Journal of Nanjing Medical University最新文献

{"title":"Neural histamine in the tuberomammillary nucleus regulates the onset of neurogenic pulmonary edema in rabbits","authors":"Rong Dong,&nbsp;Xiaohong Zhang,&nbsp;Lijuan Shi","doi":"10.1016/S1007-4376(09)60029-0","DOIUrl":"10.1016/S1007-4376(09)60029-0","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the effect of neural histamine in the tuberomammillary nucleus(TM) on neurogenic pulmonary edema (NPE) onset in rabbits and the function of the rostral ventrolateral medulla(RVLM) in the neural histamine modulation of NPE.</p></div><div><h3>Methods</h3><p>NPE was produced by the intracisternal injections of fibrinogen and thrombin. The contents of histamine in the TM and RVLM in rabbits were measured with high performance liquid chromatography(HPLC). Rabbits were placed on a stereotaxic frame and microinjection cannulae were inserted into the TM and RVLM using brain atlas coordinates. Animals were pretreated with R-α-methylhistamine(MeHA) in the TM and chlorphenamine Mmaleate/cimetidine in the RVLM prior to establishing the NPE model. Changes in the lung water ratio and mean arterial pressure(MAP) were recorded, and paraffin sections of lung tissue were observed by light microscope.</p></div><div><h3>Results</h3><p>We found that the contents of histamine(HA) in the TM and RVLM increased significantly with the onset of NPE. Pretreatment with MeHA in the TM and chlorphenamine Mmaleate in the RVLM significantly decreased MAP, and the lung water ratio and histological characteristics of the NPE in the rabbit model. Pretreatment with cimetidine in the RVLM had no effect on NPE.</p></div><div><h3>Conculsion</h3><p>The results suggest that neural histamine in the TM is involved in the onset of NPE, and this effect of neural histamine is mediated by H₁ receptor in the RVLM.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 1","pages":"Pages 64-68"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60029-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88741300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nursing of advanced colorectal cancer patients treated with Cetuximab combined with chemotherapy","authors":"Xiaoping Zhu,&nbsp;Chunli Wu","doi":"10.1016/S1007-4376(09)60016-2","DOIUrl":"10.1016/S1007-4376(09)60016-2","url":null,"abstract":"<div><p>Cetuximab is a new medication that has recently been approved for the treatment of advanced colorectal cancer. To date we have had little experience in using this targeted agent. Eleven patients in our hospital with advanced colorectal cancer were treated with cetuximab and chemotherapy. Based on the curative effect of this combination therapy, we have concluded that the following nursing practices make an important contribution to the patients'prognosis and wellbeing: to establish a good nurse-patient relationship, to increase patient understanding of the side effects, to standardize the medications, to observe and to deal with the side effects of the medications(for example skin reaction, neutropenia, and diarrhea), and to provide continuous mental health care support and education.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 398-400"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60016-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80331644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of metabotropic glutamate receptors mGluR5 and glutamate transporter EAAC1 in the myenteric plexus of the diabetic rat ileum","authors":"Yanhua Bai,&nbsp;Jun Zhang,&nbsp;Hongyang Shi,&nbsp;Fei Dai","doi":"10.1016/S1007-4376(09)60006-X","DOIUrl":"10.1016/S1007-4376(09)60006-X","url":null,"abstract":"<div><h3>Objective</h3><p>To study the morphologic abnormalities of the myenteric plexus in diabetic rats and to explore the mechanism of their effect on gastrointestinal motility.</p></div><div><h3>Methods</h3><p>Forty rats were randomly divided into a diabetic group and a control group, Gastric emptying and small intestine transit rates were measured and histologic and molecular changes in glutamatergic nerves in the ileal myenteric plexus were observed, mGluR5 receptor and EAAC1 transporter changes in the diabetic rats were studied using fluorescence immunohistochemistry and RT-PCR.</p></div><div><h3>Results</h3><p>Eighteen weeks after the establishment of the diabetic rats model, gastric emptying and small intestine transit rates were found to be significantly delayed in the diabetic group when compared with the control group. The density of glutamatergic ganglia and neurons in the ileal myenterie plexus were significantly decreased in the diabetic group when compared with control group(<em>P</em> &lt; 0.05) and the mGluR5 receptors and EAAC1 transporters were downregulated in the diabetic rats(<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>Decreased glutamatergic enteric ganglia and neurons and decreased mGluR5 receptors and EAAC1 transporters in the intestinal myenteric plexus is one of the mechanisms of diabetic gastroenteropathy in rats.