Journal of Holistic Integrative Pharmacy最新文献

{"title":"Therapeutic effects of raw rhubarb on gastrointestinal complications in ischemic stroke: An integrated analysis of gut microbiota, metabolomics, and network pharmacology","authors":"Zhanhao Ye ,&nbsp;Dongmin Cao ,&nbsp;Wenxing Ao ,&nbsp;Ting Li ,&nbsp;Minghua Xian ,&nbsp;Shumei Wang","doi":"10.1016/j.jhip.2025.05.002","DOIUrl":"10.1016/j.jhip.2025.05.002","url":null,"abstract":"<div><h3>Objective</h3><div>Gastrointestinal complications (GITC) are a major cause of increased morbidity and mortality in stroke patients, significantly impairing recovery by triggering systemic inflammation and hindering brain healing. Raw Rhubarb (RR) is a commonly used traditional Chinese medicine with significant potential in treating GITC of ischemic stroke (IS). However, its therapeutic mechanisms remain largely unknown. This study aims to investigate the therapeutic effects and potential mechanisms of RR on GITC in IS through an integrated analysis of gut microbiota, metabolomics, and network pharmacology.</div></div><div><h3>Methods</h3><div>Male Sprague-Dawley rats were subjected to middle cerebral artery occlusion/reperfusion (MCAO/R) to induce IS. The rats were divided into six groups: sham, model, nimodipine-treated, and three different dose groups for RR. Neuroprotective efficacy was assessed using infarct size measurement, neurological scoring, and histopathological analysis. Gut microbiota composition was analyzed through 16S rRNA gene sequencing, while metabolomic profiling of brain and colon tissues was performed using UPLC-Q-Orbitrap HRMS/MS. Multivariate statistical methods were employed to identify the key metabolites and pathways affected by RR treatment. Correlation analysis was conducted to establish links between gut microbiota alterations and differential metabolites. Additionally, network pharmacology, molecular docking analysis, and Western blot assays were utilized to explore the molecular mechanisms underlying RR's treatment of GITC in IS.</div></div><div><h3>Results</h3><div>RR showed significant neuroprotective effects, reducing infarct volume, improving neurological scores, and restoring intestinal function compared to the model group. In addition, gut microbiota analysis revealed that RR administration reversed gut microbiota dysbiosis in MCAO/R rats by increasing the abundance of <em>Bifidobacterium</em> and <em>Lactobacillus</em> while decreasing the abundance of <em>Escherichia-Shigella</em>. Metabolomics analysis indicated that RR reversed the metabolic disturbances in MCAO/R rats by modulating arachidonic acid (AA) metabolism. Correlation analysis showed that AA and its metabolites, such as PGE2, were closely associated with <em>Bifidobacterium</em> and <em>Lactobacillus</em>. Combining metabolomics, network pharmacology, and molecular docking analysis suggested that RR might regulate AA metabolism through the PI3K/mTOR signaling pathway to treat GITC in IS. Finally, Western blot validation confirmed that RR modulates the PI3K/mTOR signaling pathway.</div></div><div><h3>Conclusion</h3><div>These findings indicate that RR holds significant promise as a therapeutic strategy for addressing GITC of IS. The protective effects mediated by RR are associated with the improvement of gut microbiota dysbiosis and metabolic disturbances.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 136-149"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial function maintenance and mitochondrial training in ageing and related diseases","authors":"Gan Gao ,&nbsp;Zhihui Xie ,&nbsp;Hongliang Huang","doi":"10.1016/j.jhip.2025.04.001","DOIUrl":"10.1016/j.jhip.2025.04.001","url":null,"abstract":"<div><div>Cellular senescence driven by mitochondrial dysfunction is a key contributor to ageing and related diseases. The decline in the quality and quantity of healthy mitochondria with ageing disrupts energy production, redox homeostasis, and intracellular signaling. Mitochondrial quality control (MQC) is a cellular self-repair mechanism that protects mitochondrial function and maintains a healthy mitochondrial network. Targeted regulation of MQC is expected to moderate the development of cellular senescence and related diseases. We explored the impact of mitochondrial function on cell fate at the molecular and organelle levels, analyzed the role of mitochondria-targeted interventions for delaying cellular senescence and ameliorating age-related diseases, and pointed out the idea of increasing the critical level of healthy mitochondria to cope with internal and external stressful stimuli and to improve the ability of self-repairing by exercising and protecting mitochondria in the long term.