Infectious Medicine最新文献

{"title":"Japanese encephalitis virus reprograms host pyrimidine metabolism to facilitate viral replication","authors":"Jun Gu,&nbsp;Chencheng Fan,&nbsp;Shengxian Xiang,&nbsp;Youhui Si,&nbsp;Bibo Zhu,&nbsp;Shengbo Cao,&nbsp;Jing Ye","doi":"10.1016/j.imj.2026.100274","DOIUrl":"10.1016/j.imj.2026.100274","url":null,"abstract":"<div><h3>Background</h3><div>Pyrimidine metabolism is crucial for the replication of viruses and the functionality of host cells. However, the cell-type-specific organization of this metabolism in the central nervous system and its dysregulation during Japanese encephalitis virus (JEV) infection remain poorly understood. Here, we aimed to characterize the cell-type-specific landscape of pyrimidine metabolism in the CNS and uncover pyrimidine metabolic reprogramming during JEV infection.</div></div><div><h3>Methods</h3><div>Single-cell RNA sequencing data from normal and JEV-infected mouse brains were analyzed to profile the expression of pyrimidine metabolic genes across neuronal, glial, and vascular cell types. Functional validation of these metabolic pathways on JEV replication was performed in Neuro-2a cells and primary mouse brain-derived mixed neuron/glia cultures using pharmacological inhibitors, metabolite supplementation, and virological assays, including quantitative reverse transcription polymerase chain reaction, immunoblotting, and plaque assay.</div></div><div><h3>Results</h3><div>We defined a universal core program for pyrimidine homeostasis alongside specialized, function-oriented metabolic programs across neurons, glia, and vascular cells in the normal mouse brain. JEV infection triggered cell-intrinsic reprogramming of this metabolic network, with viral replication critically depending on the <em>de novo</em> pyrimidine biosynthesis pathway in neurons. Pharmacological inhibition of the key enzyme dihydroorotate dehydrogenase significantly suppressed JEV replication. Furthermore, JEV replication created a metabolic dependency on exogenous glutamine and enhanced glutamate oxaloacetate transaminase-mediated aspartate synthesis for precursor acquisition.</div></div><div><h3>Conclusions</h3><div>JEV rewires host brain pyrimidine metabolism in a cell-type-specific manner, revealing distinct metabolic dependencies on <em>de novo</em> pyrimidine biosynthesis, exogenous glutamine uptake, and glutamate oxaloacetate transaminase-mediated aspartate synthesis in neurons and highlighting potential targets for future antiviral strategies. By resolving these alterations at single-cell resolution, this study also provides a cell-type-resolved pyrimidine metabolic landscape of both the normal and JEV-infected brain.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 3","pages":"Article 100274"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148958315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of the affinity and neutralization abilities of human monoclonal antibodies against bunyavirus SFTSV glycoprotein C","authors":"Jingxian Liu,&nbsp;Yuhan Ding,&nbsp;Yuheng Zhu,&nbsp;Ying Chi,&nbsp;Chao Huang,&nbsp;Yin Chen,&nbsp;Fengjuan Shi,&nbsp;Yan Xu,&nbsp;Minghui Fu,&nbsp;Lunbiao Cui,&nbsp;Yongjun Jiao","doi":"10.1016/j.imj.2026.100273","DOIUrl":"10.1016/j.imj.2026.100273","url":null,"abstract":"<div><h3>Background</h3><div>Severe fever with thrombocytopenia syndrome (SFTS) caused by severe fever with thrombocytopenia syndrome virus (SFTSV) has become a persistent threat to public health. Since neutralizing monoclonal antibody therapy can invoke an immediate and effective passive immunity, endeavours made to discover the therapeutic monoclonal antibodies should be worthwhile. This study aimed to screen and purify the human monoclonal antibodies (hmAbs) against SFTSV glycoprotein C and characterize the properties of the hmAbs.</div></div><div><h3>Methods</h3><div>Human monoclonal antibodies against SFTSV glycoprotein C (Gc) were prepared from the convalescent SFTS patients’ lymphocytes using the phage display technology. A single-chain variable fragment antibody library against SFTSV Gc was successfully constructed, and two specific single-chain variable fragment antibodies were screened and expressed as hmAbs immunoglobulin G (IgG) (hIgG4 and hIgG5).