Journal of Holistic Integrative Pharmacy最新文献

{"title":"Dioscoreae persimilis polysaccharide ameliorates DSS-induced ulcerative colitis in mice through modulation of microbiota composition","authors":"Qian Zhang ,&nbsp;Guorong Wu ,&nbsp;Shumin Shen ,&nbsp;Chong Li","doi":"10.1016/j.jhip.2023.09.004","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.09.004","url":null,"abstract":"<div><p>Ulcerative colitis (UC) is a non-specific inflammatory bowel disease that has a high rate of recurrence, development of novel therapeutic approaches with high efficacy and few adverse effects are still needed. <em>Dioscoreae persimilis</em> is an edible plant that has been widely consumed as a remedy for gastrointestinal diseases in traditional Chinese medicine. Polysaccharides have been proven to have protective effects on UC. However, the role of polysaccharides from <em>D. persimilis</em> in UC has not been studied yet. The refined <em>D. persimilis</em> Polysaccharide (DP), which consists of glucose and galactose, was extracted and purified using three-phase partitioning (TPP) method. The primary chemical and structural characteristics of DP were investigated by UV, FT-IR, molecular weight, and monosaccharide composition. Based on dextran sulfate sodium (DSS) induced UC in mice, the alleviatory effect of DP on UC was explored. DP was found to alleviate histopathological changes of colon, improve colonic antioxidant capacity and ameliorate inflammation response in colitis mice. Moreover, 16S rDNA sequencing of fecal revealed that DP could restore the diversity and composition of gut microbiota, especially up-regulates the abundance of <em>Acetatifactor</em>, <em>Lachnospiraceae</em>, and <em>Lactobacillus</em>, and increase the ratio of Firmicutes/Bacteroidetes. According to this study, DP has the potential to serve as an effective nutritional supplement for improving colitis.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 2","pages":"Pages 157-165"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823000870/pdfft?md5=3449395a1f245bdf9f5916c92cf00ed6&pid=1-s2.0-S2707368823000870-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tianhuang formula ameliorates non-alcoholic fatty liver diseases in type 2 diabetic mice through CRLS1-ATF3/ChREBP pathway","authors":"Yi Han ,&nbsp;Yating Zhao ,&nbsp;Xuefeng Xu ,&nbsp;Zhizhong Luo ,&nbsp;Duosheng Luo ,&nbsp;Jiao Guo","doi":"10.1016/j.jhip.2023.09.002","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.09.002","url":null,"abstract":"<div><h3>Objective</h3><p>Tianhuang Formula (THF) is a hospital formula summarized by Professor Jiao Guo's 30 years of clinical experience. Some studies have shown that it can alleviate dyslipidemia in the body. The purpose of this study is to confirm whether THF can improve non-alcoholic fatty liver diseases (NAFLD) in type 2 diabetic mice induced by high-fat diet (HFD)/streptomycin (STZ) and to clarify its potential mechanism.</p></div><div><h3>Methods</h3><p>After induction of diabetes, mice were administrated with THF (60 ​mg/kg or 120 ​mg/kg) once daily for 10 weeks. Blood glucose (FBG), glucose tolerance, and insulin resistance (IR) were assayed by oral glucose tolerance test (OGTT) and insulin tolerance test (ITT). Blood lipids, alanine transaminase (ALT), and aspartate transaminases (AST) were detected. Serum fasting insulin (INS) and adiponectin (APN) levels were measured using ELISA. Histological changes in liver and pancreatic islets were observed by H&amp;E staining, followed by Oil Red O staining for liver lipid quantification and periodic acid-Schiff (PAS) staining to detect glycogen accumulation. Western blotting detected the levels of fatty cardiolipin synthase 1 (CRLS1), transcription factor activator 3 (ATF3), and carbohydrate-responsive element binding protein (ChREBP) in the liver. The mRNA transcripts of hepatic inflammatory factors, lipogenesis and lipolysis-related genes, and gluconeogenic enzyme-phosphoenolpyruvate carboxykinase (PEPCK), CRLS1, ATF3, and ChREBP mRNA levels were evaluated by RT-qPCR.