and high glucose-induced HK-2 cell injury through the activation of renal tubular autophagy. In conclusion, ELA may effectively protect against DKD by activating high glucose-inhibited renal tubular autophagy.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135894245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Irisin/BDNF signaling in the muscle-brain axis and circadian system: a review","authors":"None Alexey N. Inyushkin, None Vitalii S. Poletaev, None Elena M. Inyushkina, None Igor S. Kalberdin, None Andrey A. Inyushkin","doi":"10.7555/jbr.37.20230133","DOIUrl":"https://doi.org/10.7555/jbr.37.20230133","url":null,"abstract":"In mammals, the timing of physiological, biochemical and behavioral processes over a 24-h period is controlled by circadian rhythms. To entrain the master clock located in the suprachiasmatic nucleus of the hypothalamus to a precise 24-h rhythm, environmental zeitgebers are used by the circadian system. This is done primarily by signals from the retina via the retinohypothalamic tract, but other cues like exercise, feeding, temperature, anxiety, and social events have also been shown to be non-photic zeitgebers. The recently identified myokine irisin is proposed to serve as an entraining non-photic signal of exercise. Irisin is a product of cleavage and modification from its precursor membrane fibronectin type Ⅲ domain-containing protein 5 (FNDC5) in response to exercise. Apart from well-known peripheral effects, such as the “browning\" of white adipocytes, irisin can penetrate the blood-brain barrier and display the effects on the brain. Experimental data suggest that FNDC5/irisin mediates the positive effects of physical activity on brain functions. In several brain areas, irisin induces the production of brain-derived neurotrophic factor (BDNF). In the master clock, a significant role in gating photic stimuli in the retinohypothalamic synapse for BDNF is suggested. However, the brain receptor for irisin is unknown yet. In the current review, the interactions of physical activity and the irisin/BDNF axis with the circadian system are reconceptualized.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135838786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of sevoflurane on left ventricular function by speckle-tracking echocardiography in coronary bypass patients: A randomized trial","authors":"None Chanjuan Gong, None Xiaokai Zhou, None Yin Fang, None Yanjuan Zhang, None Linjia Zhu, None Zhengnian Ding","doi":"10.7555/jbr.37.20230173","DOIUrl":"https://doi.org/10.7555/jbr.37.20230173","url":null,"abstract":"The present study aimed to dynamically observe the segmental and global myocardial movements of the left ventricle during coronary artery bypass grafting (CABG) using transesophageal speckle-tracking echocardiography, and to assess the impact of sevoflurane on cardiac function. Sixty-four patients scheduled for the off-pump CABG were randomly divided into a sevoflurane-based anesthesia (AS) group and a propofol-based total intravenous anesthesia (AA) group. The AS group demonstrated a higher absolute value of left ventricular (LV) global longitudinal strain (GLS) than the AA group at both T₁ (after harvesting all grafts and before coronary anastomosis) and T₂ (30 min after completing all coronary anastomoses) (<i>P</i> < 0.05). Moreover, strain improvement in the segment with the highest preoperative strain was significantly reduced in the AS group, compared with the AA group at both T₁ and T₂ (<i>P</i> < 0.01). The flow of the left internal mammary artery-left anterior descending artery graft was superior, and the postoperative concentration of troponin T (cTnT) decreased rapidly in the AS group compared with the AA group (<i>P</i> < 0.05). Compared with total intravenous anesthesia, sevoflurane resulted in significantly higher GLS, stroke volume, and cardiac output. Sevoflurane also led to an amelioration in the condition of the arterial graft. Furthermore, sevoflurane significantly reduced strain improvement in the segmental myocardium with a high preoperative strain value. The findings need to be replicated in large studies.