Journal of Holistic Integrative Pharmacy最新文献

{"title":"Evolution of Salvia key enzyme genes based on metabolomic and transcriptomic insights into mitochondrial quality control","authors":"Zhenyu Zhao ,&nbsp;Huijie Li ,&nbsp;Boyao Wang ,&nbsp;Xuhao Gong ,&nbsp;Jinhua Gu","doi":"10.1016/j.jhip.2024.04.004","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.004","url":null,"abstract":"<div><p>The purpose of this study was to explore the relationship between the evolution of key <em>Salvia</em> enzyme genes and metabolite biological activity on mitochondrial quality control. Metabolomics and transcriptomics were performed to detect the metabolites of <em>Salvia</em> and <em>76AH1</em> (CYP450) genes and a maximum likelihood tree for <em>Salvia</em> was established. Additionally, the protein properties of 76AH were analyzed and the metabolite, as well as mitochondrial quality control, targets were downloaded from the TCMSP, BATMAN, and GeneCards databases and analyzed. Molecular docking of PINK1 with cryptotanshinone and tanshinone IIA was assessed and a molecular binding area was identified. Moreover, the specific types of <em>Salvia</em> secondary metabolites were accurately identified and quantified with nearly all AH1 genes having been sequenced. This study begins to elucidate the relationship between species evolution and biological activity. Specifically, the structural analysis demonstrates that cryptotanshinone and tanshinone IIA exhibit strong pharmacological activity in mitochondrial quality control.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 2","pages":"Pages 63-76"},"PeriodicalIF":0.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000190/pdfft?md5=941f77026b92e83fdd60a9f1ebe9230a&pid=1-s2.0-S2707368824000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141163796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity analysis of Cinnamomum cassia Presl and Cinnamomum cassia Presl var. macrophyllum Chu based on ISSR","authors":"Ziqi Zheng ,&nbsp;Danyun Xu ,&nbsp;Quan Yang ,&nbsp;Hongyang Gao","doi":"10.1016/j.jhip.2024.04.002","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.002","url":null,"abstract":"<div><p>ISSR technology can provide detailed data on the genetic information of species. In this study, ISSR was used to find dominant bands at the DNA level to stably identify <em>Cinnamomum cassia</em> Presl and <em>Cinnamomum cassia</em> Presl var. <em>macrophyllum</em> Chu, and <em>Cinnamomum cassia</em> Presl from different production areas. The results showed that primer UBC834 had dominant bands for <em>Cinnamomum cassia</em> Presl and <em>Cinnamomum cassia</em> Presl var. <em>macrophyllum</em> Chu samples, and primer UBC876 had dominant bands for <em>Cinnamomum cassia</em> Presl samples from different production areas. By analyzing the data of ISSR, it was found that the clustering results and the results of principal component analysis were consistent with the results of electropherograms and were divided into five categories. At the same time, the polymorphism analysis showed that <em>Cinnamomum cassia</em> Presl germplasm resources were rich in genetic diversity. In addition, the results of genetic clustering analysis showed that when the GS was higher than 0.88, it was possible to distinguish <em>Cinnamomum cassia</em> Presl and <em>Cinnamomum cassia</em> Presl var. <em>macrophyllum</em> Chu. As the genetic similarity coefficient increased, <em>Cinnamomum cassia</em> Presl from different production areas could be distinguished. The results of this study provide a scientific basis for the development and utilization of the germplasm resources of <em>Cinnamomum cassia</em> Presl and <em>Cinnamomum cassia</em> Presl var. <em>macrophyllum</em> Chu, which can meet the economic and ecological needs. It also provides a new perspective for the conservation of biodiversity and biological evolution.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 19-26"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000177/pdfft?md5=6e2849a9989c9de1b539a19fcb087ea4&pid=1-s2.0-S2707368824000177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140643650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the comprehensive evaluation of TCM service capacity in 71 county-level TCM hospitals of Zhejiang Province in China","authors":"Xuehui Meng ,&nbsp;Xiaoyue Xiao ,&nbsp;Jason Li ,&nbsp;Tong Pei ,&nbsp;Xinhao Lin ,&nbsp;Dan Wu","doi":"10.1016/j.jhip.2024.04.005","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.005","url":null,"abstract":"<div><h3>Objectives</h3><p>This study constructed an evaluation index system of Traditional Chinese Medicine (TCM) service capacity of county-level TCM hospitals, investigated the status quo of TCM service capacity of county-level TCM hospitals in Zhejiang Province, aiming to provide suggestions and references for the high-quality development of TCM hospitals in China.