Journal of Holistic Integrative Pharmacy最新文献

{"title":"Mechanistic insights into the preventive efficacy of 21-day prefeeding with β-galactosidase hydrolyzed milk in DSS-induced ulcerative colitis mice","authors":"Jinping Wang ,&nbsp;Hao Sun ,&nbsp;Lingmei Zhou ,&nbsp;Liwen Cai ,&nbsp;Bingqing Hao ,&nbsp;Zhuoya Wang","doi":"10.1016/j.jhip.2026.02.002","DOIUrl":"10.1016/j.jhip.2026.02.002","url":null,"abstract":"<div><h3>Objective</h3><div>Ulcerative colitis (UC) is a chronic inflammatory bowel disease with limited preventive strategies. Here, we investigated the 21-day preventive effects of <em>β</em>-galactosidase hydrolyzed milk (Milk <em>β</em>-gal) against Dextran Sulfate Sodium (DSS)-induced UC in mice.</div></div><div><h3>Methods</h3><div>C57BL/6 mice were randomly divided into control group (Control), DSS-induced UC model group (DSS), UC model group pretreated with regular milk group (Milk), and UC model group pretreated with <em>β</em>-galactosidase hydrolyzed milk group (Milk <em>β</em>-gal). After a 21-day pre-feeding period with milk and Milk <em>β</em>-gal, colitis was induced with DSS in the drinking water. We assessed disease severity through body weight change in mouse body weight, disease activity index (DAI) scores, and colon length. Assess colonic histopathology using hematoxylin and eosin (HE) staining. Detect changes in intestinal permeability using FITC. Use qPCR and Western blot analysis to measure the expression levels of inflammatory factors and cellular signaling pathway proteins in colonic tissue. Measure malondialdehyde (MDA) and superoxide dismutase (SOD) levels in colon tissue alongside liver and kidney injury markers in peripheral blood to assess physiological and biochemical indicators. 16S rRNA high-throughput sequencing was used to analyse changes in the gut microbiota of mouse faeces and observe microbial community structure and composition.</div></div><div><h3>Results</h3><div>Milk <em>β</em>-gal significantly attenuated colitis severity, as evidenced by reduced body weight loss, lower disease activity index (DAI), mitigated colon shortening, diminished histopathological damage, and restored colonic barrier integrity. Mechanistically, Milk <em>β</em>-gal suppressed pro-inflammatory cytokines (TNF-<em>α</em>, IL-6, IL-1<em>β</em>, and IL-33) and oxidative mediators (iNOS, COX-2) while maintaining anti-inflammatory cytokines (IL-10, TGF-<em>β</em>) at intermediate levels, suggesting a restored equilibrium between inflammatory and reparative pathways. Moreover, Milk <em>β</em>-gal reversed DSS-induced downregulation of ZO-1 and MUC-2, thereby counteracting epithelial barrier dysfunction. Following DSS administration, Milk <em>β</em>-gal restored Firmicutes/Bacteroidetes ratio, reduced Campilobacterota, and elevated mucosa-repairing <em>Helicobacter</em> and uncultured <em>Oscillospiraceae</em>.</div></div><div><h3>Conclusion</h3><div>These coordinated improvements in host responses and microbial ecology suggest that the <em>β</em>-galactosidase hydrolyzed milk may represent a promising dietary strategy for mitigating colitis in a murine model, providing a foundation for further investigation.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 33-43"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147406195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic approaches to dyslipidaemia in Unani medicine: a narrative review","authors":"Tabasum Rouf,&nbsp;Iftikhar Ahmed","doi":"10.1016/j.jhip.2026.01.002","DOIUrl":"10.1016/j.jhip.2026.01.002","url":null,"abstract":"<div><h3>Objective</h3><div>Dyslipidaemia is one of the most common metabolic disorders, strongly linked with cardiovascular disease, obesity, and other components of metabolic syndrome. In Unani medicine, this condition is understood through concepts such as <em>obesity</em> (<em>Simān-i-Mufriṭ</em>), with a predominance of phlegm (<em>Balgham</em>) and impaired hepatic temperament and has traditionally been managed by dietary regulation, regimental therapies and herbal drugs. This narrative review aims to critically evaluate the Unani conceptualisation of dyslipidaemia and to map these traditional classical concepts with contemporary lipid-modulating mechanisms<em>.</em> It further discusses the pathophysiology of dyslipidaemia, and reviews therapeutic approaches such as regimental therapies, single drugs, and compound formulations, while evaluating the strength of evidence and highlighting future research priorities for integrative management.</div></div><div><h3>Methods</h3><div>Electronic searches were conducted in databases such as PubMed, Scopus, and Google Scholar for articles published between 1980 and 2024. Classical Unani texts and authoritative pharmacopoeias were also reviewed. Search terms included <em>dyslipidaemia, hyperlipidaemia, Unani medicine, obesity, metabolic syndrome, lipid-lowering herbs, antioxidants, and anti-inflammatories.