</div></div><div><h3>Results</h3><div>Enzyme-linked immunosorbent assay, Western blot and immunofluorescence assay test results confirmed that both hIgG4 and hIgG5 specifically bound to SFTSV Gc antigen and intact SFTSV virions. The neutralization test showed that both hmAbs exhibited broad-spectrum neutralizing activity against three SFTSV strains with different genotypes (A, E and F) <em>in vitro</em>. By analyzing the RNA level of M segment by reverse transcription-polymerase chain reaction, the SFTSVs’ RNA levels were decreased, specifically, the RNA of SFTS virus JS3, SFTS virus JS4 and <em>Phlebovirus</em> JS2010-014 was reduced by 79%, 80% and 87% respectively for hIgG4, and the value was 88%, 87% and 89% for hIgG5. According to the observation of the cytopathic effect, the minimal protective concentration of hIgG4 and hIgG5 was 62.5 μg/mL and 31.25 μg/mL respectively.</div></div><div><h3>Conclusions</h3><div>hIgG4 and hIgG5 possess potent neutralizing effects, showing great potential as candidate therapeutic hmAbs for SFTS treatment.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 3","pages":"Article 100273"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148958351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convalescent plasma for persistent symptomatic COVID-19 infection in immune-suppressed patients – a case series","authors":"Daniel Ronen,&nbsp;Mutaz Karameh,&nbsp;Dan Padawer,&nbsp;Nuha Alsharif,&nbsp;Abed Elghaffar Yaghmour,&nbsp;Hila Elinav,&nbsp;Zvi Gregorio Fridlender","doi":"10.1016/j.imj.2026.100280","DOIUrl":"10.1016/j.imj.2026.100280","url":null,"abstract":"<div><div>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection progresses from an initial direct viral cell injury to an immune-mediated response. In most cases, the immune response results in viral clearance and recovery after a mild disease. Severe infections seem to result mainly from uncontrolled immune-mediated damage. Accordingly, initial treatment involves an antiviral strategy, while late severe infection is treated with anti-inflammatory medication. Immunocompromised patients have reduced vaccine efficacy and reduced ability to mount an appropriate immune response to SARS-CoV-2 infection, making them extremely susceptible to prolonged and severe infections. For these patients, standard treatment protocols need further investigation. In this retrospective case series, we investigated the effect of convalescent plasma and late antiviral treatment in twenty-three immunosuppressed patients, mostly pre-vaccinated (91%), presenting with symptomatic prolonged SARS-CoV-2 infection. All patients received initial antiviral treatment at the time of the first confirmed SARS-CoV-2 infection but continued to have symptoms. Convalescent plasma in addition to other treatments, including antivirals, resulted in significant improvement in 22 patients. Statistically significant improvement was detected in oxygen supplementation requirement (69.5% vs. 48%, <em>p</em> = 0.025), fever (61% ± 0.96 vs. 4% ± 0.42, <em>p</em> = 0.001), and C-reactive protein (9.6 ± 7.64 vs. 5.05 ± 5.05, <em>p</em> = 0.007). Interestingly, increases in white blood cell counts were found to be good predictors of improvement in this population. Our data suggest that CCP and antiviral treatment in the later phases of coronavirus disease 2019 may be necessary for immunosuppressed individuals with prolonged disease.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 3","pages":"Article 100280"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148958452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A generalized multi-population model for Ebola virus transmission incorporating environmental reservoir dynamics","authors":"Muhammad Farhan Hakeem,&nbsp;Shajar Abbas,&nbsp;Imran Siddique,&nbsp;Ilyas Khan,&nbsp;Mohsen Bakouri,&nbsp;Rashid Jan,&nbsp;Rakhmonjon Akhmadjonov,&nbsp;Rasul Turaev","doi":"10.1016/j.imj.2026.100278","DOIUrl":"10.1016/j.imj.2026.100278","url":null,"abstract":"<div><h3>Background</h3><div>Ebola virus disease (EVD) remains a serious public health hazard due to high mortality, multiple modes of transmission, and persistence in the environment. The existing Ebola transmission models have been constrained to a single or two-population framework, which are insufficient to capture multiple-population interaction transmission structures.