</p></div><div><h3>Methods</h3><p>Delphi expert consultation method and analytic hierarchy process were used to construct the comprehensive evaluation index system and its weight. Field investigation method was used to conduct a cross-sectional survey of 71 county-level TCM hospitals in Zhejiang Province, to calculate the scores of each index, and to compare the service capacity of county-level TCM hospitals.</p></div><div><h3>Results</h3><p>The evaluation index system includes 6 first-level indicators, 15 ​second-level indicators and 42 third-level indicators. The average score of service capacity of 71 county-level TCM hospitals was 60.73 points, among which the highest score was 80.72 points, and the lowest score was only 33.52 points. There are great differences in TCM service capacity among hospitals.</p></div><div><h3>Conclusions</h3><p>We have come to the conclusions that the index system can evaluate the service capacity of TCM hospitals effectively. In addition, there are still problems in the development of county-level TCM hospitals in Zhejiang Province, such as insufficient service capacity, great gap of development between regions, and inadequate development of characteristic advantages of TCM. Thus, it is necessary to consolidate the development foundation, pay more attention to the balance of regional development, and improve the service capacity of county-level TCM hospitals.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 27-32"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000207/pdfft?md5=a693d508e93f1728208f28da78b94207&pid=1-s2.0-S2707368824000207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining metabolomics and 16S rRNA sequencing to investigate the effects of fecal microbiota transplantation intervention in aging mice","authors":"Tianxin Zhu ,&nbsp;Ling Yang ,&nbsp;Zhizhong Luo ,&nbsp;Jiqian He ,&nbsp;Xuefeng Xu ,&nbsp;Duosheng Luo","doi":"10.1016/j.jhip.2024.04.007","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.007","url":null,"abstract":"<div><p>Aging is a complex physiological process, accompanied by metabolic and immune disorders leading to aging. The aging process yields dramatic alterations in the gut microbiota. However, there is limited evidence for a mechanistic role of the gut microbiota in aging processes. We conducted fecal microbiota transplantation from either young (4 months) or aged (22 months) donor mice into aged recipient mice (22 months) or young (4 months). Aged mice receiving young donor gut microbiota reversed aging-associated inflammation in serum and issues, and the behavioral characteristics with spontaneous activities, standing times, the number of falls, and the latency time in aged mice were alleviated. Conversely, young mice receiving aged donor gut microbiota promoted the development of inflammation, and the behavioral characteristics of aging were intensified. The relative abundance of <em>Odoribacter</em>, <em>Flexispira</em>, <em>Veillonella,</em> and <em>Prevotellaceae_Prevotella</em> were significantly enriched in aged mice, while changes were reversed in aged mice receiving young donor gut microbiota. The metabolites including indole acrylic acid, LPC (18:1/0:0), LPC (15:0/0:0), LPC (0:0/15:0) were up-regulated, and xanthine, inosine, allopurinol, N6-methyladenosine metabolites were down-regulated. After aged mice receiving young donor gut microbiota, these metabolite changes were reversed too. Analysis of the correlation between the microbiota, metabolites, and inflammatory cytokines showed indoleacrylic acid, LPC (18:1/0:0), LPC (15:0/0:0), and LPC (0:0/15:0) were negatively associated with <em>Adlercreutzia</em>, while positively correlated with <em>Veillonella</em>, <em>Prevotellaceae_Prevotella</em>, <em>Streptococcus</em>, and <em>Odoribacter</em>. 1-methylinosine, L-methyladenosine, allopurinol, and N6-methyladenosine were positively correlated with <em>Desulfovibrio</em>, and negatively correlated with <em>Veillonella</em> and <em>Prevotellaceae_Prevotella</em>. Indoleacrylic acid was negatively correlated with TGF-β. LPC (18:1/0:0), LPC (15:0/0:0), and LPC (0:0/15:0) were positively correlated with IL-10. 