</em></div></div><div><h3>Results</h3><div>Several Unani drugs, such as <em>Emblica officinalis, Cyperus rotundus, Ocimum sanctum, Hemidesmus indicus, Anethum graveolens, Tinospora cordifolia, Myristica fragrans, Nigella sativa, Qurs tabasheer, Jawarish bisbasa</em> and many more demonstrated lipid-lowering effects through mechanisms such as antioxidant activity, anti-inflammatory effects and improvement of hepatic function. However, the strength of evidence varies considerably, with limited clinical trials available for most interventions.</div></div><div><h3>Conclusion</h3><div>Unani drugs demonstrate a plausible complementary approach for dyslipidaemia management through multi-target mechanisms aligned with modern pathophysiology. however, robust clinical trials and standardized formulations are required to establish efficacy and safety.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146192471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic activity of bioactive fractions from Momordica cymbalaria Fenzl ex Naudin leaves: In vitro, in silico and in vivo evaluation","authors":"Gopalasatheeskumar K ,&nbsp;Kalaichelvan VK ,&nbsp;Arulkumar R","doi":"10.1016/j.jhip.2026.02.011","DOIUrl":"10.1016/j.jhip.2026.02.011","url":null,"abstract":"<div><h3>Objective</h3><div>This study evaluated the antidiabetic activity of <em>Momordica cymbalaria</em> Fenzl ex Naudin (MC) leaf fractions, a traditionally used remedy in southern India, through comprehensive <em>in vitro</em>, <em>in silico</em>, and <em>in vivo</em> investigations to run scientific validation for its therapeutic potential.</div></div><div><h3>Methods</h3><div>MC leaves were extracted by cold maceration with 60% methanol and fractionated based on polarity into five fractions (MF1-MF5). Fractions underwent phytochemical screening, total phenolic and flavonoid measurement, and <em>in vitro</em> antidiabetic tests (<em>α</em>-amylase &amp; <em>α</em>-glucosidase inhibition, glucose diffusion). In rats that were driven to diabetes by streptozotocin and nicotinamide, the most active fraction (MF3, 200 and 400 mg/kg) was tested orally for 21 days. GC-MS spectrometry was used to track down the bioactive chemicals, and molecular docking studies assessed their interactions with 21 diabetes-related protein targets.</div></div><div><h3>Results</h3><div>MF3 exhibited the highest phenolic (427.33 mg/g GAE) and flavonoid (394.35 mg/g QE) content with potent <em>α</em>-amylase (IC₅₀ = 63.07 μg/mL) and <em>α</em>-glucosidase (IC₅₀ = 118.65 μg/mL) inhibition, comparable to acarbose. In diabetic rats, MF3 (400 mg/kg) expressively diminished blood glucose quantity (from 311.33 ± 43.59 to 99 ± 12.85 mg/dL, <em>P</em> &lt; 0.001), improved body weight, normalized lipid profiles, and restored hepatic antioxidant enzymes. GC-MS identified 22 compounds, with 3-Buten-2-one derivative showing excellent binding affinity (≤ −10.0 kcal/mol) to key diabetes-related targets including maltase-glucoamylase and dipeptidyl peptidase-4.</div></div><div><h3>Conclusion</h3><div>The results of this research provide credence to the long-standing belief that MC leaf fractions, and MF3 in particular, possess substantial antidiabetic properties, lending credence to the plant's traditional usage. These promising discoveries necessitate further clinical trials to verify their validity and establish the plant's medicinal effectiveness.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 153-166"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147449662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the potential medical beauty efficacy screening of acetyl hexapeptide-8 and the mechanism of regulating collagen regeneration","authors":"Zuyu Ye ,&nbsp;Xinru Wei ,&nbsp;Zongyu Miao ,&nbsp;Runan He ,&nbsp;Yongshi Guan ,&nbsp;Yuanzheng Wei ,&nbsp;Huixin Ye ,&nbsp;Meihui Wu ,&nbsp;Lei Cai ,&nbsp;Chuqin Yu","doi":"10.1016/j.jhip.2026.02.005","DOIUrl":"10.1016/j.jhip.2026.02.005","url":null,"abstract":"<div><h3>Objective</h3><div>Acetyl hexapeptide-8 (AH-8) is a synthetic polypeptide that can promote collagen regeneration; however, the underlying mechanism of this promoting effect and other potential medical beauty efficacy of AH-8 remain unknown. This study aims to explore the effect of AH-8 in promoting collagen regeneration and its underlying mechanism.</div></div><div><h3>Methods</h3><div>In this study, various zebrafish efficacy evaluation models were constructed to systematically evaluate the potential medical efficacy of AH-8. At the same time, the promoting effect and mechanism of AH-8 on collagen regeneration were explored from the messenger ribonucleic acid (mRNA) and protein level, respectively.