</p></div><div><h3>Results</h3><p>THF restored impaired glucose tolerance and insulin resistance, respectively. There was an improvement in HFD/STZ-induced liver and islet damage, high serum HDL-C and ANP levels, and a significant decrease in FBG, total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), FFA, INS, ALT, and AST, and lipid droplet counts in the T2DM mice treated with THF. CREBBP binding protein ATF3 mediated the insulin resistance signaling pathway, which regulated glucose and lipid metabolism in the liver. THF upregulated CRLS1, and ChREBP downregulated the expression of downstream ATF3 in the liver. RT-qPCR analysis also systemically indicated that THF suppressed the pathway and key regulators related to inflammation, lipid accumulation, and gluconeogenesis.</p></div><div><h3>Conclusion</h3><p>Our findings demonstrated that THF ameliorated lipid profile and attenuated liver steatosis in T2DM mice through CRLS1-ATF3/ChREBP pathway activation.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 2","pages":"Pages 147-156"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823000857/pdfft?md5=7d230b18bbe01d2bb10e3b948adee93b&pid=1-s2.0-S2707368823000857-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of compounds and activities from mangrove Sonneratia genus and their endophytes","authors":"Bin Liu ,&nbsp;Xin Wang ,&nbsp;Yiming Wang ,&nbsp;Xiaohong Chen ,&nbsp;Xiaobao Jin ,&nbsp;Xiongming Luo","doi":"10.1016/j.jhip.2023.11.003","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.11.003","url":null,"abstract":"<div><p><em>Sonneratia</em> is an important mangrove plant, and its fruit has been used as traditional medicine and food in southeast China. With recent research focusing on its compounds and activities, an increasing number of compounds with novel structures and excellent antitumor, antioxidant, and other activities have been discovered. This review covered the compounds and activities of six species of the genus <em>Sonneratia</em> and their endophytes. To date, 116 compounds of <em>Sonneratia</em> have been reported, including 26 terpenoids, 9 flavonoids, 17 phenols, 9 lignans, 27 acid lipids, 16 steroids, and 12 other compounds. The main activities of the compounds in <em>Sonneratia</em> are antioxidant, antitumor, liver protection, antibacterial, and antidiabetic. Research on the compounds of endophytes from <em>Sonneratia</em> was first reported in 2009, and 56 compounds have been isolated, which mainly include sesquiterpenes, peptides, phenanthropyran ring-structured acids, pyrones, and anthracene derivatives. Individual compounds have been produced in <em>Sonneratia</em> and their endophytes that have the same structural fragments, and their interactions with small molecules and sources require further study. Recent advances in the bioactivities and compounds in the <em>Sonneratia</em> genus and their endophytes were summarized in this review, which is useful for the future isolation and discovery of active compounds and research regarding chemical ecology from the perspective of secondary metabolites.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 3","pages":"Pages 218-227"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823001085/pdfft?md5=0b29509c069669067fa3d11b3ac759ad&pid=1-s2.0-S2707368823001085-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138501085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on Prunella vulgaris and its monomers in protecting against ulcerative colitis","authors":"Fu Jinyin ,&nbsp;Yuan Yue ,&nbsp;Li Xiaojia ,&nbsp;Lin Peng ,&nbsp;Wang Shuibin ,&nbsp;Xiao Mingzhu","doi":"10.1016/j.jhip.2023.11.002","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.11.002","url":null,"abstract":"<div><p><em>Prunella vulgaris</em> is a traditional Chinese herbal medicine with many pharmacological effects, among which the anti-inflammatory effect is more significant. It is widely reported that <em>Prunella vulgaris</em> has anti-inflammatory, antioxidant, immune regulation, intestinal flora regulation and intestinal barrier protection effects on ulcerative colitis (UC). This paper collected relevant reports to further summarize the mechanisms and effective parts of <em>Prunella vulgaris</em> and its monomers in the treatment of UC and provided theoretical basis and reference for the application of <em>Prunella vulgaris</em> in UC.