1-methylinosine, L-methyladenosine, N6-methyladenosine and inosine were negatively correlated with IL-6 and LPS. Our findings suggest that modulating the gut microbiome may be an appropriate treatment to promote healthy aging.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 33-44"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000220/pdfft?md5=b20cf3378fced86d90c22cc5197cbec1&pid=1-s2.0-S2707368824000220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological underlying mechanisms of the anticancer effect of licorice: Bioinformatics and experimental verification","authors":"Mengqi Liu ,&nbsp;Lingping Fu ,&nbsp;Hao Fu ,&nbsp;Yu Chen ,&nbsp;Mengxia Wu ,&nbsp;Hanchun Liu","doi":"10.1016/j.jhip.2024.04.006","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.006","url":null,"abstract":"<div><h3>Purpose</h3><p>Cancer is the second leading cause of human mortality and has rapidly become a major global issue. According to traditional Chinese medicine (TCM) theory, the primary pathogenesis of cancer is the deficiency of \"vital Qi\". Licorice (Gancao in Chinese, GC) one of the oldest and the most frequently used herbs for tonifying Qi, is commonly integrated with other Chinese medicines to treat cancer in TCM clinical practice. GC extract, or its chemical components, are often utilized in cancer treatment. However, the molecular mechanisms underlying GC's therapeutic effects on cancer remain unclear. Our current study aimed to elucidate these mechanisms from the perspective of network pharmacology and experimental validation.</p></div><div><h3>Methods</h3><p>We employed network pharmacology and bioinformatics to explore the anti-cancer mechanism, crucial targets, and kernel ingredients of GC. The significant targets and key ingredients (quercetin, glycyrrhetinic acid, and glucyrin) were substantiated using the Cancer Genome Atlas (TCGA) and Cell Counting Kit-8 (CCK-8) viability assay, respectively.</p></div><div><h3>Results</h3><p>Our findings identified eight critical targets and key ingredients (quercetin, glycyrrhetinic acid, glucyrin) and suggested that GC's anti-cancer effects are primarily associated with modulation of the immune system, signal transduction, cell cycle, and gene expression. The efficacy of quercetin, glycyrrhetinic acid, glucyrin against Human A549 lung cancer cells was confirmed.</p></div><div><h3>Conclusion</h3><p>This study comprehensively elucidates the mechanisms through which GC addresses cancer, leveraging network pharmacology and experimental validation. These insights contribute to identifying principal compounds and developing innovative drugs for cancer therapy.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 45-55"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000219/pdfft?md5=3d1a92d2f359aeb92fc3bb5a4cde7208&pid=1-s2.0-S2707368824000219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140947423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic evaluation of isosorbide mononitrate sustained-release capsules for the treatment of angina pectoris","authors":"Yuhang Liu ,&nbsp;Jienan Zheng ,&nbsp;Yeyou Xu ,&nbsp;Shuli Zhang ,&nbsp;Yueyun Li ,&nbsp;Hui Zhang","doi":"10.1016/j.jhip.2024.04.003","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.003","url":null,"abstract":"<div><h3>Objective</h3><p>Systematically evaluate the efficacy of (1) isosorbide mononitrate sustained-release capsules combined with conventional therapy and (2) conventional therapy alone in the treatment of angina pectoris and analyze their economic value to provide evidence and a reference for clinical medication and decision-making.</p></div><div><h3>Methods</h3><p>Randomized controlled trials on the efficacy of isosorbide mononitrate sustained-release capsules combined with conventional therapy and conventional therapy in the treatment of angina pectoris were searched and abstracted from major Chinese and English literature databases from August 26, 2003 to August 26, 2023, to calculate the rate of improvement in clinical symptoms using isosorbide mononitrate sustained-release capsules combined with conventional therapy (observation group) and using conventional therapy alone (control group). Review Manager 5.4 software was used for meta-analysis, and an economic evaluation was performed using cost-effectiveness analysis (CEA).