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 356-361"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60006-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89655895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study on the Factors influencing insulin resistance in obese adolescents","authors":"Yongmei Jin ,&nbsp;Pengfei Dou","doi":"10.1016/S1007-4376(09)60014-9","DOIUrl":"10.1016/S1007-4376(09)60014-9","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the factors influencing insulin resistance in obese Chinese children.</p></div><div><h3>Methods</h3><p>We randomly selected 53 children with uncomplicated obesity between 9 to14 years of age, and 29 normal healthy children, matched for age and sex. Anthropometric and plasma biochemical variables(including lipid profiles, glucose and insulin) were measured using standard methods. We calculated insulin resistance(IR) index using homeostasis model assessment(HOMA) methods and measured plasma high-sensitivity C-reactive protein(hs-CRP) levels using nephelometric methods. All statistical analyses were conducted using the statistical package SPSS.</p></div><div><h3>Results</h3><p>Levels of fasting serum insulin, hs-CRP, total cholesterol(TC), low density lipoproteins cholesterol(LDL-C) and IR index were higher in obese children than in controls, while high-density lipoprotein cholesterol(HDL-C) values were lower in the obese children. There was no significant difference in levels of fasting blood glucose between the two groups. HOMA-IR was used as the dependent variable in multivariate regression analysis. Significant independent predictors for insulin resistance adjusted for waist/hip ratio, diastolic pressure (DBP), BMI, triglycerides and HDL-C level were waist circumference(WC), weight and systolic pressure(SBP).</p></div><div><h3>Conclusion</h3><p>Waist circumference, weight and SBP are predictors of insulin resistance syndrome in Chinese adolescents</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 391-394"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60014-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79252207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study of natural hydroxyapatite/chitosan composite on reconstructing bone defects","authors":"Hua Yuan ,&nbsp;Ning Chen ,&nbsp;Xiaoying Lü ,&nbsp;Buzhong Zheng","doi":"10.1016/S1007-4376(09)60009-5","DOIUrl":"10.1016/S1007-4376(09)60009-5","url":null,"abstract":"<div><h3>Objective</h3><p>To study the possibility of natural hydroxyapatite/chitosan composite on repairing bone defects.</p></div><div><h3>Methods</h3><p>We developed a natural hydroxyapatite/chitosan composite that could be molded into any desired shape. The powder component consists of natural hydroxyapatite, which is epurated from bone of pigs. The liquid component consists of malic acid and chitosan. Operations were performed on the left tibias of 15 white rabbits to create two square bone defects. One of the defects was reconstructed with the composite, while the other was not repaired and used as a blank control. Three of the animals were killed at the end of 2 weeks, 4 weeks, 8 weeks, 12 weeks and 16 weeks respectively and implants were evaluated anatomically and histologically.</p></div><div><h3>Results</h3><p>No apparent rejection reaction was found, except for a mild inflammatory infiltration observed 2 weeks after surgery. Fibrous tissue became thinner 2∼8 weeks after surgery and bony connections were detected 12 weeks after surgery. The new bone was the same as the recipient bone by the 16th postoperative week.</p></div><div><h3>Conclusion</h3><p>The hydroxyapatite/chitosan composite has good biocompatibility and osteoconduction. It is a potential repairing material for clinical application.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 372-375"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60009-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91258366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of Apparent Diffusion Coefficient(ADC) of Diffusion eighted Magnetic Resonance Imaging in Common Renal Disease Diagnosis","authors":"Yuelang Zhang,&nbsp;Xingwang Sun,&nbsp;Guangnan Quan,&nbsp;Yongqian Qiang,&nbsp;Chenxia Li","doi":"10.1016/S1007-4376(09)60007-1","DOIUrl":"10.1016/S1007-4376(09)60007-1","url":null,"abstract":"<div><h3>Objective</h3><p>To find the value of the apparent diffusion coefficient (ADC) of diffusion weighted magnetic resonance imaging of common renal diseases.</p></div><div><h3>Methods</h3><p>There were 30 healthy subjects and 81 patients with renal lesions (56 cases of renal carcinoma, 18 lesions of 12 cases of renal angiomyolipoma, and 21 lesions of 13 cases of renal cysts). Conventional magnetic resonance imaging and diffusion weighted magnetic resonance imaging were carried out. We measured the average ADC value of the renal lesions and normal kidneys. ADC maps from different b values were generated by a statistical package.