Infectious Medicine最新文献

{"title":"Metal nanoparticles as inhibitors of enzymes and toxins of multidrug-resistant Staphylococcus aureus","authors":"Amruta A. Joshi,&nbsp;Ravindra H. Patil","doi":"10.1016/j.imj.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.imj.2023.11.006","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> is an aerobic Gram-positive spherical bacterium known to cause a broad range of infections worldwide. It is a major cause of infective skin and soft infections and severe and life-threatening conditions, such as pneumonia, bloodstream infections, and endocarditis. The emergence of drug-resistant strains of <em>S aureus</em>, particularly methicillin-resistant <em>S aureus</em> (MRSA), has become a significant concern in the healthcare community. Antibiotic-resistant <em>S aureus</em> is commonly acquired in hospitals and long-term care facilities. It often affects patients with weakened immune systems, those undergoing invasive medical procedures, or those who have been hospitalized for extended periods. In the US, <em>S aureus</em> is known to cause potentially fatal illnesses, such as toxic shock syndrome (TSS) and acute-onset toxic shock syndrome (TSS), which are characterized by fever and hypotension. It develops resistance to antibiotics through several mechanisms, such as the production of enzymes that inactivate antibiotics, target site modification, efflux pumps, and plasmid-mediated resistance. Therefore, preventing the spread of drug-resistant <em>S aureus</em> is needed, and there is an urgent need to explore novel approaches in the development of anti-<em>staphylococcal</em> agents. This article reviews the principal infections caused by <em>S aureus,</em> major virulence factors, mechanisms of resistance development, and nanotechnology-based solutions for the control of drug-resistant <em>S aureus</em>.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 294-307"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000618/pdfft?md5=05d120ded6f685c00dd65fb127a51b4b&pid=1-s2.0-S2772431X23000618-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138739236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chikungunya in Zhejiang Province, Southeast China","authors":"Jiangping Ren ,&nbsp;Feng Ling ,&nbsp;Ying Liu ,&nbsp;Jimin Sun","doi":"10.1016/j.imj.2023.11.005","DOIUrl":"https://doi.org/10.1016/j.imj.2023.11.005","url":null,"abstract":"<div><h3>Background</h3><p>Chikungunya is emerging and reemerging word-widely in the past decades. It is non-endemic in Zhejiang Province, Southeast China. Aedes albopictus, one of major vectors of chikungunya, is widely-distribution in Zhejiang, and autochthonous transmission is possible after introducing chikungunya virus.</p></div><div><h3>Methods</h3><p>Retrospectively collected the epidemiological, clinical and genetic data of chikungunya and conducted the descriptive analysis and gene sequence analysis.</p></div><div><h3>Results</h3><p>From 2008 to 2022, 29 chikungunya cases, including 26 overseas imported and 3 local cases, were reported and no cases died of chikungunya. More than half of the imported cases (53.85%) were from Southeast Asia. Seasonal peak of the imported cases was noted between August and September, and 42.31% cases onset in those 2 months. Eight prefecture-level cities and 16 counties reported cases during the study period, with Jinghua (27.59%) and Hangzhou (24.14%) reporting the largest number of cases. The 3 local cases were all reported in Qujiang, Quzhou in 2017. For imported cases, the male-female gender ratio was 2.71:1, 20–30 years old cases (46.15%) and commercial service (42.31%) accounted for the highest proportion. Clinically, fever (100%), fatigue (94.44%), arthralgia (79.17%), headache (71.43%) and erythra (65.22%) were the most common reported symptoms. Eight whole-genome sequences were obtained and belonged to East/Central/South African (ECSA) or Asian genotype.</p></div><div><h3>Conclusions</h3><p>With the change of immigration policy, the surveillance of chikungunya should be strengthened and the ability of the case discovery and diagnosis should be improved in Zhejiang in the post-COVID-19 era.