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135446424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylated protein chip combined with artificial intelligence tools for precise drug screening","authors":"None Katsuhisa Horimoto, None Yuki Suyama, None Tadamasa Sasaki, None Kazuhiko Fukui, None Lili Feng, None Meiling Sun, None Yamin Tang, None Yixuan Zhang, None Dongyin Chen, None Feng Han","doi":"10.7555/jbr.37.20230082","DOIUrl":"https://doi.org/10.7555/jbr.37.20230082","url":null,"abstract":"We have developed a protein array system, named “Phospho-Totum”, which reproduces the phosphorylation state of a sample on the array. The protein array contains 1 471 proteins from 173 known signaling pathways. According to the activation degrees of tyrosine kinases in the sample, the corresponding groups of substrate proteins on the array are phosphorylated under the same conditions. In addition to the measured phosphorylation levels of the 1 471 substrates, we developed and performed the AI-assisted tools to further characterize the phosphorylation state and estimate pathway activation, tyrosine kinase activation, and a list of kinase inhibitors that produce phosphorylation states similar to that of the sample. The Phospho-Totum system, which seamlessly links and interrogates the measurements and analyses, has the potential to not only elucidate pathophysiological mechanisms in diseases, by reproducing the phosphorylation state of samples, but also be useful for drug discovery, particularly for screening targeted kinases for potential drug kinase inhibitors.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135594181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of α-ENaC induces pancreatic β-cell dysfunction, ER stress, and SIRT2 degradation","authors":"None Xue Zhang, None Dan Zhang, None Lei Huo, None Xin Zhou, None Jia Zhang, None Min Li, None Dongming Su, None Peng Sun, None Fang Chen, None Xiubin Liang","doi":"10.7555/jbr.37.20230128","DOIUrl":"https://doi.org/10.7555/jbr.37.20230128","url":null,"abstract":"Islet beta cells (β-cells) produce insulin in response to high blood glucose levels, which is essential for preserving glucose homeostasis. Voltage-gated ion channels in β-cells, including Na⁺, K⁺, and Ca²⁺ channels, aid in the release of insulin. Epithelial sodium channel alpha subunit (α-ENaC), a voltage-independent sodium ion channel, is also expressed in human pancreatic endocrine cells. However, there has not been much study done on ENaC's function in β-cells. In the current work, we found that human pancreatic glandule and pancreatic islet β-cells expressed α-ENaC. In the pancreas of <i>db/db</i> mice, high-fat diet-induced obesity, and in mouse islet β-cells (Min6 cells) treated with palmitate, α-ENaC expression was increased. When α-ENaC was overexpressed in Min6 cells, insulin content and glucose-induced insulin secretion were markedly reduced. On the other hand, palmitate injured islet β-cells, suppressed insulin synthesis and secretion, and increased α-ENaC expression in Min6 cells. However, α-ENaC knockout (<i>Scnn1a</i>^-/-) in Min6 cells attenuated β-cells disorder induced by palmitate. Furthermore, we revealed that α-ENaC regulated the ubiquitylation and degradation of Sirtuin 2 in β-cells. α-ENaC also modulated β-cell function related to inositol-requiring enzyme 1alpha/X-box-binding protein-1 (IRE1α/XBP1) and protein kinase RNA-like endoplasmic reticulum kinase/C/EBP homologous protein (PERK/CHOP) endoplasmic reticulum stress pathways. These results suggest that α-ENaC plays a novel role in insulin synthesis and secretion in β-cells. Upregulation of α-ENaC promotes islet β-cell dysfunction. As a result, α-ENaC is a key regulator involved in islet β-cell damage and a potential therapeutic target for type 2 diabetes mellitus.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135660469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful rescue of disseminated &lt;i&gt;Nocardia&lt;/i&gt; infection with multiple abscesses in a patient with membranous nephropathy after cardiopulmonary resuscitation: A three-year follow-up","authors":"None Yili Xu, None Hanyang Qian, None Wen Qian, None Li Dong, None Weiying Liu, None Yan Zhu, None Yaning Mei, None Yi Xu, None Ling Wang, None Yi Xia, None Xu Qi, None Huanping Mei, None Xueqiang Xu, None Huijuan Mao, None Changying Xing, None Ningning Wang","doi":"10.7555/jbr.37.20230107","DOIUrl":"https://doi.org/10.7555/jbr.37.20230107","url":null,"abstract":"Nocardiosis manifests as an opportunistic infection, primarily affecting individuals who are immunocompromised and susceptible to the infection. We present a case study of one patient with nephrotic syndrome and membranous nephropathy, who underwent treatment with prednisone and cyclosporine in 2016. In early 2017, the patient was diagnosed with a \"fungal infection\" and discontinued