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 3","pages":"Pages 210-217"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823001073/pdfft?md5=4fb77f5a09d6dfca127da37aeb8f1229&pid=1-s2.0-S2707368823001073-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138480486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forsythiae Fructus attenuates cisplatin-induced cytotoxicity in IEC-6 ​cells and J774A.1 macrophages by inhibiting NLRP3/caspase-1/GSDMD mediated pyroptosis","authors":"Binbin Ye ,&nbsp;Ruifang Zhang ,&nbsp;Yihong Xian,&nbsp;Xiuxiu Liao,&nbsp;Weijian Chen,&nbsp;Ke Nie","doi":"10.1016/j.jhip.2023.09.001","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.09.001","url":null,"abstract":"<div><h3>Objective</h3><p>Forsythiae Fructus (lian qiao in Chinese), the dried fruit of <em>Forsythia suspensa</em> (Thunb.) Vahl, is a commonly used traditional Chinese medicine known for its diverse biological activities, including antiemetic, anti-inflammatory, antioxidant, antiviral, and neuroprotective properties. This study investigated the protective effects of Forsythiae Fructus and its primary components, phillyrin and forsythoside A, against cisplatin-induced cytotoxicity <em>in vitro</em>, specifically focusing on the intestinal epithelial cells (IEC-6) and the J774A.1 macrophage cell line.</p></div><div><h3>Methods</h3><p>Cisplatin and <em>tert</em>-butyl hydroperoxide (tBHP) were used to induce stress in IEC-6 ​cells, while cisplatin and lipopolysaccharides (LPS)/adenosine triphosphate (ATP) were employed for J774A.1 macrophages. The protective effects of Forsythiae Fructus aqueous extract (FAE), phillyrin, and forsythoside A against cytotoxicity in these cultured cells were evaluated. Cell viability was assessed using the Cell Counting Kit-8 assay, while cell membrane permeability was determined through Hoechst 33342 and propidium iodide staining. Intracellular reactive oxygen species (ROS) levels were investigated using DCFH-DA, and the expression of mRNA and protein related to the NLRP3 inflammasome and GSDMD-induced pyroptosis was quantified through qRT-PCR and western blotting.</p></div><div><h3>Results</h3><p>In IEC-6 ​cells, combining FAE, phillyrin, or forsythrin A with a subthreshold dose of the antioxidant N-acetyl-L-cysteine (NAC) significantly mitigated cisplatin- or tBHP-induced cell necrosis and restored impaired cell viability. Additionally, the upregulation of NF-<em>κ</em>B, ASC, NLRP3, caspase-1, GSDMD, and HMGB1 at both mRNA and protein levels induced by cisplatin or tBHP was markedly reversed with the joint intervention of FAE, phillyrin, or forsythrin A with NAC. Similarly, in cisplatin- or LPS/ATP-treated J774A.1 macrophages, the effects on cell necrosis, cell viability, and the NLRP3/caspase-1/GSDMD pathway mirrored our previous findings in IEC-6 ​cells.</p></div><div><h3>Conclusion</h3><p>The study suggests that the alleviating effect of Forsythiae Fructus and its primary components, phillyrin and forsythoside A, against cisplatin-induced cytotoxicity may be attributed to inhibiting oxidative stress, downregulating the NLRP3/caspase-1/GSDMD pathway, and inhibiting pyroptosis.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 2","pages":"Pages 166-177"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823000845/pdfft?md5=27785c23603c88792c15e18d89d414b9&pid=1-s2.0-S2707368823000845-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Ruthenium(II) complex based long lifetime phosphorescent probe for copper ions and pH detection","authors":"Yan Chen,&nbsp;Xufeng Mai,&nbsp;Jiecheng Zhao,&nbsp;Cuiqin Huang,&nbsp;Jun Li,&nbsp;Zhuopeng Ruan,&nbsp;Huijuan Yu","doi":"10.1016/j.jhip.2023.11.004","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.11.004","url":null,"abstract":"<div><h3>Objective</h3><p>Transition metal ruthenium(II) complex was used to prepare a dual-functional phosphorescent probe for the detection of Cu²⁺ ion and pH.</p></div><div><h3>Methods</h3><p>The ligand (E)-6-((thiazol-2-yl methylene)amino)-1,10-phenanthrolin-5-amine <strong>(tmpa)</strong> was prepared by condensation of 5,6-diamine-1, 10-phenanthroline with 2-formylthiazole, and then ruthenium(II) complex [Ru(bpy)₂tmpa](PF₆) (<strong>Ru-tmpa</strong>, bpy ​= ​2,2′-bipyridine) was synthesized by the coordination of <strong>tmpa</strong> with <em>cis</em>-Ru(bpy)₂Cl₂. Phosphorescence spectra were measured to investigate its response to metal ions (Li⁺, Na⁺, K⁺, Mg²⁺, Ca²⁺, Mn²⁺, Pb²⁺, Cr²⁺, Co²⁺, Ni²⁺, Cu⁺, Cu²⁺, Fe³⁺, Fe²⁺ and Zn²⁺). The binding affinity and detecting limit of <strong>Ru-tmpa</strong> to copper ions were tested by titration experiment. The ability of the probe to monitor copper ion levels inside cells was tested by confocal microscopy imaging.