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 159-174"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scoping review of five selected underutilized medicinal plants of Sri Lanka: Focusing on ethnobotany, phytochemistry and bioactivities, and evaluation of their potential for novel herbal product development","authors":"Isuru Sakbo Uyangoda ,&nbsp;Mayuri Munasinghe","doi":"10.1016/j.jhip.2025.06.005","DOIUrl":"10.1016/j.jhip.2025.06.005","url":null,"abstract":"<div><div><em>Ageratum conyzoides</em> L., <em>Artocarpus gomezianus</em> Wall. ex Trécul, <em>Euphorbia hirta</em> L., <em>Plectranthus zeylanicus</em> Benth., and <em>Piper sarmentosum</em> Roxb. have long been utilized in traditional medical practices, particularly across South and Southeast Asia. Despite their significant ethnopharmacological potential to treat various disorders, these plants remain underutilized in Sri Lanka. This review aims to evaluate the habit, propagation, ethnomedicinal uses, phytochemistry, and pharmacological properties of these five underutilized medicinal plants to promote their sustainable utilization in the herbal products industry of Sri Lanka. The plants were selected based on data from pharmacopeias and interviews with traditional medical practitioners. Scientific information on their ethnomedicinal uses, phytochemical compositions, and pharmacological properties was gathered from key scientific databases, including PubMed, Scopus, ScienceDirect, and Google Scholar, as well as web references and books. This information was analyzed to assess the factors contributing to their underutilization and their potential for novel herbal product development in Sri Lanka. All five plants possess a wide range of ethnomedicinal uses and are rich in bioactive compounds, including alkaloids, terpenoids, stilbenoids, and polyphenolic compounds. These bioactive compounds have been scientifically validated for their pharmacological properties, making these plants strong candidates for the development of novel pharmaceuticals and cosmeceuticals. However, their full potential remains largely untapped, primarily due to the lack of detailed phytochemical characterization and bioactive studies specific to Sri Lanka. Further preclinical and clinical research is needed to evaluate their therapeutic outcomes within the local context. The findings of this scoping review will guide future research and encourage broader use of these underutilized plants. Promoting their use will provide a sustainable alternative to the overexploitation of commonly used medicinal plants and support effective biodiversity conservation and resource management.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 209-223"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in the role of selenium in reversing tumor multidrug resistance","authors":"Haoqiang Hu ,&nbsp;Yunjun Chen ,&nbsp;Hongtao Xu ,&nbsp;Wei Hou","doi":"10.1016/j.jhip.2025.06.004","DOIUrl":"10.1016/j.jhip.2025.06.004","url":null,"abstract":"<div><div>Multidrug resistance (MDR) is a significant challenge in the cancer therapy, with mechanisms primarily involving increased drug efflux mediated by ABC transporters, leading to reduced intracellular drug concentrations. In recent years, various selenium-containing compounds have demonstrated extensive biological activities, including chemoprevention, antioxidant or pro-oxidant effects, and regulation of the nervous and immune system activities. One of the most prominent physiological characteristics of selenium is its antioxidant capacity, which can regulate the levels of reactive oxygen species (ROS) in the body, making it a promising group for reversing MDR activity. Furthermore, research has shown that natural selenium compounds, including selenate, selenite, selenomethionine, and selenocystein, can inhibit the activity of drug resistance proteins and increase the intracellular accumulation of chemotherapeutic drugs by regulating intracellular ROS levels. For instance, sodium selenite has been shown to markedly increase the sensitivity of drug-resistant cell lines to doxorubicin, exhibiting significant antitumor efficacy and potential for reversing MDR. These findings suggest that selenium compounds hold considerable promise in addressing multidrug resistance. Consequently, this review focuses on elucidating the mechanisms of MDR and the chemical properties of selenium compounds, with particular emphasis on their activities in reversing MDR, thereby providing novel strategies for overcoming MDR in tumor cells.