{"title":"tnf - α诱导的MCF-7细胞转移基因的变化","authors":"Xiaofeng Chen, Yongqian Shu, Wei Li, Yongmei Yin","doi":"10.1016/S1007-4376(09)60008-3","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Studies have shown that TNF-α secreted by tumor cells and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF-α contributes to metastasis.</p></div><div><h3>Methods</h3><p>MCF-7 cells were cultured and treated with low dose TNF-α (20 ng/ml). cDNA array analysis was applied to detect the metastasis related gene expressions.</p></div><div><h3>Results</h3><p>A total of 36 gene expressions were significantly regulated by TNF-α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-α were somewhat time dependent.</p></div><div><h3>Conclusion</h3><p>TNF-α can enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"22 6","pages":"Pages 366-371"},"PeriodicalIF":0.0000,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60008-3","citationCount":"5","resultStr":"{\"title\":\"TNF-alpha-induced metastasis gene changes in MCF-7 cells\",\"authors\":\"Xiaofeng Chen, Yongqian Shu, Wei Li, Yongmei Yin\",\"doi\":\"10.1016/S1007-4376(09)60008-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Studies have shown that TNF-α secreted by tumor cells and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF-α contributes to metastasis.</p></div><div><h3>Methods</h3><p>MCF-7 cells were cultured and treated with low dose TNF-α (20 ng/ml). cDNA array analysis was applied to detect the metastasis related gene expressions.</p></div><div><h3>Results</h3><p>A total of 36 gene expressions were significantly regulated by TNF-α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-α were somewhat time dependent.</p></div><div><h3>Conclusion</h3><p>TNF-α can enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.</p></div>\",\"PeriodicalId\":100807,\"journal\":{\"name\":\"Journal of Nanjing Medical University\",\"volume\":\"22 6\",\"pages\":\"Pages 366-371\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60008-3\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nanjing Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1007437609600083\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nanjing Medical University","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1007437609600083","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
TNF-alpha-induced metastasis gene changes in MCF-7 cells
Objective
Studies have shown that TNF-α secreted by tumor cells and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF-α contributes to metastasis.
Methods
MCF-7 cells were cultured and treated with low dose TNF-α (20 ng/ml). cDNA array analysis was applied to detect the metastasis related gene expressions.
Results
A total of 36 gene expressions were significantly regulated by TNF-α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-α were somewhat time dependent.
Conclusion
TNF-α can enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.
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