</p></div><div><h3>Results</h3><p><strong>Ru-tmpa</strong> can rapidly, sensitively and selectively detect Cu²⁺ in water and biological sample, and shows a sensitive phosphorescence response to pH. <strong>Ru-tmpa</strong> can penetrate cell membrane, enter the cell, and monitor the level of copper ions inside cell.</p></div><div><h3>Conclusion</h3><p>A novel fluorescent probe <strong>Ru-tmpa</strong> was synthesized to provide a powerful tool for the detection of Cu²⁺ and pH in biological and environmental systems.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 3","pages":"Pages 228-233"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823001097/pdfft?md5=70415bbe97fe71292b9943c164514ae3&pid=1-s2.0-S2707368823001097-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomics-based tools for drug discovery and development: From network maps to efficacy prediction","authors":"Junhao Fang ,&nbsp;Qi Chen ,&nbsp;Guoyu Wu","doi":"10.1016/j.jhip.2023.11.001","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.11.001","url":null,"abstract":"<div><p>Computing technology plays a crucial role in the field of drug discovery and development. With the rapid development of genomics and the improvement of databases, the application of genomics-based tools is important in drug discovery and development. These tools can deeply explore the information in gene expression profile databases, revealing the connections and interactions between drugs, diseases and genes, and providing strong support for drug discovery and development. This paper introduces various significant genomics-based tools for drug discovery and development, discusses the advantages of deep learning and artificial intelligence in utilizing large-scale genomic data, and reveals the development trends and future prospects of drug genomics tools. The continuous progress of these tools will provide more accurate and efficient support for drug discovery and development.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 3","pages":"Pages 199-209"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823001061/pdfft?md5=2fc78205240f5611dd640ec201df07aa&pid=1-s2.0-S2707368823001061-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138413162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacognostical study of Croton Crassifolius Geisel","authors":"Zhuojun Huang,&nbsp;Wenfeng Weng,&nbsp;Shengguo Ji","doi":"10.1016/j.jhip.2023.11.005","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.11.005","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Croton Crassifolius</em> Geisel of the genus <em>Croton</em> in the Euphorbia family is a widely distributed herb in South China. It has medicinal value for the treatment of a sore throat, soothing tendons, activating collateral, and treating rheumatoid arthritis. Although it has been traditionally used in Chinese medicine, there has been no systematic study on its identification and classification of the authenticity of this plant. The purpose of this study is to provide a scientific basis for the identification and classification of the authenticity of this plant.</p></div><div><h3>Methods</h3><p>An accurate and effective identification system was established through morphological characteristics, microscopic characteristics, physical and chemical parameter determination, phytochemical screening, and DNA barcoding analysis.</p></div><div><h3>Results</h3><p>The physicochemical results revealed that this plant might contain flavonoids, cardiac glycosides, and alkaloids. The ITS locus in the nuclear genome in the chloroplast genome was screened and evaluated and Neighbor-Joining phylogenetic trees can effectively identify <em>Croton Crassifolius</em> Geisel to a certain extent.</p></div><div><h3>Conclusion</h3><p>This research contributed to the development of species identification.