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 315-323"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000606/pdfft?md5=292246ca3cd6d34e0030b537eec7a5e4&pid=1-s2.0-S2772431X23000606-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138739237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymyxin sensitivity/resistance cosmopolitan status, epidemiology and prevalence among O1/O139 and non-O1/non-O139 Vibrio cholerae: A meta-analysis","authors":"Bright E. Igere ,&nbsp;Hope Onohuean ,&nbsp;Declan C. Iwu ,&nbsp;Etinosa O. Igbinosa","doi":"10.1016/j.imj.2023.11.004","DOIUrl":"https://doi.org/10.1016/j.imj.2023.11.004","url":null,"abstract":"<div><p>Resistance/sensitivity to polymyxin-B (PB) antibiotic has been employed as one among other epidemiologically relevant biotyping-scheme for <em>Vibrio cholerae</em> into Classical/El Tor biotypes. However, recent studies have revealed some pitfalls bordering on PB-sensitivity/resistance (PBR/S) necessitating study. Current study assesses the PBR/S cosmopolitan prevalence, epidemiology/distribution among O1/O139 and nonO1/nonO139 <em>V. cholerae</em> strains. Relevant databases (Web of Science, Scopus and PubMed) were searched to retrieve data from environmental and clinical samples employing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Random-effect-model (REM) and common-effect-model (CEM) of meta-analysis was performed to determine prevalence of PBR/S <em>V. cholerae</em> strains, describe the cosmopolitan epidemiological potentials and biotype relevance. Heterogeneity was determined by meta-regression and subgroup analyses. The pooled analyzed isolates from articles (7290), with sensitive and resistance are 2219 (30.44%) and 5028 (69.56%). Among these PB-sensitive strains, more than 1944 (26.67%) were O1 strains, 132 (1.81%) were nonO1 strains while mis-reported Classical biotype were 2080 (28.53) respectively indicating potential spread of variant/dual biotype. A significant PB-resistance was observed in the models (CEM = 0.66, 95% CI [0.65; 0.68], <em>p</em>-value = 0.001; REM = 0.83 [0.74; 0.90], <em>p</em> = 0.001) as both models had a high level of heterogeneity (<em>I</em>² = 98.0%; <span><math><mrow><msubsup><mrow></mrow><mrow><mi>d</mi><mi>f</mi><mo>=</mo><mn>33</mn></mrow><mn>2</mn></msubsup><mo>=</mo><mn>1755.09</mn></mrow></math></span><span><math><mrow><mo>,</mo><mspace></mspace><mi>Q</mi><mi>p</mi><mo>=</mo><mn>2.4932</mn></mrow></math></span>). Egger test (<em>z</em> = 5.4017, <em>p</em> &lt; 0.0001) reveal publication bias by funnel plot asymmetry. The subgroup analysis for continents (Asia, Africa) and sources (acute diarrhea) revealed (98% CI (0.73; 0.93); 55% CI (0.20; 0.86)), and 92% CI (0.67; 0.98). The Epidemiological prevalence for El tor/variant/dual biotype showed 88% CI (0.78; 0.94) with O1 strains at 88% CI (0.78; 0.94). Such global prevalence, distribution/spread of phenotypes/genotypes necessitates updating the decades-long biotype classification scheme. An antibiotic stewardship in the post antibiotic era is suggestive/recommended. Also, there is need for holistic monitoring/evaluation of clinical/epidemiological relevance of the disseminating strains in endemic localities.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 283-293"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X2300059X/pdfft?md5=23a67f0f06d27589af7112ba4f777a16&pid=1-s2.0-S2772431X2300059X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138739235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The awareness and acceptance of HPV vaccines among parents of primary and junior high school students in China: a meta-analysis","authors":"Meimei Cui ,&nbsp;Yun Wang ,&nbsp;Zhen Liu ,&nbsp;Congxiao Liu ,&nbsp;Tianhua Niu ,&nbsp;Dan Zhou ,&nbsp;Ming Zhao ,&nbsp;Min Zhang ,&nbsp;Jingjing Jia ,&nbsp;Limei Luo","doi":"10.1016/j.imj.2023.11.003","DOIUrl":"https://doi.org/10.1016/j.imj.2023.11.003","url":null,"abstract":"<div><p>The awareness and acceptance of the human papillomavirus (HPV) vaccines among Chinese primary and junior high school students is limited. A meta-analysis was conducted to evaluate the awareness of HPV and HPV vaccines, as well as the acceptance of HPV vaccines, providing evidence-based strategies to promote HPV vaccination. Based on the databases of CNKI, Wanfang, VIP, PubMed, Embase, and Cochrane library, the literatures about the awareness of HPV and HPV vaccines, as well as the acceptance of HPV vaccines among parents of primary and junior high school students were collected from the inception to June 2023. Subgroup analysis was used to find the source of heterogeneity. Publication bias was evaluated using funnel plots and Egger's test. Fifteen literatures with 21,853 participants were included. The pooled HPV awareness, HPV vaccine awareness and acceptance rates among parents of primary and junior high school students in China were 42.90% (95% CI: 33.34%–52.47%), 28.11% (95% CI: 18.20%–43.41%), and 55.29% (95% CI: 45.85%–64.36%), respectively. The survey period and the proportion of female parents were the heterogeneity in awareness of HPV and HPV vaccines, as well as acceptance of HPV vaccines by subgroup analysis. Additionally, regional distribution emerged as another significant source of heterogeneity in HPV vaccine acceptance. The primary cause for parents' reluctance to vaccinate their children was theirs worries about the safety of the vaccines (66.21%). Though the awareness of HPV and its vaccines was low among parents of primary and junior high school students in China, the acceptance of HPV vaccines was relatively high. Strengthening health education and publicity was crucial to enhance awareness and acceptance, promoting HPV vaccination for effective cervical cancer prevention.