</div></div><div><h3>Methods</h3><div>This study proposes a multi-population autonomous Ebola transmission model with Susceptible, Exposed, Infectious, Hospitalized, Recovered, and Deceased infected corpse compartments and a shared viral reservoir in the environment. This model proposes that transmission is direct, hospital-acquired, corpse-mediated, and indirect environmental, and these modes are documented by a bilinear incidence formulation.</div></div><div><h3>Results</h3><div>Positivity and boundedness properties of solutions were established via a mathematical analysis, and the basic reproduction number was derived using the next-generation matrix approach. The analytical findings show that whereas supercritical transmission results in persistent behaviour and the occurrence of endemic equilibria under the suggested parameters, the disease-free equilibrium is stable under the subcritical transmission condition.</div></div><div><h3>Conclusions</h3><div>The numerical simulations support the applicability of the suggested multi-population paradigm for comprehending threshold-dependent Ebola transmission behaviour and show its qualitative dynamics under various transmission conditions.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 3","pages":"Article 100278"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148958453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of human antibody specific to the spike glycoprotein subunit 2 of SARS-CoV-2 and characterization of its broad-spectrum neutralizing activity","authors":"Ying Chi,&nbsp;Huimin Yin,&nbsp;Chao Huang,&nbsp;Yiyue Ge,&nbsp;Jingxian Liu,&nbsp;Yuhan Ding,&nbsp;Fengjuan Shi,&nbsp;Zhou Cui,&nbsp;Shuo Ning,&nbsp;Yongjun Jiao,&nbsp;Lunbiao Cui","doi":"10.1016/j.imj.2026.100277","DOIUrl":"10.1016/j.imj.2026.100277","url":null,"abstract":"<div><h3>Background</h3><div>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve and mutate during transmission, making the development of effective broad-spectrum antibodies for the prevention and treatment of SARS-CoV-2 infections of great significance. In this study, peripheral blood mononuclear cells were isolated from 15 convalescent coronavirus disease 2019 (COVID-19) patients to construct a human-derived single-chain variable fragment (scFv) antibody library against SARS-CoV-2. Monoclonal antibodies targeting the conserved structural region of the spike glycoprotein subunit 2 (S2) protein with broad-spectrum neutralizing activity were subsequently screened.</div></div><div><h3>Methods</h3><div>The cDNA of the peripheral blood mononuclear cells from 15 convalescent COVID-19 patients was used to amplify the human-derived antibody heavy chain variable region (VH) and light chain variable region (VL) via polymerase chain reaction (PCR). The VH and VL genes were randomly assembled into scFvs using overlap extension PCR. The constructed scFv gene library was cloned into the phage display vector pComb3XSS and electroporated into XL1-Blue cells to generate a human SARS-CoV-2 scFv antibody library. Then monoclonal antibodies specific to the SARS-CoV-2 S2 domain were screened by phage display. Gene sequencing and recombinant human immunoglobulin G (IgG) monoclonal antibody expression were performed to produce human IgG antibody. The antibody was subsequently evaluated for its binding ability and neutralizing activity against different viral subtypes. Furthermore, the fragment antigen-binding region of the selected antibody was modeled using structure prediction tools to support subsequent structural analysis.</div></div><div><h3>Results</h3><div>The library size of the constructed human-derived SARS-CoV-2 scFv library is 1.6 × 10⁷ CFU. The S2-specific full-length IgG antibody was selected and expressed, which demonstrated neutralizing activity against both the SARS-CoV-2 wild type strain and the Omicron variant.</div></div><div><h3>Conclusions</h3><div>A human single-chain antibody library against SARS-CoV-2 was successfully constructed. From this library, a human antibody targeting the highly conserved S2 region of SARS-CoV-2 was selected, which exhibited broad-spectrum neutralizing activity. This antibody shows potent <em>in vitro</em> neutralizing activity and offers a preliminary molecular basis for the subsequent development of SARS-CoV-2 therapeutics and broad-spectrum vaccines.