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 3","pages":"Pages 234-240"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823001103/pdfft?md5=2b796a094f23ec576946f706295f514c&pid=1-s2.0-S2707368823001103-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination treatment of a deep diabetic toe ulcer with collagenase-santyl dressings and antibiotics: A case report","authors":"Salkapuram Sunil Kumar ,&nbsp;Karthikeyan Elumalai ,&nbsp;Srinivasan Sivannan ,&nbsp;Sivaneswari Srinivasan ,&nbsp;Santhana Krishnan Ramanujam ,&nbsp;Binoy Varghese Cherian","doi":"10.1016/j.jhip.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.11.006","url":null,"abstract":"<div><p>A diabetic foot ulcer is defined as the ulceration of tissues brought on by trauma and some peripheral neurological abnormalities, primarily as a result of the bacterial infection. The infection of the toe's surrounding tissue is the primary cause of the infectious diabetic toe ulcer. In this case report, a 58-year-old male patient with a deep foot ulcer was admitted to the emergency ward on October 15, 2022, due to pus discharge at the right big toe of the foot. Antibiotics like Vancomycin 30mg per kg twice per day and Ciprofloxacin 400mg twice per day were suggested, along with enzymatic debridement therapy, which helps stop infections faster. A decrease in wound size and an improvement in overall health showed that the patient's response to the combination therapy was encouraging. The use of collagenase-santyl dressings, along with suitable antibiotics, can play a crucial role in the successful treatment of foot ulcers by facilitating wound healing and preventing complications like cellulitis or osteomyelitis.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 3","pages":"Pages 256-258"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823001115/pdfft?md5=37e8fda96fece55656e6579e7efbaf63&pid=1-s2.0-S2707368823001115-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139099698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiaochaihu decoction induces Bel-7402/5-FU cell apoptosis and autophagy via PI3K/AKT/mTOR pathway","authors":"Xuejun Zhang ,&nbsp;Shilan Chen ,&nbsp;Xuejiao Wang ,&nbsp;Jiao Peng ,&nbsp;Jiumao Lin ,&nbsp;Jinyan Zhao","doi":"10.1016/j.jhip.2023.09.007","DOIUrl":"https://doi.org/10.1016/j.jhip.2023.09.007","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to observe the inhibitory effects of xiaochaihu decoction (XCHD) on human hepatocellular carcinoma (HCC) cells resistant to 5-fluorouracil (5-FU) (Bel-7402/5-FU) <em>in vitro</em> and <em>in vivo</em> and investigate its possible mechanisms.</p></div><div><h3>Methods</h3><p>Bel-7402 ​cells and their resistant cells to 5-FU (Bel-7402/5-FU) were cultured, and a xenograft was established in nude mice. MTT assays were used to detect the cell viability after XCHD treatment, and an inverted phase contrast microscope was used to observe the morphology and flow cytometry and TUNEL assays were used to determine XCHD-induced apoptosis. Western blot was used to detect Bax and Bcl-2 expressions. Cyto-ID staining was used to assess XCHD-induced autophagy, and the autophagy-related protein (LC3, p62, and beclin) was determined. Finally, the PI3K/AKT/mTOR pathway was detected.</p></div><div><h3>Results</h3><p>Bel-7402/5-FU cells were more resistant to 5-FU compared with Bel-7402 ​cells (<em>P</em> ​&lt; ​0.05), thus XCHD could inhibit the viability of Bel-7402/5-FU cells. Further, XCHD promoted Bel-7402/5-FU cell apoptosis via inducing Bax expression and deducing Bcl-2 expression <em>in vitro</em> and <em>in vivo</em>. Similarly, XCHD promoted autophagy of Bel-7402/5-FU cells by regulating related protein expression. Finally, XCHD blocked the PI3K/AKT/mTOR pathway.</p></div><div><h3>Conclusion</h3><p>XCHD induces Bel-7402/5-FU cell apoptosis and autophagy via blocking the PI3K/AKT/mTOR pathway which is one of the important mechanisms by which XCHD reverses the multidrug resistance of HCC.</p></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"4 2","pages":"Pages 178-184"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2707368823000900/pdfft?md5=d903376119286df4637d2ae12fd774fc&pid=1-s2.0-S2707368823000900-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92047742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}