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 184-194"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and ex vivo / in vitro evaluation of sodium alginate/ hydroxypropyl methylcellulose films for dermal and/or transdermal delivery of p-hydroxycinnamic acid","authors":"Ayşe Pınar Yağcılar ,&nbsp;Gökçe Karaotmarlı Güven ,&nbsp;Emre Şefik Çağlar ,&nbsp;Neslihan Üstündağ Okur ,&nbsp;Panoraia I. Siafaka","doi":"10.1016/j.jhip.2025.06.002","DOIUrl":"10.1016/j.jhip.2025.06.002","url":null,"abstract":"<div><h3>Objective</h3><div>Skin diseases and chronic wounds are health problems that require solutions for health systems due to their high costs and difficulties in effective and rapid treatment. Hydroxycinnamic acid and its derivatives are powerful antioxidant molecules with widespread applications in medicine, cosmetics, and food industry. In this study, <em>p</em>-hydroxycinnamic acid was used as a potent agent for the management of skin diseases and other disorders.</div></div><div><h3>Method</h3><div>Herein, sodium alginate and hydroxypropylmethylcellulose-based films loaded with <em>p</em>-hydroxycinnamic acid at various concentrations were prepared by solvent-casting method and characterized in terms of mechanical, physicochemical, bioadhesive properties, and <em>in vitro</em> release kinetic modelling.</div></div><div><h3>Results</h3><div>The masses of the films were found to be between 16.933 ​± ​1.108 ​mg and 15.200 ​± ​0.432 mg and thicknesses between 135 ​± ​4 μm and 163 ​± ​6 μm. F1 formulation with higher sodium alginate concentration exhibited higher moisture absorption and moisture loss percentages (18.373% ​± ​2.610% and 8.281% ​± ​1.834%). In terms of water absorption, it was observed that F3 had up to 100% and F1 had the lowest absorption capacity. However, F1 degraded in a shorter time compared to other films. In terms of mechanical properties, F1 has shown that it has higher tensile strength, reaches 100% by providing continuous release with <em>in vitro</em> release studies, and has the highest bioadhesion. In addition, as a result of FTIR analysis and <em>ex vivo</em> permeation and penetration studies, the formulation F1 proved that it is suitable for dermal applications.</div></div><div><h3>Conclusion</h3><div>The developed formulations exhibited desired dermal film properties, making it a promising treatment option for dermal applications.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 175-183"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalin induces apoptosis and autophagy in resistant human hepatocellular carcinoma cell line Bel-7402/5-FU cells via PI3K/AKT pathway","authors":"Fei Li ,&nbsp;Zilin Lan ,&nbsp;Weiwei Jiang ,&nbsp;Jianheng Zhou ,&nbsp;Jiumao Lin ,&nbsp;Jinyan Zhao","doi":"10.1016/j.jhip.2025.05.001","DOIUrl":"10.1016/j.jhip.2025.05.001","url":null,"abstract":"<div><h3>Objective</h3><div>Multidrug resistance (MDR) is one main cause of chemotherapy failure. Baicalin is an important active ingredient with anticancer potential in many Chinese herbal medicines. In order to understand the function of baicalin reversing MDR in hepatocellular carcinoma (HCC) and the molecular mechanisms that underlie it, the current study was designed.</div></div><div><h3>Methods</h3><div>Bel-7402 and Bel-7402/5-FU cells were cultured, and MTT assay was applied to detect cell viability and the cross-resistance of Bel-7402/5-FU cells. The pump function, apoptosis, and autophagy were detected by flow cytometry. The related proteins were detected by Western blot assay. The PI3K agonist (740Y-P) was used to verify whether baicalin overcomes the drug resistance of HCC cells by blocking the PI3K/AKT pathway.</div></div><div><h3>Results</h3><div>The findings showed that Bel-7402/5-FU cells were cross-resistant to different chemotherapeutic drugs. Baicalin inhibited cell viability in both Bel-7402/5-FU and Bel-7402 ​cells, and baicalin increased sensitivity of Bel-7402/5-FU cells to 5-FU in time- and dose-dependent manners. Baicalin increased the accumulation of doxorubicin and rhodamine-123 in Bel-7402/5-FU cells and inhibited the protein expression of ABCG2, ABCB1, and ABCC1, associated with pump function. In addition, baicalin induced apoptosis of Bel-7402/5-FU cells via up-regulating Bax expression. Furthermore, baicalin increased autophagy through regulating LC3-Ⅱ, p62, and Beclin-1. Baicalin reversed drug resistance in Bel-7402/5-FU cells by inhibiting the PI3K/AKT pathway, which promoted autophagy and apoptosis to restore chemosensitivity.</div></div><div><h3>Conclusion</h3><div>Baicalin increased accumulation of chemotherapy drugs and induced apoptosis and autophagy in Bel-7402/5-FU cells by inhibiting the PI3K/AKT signaling pathway, that may be the important mechanism by which baicalin reverses the MDR of HCC.