</p></div><div><h3>Results</h3><p>The effectiveness rates for clinical symptom improvement were 95% in the observation group and 78% in the control group. According to the meta-analysis results, the efficiency of clinical symptom improvement in the observation group, which received the combination therapy with isosorbide mononitrate sustained-release capsules, was significantly better than that of the control group (OR ​= ​3.38, 95% CI: 2.28 to 4.99, <em>P</em> ​&lt; ​0.001). The CEA indicated an incremental cost-effectiveness ratio of 434.12 yuan, and the sensitivity analysis results demonstrated that the evaluation outcomes were stable, suggesting a robust foundation for the study's conclusions.</p></div><div><h3>Conclusions</h3><p>Compared with conventional therapy alone, isosorbide mononitrate sustained-release capsules combined with traditional treatment have good efficacy and safety in the treatment of angina pectoris and have certain economic advantages.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 56-62"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000189/pdfft?md5=bdf6519fe9293a110c942eacc49acc18&pid=1-s2.0-S2707368824000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140952202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence","authors":"Yutong Zhang ,&nbsp;Meixia Xie ,&nbsp;Jiayin Liang ,&nbsp;Li Li ,&nbsp;Shumei Wang ,&nbsp;Minghua Xian","doi":"10.1016/j.jhip.2024.04.001","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.04.001","url":null,"abstract":"<div><h3>Objective</h3><p>Previous studies have shown that Danhong injection (DHI) has a protective effect on the blood-brain barrier (BBB) function after ischemic stroke. However, the role of DHI in protecting the integrity of the BBB after ischemic stroke through endothelial cells is still unclear. The purpose of this study is to explore the role and mechanism of DHI in protecting BBB by interfering with endothelial cells.</p></div><div><h3>Methods</h3><p>We used network pharmacology technology to determine the potential pathways and mechanisms of DHI in treating ischemic stroke through endothelial cells. On account of the network pharmacology results, we assessed the effects of DHI in oxygen-glucose deprivation (OGD) model of mouse brain-derived endothelial (bEnd.3) cells via MTT, and validated the molecular mechanisms of DHI improving BBB injury through Western blot.</p></div><div><h3>Results</h3><p>Network pharmacology analysis suggested that DHI may regulate the PI3K/AKT signaling pathway of endothelial cells in the treatment of ischemic stroke. Cellular experiments showed that DHI stimulates endothelial cell migration and reduces OGD-induced BBB damage via VEGFR2/PI3K/AKT pathway.</p></div><div><h3>Conclusion</h3><p>Network pharmacology analysis and cellular experiments have shown that DHI alleviated the BBB damage by activating the VEGFR2/PI3K/AKT signaling pathway on endothelial cells.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 10-18"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000165/pdfft?md5=acdc42b921cd0790171af8d8ea952319&pid=1-s2.0-S2707368824000165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140622257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Analysis of clinical drug use and construction of pharmacy service model for children with Kawasaki disease based on integration concept” [J Holist Integr Pharm (2023) 133–139]","authors":"Huashen He ,&nbsp;Yingyao Luo ,&nbsp;Binghong Yu ,&nbsp;Yingqiang Lai ,&nbsp;Jinkun Zheng ,&nbsp;Junfeng Ban","doi":"10.1016/j.jhip.2024.03.001","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.03.001","url":null,"abstract":"","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Page 1"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000141/pdfft?md5=362f95fa5f20ee7f1e6e361de8152970&pid=1-s2.0-S2707368824000141-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140343732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacognostic studies on three species of Spermacoce","authors":"Shan Fan,&nbsp;Wenfeng Weng,&nbsp;Shengguo Ji","doi":"10.1016/j.jhip.2024.03.002","DOIUrl":"https://doi.org/10.1016/j.jhip.2024.03.002","url":null,"abstract":"<div><p>To provide a scientific basis for identification of <em>Spermacoce</em> species, including <em>Spermacoce remota</em> Lamarck, <em>Spermacoce exilis</em> (L. O. Williams) C. D. Adams, and <em>Spermacoce alata</em> Aubl., the current study was carried out for pharmacognostical