</div></div><div><h3>Results</h3><div>AH-8 promoted the expression of collagen and elastin genes, reduced locomotor activity in zebrafish under both basal conditions and in chemically-induced behavioral models, and decreased the expression of inflammatory factors. These findings suggest that AH-8 has the potential to regulate collagen regeneration, alleviate neural excitation, reduce stinging sensations, and exert soothing and anti-inflammatory effects. RNA sequencing analysis via high-throughput sequencing revealed that differentially expressed genes between the model group and the AH-8-treated group were enriched in pathways related to organismal aging regulation (e.g., the TGF-<em>β</em> signaling pathway), and these genes were upregulated after AH-8 treatment. Integrated data from transcriptomics, qPCR, ELISA, and CRISPR-dCas9-mediated knockdown consistently demonstrated that AH-8 concentration-dependently upregulated the expression of collagen genes (<em>col1a1a, col1a1b, col1a2</em>) and the elastin gene (<em>eln1</em>). Corresponding concentration-dependent changes in key pathway genes (<em>tgfb3, tgfrb1b, smad3b, smad4a/b</em>) aligned with the transcriptome sequencing results.</div></div><div><h3>Conclusion</h3><div>AH-8 exhibits anti-wrinkle, nerve-soothing, sting-relieving, mild and non-irritating, skin-brightening, moisturizing, immediate anti-wrinkle, soothing, and anti-inflammatory efficacy. AH-8 probably promotes collagen regeneration by regulating key genes, such as <em>col1a1a, col1a1b, col1a2, eln1,</em> and <em>smad4b,</em> within the TGF<strong>-</strong><em>β</em>/Smad2/3 signaling pathway, and by influencing the expression of related factors.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 54-65"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147406180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring synergistic effects of bioactive compounds and pharmaceuticals in therapeutic applications","authors":"Idayat Adeola Akinwumi ,&nbsp;Owoola Azeezat Ambali","doi":"10.1016/j.jhip.2026.02.009","DOIUrl":"10.1016/j.jhip.2026.02.009","url":null,"abstract":"<div><div>Synergy arises when the combined effect of multiple agents exceeds the sum of their individual activities. Pharmacological and bioactive chemical synergy offers a promising approach to enhance therapeutic efficacy, reduce toxicity, and overcome drug resistance. This review explores the mechanisms underlying drug synergy, focusing on pharmacodynamic and pharmacokinetic interactions, enhanced efficacy, and potential toxicity risks. It also highlights key gaps, challenges, and future research directions. This review integrates published findings on bioactive–drug interactions, with emphasis on mechanistic pathways and therapeutic applications. Literature was retrieved using keywords on synergy and pharmacological interactions from databases including PubMed, Google Scholar, and Web of Science, last accessed on September 30, 2025, covering studies up to 2025. Evidence indicates that synergy occurs through complementary pharmacodynamic and pharmacokinetic mechanisms. Pharmacodynamic effects include multi-target modulation, receptor co-activation, and inhibition of resistance pathways, while pharmacokinetic mechanisms involve improved absorption, metabolism, and excretion. Natural sources such as plants, microbes, and marine organisms provide a rich reservoir of compounds capable of enhancing conventional treatments. Curcumin and epigallocatechin gallate have been shown to increase the efficacy of antibiotics and antifungals against resistant microorganisms. Berberine combined with metformin improves glucose regulation in metabolic disorders through overlapping mechanisms. Similarly, green tea catechins, resveratrol, and curcumin enhance chemotherapy effectiveness while reducing adverse effects in oncology. The integration of natural bioactive compounds with conventional drugs represents a powerful strategy for developing next-generation therapies. However, there are still limitations, including challenges with bioavailability, safety profiles, and consistency across studies. Future research should prioritize pharmacokinetic modeling, advanced delivery systems, and systems biology approaches to optimize bioactive–drug synergy and translate laboratory findings into effective clinical applications.