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 150-158"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ameliorative effects of honeysuckle extract and its major component luteolin on autism-like behaviors in the NDE1 deficiency model","authors":"Qi Zhang,&nbsp;Shenglan Gou,&nbsp;Jia Lin,&nbsp;Yinglan Zhang,&nbsp;Qiang Li","doi":"10.1016/j.jhip.2025.06.007","DOIUrl":"10.1016/j.jhip.2025.06.007","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of honeysuckle extract and its active component, luteolin, on the autistic-like behaviors and neuroinflammatory responses in NDE1-deficient autism spectrum disorder (ASD) zebrafish models. Also, to assess whether differences exist in their behavioral improvement effects and impacts on brain inflammatory factor expression levels.</div></div><div><h3>Methods</h3><div>Behavioral phenotyping (hyperactivity, stereotypic back-and-forth swimming, and 1VS6 social preference/grouping tests) and molecular analyses (quantification of NF-κB, IL6, TNFα, and IL1β) were performed on NDE1-deficient zebrafish treated with honeysuckle extract or luteolin.</div></div><div><h3>Results</h3><div>Honeysuckle extract improved two core symptoms of ASD, small circling repetitive stereotyped behavior and 1VS6 social preference behavior. While luteolin enhanced one core symptom, shoaling behavior, and one comorbid symptom, hyperactive locomotor activity. Molecularly, honeysuckle extract normalized IL6 levels, and luteolin reduced IL1β overexpression; their effects on brain inflammation in the NDE1-deficient autism model differed.</div></div><div><h3>Conclusion</h3><div>Both honeysuckle extract and luteolin demonstrated behavioral rescue and anti-neuroinflammatory effects in NDE1-deficient ASD zebrafish models. The ameliorating effects of luteolin on ASD-related behaviors and neuroinflammation are supported by literature, while the beneficial effects of honeysuckle on ASD-related behaviors represent a novel finding of this study, highlighting medicinal plants and plant-derived compounds as potential ASD therapeutics. Given honeysuckle's traditional Chinese medicinal and food uses, established safety, and superior improvement of core ASD symptoms compared to luteolin, it may offer a safer autism treatment option than luteolin-based small-molecule medication.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 195-203"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of potential markers for vitiligo based on bioinformatics and LASSO regression and prediction of Chinese medicine","authors":"Wei liang ,&nbsp;Minni Huang ,&nbsp;Yue Sun ,&nbsp;Shuyu Guan","doi":"10.1016/j.jhip.2025.06.003","DOIUrl":"10.1016/j.jhip.2025.06.003","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to use bioinformatics techniques to screen biomarkers related to vitiligo.</div></div><div><h3>Methods</h3><div>Firstly, the gene expression profiles of vitiligo were obtained from the GEO database, and differentially expressed genes (DEGs) were identified. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed on these differentially expressed genes. Through weighted gene co-expression network analysis (WGCNA), the core genes in the module most closely related to vitiligo were identified, and an intersection analysis was conducted with the DEGs. Next, a protein-protein interaction (PPI) network analysis was carried out on the intersection genes. Key genes were further screened using Cytohubba and least absolute shrinkage and selection operator (LASSO) regression analysis, and the roles of these key genes in immune cell infiltration were explored through single-sample gene set enrichment analysis (ssGSEA). In addition, the diagnostic effectiveness of the key genes was verified by the receiver operating characteristic (ROC) curve, and drugs related to the key genes were predicted using databases. Finally, the expression levels of these key genes were verified through reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot experiments.</div></div><div><h3>Results</h3><div>A total of 667 DEGs were identified, and the enrichment analysis mainly involved cell adhesion molecules, T cell receptor signaling pathway, etc. Nineteen core genes were screened out from the five algorithms of Cytohubba, and LASSO regression analysis further determined four key genes <em>(IL7R, GZMH, CD3G,</em> and <em>UBD</em>). Immune cell infiltration analysis showed that these four key genes had high expression in immune cells. The prediction results of traditional Chinese medicine showed that 15 traditional Chinese medicines were related to the key genes. The results of RT-qPCR showed that the expressions of <em>IL7R</em>, <em>GZMH</em>, and <em>CD3G</em> were significantly upregulated (^∗<em>P</em> ​&lt; ​0.05, ^∗∗<em>P</em> ​&lt; ​0.01, ^∗∗∗<em>P</em> ​&lt; ​0.001), and Western blot showed obvious expressions of <em>IL7R</em>, <em>GZMH</em>, <em>CD3G</em>, and <em>UBD</em>.