</p></div><div><h3>Results</h3><p>The ADC values of normal kidneys with three different motion-probing gradients(b=500, 800, 1000 sec/mm²) were 2.78 ± 0.14, × 10⁻³mm²s⁻¹, 2.45 ± 0.13, × 10⁻³mm²s⁻¹, 2.13 ± 0.14, × 10⁻³mm²s⁻¹, respectively. The ADC values of renal cell carcinoma with three different motion-probing gradients(b=500, 800, 1000 sec/mm²) were 1.63 ± 0.14 × 10⁻³mm²s⁻¹, 1.31± 0.18 × 10⁻³mm²s⁻¹, 1.07 ± 0.15, 10⁻³mm²s⁻¹, respectively. Among the renal cell carcinoma, the ADC value of clear cell type were 1.67 ± 0.09, × 10⁻³mm²s⁻¹, 1.36 ± 0.13, × 10⁻³mm²s⁻¹, 1.15 ± 0.14, × 10⁻³mm²s⁻¹, respectively; the ADC values of granular cell type were 1.59 ± 0.19, × 10⁻³mm²s⁻¹, 1.25 ± 0.22, × 10⁻³mm²s⁻¹, 0.97 ± 0.12, × 10⁻³mm²s⁻¹, respectively. The ADC values of renal angiomyolipoma with three different motion-probing gradients(b=500, 800, 1000 sec/mm²) were 0.88± 0.08, 10⁻³mm²s⁻¹, 0.63 ± 0.07, × 10⁻³mm²s⁻¹, 0.43 ± 0.04, × 10⁻³mm²s⁻¹, respectively. The ADC values of renal cystic lesions with three different motion-probing gradients(b=500, 800, 1000 sec/mm²) were 3.73 ± 0.18, × 10⁻³mm²s⁻¹, 3.44 ± 0.13, × 10⁻³mm²s⁻¹, 3.09 ± 0.21, × 10⁻³mm²s⁻¹, respectively. Statistically significant differences exists between the ADC values of normal kidney, renal carcinomas, renal angiomyolipomas and renal cysts when the b value is the same. Among the different cell types of renal carcinomas, the ADC value of granular cell carcinoma is lower than that of clear cell carcinomas.</p></div><div><h3>Conclusion</h3><p>It is of benefit in diagnosing and distinguishing between benign and malignant renal tumors to know the ADC values in diffusion weighted magnetic resonance imaging. Furthermore, these values help to know the in","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 362-365"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60007-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83917109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discrimination of mitochondrial DNA 10400 locus by SNP-operated on/off Switch","authors":"Mei Hong,&nbsp;Enben Su,&nbsp;Ziqing Chen,&nbsp;Xiaobing Ju,&nbsp;Qi Chen,&nbsp;Rong Zhou","doi":"10.1016/S1007-4376(09)60004-6","DOIUrl":"10.1016/S1007-4376(09)60004-6","url":null,"abstract":"<div><h3>Objective</h3><p>To apply reformed AS-PCR, which combined phosphorothioate-modified primers with exo⁺ polymerase, in single nucleotide polymorphism discrimination of mitochondrial DNA 10400 locus.</p></div><div><h3>Methods</h3><p>We used the mtDNA 10400 locus to design unmodified and 3′ phosphorothioate-modified allele-specific primers for PCR, which was performed using polymerases with and without 3′ exonuclease activities. The effects of these primers on primer-extension were evaluated by agarose gel electrophoresis.</p></div><div><h3>Results</h3><p>The unmodified primers were extended by both exo⁻ and exo⁺ polymerase irrespective of whether the primers were matched or mismatched with the templates. However, the 3′ phosphorothioate-modified primers with a terminal mismatch triggered an, “off-switch” of exo⁺ polymerase when compared to exo⁻polymerase.</p></div><div><h3>Conclusion</h3><p>The, “on/off” switch constituted by the combination of 3′ phosphorothioate-modified primers with exo⁺ polymerase is a cost-effective, high-throughput and reliable method for SNP typing, which will be of enormous application in association studies by single nucleotide polymorphism screening.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 346-350"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60004-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89983713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation of Xive implants 3-year after placement","authors":"Ruixia Wang,&nbsp;Hua Yuan,&nbsp;Ning Chen,&nbsp;Guoping Wang,&nbsp;Zhaoping Fang,&nbsp;Guoxing Zhou","doi":"10.1016/S1007-4376(09)60011-3","DOIUrl":"10.1016/S1007-4376(09)60011-3","url":null,"abstract":"<div><h3>Objective</h3><p>The main purpose of this retrospective study is to evaluate clinical outcomes of Xive implants 3-year after insertion.</p></div><div><h3>Methods</h3><p>219 Xive implants were placed in 139 patients and their clinical effects were evaluated using the Albrektsson Standard.</p></div><div><h3>Results</h3><p>The 3-year survival rate of the Xive implants is 97.26%(213/219). Eleven cases of immediate implants and 15 cases of implant insertion after maxillary sinus lifting all succeeded. The failure ratio is 6/219(2.74%) with failures resulting from excess bone loss in the implant region, peri-implant mucosal inflammation or continuous pain caused by some unknown reasons.