the use of cyclosporine. After anti-infection therapy for one month, a cranial magnetic resonance imaging scan showed multiple abscesses in the right temporal region. The diagnosis of nocardiosis was confirmed based on the presence of metastatic abscess masses, multiple lung and brain lesions, and a positive culture of <i>Nocardia</i> in the drainage. We changed the anti-infection therapy to a combination of trimethoprim-sulfamethoxazole (TMP-SMX), minocycline, and voriconazole. However, the patient experienced sudden cardiac arrest and subsequently recovered after cardiopulmonary resuscitation. During the five-month follow-up period following the discharge, the patient displayed an enhanced nutritional status and stable renal function. The focal infection ultimately resolved during the subsequent three years. Neuro-infection caused by <i>Nocardia</i> should be considered in immunocompromised patients, and TMP-SMX is the preferred initial therapy; however, given the high mortality rate, long-term combination therapy with imipenem, cefotaxime, amikacin and TMP-SMX is suggested.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136053666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer therapeutic strategies for targeting mutant p53-Y220C","authors":"None Vitaly Chasov, None Damir Davletshin, None Elvina Gilyazova, None Regina Mirgayazova, None Anna Kudriaeva, None Raniya Khadiullina, None Youyong Yuan, None Emil Bulatov","doi":"10.7555/jbr.37.20230093","DOIUrl":"https://doi.org/10.7555/jbr.37.20230093","url":null,"abstract":"The tumor suppressor p53 is a transcription factor with a powerful antitumor activity that is controlled by its negative regulator murine double minute 2 (MDM2, also termed HDM2 in humans) through a feedback mechanism. At the same time <i>TP53</i> is the most frequently mutated gene in human cancers. Mutant p53 proteins lose wild-type p53 tumor suppression functions and acquire new oncogenic properties among which are deregulated cell proliferation, increased chemoresistance, disruption of tissue architecture, promotion of migration, invasion and metastasis, and several other pro-oncogenic activities. The oncogenic p53 mutation Y220C, which accounts for over 100 000 cancer cases per year, creates an extended surface crevice in the DNA-binding domain destabilizing p53 and causing its denaturation and aggregation. This cavity can accommodate stabilizing small molecules that have therapeutic value. The development of suitable small-molecule stabilizers is one of the therapeutic strategies for reactivating the Y220C mutant protein. In this review we summarize approaches that target p53-Y220C, including reactivating this mutation with small molecules that bind Y220C hydrophobic pocket and developing immunotherapies as the goal of near future, which could target tumor cells that express the p53- Y220C neoantigen.","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136052987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the adjuvanticity of artemisinin with soluble Leishmania major antigens in BALB/c mice","authors":"Albert Kimutai ,&nbsp;Willy K. Tonui ,&nbsp;Michael M. Gicheru ,&nbsp;Peter Kamau Ngure ,&nbsp;Johnstone Ingonga ,&nbsp;Stella Kepha ,&nbsp;Laban Ireri Njeru ,&nbsp;Dorcas Wachira ,&nbsp;Robert Karanja Muhia ,&nbsp;Milkah Mwangi ,&nbsp;Lydia B. Nyamwamu","doi":"10.1016/S1007-4376(09)60084-8","DOIUrl":"10.1016/S1007-4376(09)60084-8","url":null,"abstract":"<div><h3>Objective</h3><p>To determine the adjuvant potential of artemisinin with a soluble leishmanial antigen in vaccinating BALB/c mice.</p></div><div><h3>Methods</h3><p>Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble leishmania antigens (SLA) alone, artemisinin co-administered with SLA, SLA and Bacille Calmette Guérin (BCG) vaccine, and artemisinin and BCG alone. Unvaccinated mice formed the control group. The induction of cell-mediated immunity following vaccination was determined by measuring <em>in vitro</em> lymphocyte proliferation and the production of interleukin (IL)-4, IL-5 and gamma interferon (IFN-γ) determined by flow cytometry. Protection against <em>L. major</em> was determined by quantifying parasite burdens in <em>L. major</em> infected footpads using a limiting dilution assay and by measuring lesion sizes of the infected footpad compared to the contralateral uninfected footpad.