</div></div><div><h3>Conclusion</h3><div>This study used bioinformatics methods to explore the biomarkers of vitiligo, and verified the potential of <em>IL7R</em>, <em>GZMH</em>, and <em>CD3G</em> as novel candidate genes through <em>in vitro</em> experiments. These genes may become new targets for the diagnosis, prognosis, and treatment of vitiligo.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 224-234"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating metabolism gene clusters and tumor immune microenvironment in head and neck squamous cell carcinoma","authors":"Meina Lian ,&nbsp;Xiaoxia Wang ,&nbsp;Zixian Huang ,&nbsp;Yudong Wang ,&nbsp;Zhiquan Huang","doi":"10.1016/j.jhip.2025.06.006","DOIUrl":"10.1016/j.jhip.2025.06.006","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the relationship between tumor metabolism and immune cell infiltration in Head and Neck Squamous Cell Carcinoma (HNSCC), aiming to identify novel biomarkers and potential therapeutic targets.</div></div><div><h3>Methods</h3><div>Seven major metabolic pathways were analyzed using Gene Set Variation Analysis (GSVA) in HNSCC cohorts to assess their correlation with overall survival (OS) and immune microenvironment characteristics. Unsupervised clustering was applied to identify metabolic subtypes, and differentially expressed metabolism-related genes (MRGs) were screened for prognostic relevance. A risk model was constructed based on 16 core MRGs. TNFAIP6 was further evaluated for its functional role through <em>in vitro</em> assays, including proliferation, migration, and invasion analyses.</div></div><div><h3>Results</h3><div>The activity of key metabolic pathways, such as glycolysis, oxidative phosphorylation, and fatty acid metabolism, significantly correlated with OS and immune infiltration patterns. Two distinct metabolic clusters (C1 and C2) were identified, with C1 associated with a more immune-enriched microenvironment. A total of 698 MRGs were linked to immune modulation and tumor progression. The risk model based on 16 MRGs effectively stratified patients by prognosis and immune infiltration status. TNFAIP6 was highly expressed in malignant cells and associated with immunosuppression, poor survival, and tumor progression. Functional experiments confirmed that TNFAIP6 knockdown inhibited tumor cell proliferation, migration, and invasion.</div></div><div><h3>Conclusion</h3><div>Metabolic reprogramming plays a critical role in shaping the immune landscape of HNSCC. TNFAIP6 represents a promising prognostic biomarker and potential therapeutic target for improving personalized treatment in HNSCC patients.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 235-248"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-derived organoids: Advancing research on bioactive natural compounds in lung cancer","authors":"Xiao Chen ,&nbsp;Xian Lin","doi":"10.1016/j.jhip.2025.06.008","DOIUrl":"10.1016/j.jhip.2025.06.008","url":null,"abstract":"<div><div>Lung cancer, the leading cause of cancer-related deaths, demands innovative models for therapy development. Bioactive natural compounds, with their structural diversity and historical therapeutic significance, remain pivotal in drug discovery for combating lung malignancies. Patient-derived organoids (PDOs) surpass conventional models by preserving tumor heterogeneity, molecular profiles, and tumor microenvironment (TME) dynamics, enabling accurate drug response prediction and personalized therapy design. Recent studies leveraging lung cancer PDOs have validated several plant-derived agents for their tumor-suppressive effects, potential for chemosensitivity enhancement, and subtype-specific efficacy. Advanced co-culture systems incorporating TME components have improved preclinical-to-clinical translatability. The technological integration of bioengineered platforms (e.g., microfluidic systems, 3D bioprinting) and artificial intelligence has further enhanced high-throughput screening and clinical correlation of drug responses. Although lung cancer PDOs exhibit inherent limitations, these advancements establish PDOs as important tools for evaluating the efficacy-toxicity profiles of bioactive natural compounds and advancing precision oncology in lung cancer.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 2","pages":"Pages 204-208"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}