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 102-110"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147406502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ToC","authors":"","doi":"10.1016/S2707-3688(26)00029-4","DOIUrl":"10.1016/S2707-3688(26)00029-4","url":null,"abstract":"","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages i-ii"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147714003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut–brain axis in neurological disorders: mechanistic links and regulatory role of Chinese herbal medicines","authors":"Seema Sharma,&nbsp;Sampat Singh Tanwar","doi":"10.1016/j.jhip.2026.02.004","DOIUrl":"10.1016/j.jhip.2026.02.004","url":null,"abstract":"<div><div>The gut–brain axis (GBA) is a bidirectional communication network integrating neural, immune, endocrine, and metabolic pathways that link the gastrointestinal tract and the central nervous system. Increasing evidence implicates gut microbiota dysbiosis in the pathogenesis of neurological disorders, including Alzheimer's disease, Parkinson's disease, multiple sclerosis, autism spectrum disorders, depression, and schizophrenia. This review synthesizes preclinical and clinical evidence to elucidate GBA-mediated mechanisms of neurological dysfunction and critically evaluates the therapeutic potential of Traditional Chinese herbal medicines (TCHMs). We highlight how dysbiosis disrupts immune signaling, microbial metabolite production, intestinal and blood–brain barrier integrity, and neurotransmitter pathways involving serotonin, dopamine, γ-aminobutyric acid, and glutamate, thereby driving neuroinflammation, oxidative stress, and neuronal injury. Particular emphasis is placed on the ability of TCHMs to restore microbial homeostasis, enhance short-chain fatty acid production, strengthen gut barrier function, and regulate neuroendocrine pathways, notably the hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes. These multi-target actions are consistently associated with improved cognitive, behavioral, and neuroinflammatory outcomes across experimental models. The review further identifies emerging synergistic strategies combining TCHMs with microbiota-targeted interventions, such as probiotics and dietary modulation, which enhance correction of dysbiosis and attenuation of neuroinflammatory cascades. Nonetheless, heterogeneity in study design, herbal formulations, and microbiome profiling limits clinical translation. Future progress will require standardized methodologies, multi-omics integration, and precision-based approaches. Overall, this review positions Chinese herbal medicines as promising systems-level modulators of the gut–brain axis for neurological disease management.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 66-90"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147406196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning models for predicting pharmacokinetics and pharmacodynamics: A step toward personalized dosing","authors":"Paridhi Gupta,&nbsp;Rishab Chaudhary,&nbsp;Falguni Goel","doi":"10.1016/j.jhip.2026.02.007","DOIUrl":"10.1016/j.jhip.2026.02.007","url":null,"abstract":"<div><div>Machine learning (ML) has become increasingly integrated within pharmacokinetics (PK) and pharmacodynamics (PD) modeling in recent years, and represents a new paradigm in precision medicine, enabling medically-relevant prediction of drug profile and drug response from data. Many conventional PK/PD models and tools have historically been either incapable of accommodating the complexity of PK/PD as demonstrated in individual patients, for whom genetic, physiological, and environmental factors can increase variability with drug disposition, effectiveness, and safety; therefore, requiring some level of medical judgement, often based on rich personal experience, subjectivity, and uncertainty. Machine learning can efficiently and effectively leverage large datasets of heterogeneous data, unveil subtextual behavior patterns, and facilitate the development of predictive models as research tools that are suited for personalized dosage regimens. In this review, we comprehensively summarize advances in recent ML algorithms such as random forests, support vector machines, neural networks and ensemble methods applied to PK/PD prediction and prediction as parameterized, and in estimation, of ADME parameters, as well as efficacy and toxicity profiles. We also discuss critical challenges such as data quality and representativeness, how the properties of medical research impact model interpretation, clinical integration of ML, and future elements of research opportunities. In summary, bridging computational intelligence with pharmacology is an important step forward for inferences for personalized and individualized optimized therapies to improve clinical outcomes via definitive interventions.