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 3","pages":"Article 100277"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148958350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A brief review of chikungunya fever: From molecular virology to countermeasures","authors":"Xin Zhang ,&nbsp;Xiaoxi Li ,&nbsp;Tianjun Jiang ,&nbsp;Junliang Fu","doi":"10.1016/j.imj.2025.100231","DOIUrl":"10.1016/j.imj.2025.100231","url":null,"abstract":"<div><div>Chikungunya fever (CHIKF), resulting from the chikungunya virus infection, has become a major global health issue in recent years. This review summarizes the epidemiology, virology, pathogenesis, clinical manifestations, diagnosis, vaccine development and treatment strategies of the CHIKF. And by integrating the findings from various studies, an attempt is made to propose future research directions and intervention strategies.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 1","pages":"Article 100231"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervical brucellar spondylitis: Current management strategies and future directions","authors":"Feng Li ,&nbsp;Xingguo Tan ,&nbsp;Lian Zhang ,&nbsp;Xiaohong Tian ,&nbsp;Songkai Li","doi":"10.1016/j.imj.2025.100234","DOIUrl":"10.1016/j.imj.2025.100234","url":null,"abstract":"<div><div>Cervical brucellar spondylitis (CBS) is a rare but severe form of osteoarticular brucellosis requiring heightened clinical vigilance. This review delineates current management strategies and proposes future directions for CBS, based on a comprehensive analysis of 37 studies (encompassing 101 CBS cases) identified through searches of PubMed, Embase, and the Cochrane Library databases. CBS most commonly manifests with neck pain (occurring in 98% of patients) and neurological deficits (77%). Characteristic imaging findings include T1-weighted hypointensity and T2-weighted hyperintensity affecting the intervertebral discs and adjacent vertebrae, frequently accompanied by epidural abscesses (65.3%), while vertebral bone destruction is less common (30.7%). The cervical segments most frequently involved are C5–C6 (28.7%), followed by C4–C5 (19.8%) and C6–C7 (19.8%). First-line antimicrobial therapy typically involves a combination of doxycycline and rifampin administered for 6–12 weeks. Conservative treatment is the recommended approach for patients without severe neurological deficits, significant bone destruction, or spinal instability. Surgical intervention (utilizing anterior, posterior, or combined approaches) is indicated for cases involving progressive neurological deterioration, radiographic progression, or failure of conservative therapy. Effective management of CBS requires strict adherence to the core principles of early and accurate diagnosis, standardized antimicrobial treatment, and precise intervention. Future strategies should focus on advancing rapid molecular diagnostics, innovating drug therapies and surgical techniques, and enhancing multidisciplinary collaboration to improve clinical outcomes in CBS patients.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 1","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention of mother-to-child transmission of HIV in the MENA region: A systematic review with comparative evidence from Sub-Saharan Africa","authors":"SeyedAhmad SeyedAlinaghi ,&nbsp;Esmaeil Mehraeen ,&nbsp;Sepide Ahmadi ,&nbsp;Soudabeh Yarmohammadi ,&nbsp;Zohal Parmoon ,&nbsp;Amene Abiri ,&nbsp;Mahda Malekshahi ,&nbsp;Ali Moradi ,&nbsp;Soheil Dehghani ,&nbsp;Farid Farahani Rad ,&nbsp;Zahra Soltanali ,&nbsp;Pegah Mirzapour ,&nbsp;Shayesteh Jahanfar","doi":"10.1016/j.imj.2026.100240","DOIUrl":"10.1016/j.imj.2026.100240","url":null,"abstract":"<div><div>The Middle East and North Africa (MENA) region faces distinct challenges in addressing the HIV epidemic, including social stigma, limited surveillance data, and insufficient coverage of prevention services. Prevention of mother-to-child transmission (PMTCT) of HIV remains a critical public health priority, particularly in regions with rising HIV incidence and constrained health systems. We conducted a systematic review to assess the status of PMTCT in the MENA region, identify gaps in effective implementation, and summarize successful interventions and responses. Due to the scarcity of PMTCT studies originating from the MENA region, high-quality studies from Sub-Saharan Africa were also included to provide comparative and contextual evidence relevant to similar resource-limited settings. Electronic databases (PubMed, Web of Science, and Scopus) were searched for articles published up to March 2024, with no restrictions on study design. Methodological quality and risk of bias were assessed using validated tools. A total of 51 studies were included, comprising data from 32,180 HIV-infected mothers and 171,142 infants. The majority of evidence originated from Sub-Saharan Africa, with limited data available from the MENA region. Antiretroviral therapy during pregnancy, particularly highly active antiretroviral therapy (HAART) and lifelong antiretroviral therapy (Option B +), was consistently associated with substantial reductions in mother-to-child transmission (MTCT) rates. Longer duration of antenatal antiretroviral prophylaxis, appropriate infant antiretroviral regimens, and optimized infant-feeding strategies further contributed to improved outcomes. Non-antiretroviral interventions and maternal genetic factors also influenced transmission dynamics. Despite limited region-specific evidence, this review highlights effective PMTCT strategies that are potentially applicable to the MENA region when informed by comparative evidence from Sub-Saharan Africa. Strengthening access to comprehensive and context-adapted PMTCT services, simplifying treatment pathways, and addressing socio-cultural barriers are essential to reducing vertical HIV transmission in the MENA region.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 1","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147379769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nontyphoidal Salmonella encephalopathy in a teenager from Hubei, China","authors":"Hui-Ju Han ,&nbsp;Ya-Ting Zhang ,&nbsp;Jun-Jing Yang ,&nbsp;Wen-Jie Wang ,&nbsp;Jian-Bo Zhan","doi":"10.1016/j.imj.2025.100233","DOIUrl":"10.1016/j.imj.2025.100233","url":null,"abstract":"<div><div>Nontyphoidal <em>Salmonella</em> (NTS) encephalopathy is rare, especially without gastroenteritis. We report a case in a healthy 14-year-old girl presenting with rash and headache, but no diarrhea. Cerebrospinal fluid culture grew Gram-negative bacilli. Genomic sequencing identified a multidrug-resistant <em>Salmonella</em> Typhimurium ST36 strain. The patient progressed to subarachnoid hemorrhage. This case highlights that invasive NTS disease can present atypically in adolescents. NTS should be considered in encephalopathy differentials, irrespective of intestinal symptoms.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 1","pages":"Article 100233"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and genomic characterization of an imported chikungunya virus case in Ningxia Hui Autonomous Region, China, September 2025","authors":"Jianxin Pei ,&nbsp;Jingting Wang ,&nbsp;Ling Niu ,&nbsp;Ting Mu ,&nbsp;Ziyang Luo ,&nbsp;Shanshan Du ,&nbsp;Huiqin Wang ,&nbsp;Yuefen Zhang ,&nbsp;Jiandong Li ,&nbsp;Zhonglan Wu","doi":"10.1016/j.imj.2026.100242","DOIUrl":"10.1016/j.imj.2026.100242","url":null,"abstract":"<div><div>To analyze the first imported case of chikungunya virus (CHIKV) in Ningxia Hui Autonomous Region (Ningxia), China, and to provide insights for the prevention and control of emerging infectious diseases. An epidemiological investigation and viral detection was performed via quantitative reverse transcription polymerase chain reaction (qRT-PCR). The virus was further characterized using whole-genome sequencing and phylogenetic analysis. A 26-year-old female was confirmed to be infected with CHIKV. Genotypic analysis identified the East/Central/South African (ECSA) Indian Ocean Lineage (IOL), specifically the ECSA2 sub-lineage. A key mutation (E1-A226V), known to enhance viral adaptability to <em>Aedes albopictus</em>, was identified. Phylogenetic analysis revealed 99.21% nucleotide similarity to a CHIKV strain isolated in Foshan City, Guangdong Province, in 2025. No <em>Ae. aegypti</em> or <em>Ae. albopictus</em> were found in Ningxia. This imported case of CHIKV in Ningxia, genetically linked to the ECSA-IOL lineage carrying the E1-A226V mutation, underscores the risk of introduction and potential local transmission of arboviruses via domestic travel. Enhanced surveillance, timely diagnosis, and effective management of imported cases are essential for preventing outbreaks.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"5 1","pages":"Article 100242"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}