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 273-282"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000588/pdfft?md5=cccda5691275f128a4c366331989f0fa&pid=1-s2.0-S2772431X23000588-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138739234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report","authors":"Emine E. Alieva ,&nbsp;Muniver T. Gafarova ,&nbsp;Eugene I. Bondarenko ,&nbsp;Igor A. Dovgan ,&nbsp;Irina I. Osiptchuk ,&nbsp;Marina E. Eremeeva","doi":"10.1016/j.imj.2023.09.004","DOIUrl":"10.1016/j.imj.2023.09.004","url":null,"abstract":"<div><p>Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 <em>Rickettsia</em> protein genes. The strain causing clinical illness was characterized as <em>Rickettsia conorii</em> subspecies <em>conorii</em> Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management<em>.</em></p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 338-342"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000552/pdfft?md5=5e6e114e348e4112265cef7e4de1e575&pid=1-s2.0-S2772431X23000552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136160163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life threatening rickettsiosis and the role of hemophagocytic lymphohistiocytosis syndrome (HLH): Case report of a 21-year-old woman","authors":"Marine Chancel ,&nbsp;Ali Dadban ,&nbsp;Adrien Chan Sui Ko ,&nbsp;Florence Dupont ,&nbsp;Anna Potereau ,&nbsp;Rodrigue Wankap ,&nbsp;Yoann Zerbib ,&nbsp;Jean-Philippe Lanoix","doi":"10.1016/j.imj.2023.11.008","DOIUrl":"10.1016/j.imj.2023.11.008","url":null,"abstract":"<div><p>Hemophagocytic lymphohistiocytosis (HLH) syndrome is an uncontrolled activation of macrophages, causing multiorgan dysfunction. The prognosis depends on the cause and the delay of diagnosis and treatment. Several infections can cause HLH, including rickettsia, a gram-negative bacterium. The diagnosis of rickettsia is based on clinical signs, including fever, headache, rash and sometimes tick bite site. The importance of an early diagnosis of rickettsia is the key. We present a case of rickettsia infection complicated with severe HLH occurring in a 21-year-old woman. The diagnosis was confirmed by serology 4 weeks after admission. She was treated with immunoglobulin, corticosteroids, and doxycycline with a favorable outcome.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"3 1","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000631/pdfft?md5=c8c4a97d8f662bfaab99f85164d4831d&pid=1-s2.0-S2772431X23000631-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139301891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa: Infections and novel approaches to treatment “Knowing the enemy” the threat of Pseudomonas aeruginosa and exploring novel approaches to treatment","authors":"Nikhil Sathe ,&nbsp;Peter Beech ,&nbsp;Larry Croft ,&nbsp;Cenk Suphioglu ,&nbsp;Arnab Kapat ,&nbsp;Eugene Athan","doi":"10.1016/j.imj.2023.05.003","DOIUrl":"https://doi.org/10.1016/j.imj.2023.05.003","url":null,"abstract":"<div><p><em>Pseudomonas aeruginosa</em> is an aerobic Gram-negative rod-shaped bacterium with a comparatively large genome and an impressive genetic capability allowing it to grow in a variety of environments and tolerate a wide range of physical conditions. This biological flexibility enables the <em>P. aeruginosa</em> to cause a broad range of infections in patients with serious underlying medical conditions, and to be a principal cause of health care associated infection worldwide. The clinical manifestations of <em>P. aeruginosa</em> include mostly health care associated infections and community-acquired infections. <em>P. aeruginosa</em> possesses an array of virulence factors that counteract host defence mechanisms. It can directly damage host tissue while utilizing high levels of intrinsic and acquired antimicrobial resistance mechanisms to counter most classes of antibiotics. <em>P. aeruginosa</em> co-regulates multiple resistance mechanisms by perpetually moving targets poses a significant therapeutic challenge. Thus, there is an urgent need for novel approaches in the development of anti-<em>Pseudomonas</em> agents. Here we review the principal infections caused by <em>P. aeruginosa</em> and we discuss novel therapeutic options to tackle antibiotic resistance and treatment of <em>P. aeruginosa</em> infections that may be further developed for clinical practice.