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 223-235"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147540190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric analysis of research on plant exosomes/extracellular vesicles: current status, hotspots, and trends","authors":"Fuxing Shu ,&nbsp;Fengjian Liu ,&nbsp;Leilei Jin ,&nbsp;Guoguang Chen ,&nbsp;Jishuang Chen","doi":"10.1016/j.jhip.2026.03.005","DOIUrl":"10.1016/j.jhip.2026.03.005","url":null,"abstract":"<div><div>This study performed a comprehensive bibliometric analysis on 1373 valid publications of plant exosomes/extracellular vesicles from 2010 to 2026, retrieved from CNKI, WoSCC and PubMed and screened manually. Microsoft Excel 2019 and CiteSpace 6.3. R1 were used for data analysis, including literature trend statistics, collaborative network mapping, and keyword and co-cited reference mining, to clarify the field's research status, hotspots and evolutionary trends. The results showed a significant exponential growth in annual publications (R² = 0.983), peaking in 2025 (25.1% of total output), indicating the field's rapid development. Geographically, research spans 55 countries/regions, with China (405 publications, leading in output) and the US (179 publications, centrality = 0.6, core bridging role) as the core, forming a close collaboration; Germany, Italy and South Korea are important nodes, constructing a multi-country cross-regional collaboration pattern. Cited journals reflect strong interdisciplinarity, with Scientific Reports ranking first in citations, and high-impact comprehensive journals (Science, Nature) and professional journals in plant science, nanomedicine both being highly cited. Author collaboration presents a core-periphery structure, with Cai Qiang, Emiliani Carla as core scholars; different teams focus on mechanism-based basic research and function-based applied research, complementing each other. Keyword analysis identified drug delivery, anti-tumor therapy and plant-derived exosome-like nanovesicles as core hotspots; the research focus has shifted from early structural identification and basic mechanism research to molecular regulation exploration, and recently to application-oriented research, with plant-derived exosome-related keywords showing continuous citation bursts since 2025. Co-cited references revealed the field's core knowledge base formed in 2017–2022, covering biological function validation, structural characterization and engineering application. Further analysis found the field is supported by isolation technique optimization, green drug delivery platform development and clinical indication expansion; plant exosomes have unique advantages over animal-derived ones in cost, biocompatibility and gastrointestinal stability. This study comprehensively outlines the global research landscape of plant exosomes/extracellular vesicles, revealing its application-driven, mechanism-supported development trend, and provides scientific references for subsequent fundamental and translational research in this field.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 212-222"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147540191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From traditional herb to modern therapeutics: phytochemistry, pharmacology, toxicology, and clinical translation challenges of Cyperus rotundus L","authors":"Yuyang Wang ,&nbsp;Mengyuan Lv ,&nbsp;Yingfei Liu ,&nbsp;Yongfu Wang ,&nbsp;Nan Zhang ,&nbsp;Di Chen","doi":"10.1016/j.jhip.2026.01.001","DOIUrl":"10.1016/j.jhip.2026.01.001","url":null,"abstract":"<div><div><em>Cyperus rotundus</em> L. (<em>C. rotundus</em>.), widely distributed in tropical and temperate regions, is a perennial herb with a long history of application in traditional medical systems for treating emotional disturbances, digestive disorders, and gynecological diseases. Despite its extensive traditional use, a systematic integration of its modern phytochemistry and pharmacology is essential for its further development. This review summarizes the research progress over the past decade regarding the chemical constituents, pharmacological mechanisms, and toxicological profile of <em>C. rotundus</em>. Phytochemical investigations have revealed a diverse array of bioactive compounds, primarily volatile oils (e.g., <em>α</em>-cyperone, cyperotundone) and non-volatile constituents such as flavonoids and phenolic acids. Pharmacological studies have corroborated its broad therapeutic potential, demonstrating significant antitumor, antidepressant, anti-inflammatory, antioxidant, and reproductive-regulating activities. Toxicological assessments indicate that aqueous and ethanolic extracts possess a favorable safety profile with high LD₅₀ values, although systematic clinical safety data remain limited. Finally, future perspectives are proposed to facilitate the clinical translation of this herb, emphasizing the importance of standardized quality control, structure-activity relationship (SAR) elucidation, nanotechnology-based delivery, and rigorous randomized controlled trials (RCTs).</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"7 1","pages":"Pages 11-22"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147406193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}