parameters. Roots, stems, and leaves of plants were collected for pharmacognostical studies involving source identification, microscopic evaluation, character observation, phytochemical screening, ultraviolet spectrum and molecular pharmacognosy analysis. <em>S. remota</em>, <em>S. alata</em>, and <em>S. exilis</em> belong to the same genus, and the morphology of the plants was similar. Powder microscopy showed the presence of fibers, needle crystal bundles, spiral vessels, and near spherical pollen grains in all the plants. Phytochemical investigation demonstrated that <em>S. remota</em>, <em>S. alata</em>, and <em>S. exilis</em> all contained glycosides, proteins and volatile oils. In addition, the extracts of <em>S. alata</em> and <em>S. exilis</em> revealed the presence of flavonoids and tannins. In this study, the ITS sequence of <em>S. exilis</em> was found for the first time which had been submitted to NCBI to obtain the GenBank registration number. The results of neighbor-joining phylogenetic tree showed that <em>S. exilis</em>, <em>S. remota</em> and <em>S. alata</em> could be distinguished accurately. The study will be beneficial to the identification, resource conservation and quality control of <em>Spermacoce</em> species.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"5 1","pages":"Pages 2-9"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368824000153/pdfft?md5=d847d7f26f0deb88024f245cd579201b&pid=1-s2.0-S2707368824000153-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140345107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining single-cell RNA sequencing data and network pharmacology to explore the mechanism of action of Dayuan Yin in the treatment of acute lung injury","authors":"Lei Zhang,&nbsp;Wei Zhu,&nbsp;Zepeng Zhang,&nbsp;Yu Huang","doi":"10.1016/j.jhip.2024.01.002","DOIUrl":"10.1016/j.jhip.2024.01.002","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to analyze the usefulness of combined single-cell RNA sequencing data and network pharmacology in understanding the molecular regulation mechanism of Dayuan Yin (DYY) in acute lung injury (ALI).</div></div><div><h3>Methods</h3><div>The single-cell ALI dataset GSE224938 was acquired from the Gene Expression Database and cellular heterogeneity was examined using the Seurat software package. Differential expression analysis was conducted using the R software to identify genes with significant expression differences. The active constituents and therapeutic targets of DYY were acquired from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Subsequently, the protein-protein interaction (PPI) networks, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to analyze the key targets. A visual network depicting the interaction between compounds, targets, and pathways was constructed, and the CytoNCA plug-in was utilized to identify core targets. The active component-core target relationship was validated using MOE, AutoDockTools, AutoDockVina, and other software. Finally, a preliminary experiment was conducted using the lipopolysaccharide-induced acute lung injury (ALI) rat model.</div></div><div><h3>Results</h3><div>In total, 5243 significantly differentially expressed genes were identified in ALI, and 260 genes were identified as DYY targets. Then, 81 target genes were identified at the intersection between drugs and diseases. The identified core target genes included PIK3R1, IL-1β, IL-6, ICAM1, and CCL2. GO analysis was mainly involved in cellular inflammatory response, dual regulation of cell apoptosis, and coordinated migration. KEGG analysis showed enrichment in inflammatory pathways. The major active components were well connected with IL-1β. DYY could significantly reduce the phosphorylation expression of PI3K, Akt, and NF-κBp65 in the lung tissue of ALI rats, and regulated the activation of related inflammatory cells.</div></div><div><h3>Conclusions</h3><div>We successfully screened the potential active ingredients of DYY for the treatment of ALI. In addition, in vivo experiments preliminarily verified the predictions of network pharmacology, showing that DYY can inhibit the PI3K/Akt/NF−КВ signaling pathway, reduce cytokine release and regulate the number of inflammatory cells, providing an alternative for ALI treatment.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 4","pages":"Pages 259-271"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143291708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}