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 3","pages":"Pages 178-194"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50183419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polypeptides inhibit HIV-1 replication by interfering viral Vpu-mediated tetherin degradation","authors":"Shuai Chang ,&nbsp;Lifeng Cai ,&nbsp;Yongchang Yang ,&nbsp;Binlian Sun ,&nbsp;Jingyun Li ,&nbsp;Jie Liu ,&nbsp;Lin Li","doi":"10.1016/j.imj.2023.08.001","DOIUrl":"https://doi.org/10.1016/j.imj.2023.08.001","url":null,"abstract":"<div><h3>Background</h3><p>HIV-1 Vpu acts by counteracting the tethering function of tetherin and resulting in the release of HIV-1 virion. Disrupting Vpu-tetherin interactions may provide a promising new target for antiretroviral therapy.</p></div><div><h3>Methods</h3><p>Polypeptides that covered the amino acid sequence on the interface of Vpu-tetherin complex were designed. Phenotypic susceptibilities and cellular toxicities to the polypeptides were measured. The mechanisms of the anti-HIV-1 polypeptides were determined by the Western blot analysis and laser confocal scanning. Seven 20-mer polypeptides from wild-type Vpu amino acid sequence were designed.</p></div><div><h3>Results</h3><p>We report the design and identification of 3 novel anti-HIV-1 polypeptides that derived from Vpu sequence which can efficiently inhibit HIV-1 infection. A pilot mechanism study showed that the active polypeptide could counteract Vpu-mediated tetherin downregulation. Laser confocal image scanning study showed that the polypeptides bound on the cell surface with a receptor specific binding manner, which may target tetherin that expressed on cell surface.</p></div><div><h3>Conclusion</h3><p>Our work provided first evidence that counteracting Vpu-mediated tetherin downregulation could be a target for novel anti-HIV-1 drug design. Future works to provide direct evidence of inhibitors interact with tetherin at atomic resolution and the development of small molecules inhibitors targeting Vpu-tetherin interactions may open a new avenue for novel antiretroviral therapy.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 3","pages":"Pages 224-228"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50183424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first laboratory-confirmed neonatal Mpox infection in Sri Lanka","authors":"Thulani Thivanshika Pattiyakumbura,&nbsp;Denagama Vitharanage Rishan Geeth Ruwan,&nbsp;Jinadari Maduwanthi Munasinghe,&nbsp;Gamage Don Diluk Chathuranga,&nbsp;Janaki Indira Abeynayake","doi":"10.1016/j.imj.2023.08.003","DOIUrl":"https://doi.org/10.1016/j.imj.2023.08.003","url":null,"abstract":"<div><p>In 2022–2023, a global outbreak of Mpox was reported especially in nonendemic countries. We report the first laboratory-confirmed neonatal case of Mpox infection complicated by bronchopneumonia in Sri Lanka.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 3","pages":"Pages 237-240"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50183422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of neutralizing antibodies for cross-protection against West Nile virus and Japanese encephalitis virus","authors":"Meng-Jie Yang ,&nbsp;Hao-Ran Luo ,&nbsp;Zhen-Yu Fan ,&nbsp;Yu-Xiang Feng ,&nbsp;Ning Wei ,&nbsp;Bi-Bo Zhu ,&nbsp;Jing Ye ,&nbsp;Sheng-Bo Cao ,&nbsp;You-Hui Si","doi":"10.1016/j.imj.2023.09.001","DOIUrl":"https://doi.org/10.1016/j.imj.2023.09.001","url":null,"abstract":"<div><h3>Background</h3><p>West Nile virus is a severe zoonotic pathogen that can cause severe central nervous system symptoms in humans and horses, and is fatal for birds, chickens and other poultry. With no specific drugs or vaccines available, antibody-based therapy is a promising treatment. This study aims to develop neutralizing antibodies against West Nile virus and assess their cross-protective potential against Japanese encephalitis virus.</p></div><div><h3>Methods</h3><p>Monoclonal antibodies against WNV and JEV were isolated by hybridoma technology. The therapeutic efficacy of these antibodies was evaluated using a mouse model, and a humanized version of the monoclonal antibody was generated for potential human application.</p></div><div><h3>Results</h3><p>In this study, we generated eight monoclonal antibodies that exhibit neutralizing activity against WNV. Their therapeutic effects against WNV were validated both in vivo and in vitro. Among these antibodies, C9-G11-F3 also exhibited cross-protective activity against JEV. We also humanized the antibody to ensure that it could be used for WNV infection treatment in humans.</p></div><div><h3>Conclusion</h3><p>This study highlights the importance of neutralizing antibodies as a promising approach for protection against West Nile virus infection and suggests their potential utility in the development of therapeutic interventions.</p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 3","pages":"Pages 212-223"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50183425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}