</p></div><div><h3>Conclusion</h3><p>Xive implants are clinically effective for the restoration of missing teeth. Immediate implant and implant following maxillary sinus lifting were also successful.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 379-381"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60011-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76797946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparisons of different methods of anesthesia and analgesia on the levels of glycometabolism rate-limiting enzymes in erythrocytes and plasma glucose and stress hormones in patients undergoing esophagus surgery","authors":"Xiaokun Zhang ,&nbsp;Xiongxiong Pan ,&nbsp;Yinbin Pan ,&nbsp;Jie Sun ,&nbsp;Yanning Qian","doi":"10.1016/S1007-4376(09)60012-5","DOIUrl":"10.1016/S1007-4376(09)60012-5","url":null,"abstract":"<div><h3>Objective</h3><p>To compare the effects of different methods of anesthesia and analgesia on the activities of phosphofructokinase(PFK), glucose-6-phosphate dehydrogenase(G-6PD) and aldose reductase(AR) in erythrocytes and levels of plasma glucose and stress hormones in patients undergoing esophagus surgery.</p></div><div><h3>Methods</h3><p>Sixty-two patients scheduled for esophagus surgery were randomly divided into three groups: group I(<em>n</em> = 20) receiving only general anesthesia(GA) followed by intravenous patient controlled analgesia(PCA) with fentanyl 15μg/kg. The other two groups receiving both general anesthesia combined with thoracic epidural anesthesia (GEA) and either intravenous PCA with fentanyl 15μg/kg (group II, <em>n</em> = 21) or thoracic epidural analgesia(TEA) with 0.125% ropivacaine and 0.0002% fentanyl mixture(group III, <em>n</em> = 21) after the operation. Venous blood samples were collected for measurements of PFK, G-6PD and AR activities in erythrocytes and plasma glucose, cortisol, epinephrine and norepinephrine before induction (T₁), 60 min following the incision (T₂), 60 min(T₃) after operation, on the 1st(T₄) and 2nd postoperative day(T₅).</p></div><div><h3>Results</h3><p>The activities of PFK decreased(<em>P</em> &lt; 0.01 or <em>P</em> = 0.004) and the activities of G-6PD and AR increased(<em>P</em> &lt; 0. 01) in groups I and II on T₄ compared with those on T₁. Between the two groups, the activities of these enzymes in group II changed less than those of group I(<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05). These enzymes activities changed slightly in group III on T₄(<em>P</em> &gt; 0.05). There were significant differences between group III and the other two groups(<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05). The levels of plasma glucose increased significantly on T₂(<em>P</em> &lt; 0.01), reached peak values on T₄(<em>P</em> &lt; 0.01) and fell on T₅ in the three groups. Compared to those of groups I and II, the values of plasma glucose in group III were lower on T₄ and T₅(<em>P</em> &lt; 0.05 or <em>P</em> &lt; 0.01). The cortisol concentration in each group increased significantly at T₂(<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05), and remained elevated on T₅(<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05), while on T₂ and T₃ the cortisol levels of group'were higher than that of groups II and III (<em>P</em> &lt; 0.05). The levels of group III were lower than those of the other groups on T₄ and T₅(<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05). The levels of epinephrine and norepinephrine were also significantly higher in group I than those of the other two groups on T₂(<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05), and their levels in group I and II were higher than that of group","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 382-386"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60012-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85769419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TNF-alpha-induced metastasis gene changes in MCF-7 cells","authors":"Xiaofeng Chen,&nbsp;Yongqian Shu,&nbsp;Wei Li,&nbsp;Yongmei Yin","doi":"10.1016/S1007-4376(09)60008-3","DOIUrl":"10.1016/S1007-4376(09)60008-3","url":null,"abstract":"<div><h3>Objective</h3><p>Studies have shown that TNF-α secreted by tumor cells and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF-α contributes to metastasis.</p></div><div><h3>Methods</h3><p>MCF-7 cells were cultured and treated with low dose TNF-α (20 ng/ml). cDNA array analysis was applied to detect the metastasis related gene expressions.</p></div><div><h3>Results</h3><p>A total of 36 gene expressions were significantly regulated by TNF-α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-α were somewhat time dependent.</p></div><div><h3>Conclusion</h3><p>TNF-α can enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 366-371"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60008-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88365275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}