</p></div><div><h3>Results</h3><p>Mice receiving SLA plus artemisinin produced significantly high levels of IL-4 and IL-5 (<em>P</em> &lt; 0.05) and low levels of IFN-γ, resulting in exacerbated disease. In addition, subcutaneous administration of SLA + artemisinin, artemisinin alone or SLA alone resulted in the development of large footpad swellings and high parasite loads that were comparable to those of the control unvaccinated mice (<em>P</em> &gt; 0.05), resulting in exacerbated disease.</p></div><div><h3>Conclusion</h3><p>These data suggest that artemisinin is not a suitable adjuvant for <em>Leishmania</em> vaccines. However, since artemisinin has been shown to be effective against <em>Leishmania</em> parasites <em>in vitro</em> and <em>in vivo</em>, further studies ought to be conducted to determine its immunochemotherapeutic potential when co-administered with <em>Leishmania</em> antigens.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60084-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73501238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maxillary first premolars: I. Morphology of the apical constriction","authors":"Daming Wu,&nbsp;Younong Wu,&nbsp;Ming Hu","doi":"10.1016/S1007-4376(09)60093-9","DOIUrl":"10.1016/S1007-4376(09)60093-9","url":null,"abstract":"<div><h3>Objective</h3><p>To investigated the apical constriction morphology of maxillary first premolars in the Chinese population.</p></div><div><h3>Methods</h3><p>Eighty recently extracted human maxillary first premolars from a native Chinese population were used. The number and shape of apical constrictions were recorded under a dental operating microscope (DOM) at 12.5 × 2.5 magnification. After access preparation, a new K-file was inserted into the canal until the tip of the file was just seen at the apical constriction under the DOM. The teeth with files in the canals were X-rayed from a mesiodistal direction using a direct digital radiography (DDR) system, and the distance between the file tip and the center of radiographic apex was directly measured from the computer screen using DDR measurement software.</p></div><div><h3>Results</h3><p>The percentage of teeth with an apical constriction was 78.5% (102/130). The most common apical constriction shapes were oval (55.9%) and round (35.3%). The mean distance between the apical constriction and the anatomical tip of the root was 0.61 mm, and 84.3% (86/102) were within 1 mm.</p></div><div><h3>Conclusion</h3><p>The most common shape of an apical constriction was oval or round, and the distance to the apex was mostly within 1 mm, indicating that root canal therapy should stop 1 mm from the radiographic apex.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60093-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80169207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of gefitinib as a first-line single agent treatment in patients with advanced non-small cell lung cancer","authors":"Yongmei Yin ,&nbsp;Yiting Geng ,&nbsp;Xiaodong Li,&nbsp;Xiaoli Hu,&nbsp;Xiaofeng Chen,&nbsp;Wei Li,&nbsp;Yongqian Shu","doi":"10.1016/S1007-4376(09)60088-5","DOIUrl":"10.1016/S1007-4376(09)60088-5","url":null,"abstract":"<div><h3>Objective</h3><p>To assess the efficacy and toxicity of gefitinib as a single agent treatment in Chinese patients with advanced non-small cell lung cancer (NSCLC).</p></div><div><h3>Methods</h3><p>Forty-five patients with advanced NSCLC were treated with gefitinib at 250 mg daily until the disease progressed or the patient could not tolerate the toxicity.</p></div><div><h3>Results</h3><p>None of the patients achieved a complete response (CR), while 15 patients achieved a partial remission (PR) and 17 experienced a stable disease (SD). Thirteen patients continued to have a progressive disease (PD). The response rate and the disease control rate were 33.3% and 71.1%, respectively. The symptom remission rate was 72.5%, and the median remission time was 8 days. The median survival time was 15.3 months. The median progression-free survival time was 6.0 months. The most common toxicities included rash (53.3%) and diarrhea (33.3%). Dehydration and pruritus of the skin developed in 26.7% and 22.2% of the patients, respectively. Hepatic toxicity occurred in 6.7% of patients and oral ulceration occurred in 4.4% of patients.</p></div><div><h3>Conclusion</h3><p>Single agent treatment with gefitinib is effective against advanced NSCLC, and is well tolerated in Chinese patients.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60088-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89056954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}